PIF1 (NM_025049) Human Untagged Clone

SKU SC312706

PIF1 (untagged)-Human PIF1 5'-to-3' DNA helicase homolog (S. cerevisiae) (PIF1)

Product Data

• Type: Human Untagged Clone
• Target Symbol: PIF1
• Synonyms: C15orf20; PIF
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_025049 edited; TGAGTGTCGGCACAAACCAGCAGAGGCGGTGACGATGCTCTCGGGCATAGAGGCGGCGGC; AGGGGAATATGAGGACTCGGAGCTGCGGTGCCGCGTGGCTGTGGAGGAGCTGAGCCCGGG; CGGGCAGCCGCGAAGGCGCCAGGCCCTGCGCACCGCGGAGCTGAGCCTGGGTCGCAACGA; GCGCCGCGAGTTGATGCTGCGGCTGCAAGCGCCAGGGCCCGCGGGGCGGCCGCGCTGCTT; CCCTCTGCGCGCCGCGCGCCTCTTCACGCGTTTCGCCGAGGCCGGGCGCAGCACCCTGCG; GCTCCCCGCCCACGACACCCCCGGGGCCGGCGCAGTGCAGCTGCTGCTCTCGGACTGCCC; CCCAGACCGCCTGCGCCGCTTCCTGCGCACATTGCGCCTCAAGCTGGCTGCGGCCCCGGG; TCCCGGGCCGGCCTCCGCCCGAGCGCAGCTGCTGGGCCCGCGGCCCCGCGACTTCGTCAC; CATCAGCCCTGTGCAGCCCGAGGAGCGGCGGCTCAGGGCGGCCACCCGGGTTCCGGACAC; TACGCTGGTGAAGCGGCCTGTGGAGCCCCAGGCTGGGGCCGAGCCTAGCACAGAAGCCCC; AAGGTGGCCCCTGCCTGTGAAGAGGCTGAGCTTGCCCTCCACCAAGCCACAGCTTTCTGA; GGAACAGGCTGCTGTGCTGAGGGCCGTCCTGAAAGGCCAGAGCATCTTCTTCACTGGGAG; TGCAGGAACAGGGAAGTCATATCTGCTAAAGCGAATCCTGGGCTCACTGCCCCCCACAGG; CACTGTGGCCACTGCCAGCACTGGGGTGGCAGCCTGCCACATCGGGGGCACCACCCTCCA; TGCCTTTGCAGGCATCGGCTCAGGCCAGGCTCCTCTAGCCCAGTGTGTGGCCCTGGCCCA; AAGGCCAGGCGTGCGGCAGGGCTGGCTGAACTGCCAGCGGTTGGTCATTGACGAGATCTC; AATGGTGGAGGCAGACCTGTTTGACAAACTGGAGGCCGTGGCCAGAGCTGTCCGGCAGCA; GAACAAGCCATTCGGAGGGATCCAGCTCATCATCTGTGGGGACTTTCTGCAGCTGCCACC; TGTGACCAAGGGCTCCCAGCCCCCACGGTTCTGCTTCCAGTCCAAGAGCTGGAAGAGGTG; TGTGCCAGTGACCCTGGAGCTGACCAAGGTGTGGAGGCAGGCAGACCAGACCTTCATCTC; TCTACTGCAGGCCGTGAGGCTAGGCAGGTGTTCAGATGAGGTGACCCGCCAGCTCCAGGC; CACAGCTTCCCACAAGGTGGGGCGAGATGGGATTGTGGCCACGAGGCTCTGCACCCACCA; GGATGATGTGGCCCTCACCAACGAGAGGCGGCTTCAGGAGCTGCCAGGTAAGGTACACAG; ATTTGAGGCTATGGACAGCAACCCTGAGCTGGCCAGTACCCTGGATGCCCAGTGTCCTGT; TAGCCAGCTCCTTCAACTAAAGCTGGGGGCCCAGGTGATGCTGGTGAAAAACTTATCGGT; GTCTCGGGGCCTGGTGAATGGTGCCCGAGGGGTGGTAGTTGGGTTCGAGGCAGAAGGGAG; AGGGCTACCCCAGGTGCGGTTCCTGTGTGGAGTCACTGAGGTCATCCACGCTGACCGCTG; GACGGTGCAGGCCACCGGGGGCCAGCTCCTCAGTCGGCAGCAGCTGCCCCTCCAGCTGGC; CTGGGCGATGTCCATCCACAAGAGCCAAGGCATGACCCTGGATTGTGTGGAGATTTCTCT; GGGCCGTGTGTTTGCCAGTGGCCAGGCCTATGTGGCCCTTTCTCGGGCCCGCAGCCTGCA; GGGCCTACGTGTGCTGGACTTTGACCCCATGGCGGTTCGCTGTGACCCCCGTGTGCTGCA; CTTCTATGCCACCCTGCGGCGGGGCAGGAGCCTCAGTCTGGAGTCCCCAGATGATGATGA; GGCAGCCTCAGACCAGGAGAACATGGACCCAATCCTCTGAGCCTCACCCACAAAGAGAAG; ACAAAGGGTGGCCTGTGGCCTCCCCGTCCTCCTGCTCCCTAGTGGCCCAGGGCCTCAGGG; AATAACTGGAGTAGGCAGGCAGTGTCCCCTTCTGTATTTTTTAGGGACTCTCAGCCTCCT; GGCAGGGTTAAAGGGAGAGTACTTTAAACCCATATACCAGCTGTGCTTCAGTTTCTCTCA; CTTTGCCCTGGGTAAGCTGCTGTAGGGTCAGAAGTAACCCTTTCTGTGCCAGTTGAGAAT; GAGCCTGTGTGGTAGCTGATGTCAGAGGACAAAGCTCTCTGCAAGGGCTGGACACAGAGC; TGCAGAGTCCTGAACATCCCTCCTTTCAGGCTGCAGAAGGGAGAGGCAATGAAGACAGGT; GCTCCGGAAGCAGCATCAGGGCTCTTGGAGGGGACTGGTGGGGACTCAGGCTGGGTGCAG; CCTCCAAACAGAGAACGGAACTTAGGTGTGTCTCTACAGCTAGGCCCAGCCTAGCCCAGC; CCAGAACAAACACCCTTCAGAGCCTAACCAAAGAACATAAGCTGCAAAATGTGCACCCAT; ATTTTAAGCTGCTTTTTCAGGGGATAAATAGTGTTTGGGGACATTGAAATGGATGTTCTC; AGGTTGTATTTATTTCGGACAAATAAACTAGAGAATTGTGTAAAAAAAAAAAAAAAAAAA; AAA;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_025049 unedited; CCTCATTCCCCCGCCCGTTGNCGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATAT; AAGCAGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGC; CGCGAATTCGGCACGAGGTGAGTGTCGGCACAAACCAGCAGAGGCGGTGACGATGCTCTC; GGGCATAGAGGCGGCGGCAGGGGAATATGAGGACTCGGAGCTGCGGTGCCGCGTGGCTGT; GGAGGAGCTGAGCCCGGGCGGGCAGCCGCGAAGGCGCCAGGCCCTGCGCACCGCGGAGCT; GAGCCTGGGTCGCAACGAGCGCCGCGAGTTGATGCTGCGGCTGCAAGCGCCAGGGCCCGC; GGGGCGGCCGCGCTGCTTCCCTCTGCGCGCCGCGCGCCTCTTCACGCGTTTCGCCGAGGC; CGGGCGCAGCACCCTGCGGCTCCCCGCCCACGACACCCCCGGGGCCGGCGCAGTGCAGCT; GCTGCTCTCGGACTGCCCCCCAGACCGCCTGCGCCGCTTCCTGCGCACATTGCGCCTCAA; GCTGGCTGCGGCCCCGGGTCCCGGGCCGGCCTCCGCCCGAGCGCAGCTGCTGGGCCCGCG; GCCCCGCGACTTCGTCACCATCAGCCCTGTGCAGCCCGAGGAGCGGCGGCTCAGGGCGGC; CACCCGGGTTCCGGACACTACGCTGGTGAAGCGGCCTGTGGAGCCCCACGCTGGGGCCGC; GCCTAGCACAGAAGCCCTAGGTGGCCCCTGCCTGTGAAGAGGCTGCACTTGCCCTCCACC; AGCCCAGTTTCTGAGCACAGGCTGTGGTCTGGGTCCTCCTGACAGGCCATACATTTCTTC; ATGGTCTGACGAACCGGGAATCCTATTTGTTCAAGCGATCCTGGTCTCCTGTCCCCCCGG; CCTG;
• Restriction Sites: NotI-NotI
• ACCN: NM_025049
• Insert Size: 2600 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.; ; The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_025049.1, NP_079325.1
• RefSeq Size: 1668 bp
• RefSeq ORF: 801 bp
• Locus ID: 80119
• UniProt ID: Q9H611
• Cytogenetics: 15q22.31
• Summary: This gene encodes a DNA-dependent adenosine triphosphate (ATP)-metabolizing enzyme that functions as a 5' to 3' DNA helicase. The encoded protein can resolve G-quadruplex structures and RNA-DNA hybrids at the ends of chromosomes. It also prevents telomere elongation by inhibiting the actions of telomerase. Alternative splicing and the use of alternative start codons results in multiple isoforms that are differentially localized to either the mitochondria or the nucleus. [provided by RefSeq, Nov 2013]; Transcript Variant: This variant (2) uses an alternate splice site in the 5' UTR, compared to variant 1. This variant can initiate translation from both an upstream and a downstream AUG start codon. The isoform represented in this variant (a, also known as alpha or alpha-1) initiates translation from the upstream AUG. This isoform localizes to the nucleus (PMID: 17827721). Variants 1 and 2 encode the same isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.