RNF123 (NM_022064) Human Untagged Clone

CAT#: SC315350

RNF123 (untagged)-Human ring finger protein 123 (RNF123)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RNF123
• Synonyms: FP1477; KPC1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC315350 representing NM_022064.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGCATCCAAGGGGGCCGGCATGTCTTTCTCCCGCAAGAGCTATAGGCTGACCTCAGATGCTGAGAAA
  TCCAGGGTCACAGGCATTGTGCAGGAGAAGCTGCTGAATGACTACCTGAACCGCATCTTTTCCTCTTCT
  GAACATGCACCCCCAGCAGCCACCAGCAGGAAACCCCTGAACTTCCAGAACCTGCCAGAACATTTGGAC
  CAGTTGCTACAGGTGGACAATGAGGAGGAGGAAAGCCAGGGACAGGTTGAAGGGCGGCTTGGCCCATCC
  ACTGTGGTCCTGGACCACACAGGCGGCTTTGAGGGGCTTCTCCTGGTGGATGATGACCTGCTGGGGGTG
  ATTGGACACAGCAACTTTGGCACCATCCGCTCTACCACATGCGTGTACAAAGGGAAATGGCTCTACGAG
  GTCCTCATCTCCTCCCAGGGGCTCATGCAGATCGGCTGGTGCACCATCAGCTGCCGCTTCAACCAGGAG
  GAGGGGGTTGGAGATACACACAACTCCTATGCCTATGATGGCAACCGCGTGCGCAAGTGGAATGTGACC
  ACAACGAATTATGGCAAGGCGTGGGCAGCGGGGGACATCGTGAGCTGCCTGATTGACCTGGATGATGGC
  ACTCTGTCCTTCTGCCTGAACGGTGTATCACTGGGCACTGCCTTTGAGAACCTGTCCAGGGGCCTGGGT
  ATGGCCTACTTCCCAGCCATCAGCCTCTCTTTCAAGGAGTCCGTGGCCTTCAACTTTGGCAGCCGTCCT
  CTGCGCTACCCAGTGGCAGGCTACCGGCCCCTGCAGGACCCACCGAGTGCTGACCTGGTGCGGGCACAG
  AGGTTGCTGGGCTGCTTCCGGGCAGTGCTGAGTGTGGAGCTGGACCCTGTGGAGGGGCGGCTGTTGGAC
  AAGGAGAGCTCCAAGTGGCGGTTGCGGGGCCAGCCCACCGTCCTCCTCACACTGGCCCACATCTTCCAT
  CACTTTGCACCGCTTCTGCGCAAGGTGTATCTGGTGGAGGCTGTGCTCATGAGCTTCTTGCTGGGCATC
  GTGGAGAAGGGCACACCCACACAGGCACAGTCCGTGGTGCACCAGGTCCTGGACCTCTTGTGGCTCTTC
  ATGGAGGACTACGAGGTACAAGATTGCCTCAAGCAGTTGATGATGTCTCTGCTTCGGCTGTACCGATTC
  TCACCCATTGTCCCAGACCTGGGCCTACAGATCCATTACCTGCGGCTCACTATCGCCATCCTGAGGCAT
  GAGAAGTCCCGCAAGTTTCTGCTTAGCAATGTCCTCTTCGACGTGCTCCGCTCCGTCGTCTTCTTTTAC
  ATCAAGAGCCCCCTGCGTGTGGAGGAGGCCGGCCTGCAGGAGCTCATTCCCACCACCTGGTGGCCCCAC
  TGCTCCAGTAGGGAGGGCAAAGAGAGCACGGAGATGAAGGAGGAGACCGCAGAGGAGCGGCTGCGGCGG
  CGAGCCTACGAACGGGGCTGTCAGCGGCTCAGGAAGCGCATCGAAGTGGTGGAAGAACTACAGGTCCAG
  ATCCTGAAGCTGCTGCTGGACAATAAAGATGACAATGGGGGTGAAGCTTCTAGGTATATCTTCCTGACC
  AAGTTTCGCAAGTTTCTGCAGGAGAACGCCAGTGGCCGGGGGAACATGCCCATGCTCTGCCCCCCTGAG
  TACATGGTCTGCTTCTTACACCGGCTGATCTCTGCCCTGCGCTACTATTGGGATGAATACAAGGCTTCC
  AATCCTCATGCTTCCTTCAGTGAGGAGGCCTACATCCCGCCCCAGGTCTTCTATAATGGCAAGGTGGAC
  TACTTTGACCTGCAGCGCCTGGGGGGCCTCCTCTCGCACCTGCGGAAGACCCTCAAAGATGACCTTGCT
  TCCAAAGCCAACATTGTGATCGACCCACTGGAGCTCCAGTCAACCGCCATGGATGACCTAGATGAGGAT
  GAGGAGCCAGCCCCAGCTATGGCCCAGCGCCCCATGCAGGCCCTGGCTGTTGGGGGGCCACTGCCCCTG
  CCCCGGCCCGGCTGGCTCAGTTCTCCAACTTTGGGCCGAGCCAACCGCTTCCTCAGCACAGCGGCTGTG
  AGCCTCATGACCCCACGGCGGCCTCTGAGCACCTCGGAGAAAGTGAAGGTCCGCACGCTGAGCGTGGAG
  CAGAGGACCCGTGAGGACATTGAAGGCAGCCACTGGAATGAGGGCTTGCTGCTGGGGCGGCCCCCCGAG
