LRP6 (NM_002336) Human Untagged Clone

CAT#: SC315373

LRP6 (untagged)-Human low density lipoprotein receptor-related protein 6 (LRP6)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: LRP6
• Synonyms: ADCAD2; STHAG7
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_002336 edited
  CTGGGAAGTATGAGCGTGCAGCCCTGCCGCTGCGGCGGCCGCCCCGGCTCCTCGCCTCCC
  CCACTTCTGGCCACCCCTCGCCGGTGAGAGAAGAGAACGCGAGAAGGGAAGATGGGGGCC
  GTCCTGAGGAGCCTCCTGGCCTGCAGCTTCTGTGTGCTCCTGAGAGCGGCCCCTTTGTTG
  CTTTATGCAAACAGACGGGACTTGCGATTGGTTGATGCTACAAATGGCAAAGAGAATGCT
  ACGATTGTAGTTGGAGGCTTGGAGGATGCAGCTGCGGTGGACTTTGTGTTTAGTCATGGC
  TTGATATACTGGAGTGATGTCAGCGAAGAAGCCATTAAACGAACAGAATTTAACAAAACT
  GAGAGTGTGCAGAATGTTGTTGTTTCTGGATTATTGTCCCCCGATGGGCTGGCATGTGAT
  TGGCTTGGAGAAAAATTGTACTGGACAGATTCTGAAACTAATCGGATTGAAGTTTCTAAT
  TTAGATGGATCTTTACGAAAAGTTTTATTTTGGCAAGAGTTGGATCAACCCAGAGCTATT
  GCCTTAGATCCTTCAAGTGGGTTCATGTACTGGACAGACTGGGGAGAAGTGCCAAAGATA
  GAACGTGCTGGAATGGATGGTTCAAGTCGCTTCATTATAATAAACAGTGAAATTTACTGG
  CCAAATGGACTGACTTTGGATTATGAAGAACAAAAGCTTTATTGGGCAGATGCAAAACTT
  AATTTCATCCACAAATCAAATCTGGATGGAACAAATCGGCAGGCAGTGGTTAAAGGTTCC
  CTTCCACATCCTTTTGCCTTGACGTTATTTGAGGACATATTGTACTGGACTGACTGGAGC
  ACACACTCCATTTTGGCTTGCAACAAGTATACTGGTGAGGGTCTGCGTGAAATCCATTCT
  GACATCTTCTCTCCCATGGATATACATGCCTTCAGCCAACAGAGGCAGCCAAATGCCACA
  AATCCATGTGGAATTGACAATGGGGGTTGTTCCCATTTGTGTTTGATGTCTCCAGTCAAG
  CCTTTTTATCAGTGTGCTTGCCCCACTGGGGTCAAACTCCTGGAGAATGGAAAAACCTGC
  AAAGATGGTGCCACAGAATTATTGCTTTTAGCTCGAAGGACAGACTTGAGACGCATTTCT
  TTGGATACACCAGATTTTACAGACATTGTTCTGCAGTTAGAAGACATCCGTCATGCCATT
  GCCATAGATTACGATCCTGTGGAAGGCTACATCTACTGGACTGATGATGAAGTGAGGGCC
  ATACGCCGTTCATTTATAGATGGATCTGGCAGTCAGTTTGTGGTCACTGCTCAAATTGCC
  CATCCTGATGGTATTGCTGTGGACTGGGTTGCACGAAATCTTTATTGGACAGACACTGGC
  ACTGATCGAATAGAAGTGACAAGGCTCAATGGGACCATGAGGAAGATCTTGATTTCAGAG
  GACTTAGAGGAACCCCGGGCTATTGTGTTAGATCCCATGGTTGGGTACATGTATTGGACT
  GACTGGGGAGAAATTCCGAAAATTGAGCGAGCAGCTCTGGATGGTTCTGACCGTGTAGTA
  TTGGTTAACACTTCTCTTGGTTGGCCAAATGGTTTAGCCTTGGATTATGATGAAGGCAAA
