AJAP1 (NM_001042478) Human Untagged Clone

CAT#: SC311268

AJAP1 (untagged)-Human adherens junctions associated protein 1 (AJAP1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: AJAP1
• Synonyms: MOT8; SHREW-1; SHREW1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC311268 representing NM_001042478.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGTGGATTCAACAGCTTTTAGGACTCAGCTCCATGTCCATCCGCTGGCCGGGCCGCCCCCTCGGAAGC
  CATGCCTGGATACTGATAGCCATGTTTCAGCTCGCCGTGGACCTGCCCGCCTGTGAGGCCCTGGGCCCG
  GGGCCGGAGTTCTGGCTCCTGCCGCGGTCGCCGCCCCGGCCGCCCCGGCTGTGGAGTTTTAGGAGTGGA
  CAGCCAGCGCGGGTCCCGGCCCCGGTGTGGAGCCCCCGGCCGCCCCGAGTGGAGCGGATCCACGGGCAG
  ATGCAGATGCCTCGAGCCAGACGGGCCCACAGGCCCCGGGACCAGGCGGCCGCCCTCGTGCCCAAGGCA
  GGACTGGCCAAGCCCCCAGCTGCTGCCAAATCCAGCCCTTCCCTCGCCTCTTCGTCCTCGTCCTCGTCC
  TCCGCGGTGGCCGGTGGGGCCCCGGAGCAGCAGGCCCTCCTGAGGAGGGGCAAGAGGCACCTGCAGGGG
  GACGGTCTCAGCAGCTTCGACTCCAGAGGCAGCCGGCCCACCACAGAGACTGAGTTCATCGCCTGGGGG
  CCCACGGGGGACGAGGAGGCCCTGGAGTCCAACACATTTCCGGGCGTTTACGGCCCCACCACGGTCTCC
  ATCCTACAAACACGGAAGACAACTGTGGCCGCCACCACCACCACCACCACCACGGCCACCCCCATGACG
  CTGCAGACTAAGGGGTTCACCGAGTCCTTGGATCCCCGGAGAAGGATCCCAGGTGGGGTTAGCACAACG
  GAGCCTTCCACCAGTCCCAGCAACAACGGGGAAGTCACCCAGCCCCCAAGGATTCTGGGGGAGGCCTCA
  GGTCTGGCTGTCCATCAGATCATCACCATCACCGTCTCCCTCATCATGGTCATAGCTGCTCTCATCACA
  ACTCTTGTCTTAAAAAATTGCTGTGCCCAAAGCGGGAACACTCGTCGGAACAGCCACCAGCGGAAGACC
  AACCAGCAGGAGGAGAGCTGCCAGAACCTCACGGACTTCCCCTCGGCCCGGGTGCCCAGCAGCCTGGAC
  ATATTCACGGCCTATAACGAGACCCTGCAGTGTTCTCACGAGTGCGTCAGGGCATCTGTGCCCGTGTAC
  ACCGATGAGACGCTGCACTCGACGACGGGGGAGTACAAATCCACATTTAATGGAAACCGACCCTCCTCT
  TCTGATCGGCATCTTATTCCTGTGGCCTTCGTGTCTGAGAAATGGTTTGAAATCTCCTGCTGA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001042478
• Insert Size: 1236 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001042478.1
• RefSeq Size: 2070 bp
• RefSeq ORF: 1236 bp
• Locus ID: 55966
• UniProt ID: Q9UKB5
• Cytogenetics: 1p36.32
• Protein Families: Druggable Genome, Transmembrane
• MW: 44.5 kDa
• Gene Summary: Plays a role in cell adhesion and cell migration.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) differs in the 3' UTR compared to variant 1. Both variants 1 and 2 encode the same protein. CCDS Note: This CCDS ID represents the protein described in PMIDs: 14595118 and 17267690. This protein is encoded by two different splice variants that are supported by AF175409.1 and AL049673.1. It should be noted that this transcript is predicted to undergo nonsense-mediated mRNA decay (NMD). However, the protein is represented because it was detected endogenously in PMID: 14595118. It is likely that the majority of transcripts representing this variant will undergo NMD, while some low level of NMD escape may allow for the expression of this protein.