IMPDH1 (NM_000883) Human Untagged Clone

SKU SC314151

IMPDH1 (untagged)-Human IMP (inosine 5'-monophosphate) dehydrogenase 1 (IMPDH1), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: IMPDH1
• Synonyms: IMPD; IMPD1; IMPDH-I; LCA11; RP10; sWSS2608
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_000883 edited; ATGGAGGGGCCACTCACTCCACCACCGCTGCAGGGAGGCGGAGCCGCCGCTGTTCCGGAG; CCCGGAGCCCGGCAACACCCGGGACACGAGACGGCGGCGCAGCGGTACAGCGCCCGACTG; CTGCAGGCCGGCTACGAGCCCGAGAGCCCTAGATTGGACCTCGCTACACACCCGACGACA; CCCCGTTCAGAACTATCTTCAGTGGTCTTACTGGCAGGTGTTGGTGTCCAGATGGATCGC; CTTCGCAGGGCTAGCATGGCGGACTACCTGATCAGCGGCGGCACCGGCTACGTGCCCGAG; GATGGGCTCACCGCGCAGCAGCTCTTCGCCAGCGCCGACGGCCTCACCTACAACGACTTC; CTGATTCTCCCAGGATTCATAGACTTCATAGCTGATGAGGTGGACCTGACCTCAGCCCTG; ACCCGGAAGATCACGCTGAAGACGCCACTGATCTCCTCCCCCATGGACACTGTGACAGAG; GCTGACATGGCCATTGCCATGGCTCTGATGGGAGGTATTGGTTTCATTCACCACAACTGC; ACCCCAGAGTTCCAGGCCAACGAGGTGCGGAAGGTCAAGAAGTTTGAACAGGGCTTCATC; ACGGACCCTGTGGTGCTGAGCCCCTCGCACACTGTGGGCGATGTGCTGGAGGCCAAGATG; CGGCATGGCTTCTCTGGCATCCCCATCACTGAGACGGGCACCATGGGCAGCAAGCTGGTG; GGCATCGTCACCTCCCGAGACATCGACTTTCTTGCTGAGAAGGACCACACCACCCTCCTC; AGTGAGGTGATGACGCCAAGGATTGAACTGGTGGTGGCTCCAGCAGGTGTGACGTTGAAA; GAGGCAAATGAGATCCTGCAGCGTAGCAAGAAAGGGAAGCTGCCTATCGTCAATGATTGC; GATGAGCTGGTGGCCATCATCGCCCGCACCGACCTGAAGAAGAACCGAGACTACCCTCTG; GCCTCCAAGGATTCCCAGAAGCAGCTGCTCTGTGGGGCAGCTGTGGGCACCCGTGAGGAT; GACAAATACCGTCTGGACCTGCTCACCCAGGCGGGCGTCGACGTCATAGTCTTGGACTCG; TCCCAAGGGAATTCGGTGTATCAGATCGCCATGGTGCATTACATCAAACAGAAGTACCCC; CACCTCCAGGTGATTGGGGGGAACGTGGTGACAGCAGCCCAGGCCAAGAACCTGATTGAT; GCTGGTGTGGACGGGCTGCGCGTGGGCATGGGCTGCGGCTCCATCTGCATCACCCAGGAA; GTGATGGCCTGTGGTCGGCCCCAGGGCACTGCTGTGTACAAGGTGGCTGAGTATGCCCGG; CGCTTTGGTGTGCCCATCATAGCCGATGGCGGCATCCAGACCGTGGGACACGTGGTCAAG; GCCCTGGCCCTTGGAGCCTCCACAGTGATGATGGGCTCCCTGCTGGCCGCCACTACGGAG; GCCCCTGGCGAGTACTTCTTCTCAGACGGGGTGCGGCTCAAGAAGTACCGGGGCATGGGC; TCACTGGATGCCATGGAGAAGAGCAGCAGCAGCCAGAAACGATACTTCAGCGAGGGGGAT; AAAGTGAAGATCGCRCAGGGTGTCTCGGGCTCCATCCAGGACAAAGGATCCATTCAGAAG; TTCGTGCCCTACCTCATAGCAGGCATCCAACACGGCTGCCAGGATATCGGGGCCCGCAGC; CTGTCTGTCCTTCGGTCCATGATGTACTCAGGAGAGCTCAAGTTTGAGAAGCGGACCATG; TCGGCCCAGATTGAGGGTGGTGTCCATGGCCTGCACTCTTACGAAAAGCGGCTGTACTGA;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000883 unedited; TCGTACATTCGGAGTTCGTGCCCTACCTCATAGCAGGCATCCAACACGGCTGCCAGGATA; TCGGGGCCCGCAGCCTGTCTGTCCTTCGGTCCATGATGTACTCAGGAGAGCTCAAGTTTG; AGAAGCGGACCATGTCGGCCCAGATTGAGGGTGGTGTCCATGGCCTGCACTCTTACGAAA; AGCGGCTGTACTGAGGACAGCGGTGGAGGCCGAGGTGGTGGAGGGGATGCACCCCAGTGT; CCACTTTTGGGCACAGCCTCCCTCCATAACTGAGTGGTCCACAGATTTGCACTACGGGTT; CTCCAGCTCCTTTCCAGGCAGAGAGGAGGGGAGGTCCTGAGGGGACTGCTGCCCCTCACT; CGGCATCCCCTGCAGAGTCAGGACTGCTCCCGGGGCCAGGCTGCCCTGGGAGCCCCCCTC; CGAGCCCAGCCAGCCAGGCTCTCAGGCCCTGCGCCTGCCTCAGGTCTTTCTTGCTGCAGC; CTGCTCCAGCCTGGCCCCCACCCCAGGGGCAGGCGGCCCCTCCTGGCTTCTCCTGTAGGG; CACCTCCCTGCCCCTAGCCTCCCAGGAAATGGTGCTCTCCTGGCCCTGCCTCTGGCCCTT; CCCGGGCCGCTGCCCCTCAGCCATGTGGCACTTCTGAGCTCCTGACCTAGCCAAGGGGAG; GTCTCTGCCCCCTTCCCCGGCCCTGGGCTACCCTTGGGTCCTGCTCCTCATGCCGCTCCC; CTGTCCCTGGCCATGGGTAGGAGACTGCCCTGGTCATGGCCCGCCTGCCTGTCATTCCTG; ACTCACCACCGTCCCCAGGTGAACCATTCCTCCCTTCTCCTCAGCTGCAGTCGAAGCTTT; AACTTTGCACACTTGGATCACAGTTGCGTCATTGTGTATTAAAATACTTGGAATAAATCA; ACCACGTTCTCA;
• Restriction Sites: Please inquire
• ACCN: NM_000883
• Insert Size: 2600 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_000883.2, NP_000874.2
• RefSeq Size: 2880 bp
• RefSeq ORF: 1800 bp
• Locus ID: 3614
• UniProt ID: P20839
• Cytogenetics: 7q32.1
• Domains: CBS, IMPDH
• Protein Families: Druggable Genome
• Protein Pathways: Drug metabolism - other enzymes, Metabolic pathways, Purine metabolism
• Summary: The protein encoded by this gene acts as a homotetramer to regulate cell growth. The encoded protein is an enzyme that catalyzes the synthesis of xanthine monophosphate (XMP) from inosine-5'-monophosphate (IMP). This is the rate-limiting step in the de novo synthesis of guanine nucleotides. Defects in this gene are a cause of retinitis pigmentosa type 10 (RP10). Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2008]; Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a).