GPR40 (FFAR1) (NM_005303) Human Untagged Clone

SKU SC310553

TrueClone®, FFAR1 (untagged)-Human free fatty acid receptor 1 (FFAR1)

Product Data

• Type: Human Untagged Clone
• Target Symbol: GPR40
• Synonyms: FFA1R; GPCR40; GPR40
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_005303 edited; ATGGACCTGCCCCCGCAGCTCTCCTTCGGCCTCTATGTGGCCGCCTTTGCGCTGGGCTTC; CCGCTCAACGTCCTGGCCATCCGAGGCGCGACGGCCCACGCCCGGCTCCGTCTCACCCCT; AGCCTGGTCTACGCCCTGAACCTGGGCTGCTCCGACCTGCTGCTGACAGTCTCTCTGCCC; CTGAAGGCGGTGGAGGCGCTAGCCTCCGGGGCCTGGCCTCTGCCGGCCTCGCTGTGCCCC; GTCTTCGCGGTGGCCCACTTCTTCCCACTCTATGCCGGCGGGGGCTTCCTGGCCGCCCTG; AGTGCAGGCCGCTACCTGGGAGCAGCCTTCCCCTTGGGCTACCAAGCCTTCCGGAGGCCG; TGCTATTCCTGGGGGGTGTGCGCGGCCATCTGGGCCCTCGTCCTGTGTCACCTGGGTCTG; GTCTTTGGGTTGGAGGCTCCAGGAGGCTGGCTGGACCACAGCAACACCTCCCTGGGCATC; AACACACCGGTCAACGGCTCTCCGGTCTGCCTGGAGGCCTGGGACCCGGCCTCTGCCGGC; CCGGCCCGCTTCAGCCTCTCTCTCCTGCTCTTTTTTCTGCCCTTGGCCATCACAGCCTTC; TGCTACGTGGGCTGCCTCCGGGCACTGGCCCACTCCGGCCTGACGCACAGGCGGAAGCTG; CGGGCCGCCTGGGTGGCCGGCGGGGCCCTCCTCACGCTGCTGCTCTGCGTAGGACCCTAC; AACGCCTCCAACGTGGCCAGCTTCCTGTACCCCAATCTAGGAGGCTCCTGGCGGAAGCTG; GGGCTCATCACGGGTGCCTGGAGTGTGGTGCTTAATCCGCTGGTGACCGGTTACTTGGGA; AGGGGTCCTGGCCTGAAGACAGTGTGTGCGGCAAGAACGCAAGGGGGCAAGTCCCAGAAG; TAA;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005303 unedited; GGCAGTCANAATTTGTATACGACTCACTATAGGCGGCCGCATCTAGATCGAGACTAGTTC; CAGGGCTGGAACCCGCGAGTGATCCCAGGAGCCCCTCCTCTCCGGGGCCCCAAGATCTGA; GGACAGGGAGCCAGGTTGCACACAGGAGCCGTGCAGGCCAGGACGGCGGCCCCATGGACC; TGCCCCCGCAGCTCTCCTTCGGCCTCTATGTGGCCGCCTTTGCGCTGGGCTTCCCGCTCA; ACGTCCTGGCCATCCGAGGCGCGACGGCCCACGCCCGGCTCCGTCTCACCCCTAGCCTGG; TCTACGCCCTGAACCTGGGCTGCTCCGACCTGCTGCTGACAGTCTCTCTGCCCCTGAAGG; CGGTGGAGGCGCTAGCCTCCGGGGCCTGGCCTCTGCCGGCCTCGCTGTGCCCCGTCTTCG; CGGTGGCCCACTTCTTCCCACTCTATGCCGGCGGGGGCTTCCTGGCCGCCCTGAGTGCAG; GCCGCTACCTGGGAGCAGCCTTCCCCTTGGGCTACCAAGCCTTCCGGAGGCCGTGCTATT; CCTGGGGGGGTGTGCGCGGCCATCTGGGCCCTCGTCCTGTGTCACCTGGGTCTGGTCTTT; GGGTTGGAGGCTCCAGGAGGCTGGCTGGACCACAGCAACACCTCCCTGGGCATCAACACA; CCGGTCAACGGCTCTCCGGTCTGCCTGGAGGCCTGGGACCCGGCCTCTGCCGGCCCGGCC; CGCTTCAGCCTCTCTCTCCTGCTCTTTTTTCTGCCCTTGGCCATCACAGCCTTCTGCTAC; GTGGGCTGCCTCCGGGCACTGGCCCACTCCGGCCTGACGCACAGGGCGGAAACTGCGGGC; CGNCTGGGTGGCCGGGCGGGGCCCTCCTCACGCTGCTGCTCTGCGGTAGACCTTACAAGC; CTCCAAGTGGGCCGCTTCCTGTCT;
  3' Read Nucleotide Sequence

  >Forward primer walk for NM_005303 unedited; TACATAGGCTCTCCGTNTGCCTGGNAGCCTGGGNACCGGCCTCTGCCGGCCCGGCCCGCT; TCAGCCTCTCTCTCCTGCTCTTTTTTCTGCCCTTGGCCATCACAGCCTTCTGCTACGTGG; GCTGCCTCCGGGCACTGGCCCACTCCGGCCTGACGCACAGGCGGAAGCTGCGGGCCGCCT; GGGTGGCCGGCGGGGCCCTCCTCACGCTGCTGCTCTGCGTAGGACCCTACAACGCCTCCA; ACGTGGCCAGCTTCCTGTACCCCAATCTAGGAGGCTCCTGGCGGAAGCTGGGGCTCATCA; CGGGTGCCTGGAGTGTGGTGCTTAATCCGCTGGTGACCGGTTACTTGGGAAGGGGTCCTG; GCCTGAAGACAGTGTGTGCGGCAAGAACGCAAGGGGGCAAGTCCCAGAAGTAACGCCACT; GCTCGGGGGAAGGAGCATGGGGCAGGAGGGCCCGGCTGCTTCTCCAGGCCCCTGCGGGGG; GCTGCTTCGGAGGAACTGCAGGGCAGCCTGGCCCGGAGGCCTCCCTGGAGCCACTCAAGC; AGAGAGCGGCGCCTGCTGAGGGCAGCACCCCAGTCAAGAGAGGAGCACCGAGCCAGAGCA; CGGTGGCAGGGGGAGGTAAGTTTGCCCCTGCACGTGTCGAGGAAGTTTGTCCCTTCCTCG; CCTATCTTTCTCTCCCCTCTGCACGTCCTCACCTGCCTGTCTTCCGTGGGCCGCGAGCAG; AGGCCTTGTACTTACGGGAAGGGAGGAGCAGTCTGTTTCTGACCCACNAGAACTGCCACT; CCGGGTGATGCACTTTGAGGACAGCTACTCTGGAATCAGTTCCCCAGGAGAAACATTTGA; TTTCCGAGTAGGGAGAGAC;
• Restriction Sites: Please inquire
• ACCN: NM_005303
• Insert Size: 1300 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_005303.1, NP_005294.1
• RefSeq Size: 903 bp
• RefSeq ORF: 903 bp
• Locus ID: 2864
• UniProt ID: O14842
• Cytogenetics: 19q13.12
• Domains: 7tm_1
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Summary: This gene encodes a member of the GP40 family of G protein-coupled receptors that are clustered together on chromosome 19. The encoded protein is a receptor for medium and long chain free fatty acids and may be involved in the metabolic regulation of insulin secretion. Polymorphisms in this gene may be associated with type 2 diabetes. [provided by RefSeq, Apr 2009]