B7H3 (CD276) (NM_025240) Human Untagged Clone

CAT#: SC312930

CD276 (untagged)-Human CD276 molecule (CD276), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: B7H3
• Synonyms: 4Ig-B7-H3; B7-H3; B7H3; B7RP-2
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_025240 edited
  CCGGGCCTCGCTGCGGCGGCGACTGAGCCAGGCTGGGCCGCGTCCCTGAGTCCCAGAGTC
  GGCGCGGCGCGGCAGGGGCAGCCTTCCACCACGGGGAGCCCAGCTGTCAGCCGCCTCACA
  GGAAGATGCTGCGTCGGCGGGGCAGCCCTGGCATGGGTGTGCATGTGGGTGCAGCCCTGG
  GAGCACTGTGGTTCTGCCTCACAGGAGCCCTGGAGGTCCAGGTCCCTGAAGACCCAGTGG
  TGGCACTGGTGGGCACCGATGCCACCCTGTGCTGCTCCTTCTCCCCTGAGCCTGGCTTCA
  GCCTGGCACAGCTCAACCTCATCTGGCAGCTGACAGATACCAAACAGCTGGTGCACAGCT
  TTGCTGAGGGCCAGGACCAGGGCAGCGCCTATGCCAACCGCACGGCCCTCTTCCCGGACC
  TGCTGGCACAGGGCAACGCATCCCTGAGGCTGCAGCGCGTGCGTGTGGCGGACGAGGGCA
  GCTTCACCTGCTTCGTGAGCATCCGGGATTTCGGCAGCGCTGCCGTCAGCCTGCAGGTGG
  CCGCTCCCTACTCGAAGCCCAGCATGACCCTGGAGCCCAACAAGGACCTGCGGCCAGGGG
  ACACGGTGACCATCACGTGCTCCAGCTACCGGGGCTACCCTGAGGCTGAGGTGTTCTGGC
  AGGATGGGCAGGGTGTGCCCCTGACTGGCAACGTGACCACGTCGCAGATGGCCAACGAGC
  AGGGCTTGTTTGATGTGCACAGCGTCCTGCGGGTGGTGCTGGGTGCGAATGGCACCTACA
  GCTGCCTGGTGCGCAACCCCGTGCTGCAGCAGGATGCGCACGGCTCTGTCACCATCACAG
  GGCAGCCTATGACATTCCCCCCAGAGGCCCTGTGGGTGACCGTGGGGCTGTCTGTCTGTC
  TCATTGCACTGCTGGTGGCCCTGGCTTTCGTGTGCTGGAGAAAGATCAAACAGAGCTGTG
  AGGAGGAGAATGCAGGAGCTGAGGACCAGGATGGGGAGGGAGAAGGCTCCAAGACAGCCC
  TGCAGCCTCTGAAACACTCTGACAGCAAAGAAGATGATGGACAAGAAATAGCCTGACCAT
  GAGGACCAGGGAGCTGCTACCCCTCCCTACAGCTCCTACCCTCTGGCTGCAATGGGGCTG
  CACTGTGAGCCCTGCCCCCAACAGATGCATCCTGCTCTGACAGGTGGGCTCCTTCTCCAA
  AGGATGCGATACACAGACCACTGTGCAGCCTTATTTCTCCAATGGACATGATTCCCAAGT
  CATCCTGCTGCCTTTTTTCTTATAGACACAATGAACAGACCACCCACAACCTTAGTTCTC
  TAAGTCATCCTGCCTGCTGCCTTATTTCACAGTACATACATTTCTTAGGGACACAGTACA
  CTGACCACATCACCACCCTCTTCTTCCAGTGCTGCGTGGACCATCTGGCTGCCTTTTTTC
  TCCAAAAGATGCAATATTCAGACTGACTGACCCCCTGCCTTATTTCACCAAAGACACGAT
  GCATAGTCACCCCGGCCTTGTTTCTCCAATGGCCGTGATACACTAGTGATCATGTTCAGC
  CCTGCTTCCACCTGCATAGAATCTTTTCTTCTCAGACAGGGACAGTGCGGCCTCAACATC
  TCCTGGAGTCTAGAAGCTGTTTCCTTTCCCCTCCTTCCTCCTCTTGCTCTAGCCTTAATA
  CTGGCCTTTTCCCTCCCTGCCCCAAGTGAAGACAGGGCACTCTGCGCCCACCACATGCAC
  AGCTGTGCATGGAGACCTGCAGGTGCACGTGCTGGAACACGTGTGGTTCCCCCCTGGCCC