  ATATACTGGGGAGATGCCAAAACAGACAAGATTGAGGTTATGAATACTGATGGCACTGGG
  AGACGAGTACTAGTGGAAGACAAAATTCCTCACATATTTGGATTTACTTTGTTGGGTGAC
  TATGTTTACTGGACTGACTGGCAGAGGCGTAGCATTGAAAGAGTTCATAAACGAAGTGCA
  GAGAGGGAAGTGATCATAGATCAGCTGCCTGACCTCATGGGCCTAAAGGCTACAAATGTT
  CATCGAGTGATTGGTTCCAACCCCTGTGCTGAGGAAAACGGGGGATGTAGCCATCTCTGC
  CTCTATAGACCTCAGGGCCTTCGCTGTGCTTGCCCTATTGGCTTTGAACTCATCAGTGAC
  ATGAAGACCTGCATTGTCCCAGAGGCTTTCCTTTTGTTTTCACGGAGAGCAGATATCAGA
  CGAATTTCTCTGGAAACAAACAATAATAATGTGGCTATTCCACTCACTGGTGTCAAAGAA
  GCTTCTGCTTTGGATTTTGATGTGACAGACAACCGAATTTATTGGACTGATATATCACTC
  AAGACCATCAGCAGAGCCTTTATGAATGGCAGTGCACTGGAACATGTGGTAGAATTCGGC
  TTAGATTATCCAGAAGGCATGGCAGTAGACTGGCTTGGGAAGAACTTGTACTGGGCAGAC
  ACAGGAACGAATCGAATTGAGGTGTCAAAGTTGGATGGGCAGCACCGACAAGTTTTGGTG
  TGGAAAGACCTAGATAGTCCCAGAGCTCTCGCGTTGGACCCTGCCGAAGGATTTATGTAT
  TGGACTGAATGGGGTGGAAAACCTAAGATAGACAGAGCTGCAATGGATGGAAGTGAACGT
  ACTACCTTAGTTCCAAATGTGGGGCGGGCAAACGGCCTAACTATTGATTATGCTAAAAGG
  AGGCTTTATTGGACAGACCTGGACACCAACTTAATAGAATCTTCAAATATGCTTGGGCTC
  AACCGTGAAGTTATAGCAGATGACTTGCCTCATCCTTTTGGCTTAACTCAGTACCAAGAT
  TATATCTACTGGACGGACTGGAGCCGACGCAGCATTGAGCGTGCCAACAAAACCAGTGGC
  CAAAACCGCACCATCATTCAGGGCCATTTGGATTATGTGATGGACATCCTCGTCTTTCAC
  TCATCTCGACAGTCAGGGTGGAATGAATGTGCTTCCAGCAATGGGCACTGCTCCCACCTC
  TGCTTGGCTGTGCCAGTTGGGGGTTTTGTTTGTGGATGCCCTGCCCACTACTCTCTTAAT
  GCTGACAACAGGACTTGTAGTGCTCCTACGACTTTCCTGCTCTTCAGTCAAAAGAGTGCC
  ATCAACCGCATGGTGATTGATGAACAACAGAGCCCCGACATCATCCTTCCCATCCACAGC
  CTTCGGAATGTCCGGGCCATTGACTATGACCCACTGGACAAGCAACTCTATTGGATTGAC
  TCACGACAAAACATGATCCGAAAGGCACAAGAAGATGGCAGCCAGGGCTTTACTGTGGTT
  GTGAGCTCAGTTCCGAGTCAGAACCTGGAAATACAACCCTATGACCTCAGCATTGATATT
  TACAGCCGCTACATCTACTGGACTTGTGAGGCTACCAATGTCATTAATGTGACAAGATTA
  GATGGGAGATCAGTTGGAGTGGTGCTGAAAGGCGAGCAGGACAGACCTCGAGCCATTGTG
  GTAAACCCAGAGAAAGGGTATATGTATTTTACCAATCTTCAGGAAAGGTCTCCTAAAATT
  GAACGGGCTGCTTTGGATGGGACAGAACGGGAGGTCCTCTTTTTCAGTGGCTTAAGTAAA
  CCAATTGCTTTAGCCCTTGATAGCAGGCTGGGCAAGCTCTTTTGGGCTGATTCAGATCTC
