Osteopontin (SPP1) (NM_001040058) Human Untagged Clone

CAT#: SC311077

SPP1 (untagged)-Human secreted phosphoprotein 1 (SPP1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Osteopontin
• Synonyms: BNSP; BSPI; ETA-1; OPN
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001040058 edited
  GAATTCGGCACGAGGCAAACGCCGACCAAGGAAAACTCACTACCATGAGAATTGCAGTGA
  TTTGCTTTTGCCTCCTAGGCATCACCTGTGCCATACCAGTTAAACAGGCTGATTCTGGAA
  GTTCTGAGGAAAAGCAGCTTTACAACAAATACCCAGATGCTGTGGCCACATGGCTAAACC
  CTGACCCATCTCAGAAGCAGAATCTCCTAGCCCCACAGAATGCTGTGTCCTCTGAAGAAA
  CCAATGACTTTAAACAAGAGACCCTTCCAAGTAAGTCCAACGAAAGCCATGACCACATGG
  ATGATATGGATGATGAAGATGATGATGACCATGTGGACAGCCAGGACTCCATTGACTCGA
  ACGACTCTGATGATGTAGATGACACTGATGATTCTCACCAGTCTGATGAGTCTCACCATT
  CTGATGAATCTGATGAACTGGTCACTGATTTTCCCACGGACCTGCCAGCAACCGAAGTTT
  TCACTCCAGTTGTCCCCACAGTAGACACATATGATGGCCGAGGTGATAGTGTGGTTTATG
  GACTGAGGTCAAAATCTAAGAAGTTTCGCAGACCTGACATCCAGTACCCTGATGCTACAG
  ACGAGGACATCACCTCACACATGGAAAGCGAGGAGTTGAATGGTGCATACAAGGCCATCC
  CCGTTGCCCAGGACCTGAACGCGCCTTCTGATTGGGACAGCCGTGGGAAGGACAGTTATG
  AAACGAGTCAGCTGGATGACCAGAGTGCTGAAACCCACAGCCACAAGCAGTCCAGATTAT
  ATAAGCGGAAAGCCAATGATGAGAGCAATGAGCATTCCGATGTGATTGATAGTCAGGAAC
  TTTCCAAAGTCAGCCGTGAATTCCACAGCCATGAATTTCACAGCCATGAAGATATGCTGG
  TTGTAGACCCCAAAAGTAAGGAAGAAGATAAACACCTGAAATTTCGTATTTCTCATGAAT
  TAGATAGTGCATCTTCTGAGGTCAATTAAAAGGAGAAAAAATACAATTTCTCACTTTGCA
  TTTAGTCAAAAGAAAAAATGCTTTATAGCAAAATGAAAGAGAACATGAAATGCTTCTTTC
  TCAGTTTATTGGTTGAATGTGTATCTATTTGAGTCTGGAAATAACTAATGTGTTTGATAA
  TTAGTTTAGTTTGTGGCTTCATGGAAACTCCCTGTAAACTAAAAGCTTCAGGGTTATGTC
  TATGTTCATTCTATAGAAGAAATGCAAACTATCACTGTATTTTAATATTTGTTATTCTCT
  CATGAATAGAAATTTATGTAGAAGCAAACAAAATACTTTTACCCACTTAAAAAGAGAATA
  TAACATTTTATGTCACTATAATCTTTTGTTTTTTAAGTTAGTGTATATTTTGTTGTGATT
  ATCTTTTTGTGGTGTGAATAAATCTTTTATCTTGAATGTAAAAAAAAAAAAAAAAAACTC
  GAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001040058 unedited
  TACGACTCACTATAGGCGGCCGCGAATTCGGCACGAGGGGCAAACGCCGACCAAGGGAAA
  ACTCACTACCATGAGAATTGCAGTGATTTGCTTTTGCCTCCTAGGCATCACCTGTGCCAT
  ACCAGTTAAACAGGCTGATTCTGGAAGTTCTGAGGAAAAGCAGCTTTACAACAAATACCC
  AGATGCTGTGGCCACATGGCTAAACCCTGACCCATCTCAGAAGCAGAATCTCCTAGCCCC
  ACAGAATGCTGTGTCCTCTGAAGAAACCAATGACTTTAAACAAGAGACCCTTCCAAGTAA
  GTCCAACGAAAGCCATGACCACATGGATGATATGGATGATGAAGATGATGATGACCATGT
  GGACAGCCAGGACTCCATTGACTCGAACGACTCTGATGATGTAGATGACACTGATGATTC
  TCACCAGTCTGATGAGTCTCACCATTCTGATGAATCTGATGAACTGGTCACTGATTTTCC
  CACGGACCTGCCAGCAACCGAAGTTTTCACTCCAGTTGTCCCCACAGTAGACACATATGA
  TGGCCGAGGTGATAGTGTGGTTTATGGACTGAGGTCAAAATCTAAGAAGTTTCGCAGACC