  AGCCTCCTCTGCAGTGCCCCTCTCCCCTGCCCATCCTCCCCACGGAAGCATGTGCTGGTC
  ACACTGGTTCTCCAGGGGTCTGTGATGGGGCCCCTGGGGGTCAGCTTCTGTCCCTCTGCC
  TTCTCACCTCTTTGTTCCTTTCTTTTCATGTATCCATTCAGTTGATGTTTATTGAGCAAC
  TACAGATGTCAGCACTGTGTTAGGTGCTGGGGGCCCTGCGTGGGAAGATAAAGTTCCTCC
  CTCAAGGACTCCCCATCCAGCTGGGAGACAGACAACTAACTACACTGCACCCTGCGGTTT
  GCAGGGGGCTCCTGCCTGGCTCCCTGCTCCACACCTCCTCTGTGGCTCAAGGCTTCCTGG
  ATACCTCACCCCCATCCCACCCATAATTCTTACCCAGAGCATGGGGTTGGGGCGGAAACC
  TGGAGAGAGGGACATAGCCCCTCGCCACGGCTAGAGAATCTGGTGGTGTCCAAAATGTCT
  GTCCAGGTGTGGGCAGGTGGGCAGGCACCAAGGCCCTCTGGACCTTTCATAGCAGCAGAA
  AAGGCAGAGCCTGGGGCAGGGCAGGGCCAGGAATGCTTTGGGGACACCGAGGGGACTGCC
  CCCCACCCCCACCATGGTGCTATTCTGGGGCTGGGGCAGTCTTTTCCTGGCTTGCCTCTG
  GCCAGCTCCTGGCCTCTGGTAGAGTGAGACTTCAGACGTTCTGATGCCTTCCGGATGTCA
  TCTCTCCCTGCCCCAGGAATGGAAGATGTGAGGACTTCTAATTTAAATGTGGGACTCGGA
  GGGATTTTGTAAACTGGGGGTATATTTTGGGGAAAATAAATGTCTTTGTAAAAAGCTTAA
  AAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_025240 unedited
  ATTTCCCCCGCCCGTTGNCGCAAAGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAG
  CAGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGC
  GAATTCGGCACGAGGCCGGGCCTCGCTGCGGCGGCGACTGAGCCAGGCTGGGCCGCGTCC
  CTGAGTCCCAGAGTCGGCGCGGCGCGGCAGGGGCAGCCTTCCACCACGGGGAGCCCAGCT
  GTCAGCCGCCTCACAGGAAGATGCTGCGTCGGCGGGGCAGCCCTGGCATGGGTGTGCATG
  TGGGTGCAGCCCTGGGAGCACTGTGGTTCTGCCTCACAGGAGCCCTGGAGGTCCAGGTCC
  CTGAAGACCCAGTGGTGGCACTGGTGGGCACCGATGCCACCCTGTGCTGCTCCTTCTCCC
  CTGAGCCTGGCTTCAGCCTGGCACAGCTCAACCTCATCTGGCAGCTGACAGATACCAAAC
  AGCTGGTGCACAGCTTTGCTGAGGGCCAGGACCAGGGCAGCGCCTATGCCAACCGCACGG
  CCCTCTTCCCGGACCTGCTGGCACAGGGCAACGCATCCCTGAGGCTGCAGCGCGTGCGTG
  TGGCGGACGAGGGCAGCTTCACCTGCTTCGTGAGCATCCGGGATTTCGGCAGCGCTGCCG
  TCAGCCTGCAGGTGGCCGCTCCCTACTCGAAGCCCAGCATGACCCTGGAGCCCACAAGGA
  CCTGCGGCCAGGGGACACGGTGACCATCACGTGCTCCAGCTACCAGNGCTACCCTGAGGC
  TGAGGTGTTCTGGCAGGATGGGCAGGGTGTGCCCCTGACTGGCAACGTGACCCCGTCGCA
  GATGGCCACGAGCNAGGCTTTGTTGATGTGCACAGCATCCTGCGGGTGGTGCTGGGTGCA
  ATGGGACCTACAGCTGNCTGGGCGCAACCCCGTGCTGNACAGGNATGCCCCAGCTCTGTA
  CCATCCACCCCAGAGAG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_025240 unedited
  GNTTTATTATATTCTTTTTTTNNTTTTTTTTTTTTTNTTTTTTTTTTNTTTTTTTTTTTT
