ADCY6 (NM_020983) Human Untagged Clone

CAT#: SC315194

ADCY6 (untagged)-Human adenylate cyclase 6 (ADCY6), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ADCY6
• Synonyms: AC6; LCCS8
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC315194 representing NM_020983.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGTCATGGTTTAGTGGCCTCCTGGTCCCTAAAGTGGATGAACGGAAAACAGCCTGGGGTGAACGCAAT
  GGGCAGAAGCGTTCGCGGCGCCGTGGCACTCGGGCAGGTGGCTTCTGCACGCCCCGCTATATGAGCTGC
  CTCCGGGATGCAGAGCCACCCAGCCCCACCCCTGCGGGCCCCCCTCGGTGCCCCTGGCAGGATGACGCC
  TTCATCCGGAGGGGCGGCCCAGGCAAGGGCAAGGAGCTGGGGCTGCGGGCAGTGGCCCTGGGCTTCGAG
  GATACCGAGGTGACAACGACAGCGGGCGGGACGGCTGAGGTGGCGCCCGACGCGGTGCCCAGGAGTGGG
  CGATCCTGCTGGCGCCGTCTGGTGCAGGTGTTCCAGTCGAAGCAGTTCCGTTCGGCCAAGCTGGAGCGC
  CTGTACCAGCGGTACTTCTTCCAGATGAACCAGAGCAGCCTGACGCTGCTGATGGCGGTGCTGGTGCTG
  CTCACAGCGGTGCTGCTGGCTTTCCACGCCGCACCCGCCCGCCCTCAGCCTGCCTATGTGGCACTGTTG
  GCCTGTGCCGCCGCCCTGTTCGTGGGGCTCATGGTGGTGTGTAACCGGCATAGCTTCCGCCAGGACTCC
  ATGTGGGTGGTGAGCTACGTGGTGCTGGGCATCCTGGCGGCAGTGCAGGTCGGGGGCGCTCTCGCAGCA
  GACCCGCGCAGCCCCTCTGCGGGCCTCTGGTGCCCTGTGTTCTTTGTCTACATCGCCTACACGCTCCTC
  CCCATCCGCATGCGGGCTGCCGTCCTCAGCGGCCTGGGCCTCTCCACCTTGCATTTGATCTTGGCCTGG
  CAACTTAACCGTGGTGATGCCTTCCTCTGGAAGCAGCTCGGTGCCAATGTGCTGCTGTTCCTCTGCACC
  AACGTCATTGGCATCTGCACACACTATCCAGCAGAGGTGTCTCAGCGCCAGGCCTTTCAGGAGACCCGC
  GGTTACATCCAGGCCCGGCTCCACCTGCAGCATGAGAATCGGCAGCAGGAGCGGCTGCTGCTGTCGGTA
  TTGCCCCAGCACGTTGCCATGGAGATGAAAGAAGACATCAACACAAAAAAAGAAGACATGATGTTCCAC
  AAGATCTACATACAGAAGCATGACAATGTCAGCATCCTGTTTGCAGACATTGAGGGCTTCACCAGCCTG
  GCATCCCAGTGCACTGCGCAGGAGCTGGTCATGACCCTGAATGAGCTCTTTGCCCGGTTTGACAAGCTG
  GCTGCGGAGAATCACTGCCTGAGGATCAAGATCTTGGGGGACTGTTACTACTGTGTGTCAGGGCTGCCG
  GAGGCCCGGGCCGACCATGCCCACTGCTGTGTGGAGATGGGGGTAGACATGATTGAGGCCATCTCGCTG
  GTACGTGAGGTGACAGGTGTGAATGTGAACATGCGCGTGGGCATCCACAGCGGGCGCGTGCACTGCGGC
  GTCCTTGGCTTGCGGAAATGGCAGTTCGATGTGTGGTCCAATGATGTGACCCTGGCCAACCACATGGAG
  GCAGGAGGCCGGGCTGGCCGCATCCACATCACTCGGGCAACACTGCAGTACCTGAACGGGGACTACGAG
  GTGGAGCCAGGCCGTGGTGGCGAGCGCAACGCGTACCTCAAGGAGCAGCACATTGAGACTTTCCTCATC
  CTGGGCGCCAGCCAGAAACGGAAAGAGGAGAAGGCCATGCTGGCCAAGCTGCAGCGGACTCGGGCCAAC
  TCCATGGAAGGGCTGATGCCGCGCTGGGTTCCTGATCGTGCCTTCTCCCGGACCAAGGACTCCAAGGCC
  TTCCGCCAGATGGGCATTGATGATTCCAGCAAAGACAACCGGGGCACCCAAGATGCCCTGAACCCTGAG
  GATGAGGTGGATGAGTTCCTGAGCCGTGCCATCGATGCCCGCAGCATTGATCAGCTGCGGAAGGACCAT
  GTGCGCCGGTTTCTGCTCACCTTCCAGAGAGAGGATCTTGAGAAGAAGTACTCCCGGAAGGTGGATCCC
  CGCTTCGGAGCCTACGTTGCCTGTGCCCTGTTGGTCTTCTGCTTCATCTGCTTCATCCAGCTTCTCATC
  TTCCCACACTCCACCCTGATGCTTGGGATCTATGCCAGCATCTTCCTGCTGCTGCTAATCACCGTGCTG
  ATCTGTGCTGTGTACTCCTGTGGTTCTCTGTTCCCTAAGGCCCTGCAACGTCTGTCCCGCAGCATTGTC
  CGCTCACGGGCACATAGCACCGCAGTTGGCATCTTTTCCGTCCTGCTTGTGTTTACTTCTGCCATTGCC
  AACATGTACTTCATCGGGAACATGCTGCTGAGTCTCTTGGCCAGCTCTGTCTTCCTGCACATCAGCAGC
  ATCGGGAAGTTGGCCATGATCTTTGTCTTGGGGCTCATCTATTTGGTGCTGCTTCTGCTGGGTCCCCCA
  GCCACCATCTTTGACAACTATGACCTACTGCTTGGCGTCCATGGCTTGGCTTCTTCCAATGAGACCTTT
  GATGGGCTGGACTGTCCAGCTGCAGGGAGGGTGGCCCTCAAATATATGACCCCTGTGATTCTGCTGGTG
  TTTGCGCTGGCGCTGTATCTGCATGCTCAGCAGGTGGAGTCGACTGCCCGCCTAGACTTCCTCTGGAAA
  CTACAGGCAACAGGGGAGAAGGAGGAGATGGAGGAGCTACAGGCATACAACCGGAGGCTGCTGCATAAC
  ATTCTGCCCAAGGACGTGGCGGCCCACTTCCTGGCCCGGGAGCGCCGCAATGATGAACTCTACTATCAG
  TCGTGTGAGTGTGTGGCTGTTATGTTTGCCTCCATTGCCAACTTCTCTGAGTTCTATGTGGAGCTGGAG
  GCAAACAATGAGGGTGTCGAGTGCCTGCGGCTGCTCAACGAGATCATCGCTGACTTTGATGAGATTATC
  AGCGAGGAGCGGTTCCGGCAGCTGGAAAAGATCAAGACGATTGGTAGCACCTACATGGCTGCCTCAGGG
  CTGAACGCCAGCACCTACGATCAGGTGGGCCGCTCCCACATCACTGCCCTGGCTGACTACGCCATGCGG
  CTCATGGAGCAGATGAAGCACATCAATGAGCACTCCTTCAACAATTTCCAGATGAAGATTGGGCTGAAC
  ATGGGCCCAGTCGTGGCAGGTGTCATCGGGGCTCGGAAGCCACAGTATGACATCTGGGGGAACACAGTG
  AATGTCTCTAGTCGTATGGACAGCACGGGGGTCCCCGACCGAATCCAGGTGACCACGGACCTGTACCAG
  GTTCTAGCTGCCAAGGGCTACCAGCTGGAGTGTCGAGGGGTGGTCAAGGTGAAGGGCAAGGGGGAGATG
  ACCACCTACTTCCTCAATGGGGGCCCCAGCAGTTAA
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGAT
  ATCCTGGATTACAAGGATGACGACGATAAGGTTTAA
  