  CGGCGAATTGAAAGCAGTGATCTCTCAGGTGCTAACCGGATAGTATTAGAAGACTCCAAT
  ATCTTGCAGCCTGTGGGACTTACTGTGTTTGAAAACTGGCTCTATTGGATTGATAAACAG
  CAGCAAATGATTGAAAAAATTGACATGACAGGTCGAGAGGGTAGAACCAAAGTCCAAGCT
  CGAATTGCCCAGCTTAGTGACATTCATGCAGTAAAGGAGCTGAACCTTCAAGAATACAGA
  CAGCACCCTTGTGCTCAGGATAATGGTGGCTGTTCACATATTTGTCTTGTAAAGGGGGAT
  GGTACTACAAGGTGTTCTTGCCCCATGCACCTGGTTCTACTTCAAGATGAGCTATCATGT
  GGAGAACCTCCAACATGTTCTCCTCAGCAGTTTACTTGTTTCACGGGGGAAATTGACTGT
  ATCCCTGTGGCTTGGCGGTGCGATGGGTTTACTGAATGTGAAGACCACAGTGATGAACTC
  AATTGTCCTGTATGCTCAGAGTCCCAGTTCCAGTGTGCCAGTGGGCAGTGTATTGATGGT
  GCCCTCCGATGCAATGGAGATGCAAACTGCCAGGACAAATCAGATGAGAAGAACTGTGAA
  GTGCTTTGTTTAATTGATCAGTTCCGCTGTGCCAATGGTCAGTGCATTGGAAAGCACAAG
  AAGTGTGATCATAATGTGGATTGCAGTGACAAGTCAGATGAACTGGATTGTTATCCGACT
  GAAGAACCAGCACCACAGGCCACCAATACAGTTGGTTCTGTTATTGGCGTAATTGTCACC
  ATTTTTGTGTCTGGAACTGTATACTTTATCTGCCAGAGGATGTTGTGTCCACGTATGAAG
  GGAGATGGGGAAACTATGACTAATGACTATGTAGTTCATGGACCAGCTTCTGTGCCTCTT
  GGTTATGTGCCACACCCAAGTTCTTTGTCAGGATCTCTTCCAGGAATGTCTCGAGGTAAA
  TCAATGATCAGCTCCCTCAGTATCATGGGGGGAAGCAGTGGACCCCCCTATGACCGAGCC
  CATGTTACAGGAGCATCATCAAGTAGTTCTTCAAGCACCAAAGGCACTTACTTCCCTGCA
  ATTTTGAACCCTCCACCATCCCCAGCCACAGAGCGATCACATTACACTATGGAATTTGGA
  TATTCTTCAAACAGTCCTTCCACTCATAGGTCATACAGCTACAGGCCATATAGCTACCGG
  CACTTTGCACCCCCCACCACACCCTGCAGCACAGATGTTTGTGACAGTGACTATGCTCCT
  AGTCGGAGAATGACCTCAGTGGCAACAGCCAAGGGCTATACCAGTGACTTGAACTATGAT
  TCAGAACCTGTGCCCCCACCTCCCACACCCCGAAGCCAATACTTGTCAGCAGAGGAGAAC
  TATGAAAGCTGCCCACCTTCTCCATACACAGAGAGGAGCTATTCTCATCACCTCTACCCA
  CCGCCACCCTCTCCCTGTACAGACTCCTCCTGAGGAGGGGCCCTCCTCCTCTGACTGCCT
  CCAACGTAAAAATGTAAATATAAATTTGGTTGAGATCTGGAGGGGGGGAGGGAGCTATTA
  GAGAAGGATGAGGCAGACCATGTACAGTTAAAATTATAAAATGGGGTAGGGAATACTGGA
  GATATTTGTACAGAAGAAAAGGATATTTATATATTTTCTTAAAACAGCAGATTTGCTGCT
  TGTGCCATAAAAGTTTGTATAAAAAAAATTTGTACTAAAAGTTTTATTTTTGCAAACTAA
  ATACACAAAGCATGCCTTAAACCCAGTGAAGCAACTGAGTACAAAGGAAACAGGAATAAT
  AAAGGCATCACTGACCAGGAATATCTGGGCTTTATTGATACCAAAAATAAAAAAAAAAAA
  AAAAAA
  