  TTTTTTTTTTTTTTTTTTTTTTTTTTTTACAAAAACATTTTTTTTTCCCCAAAATTTACC
  CCCCGTTTAAAAAAACCCCCCGGGGCCCCCATTTAAATTAAAAGGCCCCACATTTTTCCT
  TCCGGGGGGGGGGGGAAATTAACTCCGGGGGGGGTCAAAACGGGGGGAGTTTCCCTCTCC
  CAAAGGCCCGGAGCTGGGCCGAGGGAACCCCGGAAAAAACTGGCCCCCCCCCCAAATAAC
  CCCCGGGGGGGGGGGGGGGGGGGTCCCCTCGGGGGGCCCAAAAAATTTCTGGGCCTGGCC
  TGGCCCAGGGTTTGGCTTTTTTGGGGGTTTAAAAGGGCCAAAGGGGCTTGGGGGCTGGCC
  CCCTGGCCACACCTGGGGAGAAATTTTGGGCACCCCCAAATTTTTTTACCGGGGGGGGGG
  GGTTTGTCCCCCTTTCCAGGGTTCCGCCCCAACCCCCTGGTTTGGGGAAAAAATATGGGG
  GGGGGGGGGGGGGAGGGATCCAGGAAGCCCTGGGCCCCAAAAGAGGGGTGGGGCAGGGAG
  CCAAGGAGGAGCCCCCTGGAAACCCCAAGGGGGCAGGGTATTTAGTTGGCTGGCTCCCCC
  CTGGTGGGGGAGCCTTGGGGGGGGAAATTTTTTTTTCCACGCGGGGGCCCCCGGACCTAA
  AAAAGGGGGGGAGCTTGGGGGTGGCGTCAAAAAACTCCACGCGAGGGGGTCCCGGAAAAA
  AAAGGGACAAAAAGGGGAGAAGGGGGAAGGGCCAAAATCGACCCCCGGGGGGCCCTTACA
  AAACCCTGGGAAAACCGGGGGGACCCACCATGGTTCCGGGGGGGGAGGGGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_025240
• Insert Size: 2700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_025240.2, NP_079516.1
• RefSeq Size: 2765 bp
• RefSeq ORF: 951 bp
• Locus ID: 80381
• UniProt ID: Q5ZPR3
• Cytogenetics: 15q24.1
• Domains: ig, IGc2, IG
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Cell adhesion molecules (CAMs)
• Gene Summary: The protein encoded by this gene belongs to the immunoglobulin superfamily, and thought to participate in the regulation of T-cell-mediated immune response. Studies show that while the transcript of this gene is ubiquitously expressed in normal tissues and solid tumors, the protein is preferentially expressed only in tumor tissues. Additionally, it was observed that the 3' UTR of this transcript contains a target site for miR29 microRNA, and there is an inverse correlation between the expression of this protein and miR29 levels, suggesting regulation of expression of this gene product by miR29. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]
  Transcript Variant: This variant (2) lacks two alternate in-frame exons in its 5' coding region compared to variant 1. The encoded isoform (b) has the same N- and C-termini but is shorter compared to isoform a. Both variants 2 and 3 encode the same isoform (b).