• Restriction Sites: SgfI-RsrII
• ACCN: NM_020983
• Insert Size: 3348 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020983.2
• RefSeq Size: 5877 bp
• RefSeq ORF: 3348 bp
• Locus ID: 112
• Cytogenetics: 12q13.12
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Chemokine signaling pathway, Dilated cardiomyopathy, Gap junction, GnRH signaling pathway, Melanogenesis, Oocyte meiosis, Progesterone-mediated oocyte maturation, Purine metabolism, Taste transduction, Vascular smooth muscle contraction
• MW: 124.9 kDa
• Gene Summary: This gene encodes a member of the adenylyl cyclase family of proteins, which are required for the synthesis of cyclic AMP. All members of this family have an intracellular N-terminus, a tandem repeat of six transmembrane domains separated by a cytoplasmic loop, and a C-terminal cytoplasmic domain. The two cytoplasmic regions bind ATP and form the catalytic core of the protein. Adenylyl cyclases are important effectors of transmembrane signaling pathways and are regulated by the activity of G protein coupled receptor signaling. This protein belongs to a small subclass of adenylyl cyclase proteins that are functionally related and are inhibited by protein kinase A, calcium ions and nitric oxide. A mutation in this gene is associated with arthrogryposis multiplex congenita. [provided by RefSeq, May 2015]
  Transcript Variant: This variant (2) has a different and shorter 5' UTR and lacks an in-frame fragment of 159 bp in the coding region, as compared to variant 1. Isoform b encoded by this variant has the same N- and C- termini as isoform a encoded by variant 1, but lacks internal 53 aa.