  5' Read Nucleotide Sequence

  >Reverse primer walk for NM_002336 unedited
  GCCTAGTTCATAAACTTTTCTAAACTCCTCTAATTAGAAACTTCATCCGATTAGTTTCAG
  AATCTGTCCAGTACAATTTTTCTCCAAGCCAATCACATGCCAGCCCATCGGGGGACAATA
  ATCCAGAAACAACAACATTCTGCACACTCTCAGTTTTGTTAAATTCTGTTCGTTTAATGG
  CTTCTTCGCTGACATCACTCCAGTATATCAAGCCATGACTAAACACAAAGTCCACCGCAG
  CTGCATCCTCCAAGCCTCCAACTACAATCGTAGCATTCTCTTTGCCATTTGTAGCATCAA
  CCAATCGCAAGTCCCGTCTGTTTGCATAAAGCAACAAAGGGGCCGCTCTCAGGAGCACAC
  AGAAGCTGCAGGCCAGGAGGCTCCTCAGGACGGCCCCCATCTTCCCTTCTCGCGTTCTCT
  TCTCTCACCGGCGAGGGGTGGCCAGAAGTGGGGGAGGCGAGGAGCCGGGGCGGCCGCCGC
  AGCGGCAGGGCTGCACGCTCATACTTCCCAGCCTCGTGCCGAATTCGCGGCCGCCCTATA
  GTGAGTCGTATTACANAATTCTGACGGTTCACTAAACGAGCTCTGCTTATATAGACCTCC
  CACCGTACACGCCTACCGCCCATTTGCGTCAACGGGGCGGGGTTATTACGACATTTTGGA
  AGTCCNGTTGATTTTGGTGCCAAACANAACTCCATTGACGTCNAATGGGTGGAGACTTGG
  AAATCCCCGTGAGTCAAACCGCTATCCACGCCCATTGGTGTACTGCCAAAACCGCATCAC
  CATGGTAATAGCGATGACTAATACGTAGATGTACTGCCCAGTAAGAAAGTCCCGTAAGGT
  CATGTACTGGGCATAATGCCAGGCGGGCCATTTACCGTCTTTGACGTAAC
  
  3' Read Nucleotide Sequence

  >Reverse primer walk for NM_002336 unedited
  GGATCTATTACTTTTTACCTGGTAGCATCANAGNAGGAGGCCCCTCCTCAGNAGAGTCTG
  TACAGGGAGAGGGTGGCGGTGGGTAGAGGTGATGAGAATAGCTCCTCTCTGTGTATGGAG
  AAGGTGGGCAGCTTTCATAGTTCTCCTCTGCTGACAAGTATTGGCTTCGGGGTGTGGGAG
  GTGGGGGCACAGGTTCTGAATCATAGTTCAAGTCACTGGTATAGCCCTTGGCTGTTGCCA
  CTGAGGTCATTCTCCGACTAGGAGCATAGTCACTGTCACAAACATCTGTGCTGCAGGGTG
  TGGTGGGGGGTGCAAAGTGCCGGTAGCTATATGGCCTGTAGCTGTATGACCTATGAGTGG
  AAGGACTGTTTGAAGAATATCCAAATTCCATAGTGTAATGTGATCGCTCTGTGGCTGGGG
  ATGGTGGAGGGTTCAAAATTGCAGGGAAGTAAGTGCCTTTGGTGCTTGAAGAACTACTTG
  ATGATGCTCCTGTAACATGGGCTCGGTCATAGGGGGGTCCACTGCTTCCCCCCATGATAC
  TGAGGGAGCTGATCATTGATTTACCTCGAGACATTCCTGGAAGAGATCCTGACAAAGAAC
  TTGNGTGTGGCACATAACCAAGAGGCACAGAAGCTGGTCCATGAACTACATAGTCATTAG
  TCATAGTTTCCCCCTCTCCCTTCATACGTGGACACAACATCCTCTGNCAGATAAAGTATA
  CAGTTCCAGACCCAAAAATGGTGACAATTACGCCAATACCGAACCAACTGTATTGGTGGC
  CTGTGGTGCTTGGTTCTCCATCGGAAAACATCCCATTCAATCTGCCTGTCCCTGCAATCC
  CCTTAAGATCCCCCTCCTGGGGCTTTCCATGCCCCTGCCATTGGCACCACGG
  
• Restriction Sites: Please inquire
• ACCN: NM_002336
• Insert Size: 5300 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002336.1, NP_002327.1
• RefSeq Size: 5301 bp
• RefSeq ORF: 4842 bp
• Locus ID: 4040
• UniProt ID: O75581
• Cytogenetics: 12p13.2
• Domains: ldl_recept_b, ldl_recept_a, EGF, EGF
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Wnt signaling pathway
• Gene Summary: This gene encodes a member of the low density lipoprotein (LDL) receptor gene family. LDL receptors are transmembrane cell surface proteins involved in receptor-mediated endocytosis of lipoprotein and protein ligands. The protein encoded by this gene functions as a receptor or, with Frizzled, a co-receptor for Wnt and thereby transmits the canonical Wnt/beta-catenin signaling cascade. Through its interaction with the Wnt/beta-catenin signaling cascade this gene plays a role in the regulation of cell differentiation, proliferation, and migration and the development of many cancer types. This protein undergoes gamma-secretase dependent RIP- (regulated intramembrane proteolysis) processing but the precise locations of the cleavage sites have not been determined.[provided by RefSeq, Dec 2009]