Estrogen Receptor beta (ESR2) (NM_001040276) Human Untagged Clone

SKU SC310980

ESR2 (untagged)-Human estrogen receptor 2 (ER beta) (ESR2), transcript variant c

Product Data

• Type: Human Untagged Clone
• Target Symbol: Estrogen Receptor beta
• Synonyms: ER-BETA; Erb; ESR-BETA; ESRB; ESTRB; NR3A2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001040276 edited; CCTGGGAAGCCTGACAGGGCGGCGGCAGCTGGGATGCTGGAGAGGACTGGCCCCTTGAGT; TACTGAGTCCGATGAATGTGCTTGCTCTGCTGGAGGAACCGCGCTCAGGTTACAGTCATC; CCAATATGGTTCTGAAGCCATTATACTTGCCCACGAATCTTTGAGAACATTATAATGACC; TTTGTGCCTCTTCTTGCAAGGTGTTTTCTCAGCTGTTATCTCAAGACATGGATATAAAAA; ACTCACCATCTAGCCTTAATTCTCCTTCCTCCTACAACTGCAGTCAATCCATCTTACCCC; TGGAGCACGGCTCCATATACATACCTTCCTCCTATGTAGACAGCCACCATGAATATCCAG; CCATGACATTCTATAGCCCTGCTGTGATGAATTACAGCATTCCCAGCAATGTCACTAACT; TGGAAGGTGGGCCTGGTCGGCAGACCACAAGCCCAAATGTGTTGTGGCCAACACCTGGGC; ACCTTTCTCCTTTAGTGGTCCATCGCCAGTTATCACATCTGTATGCGGAACCTCAAAAGA; GTCCCTGGTGTGAAGCAAGATCGCTAGAACACACCTTACCTGTAAACAGAGAGACACTGA; AAAGGAAGGTTAGTGGGAACCGTTGCGCCAGCCCTGTTACTGGTCCAGGTTCAAAGAGGG; ATGCTCACTTCTGCGCTGTCTGCAGCGATTACGCATCGGGATATCACTATGGAGTCTGGT; CGTGTGAAGGATGTAAGGCCTTTTTTAAAAGAAGCATTCAAGGACATAATGATTATATTT; GTCCAGCTACAAATCAGTGTACAATCGATAAAAACCGGCGCAAGAGCTGCCAGGCCTGCC; GACTTCGGAAGTGTTACGAAGTGGGAATGGTGAAGTGTGGCTCCCGGAGAGAGAGATGTG; GGTACCGCCTTGTGCGGAGACAGAGAAGTGCCGACGAGCAGCTGCACTGTGCCGGCAAGG; CCAAGAGAAGTGGCGGCCACGCGCCCCGAGTGCGGGAGCTGCTGCTGGACGCCCTGAGCC; CCGAGCAGCTAGTGCTCACCCTCCTGGAGGCTGAGCCGCCCCATGTGCTGATCAGCCGCC; CCAGTGCGCCCTTCACCGAGGCCTCCATGATGATGTCCCTGACCAAGTTGGCCGACAAGG; AGTTGGTACACATGATCAGCTGGGCCAAGAAGATTCCCGGCTTTGTGGAGCTCAGCCTGT; TCGACCAAGTGCGGCTCTTGGAGAGCTGTTGGATGGAGGTGTTAATGATGGGGCTGATGT; GGCGCTCAATTGACCACCCCGGCAAGCTCATCTTTGCTCCAGATCTTGTTCTGGACAGGG; ATGAGGGGAAATGCGTAGAAGGAATTCTGGAAATCTTTGACATGCTCCTGGCAACTACTT; CAAGGTTTCGAGAGTTAAAACTCCAACACAAAGAATATCTCTGTGTCAAGGCCATGATCC; TGCTCAATTCCAGTATGTACCCTCTGGTCACAGCGACCCAGGATGCTGACAGCAGCCGGA; AGCTGGCTCACTTGCTGAACGCCGTGACCGATGCTTTGGTTTGGGTGATTGCCAAGAGCG; GCATCTCCTCCCAGCAGCAATCCATGCGCCTGGCTAACCTCCTGATGCTCCTGTCCCACG; TCAGGCATGCGAGGGCAGAAAAGGCCTCTCAAACACTCACCTCATTTGGAATGAAGATGG; AGACTCTTTTGCCTGAAGCAACGATGGAGCAGTGA;
• Restriction Sites: Please inquire
• ACCN: NM_001040276
• Insert Size: 1700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001040276.1, NP_001035366.1
• RefSeq Size: 2745 bp
• RefSeq ORF: 1488 bp
• Locus ID: 2100
• Cytogenetics: 14q23.2-q23.3
• Protein Families: Druggable Genome, Nuclear Hormone Receptor, Transcription Factors
• Summary: This gene encodes a member of the family of estrogen receptors and superfamily of nuclear receptor transcription factors. The gene product contains an N-terminal DNA binding domain and C-terminal ligand binding domain and is localized to the nucleus, cytoplasm, and mitochondria. Upon binding to 17beta-estradiol or related ligands, the encoded protein forms homo- or hetero-dimers that interact with specific DNA sequences to activate transcription. Some isoforms dominantly inhibit the activity of other estrogen receptor family members. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been fully characterized. [provided by RefSeq, Jul 2008]; Transcript Variant: This variant (c) contains a distinct 5' UTR and uses an alternate in-frame splice site in the 3' coding region, compared to variant a. The resulting protein has a distinct C-terminus (isoform 2), compared to isoform 1, and dominantly inhibits the activity of other estrogen receptor family members. Variants b and c encode the same isoform (2).