  GAGCCTGAGCAGCCCCTCACCGAGAACTCGCTGCTGGAAGTCCTGGATGGGGCGGTCATGATGTACAAC
  CTCAGCGTACACCAGCAGCTGGGCAAGATGGTGGGTGTCTCCGATGATGTCAATGAATACGCTATGGCT
  CTGAGGGACACAGAGGACAAGCTCCGCCGGTGCCCCAAGAGGAGGAAGGACATCCTTGCAGAGTTGACC
  AAGAGCCAGAAGGTTTTCTCAGAAAAGCTGGACCACCTGAGCCGCCGTCTTGCCTGGGTCCATGCCACT
  GTCTACTCCCAGGAGAAGATGCTGGACATCTACTGGCTGCTGCGCGTCTGCCTGCGGACCATTGAGCAC
  GGTGATCGCACAGGGTCTCTCTTTGCCTTCATGCCCGAGTTCTACCTGAGCGTGGCCATCAACAGCTAC
  AGTGCTCTCAAGAATTACTTTGGTCCCGTGCACAGCATGGAGGAGCTCCCAGGCTATGAAGAGACCCTG
  ACCCGCCTGGCTGCCATTCTCGCCAAACACTTTGCCGACGCACGCATTGTGGGCACTGACATCCGAGAC
  TCACTGATGCAGGCCCTGGCCAGCTACGTGTGCTACCCACACTCCCTGCGGGCTGTGGAGCGAATCCCC
  GAGGAGCAGCGTATCGCCATGGTGAGGAACCTCCTGGCGCCCTATGAGCAGCGGCCCTGGGCCCAGACC
  AACTGGATCCTGGTGCGGCTCTGGAGGGGCTGTGGCTTCGGGTACCGCTATACACGGCTGCCACATCTG
  CTGAAAACCAAACTTGAGGACGCCAATTTGCCCAGCCTCCAGAAGCCCTGCCCTTCCACCCTGCTGCAG
  CAGCACATGGCGGACCTCCTACAGCAGGGTCCTGATGTGGCACCCAGCTTCCTCAACAGCGTCCTCAAT
  CAGCTCAACTGGGCCTTCTCTGAATTCATTGGCATGATCCAAGAGATCCAGCAGGCTGCTGAGCGCCTG
  GAGCGGAACTTTGTGGACAGCCGGCAGCTCAAGGTATGTGCCACCTGCTTTGACCTCTCGGTCAGCCTG
  CTGCGTGTCTTGGAGATGACTATCACACTGGTGCCTGAGATATTCCTTGACTGGACCCGGCCTACCTCT
  GAGATGCTGCTGCGGCGTCTTGCACAGCTGCTAAACCAGGTGCTGAACCGGGTGACAGCTGAGAGGAAC
  CTGTTTGATCGTGTGGTCACCCTACGGCTGCCTGGCCTAGAGAGCGTGGACCACTATCCCATTCTGGTG
  GCAGTGACGGGCATCCTGGTGCAGCTCCTGGTGCGTGGCCCAGCCTCAGAGAGAGAGCAAGCCACATCA
  GTGCTCCTGGCAGATCCCTGCTTCCAGCTACGCTCAATATGCTATCTCCTGGGACAGCCAGAGCCCCCA
  GCACCTGGCACTGCTCTGCCAGCCCCTGACCGGAAGCGCTTCTCCCTGCAGAGCTATGCGGATTATATC
  AGTGCCGATGAGCTGGCCCAAGTGGAACAGATGCTGGCGCACCTGACCTCTGCATCTGCCCAGGCAGCA
  GCTGCCTCCCTGCCCACCAGTGAGGAGGACCTCTGCCCCATCTGCTATGCCCACCCCATCTCTGCTGTG
  TTCCAGCCCTGTGGCCACAAGTCCTGCAAAGCCTGTATCAACCAGCACCTGATGAACAACAAGGACTGC
  TTCTTCTGCAAAACCACCATCGTGTCTGTAGAGGACTGGGAGAAGGGAGCCAATACGAGTACTACCTCC
  TCAGCTGCCTAG
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_022064
• Insert Size: 3945 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_022064.4
• RefSeq Size: 4330 bp
• RefSeq ORF: 3945 bp
• Locus ID: 63891
• UniProt ID: Q5XPI4
• Cytogenetics: 3p21.31
• Domains: RING
• Protein Families: Druggable Genome
• MW: 148.5 kDa
• Gene Summary: The protein encoded by this gene contains a C-terminal RING finger domain, a motif present in a variety of functionally distinct proteins and known to be involved in protein-protein and protein-DNA interactions, and an N-terminal SPRY domain. This protein displays E3 ubiquitin ligase activity toward the cyclin-dependent kinase inhibitor 1B which is also known as p27 or KIP1. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016]
  Transcript Variant: This variant (1) encodes the supported protein.