JHDM1D (KDM7A) (NM_030647) Human Untagged Clone

SKU SC310699

JHDM1D (untagged)-Human jumonji C domain containing histone demethylase 1 homolog D (S. cerevisiae) (JHDM1D)

Product Data

• Type: Human Untagged Clone
• Target Symbol: JHDM1D
• Synonyms: JHDM1D
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_030647 edited; ATGGCCGGAGCGGCGGCGGCGGTGGCCGCGGGAGCAGCAGCTGGAGCCGCCGCGGCAGCC; GTGTCGGTGGCGGCTCCCGGCCGGGCCTCGGCGCCTCCGCCGCCCCCGCCCGTGTACTGT; GTGTGCCGGCAGCCGTACGACGTGAACCGCTTCATGATCGAGTGCGATATCTGCAAGGAC; TGGTTCCACGGCAGCTGTGTTGGAGTAGAAGAACATCATGCTGTTGACATTGACCTGTAT; CACTGTCCCAACTGTGCAGTTTTACATGGTTCCTCCTTGATGAAAAAAAGGAGGAACTGG; CACAGACATGACTACACAGAAATTGATGATGGTTCCAAACCAGTGCAAGCTGGAACTAGA; ACTTTCATTAAGGAATTACGCTCTCGAGTCTTCCCAAGTGCCGATGAAATAATTATAAAG; ATGCATGGCAGCCAGCTGACACAAAGATATCTGGAGAAACATGGATTTGATGTCCCTATT; ATGGTCCCAAAATTAGATGATCTAGGACTCAGGCTCCCTTCACCTACATTTTCTGTGATG; GATGTGGAACGTTATGTAGGTGGTGACAAAGTGATAGATGTCATTGATGTGGCGAGGCAG; GCAGACAGCAAAATGACACTTCACAATTATGTTAAATACTTCATGAATCCTAACAGACCA; AAAGTGTTAAATGTGATCAGCCTTGAATTTTCAGATACAAAGATGTCTGAATTGGTGGAG; GTCCCTGATATAGCCAAAAAACTTTCCTGGGTGGAAAATTATTGGCCAGATGATTCAGTC; TTTCCCAAGCCATTTGTTCAGAAATATTGCTTAATGGGAGTTCAAGACAGCTATACAGAT; TTCCACATTGACTTCGGTGGAACTTCAGTCTGGTACCATGTCCTCTGGGGTGAGAAGATT; TTTTATTTAATAAAGCCAACAGATGAAAATTTGGCACGTTATGAATCTTGGAGTTCATCT; GTGACCCAGAGTGAGGTGTTCTTTGGAGATAAGGTGGATAAATGCTACAAATGTGTGGTA; AAGCAGGGACATACCTTATTTGTTCCTACAGGGTGGATCCATGCTGTGCTCACTTCTCAG; GACTGTATGGCTTTTGGGGGGAACTTCCTGCACAACCTTAACATTGGCATGCAGCTCAGG; TGTTATGAGATGGAGAAAAGGCTAAAAACACCAGATCTTTTCAAATTCCCTTTCTTTGAA; GCCATATGTTGGTTTGTAGCCAAAAACTTGCTGGAAACCCTGAAAGAACTGAGAGAAGAT; GGTTTCCAGCCTCAAACTTACCTAGTACAGGGAGTGAAAGCACTGCATACTGCTTTAAAA; TTATGGATGAAAAAAGAACTTGTATCTGAACATGCCTTTGAAATTCCAGACAATGTTAGA; CCTGGACACCTTATTAAAGAACTTTCTAAAGTAATTCGAGCAATAGAGGAGGAAAACGGC; AAACCAGTTAAATCTCAGGGAATTCCTATTGTGTGTCCAGTTTCACGATCCTCAAATGAA; GCAACTTCCCCATACCATTCCCGAAGAAAGATGAGGAAACTTCGAGATCATAATGTCCGA; ACTCCTTCTAACCTAGACATCCTAGAGCTCCACACAAGGGAGGTCCTCAAAAGATTAGAG; ATGTGTCCATGGGAAGAGGACATCTTGAGCTCTAAACTGAATGGAAAATTCAACAAACAT; CTCCAACCATCCTCCACAGTACCTGAATGGAGAGCGAAAGATAATGATCTACGATTACTG; CTGACAAATGGAAGAATAATTAAAGATGAAAGGCAGCCCTTTGCAGATCAAAGTCTTTAT; ACAGCAGATAGTGAAAATGAAGAGGATAAAAGAAGGACAAAAAAGGCAAAAATGAAGATA; GAAGAGAGTTCAGGAGTAGAGGGAGTGGAACATGAAGAATCTCAAAAACCACTGAATGGG; TTTTTTACACGTGTGAAATCAGAACTCAGGAGTAGATCATCAGGATATTCTGATATTTCT; GAGTCAGAAGACTCCGGACCCGAGTGCACTGCACTGAAAAGTATCTTTACCACTGAAGAG; TCTGAAAGTTCAGGTGATGAAAAGAAACAAGAAATAACATCCAACTTTAAGGAGGAATCT; AATGTGATGAGGAACTTCCTTCAAAAGAGCCAGAAGCCATCTAGAAGTGAAATTCCAATT; AAAAGGGAATGTCCTACCTCGACGAGCACAGAGGAAGAAGCTATTCAGGGCATGCTGTCT; ATGGCAGGGTTGCACTATTCCACGTGTTTACAAAGGCAAATACAAAGCACAGACTGCAGT; GGTGAAAGAAACTCTCTCCAGGATCCCAGCAGCTGCCATGGCAGTAACCATGAGGTTAGG; CAGTTGTATCGCTATGATAAACCAGTGGAATGTGGATACCATGTCAAGACTGAAGATCCA; GACTTGAGGACTTCCTCCTGGATTAAACAGTTTGATACTTCCAGATTTCATCCTCAGGAT; CTAAGTAGAAGCCAGAAATGCATCAGAAAGGAAGGTTCATCAGAAATTAGTCAGAGGGTA; CAAAGTAGGAATTATGTGGACAGCAGCGGCTCAAGCCTTCAGAATGGAAAGTATATGCAG; AATTCAAACCTGACTTCGGGGGCGTGCCAGATAAGTAATGGCAGTCTAAGCCCAGAAAGG; CCAGTTGGTGAAACTTCCTTCTCGGTGCCCCTTCACCCCACCAAGAGACCGGCATCAAAT; CCACCACCTATCAGCAACCAGGCAACAAAAGGTAAACGTCCAAAAAAAGGAATGGCAACA; GCCAAACAACGTCTTGGGAAGATCCTTAAGTTGAACAGAAATGGCCATGCACGTTTCTTT; GTGTGA;
• Restriction Sites: Please inquire
• ACCN: NM_030647
• Insert Size: 2800 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_030647.1, NP_085150.1
• RefSeq Size: 9178 bp
• RefSeq ORF: 2826 bp
• Locus ID: 80853
• UniProt ID: Q6ZMT4
• Cytogenetics: 7q34
• Summary: Histone demethylase required for brain development. Specifically demethylates dimethylated 'Lys-9' and 'Lys-27' (H3K9me2 and H3K27me2, respectively) of histone H3 and monomethylated histone H4 'Lys-20' residue (H4K20Me1), thereby playing a central role in histone code. Specifically binds trimethylated 'Lys-4' of histone H3 (H3K4me3), affecting histone demethylase specificity: in presence of H3K4me3, it has no demethylase activity toward H3K9me2, while it has high activity toward H3K27me2. Demethylates H3K9me2 in absence of H3K4me3. Has activity toward H4K20Me1 only when nucleosome is used as a substrate and when not histone octamer is used as substrate.[UniProtKB/Swiss-Prot Function]