  TGACATCCAGTACCCTGATGCTACAGACGAGGACATCACCTCACACATGGAAAGCGAGGA
  GTTGAATGGTGCTACAAGGCCATCCCCGTTGCCCAGGACCTGAACGCGCCTTCTGATTGG
  GACAGCCGTGGGAAGGACAGTTATGAAACGAGTCAGCTGGATGACCAGAGTGCTGAAACC
  CACAGNCACAAGCAGTCCAGATTATATAAGCGGAAAGCCATGATGAGAGCNATGAGCATT
  NCGATGTAATTGATATCAGGAACTTTNCAAGTTCAGCGTGATTNCNCAGNCATGAATTNA
  CAGCATGAGAATTGCTGGTTGGAACCCCAAGTAGGAGAAGGAAACACTGAAAATTCGATC
  TATGAATAAAAGGCTTTCTGGGGCATAAAGGAAAATCAATCTCCTTGGCTATCAAGAAAA
  GCTTTCAGGA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001040058 unedited
  GGCTTTAACTATGNNACCGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTAC
  ATTCAAGATAAAAGATTTATTCACACCACAAAAAGATAATCACAACAAAATATACACTAA
  CTTAAAAAACAAAAGATTATAGTGACATAAAATGTTATATTCTCTTTTTAAGTGGGTAAA
  AGTATTTTGTTTGCTTCTACATAAATTTCTATTCATGAGAGAATAACAAATATTAAAATA
  CAGTGATAGTTTGCATTTCTTCTATAGAATGAACATAGACATAACCCTGAAGCTTTTAGT
  TTACAGGGAGTTTCCATGAAGCCACAAACTAAACTAATTATCAAACACATTAGTTATTTC
  CAGACTCAAATAGATACACATTCAACCAATAAACTGAGAAAGAAGCATTTCATGTTCTCT
  TTCATTTTGCTATAAAGCATTTTTTCTTTTGACTAAATGCAAAGTGAGAAATTGTATTTT
  TTCTCCTTTTAATTGACCTCAGAAGATGCACTATCTAATTCATGAGAAATACGAAATTTC
  AGGTGTTTATCTTCTTCCTTACTTTTGGGGTCTACAACCAGCATATCTTCATGGCTGTGA
  AATTCATGGCTGTGGAATTCACGGCTGACTTTGGAAAGTTCCTGACTATCAATCACATCG
  GAATGCTCATTGCTCTCATCATTGGCTTTCCGCTTATATAATCTGGACTGCTTGTGGCTG
  TGGGTTTCAGCACTCTGGTCATCCAGCTGACTCGTTTCATAACTGTCCTTCCCACGGCTG
  TCCCAATCAGAAGGCGCGTTCAGGTCCTGGNGCACGGGGATGGCCTTGTATGCACCATTC
  AACTCCTCGCTTTCCTGTGTGAGGNGATGTCCTCGCCTGTAGCATCAGGGTACTGGATGT
  CAGGTCTGCC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001040058
• Insert Size: 1400 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001040058.1, NP_001035147.1
• RefSeq Size: 1641 bp
• RefSeq ORF: 945 bp
• Locus ID: 6696
• UniProt ID: P10451
• Cytogenetics: 4q22.1
• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: ECM-receptor interaction, Focal adhesion, Toll-like receptor signaling pathway
• Gene Summary: The protein encoded by this gene is involved in the attachment of osteoclasts to the mineralized bone matrix. The encoded protein is secreted and binds hydroxyapatite with high affinity. The osteoclast vitronectin receptor is found in the cell membrane and may be involved in the binding to this protein. This protein is also a cytokine that upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]
  Transcript Variant: This variant (1) differs in the 5' UTR and coding sequence compared to variant 5. The resulting isoform (OPN-a, as described in PubMed: 7837791) has a shorter and distinct N-terminus compared to isoform e.