STIM2 (NM_020860) Human Untagged Clone

SKU SC314792

STIM2 (untagged)-Human stromal interaction molecule 2 (STIM2), transcript variant 2

Product Data

• Type: Human Untagged Clone
• Target Symbol: STIM2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_020860 edited; GGGGGATGCGCCGCGGCGGCGGCGGCGCGGGAGCTGGGGTTGGTGTTTGGCGGCGCCAGA; GCAGCGGATCCCGGTCTCGCCGCAGCAGCAGCGCGGGTGTCGTGCACCGCCTGAAGACGC; CGTACCTTTCTACCCCCCACCTTTTTTTTTTTTTTTTTTTAAATAACCGGAACCAATGAA; CGCAGCCGGGATCAGAGCTCCGGAGGCCGCCGGTGCCGATGGGACCAGGCTGGCGCCCGG; CGGGAGCCCGTGTCTGAGGCGGCGGGGGCGGCCGGAGGAGTCGCCGGCGGCGGTGGTGGC; GCCTCGCGGAGCCGGCGAGCTGCAGGCGGCCGGGGCGCCGCTGCGCTTTCACCCGGCTTC; TCCTCGGCGCCTTCATCCCGCCTCGACTCCTGGCCCAGCGTGGGGCTGGCTGCTGCGGCG; GCGGCGCTGGGCTGCGTTGCTGGTGCTCGGGCTGCTGGTAGCCGGAGCGGCGGACGGATG; CGAGCTTGTGCCCCGGCACCTCCGCGGGCGGCGGGCGACTGGCTCTGCCGCAACTGCCGC; CTCCTCTCCCGCCGCGGCGGCCGGCGATAGCCCGGCGCTCATGACAGATCCCTGCATGTC; ACTGAGTCCACCATGCTTTACAGAAGAAGACAGATTTAGTCTGGAAGCTCTTCAAACAAT; ACATAAACAAATGGATGATGACAAAGATGGTGGAATTGAAGTAGAGGAAAGTGATGAATT; CATCAGAGAAGATATGAAATATAAAGATGCTACTAATAAACACAGCCATCTGCACAGAGA; AGATAAACATATAACGATTGAGGATTTATGGAAACGATGGAAAACATCAGAAGTTCATAA; TTGGACCCTTGAAGACACTCTTCAGTGGTTGATAGAGTTTGTTGAACTACCCCAATATGA; GAAGAATTTTAGAGACAACAATGTCAAAGGAACGACACTTCCCAGGATAGCAGTGCACGA; ACCTTCATTTATGATCTCCCAGTTGAAAATCAGTGACCGGAGTCACAGACAAAAACTTCA; GCTCAAGGCATTGGATGTGGTTTTGTTTGGACCTCTAACACGCCCACCTCATAACTGGAT; GAAAGATTTTATCCTCACAGTTTCTATAGTAATTGGTGTTGGAGGCTGCTGGTTTGCTTA; TACGCAGAATAAGACATCAAAAGAACATGTTGCAAAAATGATGAAAGATTTAGAGAGCTT; ACAAACTGCAGAGCAAAGTCTAATGGACTTACAAGAGAGGCTTGAAAAGGCACAGGAAGA; AAACAGAAATGTTGCTGTAGAAAAGCAAAATTTAGAGCGCAAAATGATGGATGAAATCAA; TTATGCAAAGGAGGAGGCTTGTCGGCTGAGAGAGCTAAGGGAGGGAGCTGAATGTGAATT; GAGTAGACGTCAGTATGCAGAACAGGAATTGGAACAGGTTCGCATGGCTCTGAAAAAGGC; CGAAAAAGAATTTGAACTGAGAAGCAGTTGGTCTGTTCCAGATGCACTTCAGAAATGGCT; TCAGTTAACACATGAAGTAGAAGTGCAATACTACAATATTAAAAGACAAAACGCTGAAAT; GCAGCTAGCTATTGCTAAAGATGAGGCAGAAAAAATTAAAAAGAAGAGAAGCACAGTCTT; TGGGACTCTGCACGTTGCACACAGCTCCTCCCTAGATGAGGTAGACCACAAAATTCTGGA; AGCAAAGAAAGCTCTCTCTGAGTTGACAACTTGTTTACGAGAACGACTTTTTCGCTGGCA; ACAAATTGAGAAGATCTGTGGCTTTCAGATAGCCCATAACTCAGGACTCCCCAGCCTGAC; CTCTTCCCTTTATTCTGATCACAGCTGGGTGGTGATGCCCAGAGTCTCCATTCCACCCTA; TCCAATTGCTGGAGGAGTTGATGACTTAGATGAAGACACACCCCCAATAGTGTCACAATT; TCCCGGGACCATGGCTAAACCTCCTGGATCATTAGCCAGAAGCAGCAGCCTGTGCCGTTC; ACGCCGCAGCATTGTGCCGTCCTCGCCTCAGCCTCAGCGAGCTCAGCTTGCTCCACACGC; CCCCCACCCGTCACACCCTCGGCACCCTCACCACCCGCAACACACACCACACTCCTTGCC; TTCCCCTGATCCAGATATCCTCTCAGTGTCAAGTTGCCCTGCGCTTTATCGAAATGAAGA; GGAGGAAGAGGCCATTTACTTCTCTGCTGAAAAGCAATGGGAAGTGCCAGACACAGCTTC; AGAATGTGACTCCTTAAATTCTTCCATTGGAAGGAAACAGTCTCCTCCTTTAAGCCTCGA; GATATACCAAACATTATCTCCGCGAAAGATATCAAGAGATGAGGTGTCCCTAGAGGATTC; CTCCCGAGGGGATTCGCCTGTAACTGTGGATGTGTCTTGGGGTTCTCCCGACTGTGTAGG; TCTGACAGAAACTAAGAGTATGATCTTCAGTCCTGCAAGCAAAGTGTACAATGGCATTTT; GGAGAAATCCTGTAGCATGAACCAGCTTTCCAGTGGCATCCCGGTGCCTAAACCTCGCCA; CACATCATGTTCCTCAGCTGGCAACGACAGTAAACCAGTTCAGGAAGCCCCAAGTGTTGC; CAGAATAAGCAGCATCCCACATGACCTTTGTCATAATGGAGAGAAAAGCAAAAAGCCATC; AAAAATCAAAAGCCTTTTTAAGAAGAAATCTAAGTGAACTGGCTGACTTGATGGAATCAT; GTTCAAGTGGCATCTGTAAACTATTATCCCCCACCCTCCACTCCCCACCTTTTTTTTGGT; TTAATTTTAGGAATGTAACTCCATTGGGGCTTTCCAGGCCGGATGCCATAGTGGAACATC; CAGAAGGGCAACTGTCTACTGTCTGCTTATTTAAGTGACTATATATAATCAATTCATCAA; GCCAGTTATTACTGAAAAATCATTGAAATGAGACAGTTTACAGTCATTTCTGCCTATTTA; TTTCTGCTTTGTTCTCAGTGATGTATATGCAACATTTTGTTGAAAGCCACGATGGACTTA; CAAGCTTTAATGGACTCGTAAGCCAGCATGGGCTTGCAAAAATTTCTTGTTTACCAGAGC; ATCTTCTTATCTTTCCACAGAGCTATTTACATCCTGGACTATATAACTTAAAAGAAGTAA; AACGTAATTGCACTACTGTTTTCCAGACTGGAAAAAAAAAAAAATCTCTGCAAGTGAAAC; TGTATAGAGTTTATAAAATGACTATGGATAGGGGACTGTTTTCACTTTTAGATCAAAATG; GGTTTTTAAGTAGAACCTAGGGTTTCTAATTGACTTGATTTCTGGAAATGAAAACCCGCG; CTTTTATTATGGGAAGCTTCTTGAACTGCATTTACTATTGTGAAGTTTCAAGTCCCGCTG; TAAAGATCATGTTGTTTTGTTTTCCCCAGGGCTTTCACTGTGATTTACTGCATTGCAGGC; TGTATGATAAAACACACATAATTTAAAGAGAGAAGGCTCTTGATTCCTTATGCAAGTGGA; AGAGTTGAAACTTGATTGAAGGACTTAAAACATTCACAACCTTAAGCCGAGGTGGGGGGA; TATGGGGATTCAGGCAATTGTTTACACACTTTGAATAACTGCAAAGGATTTACGGTTTGT; GAAAAATGTGTACTGTGGAAAAGATAATAAATTGAAGACATTATTGTGTGGGATTGTGCT; GATTTTTGTTGATAACACAAAAAACACTATGTTTTCTGGAGAGCTGTGTAAGCTGTCTTG; TTGCTTAGTTGCAATATAAGAAATAGTGATGTTTTGGACGTAAGTTGTCAACAAATTTCT; ATTTTATATTGTTATATTTTTATGTAGTTTGAAATGTAAAAATGTTCTAATATCAAGATT; AACAAATATAAATTTATGGTGCATTTAAAAAAAAAAAAAAAAAA;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_020860 unedited; GGGATTTGTATACGACTCCTATAGGGCGGCCGCGAAATCGGCACGAGGGGGGGATGCGCC; GCGGCGGCGGCGGCGCGGGAGCTGGGGTTGGTGTTTGGCGGCGCCAGAGCAGCGGATCCC; GGTCTCGCCGCAGCAGCAGCGCGGGTGTCGTGCACCGCCTGAAGACGCCGTACCTTTCTA; CCCCCCACCTTTTTTTTTTTTTTTTTTTAAATAACCGGAACCAATGAACGCAGCCGGGAT; CAGAGCTCCGGAGGCCGCCGGTGCCGATGGGACCAGGCTGGCGCCCGGCGGGAGCCCGTG; TCTGAGGCGGCGGGGGCGGCCGGAGGAGTCGCCGGCGGCGGTGGTGGCGCCTCGCGGAGC; CGGCGAGCTGCAGGCGGCCGGGGCGCCGCTGCGCTTTCACCCGGCTTCTCCTCGGCGCCT; TCATCCCGCCTCGACTCCTGGCCCAGCGTGGGGCTGGCTGCTGCGGCGGCGGCGCTGGGC; TGCGTTGCTGGTGCTCGGGCTGCTGGTAGCCGGAGCGGCGGACGGATGCGAGCTTGTGCC; CCGGCACCTCCGCGGGCGGCGGGCGACTGGCTCTGCCGCAACTGCCGCCTCCTCTCCCGC; CGCGGCGGCCGGCGATAGCCCGGCGCTCATGACAGATCCCTGCATGTCACTGAGTCCACC; ATGCTTTACAGAAGAAGACAGATTTAGTCTGGAAGCTCTTCAAACAATACATAAACAAAT; GGATGATGACAAAGATGGTGGAATTGAAGTAGAGGAAAGTGATGAATTCATCAGAGAAGA; TATGAAATATAAAGATGCTACTAATAAACACAGCCATCTGCACAGAGAGATAAACATATA; ACGATTGAGATTTATGGGAACGATGGAAAACATCAGAAGTCATAATTGGACCCTTGAAGA; CACTCTTCAGTGGTTGAATAGAGTTGTTGAACTACCCCAAT;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_020860 unedited; TGACGATGCACTTCCGGGCCGGAGAGCACTGGGGAGGGGTACAGGGATGCCCCCGGGATC; TGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTT; TTAAATGCACCATAAATTTATATTTGTTAATCTTGATATTAGAACATTTTTACATTTCAA; ACTACATAAAAATATAACAATATAAAATAGAAATTTGTTGACAACTTACGTCCAAAACAT; CACTATTTCTTATATTGCAACTAAGCAACAAGACAGCTTACACAGCTCTCCAGAAAACAT; AGTGTTTTTTGTGTTATCAACAAAAATCAGCACAATCCCACACAATAATGTCTTCAATTT; ATTATCTTTTCCACAGTACACATTTTTCACAAACCGTAAATCCTTTGCAGTTATTCAAAG; TGTGTAAACAATTGCCTGAATCCCCATATCCCCCCACCTCGGCTTAAGGTTGTGAATGTT; TTAAGTCCTTCAATCAAGTTTCAACTCTTCCACTTGCATAAGGAATCAAGAGCCTTCTCT; CTTTAAATTATGTGTGTTTTATCATACAGCCTGCAATGCAGTAAATCACAGTGAAAGCCC; TGGGGAAAACAAAACAACATGATCTTTACAGCGGGACTTGAAACTTCACAATAGTAAATG; CAGTTCAAGAAGCTTCCCATAATAAAAGCGCGGGTTTTCATTTCCAGAAATCAAGTCAAT; TAGAAACCCTAGGTTCTACTTAAAAACCCATTTTGATCTAAAAGTGAAAACAGTCCCCTA; TCCATAGTCATTTTATAAACTCTATACAGTTTCACTTGCAGAGATTTTTTTTTTTCCAGT; CTGAAACAGTAGTGCATTACGTTTTACTTCTTTAGTATATAGTCCAGGATGTAAATAGCT; CTGTGGGAGATAGGA;
• Restriction Sites: Please inquire
• ACCN: NM_020860
• Insert Size: 3900 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_020860.1, NP_065911.2
• RefSeq Size: 5156 bp
• RefSeq ORF: 2502 bp
• Locus ID: 57620
• UniProt ID: Q9P246
• Cytogenetics: 4p15.2
• Summary: This gene is a member of the stromal interaction molecule (STIM) family and likely arose, along with related family member STIM1, from a common ancestral gene. The encoded protein functions to regulate calcium concentrations in the cytosol and endoplasmic reticulum, and is involved in the activation of plasma membrane Orai Ca(2+) entry channels. This gene initiates translation from a non-AUG (UUG) start site. A signal peptide is cleaved from the resulting protein. Multiple transcript variants result from alternative splicing. [provided by RefSeq, Dec 2009]; Transcript Variant: This variant (2, also known as STIM2.2 or STIM2alpha) lacks an alternate in-frame exon in the central coding region, compared to variant 1, resulting in an isoform (2) that is shorter than isoform 1. This isoform functions as a positive regulator of store-operated calcium entry (SOCE).