TRF1 (TERF1) (NM_003218) Human Untagged Clone

SKU SC310415

TERF1 (untagged)-Human telomeric repeat binding factor (NIMA-interacting) 1 (TERF1), transcript variant 2

Product Data

• Type: Human Untagged Clone
• Target Symbol: TRF1
• Synonyms: hTRF1-AS; PIN2; t-TRF1; TRBF1; TRF; TRF1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_003218 edited; ATTTTAACATGGCGGAGGATGTTTCCTCAGCGGCCCCGAGCCCGCGGGGCTGTGCGGATG; GTAGGGATGCCGACCCTACTGAGGAGCAGATGGCAGAAACAGAGAGAAACGACGAGGAGC; AGTTCGAATGCCAGGAACTGCTCGAGTGCCAGGTGCAGGTGGGGGCCCCCGAGGAGGAGG; AGGAGGAGGAGGAGGACGCGGGCCTGGTGGCCGAGGCCGAGGCCGTGGCTGCCGGCTGGA; TGCTCGATTTCCTCTGCCTCTCTCTTTGCCGAGCTTTCCGCGACGGCCGCTCCGAGGACT; TCCGCAGGACCCGCAACAGCGCAGAGGCTATTATTCATGGACTATCCAGTCTAACAGCTT; GCCAGTTGAGAACGATATACATATGTCAGTTTTTGACAAGAATTGCAGCAGGAAAAACCC; TTGATGCACAGTTTGAAAATGATGAACGAATTACACCCTTGGAATCAGCCCTGATGATTT; GGGGTTCAATTGAAAAGGAACATGACAAACTTCATGAAGAAATACAGAATTTAATTAAAA; TTCAGGCTATAGCTGTTTGTATGGAAAATGGCAACTTTAAAGAAGCAGAAGAAGTCTTTG; AAAGAATATTTGGTGATCCAAATTCTCATATGCCTTTCAAAAGCAAATTGCTTATGATAA; TCTCTCAGAAAGATACATTTCATTCCTTTTTTCAACACTTCAGCTACAACCACATGATGG; AGAAAATTAAGAGTTATGTGAATTATGTGCTAAGTGAAAAATCATCAACCTTTCTAATGA; AGGCAGCGGCAAAAGTAGTAGAAAGCAAAAGGACAAGAACAATAACTTCTCAAGATAAAC; CTAGTGGTAATGATGTTGAAATGGAAACTGAAGCTAATTTGGATACAAGAAAAAGGTCTC; ACAAGAATCTTTTCTTATCTAAGTTGCAACATGGAACCCAGCAACAAGACCTTAATAAGA; AAGAAAGAAGAGTAGGAACTCCTCAAAGTACAAAAAAGAAAAAAGAAAGCAGAAGAGCCA; CTGAAAGCAGAATACCTGTTTCAAAGAGTCAGCCGGTAACTCCTGAAAAACATCGAGCTA; GAAAAAGACAGGCATGGCTTTGGGAAGAAGACAAGAATTTGAGATCTGGCGTGAGGAAAT; ATGGAGAGGGAAACTGGTCTAAAATACTGTTGCATTATAAATTCAACAACCGGACAAGTG; TCATGTTAAAAGACAGATGGAGGACCATGAAGAAACTAAAACTGATTTCCTCAGACAGCG; AAGACTGATTGTGTTTGTAAAAGCTTGATGAAAGGACAGTTAAGTATTTTGATCACTGCA; TTTTGTTTGAAACTTGTGTCATTGATGTAATTTAAAACTTTTGTTTAAAGCATTACAGTA; TTTTTCTGTGACCATCAATTAATGAGGGTTTGTGCTACCAGAGTTAAAGCATATGCTATC; ATTGTATTCTTTAAGAACCTTATTTTGATAAAATGTAAATTTGTTGAACCCTGCCACATT; TAGTATCCCCACCCCCAAATCCTGTTCCAATGAAAAAATTAAAACCTGATACGCAAAAAA; AAAAAAAAAAAAAAAAAAAAAAAAWR;
• Restriction Sites: Please inquire
• ACCN: NM_003218
• Insert Size: 1600 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The ORF of this clone has been fully sequenced and found to contain one SNP compared with NM_003218.2.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_003218.2, NP_003209.1
• RefSeq Size: 2626 bp
• RefSeq ORF: 1260 bp
• Locus ID: 7013
• UniProt ID: P54274
• Cytogenetics: 8q21.11
• Domains: myb_DNA-binding
• Protein Families: Transcription Factors
• Summary: This gene encodes a telomere specific protein which is a component of the telomere nucleoprotein complex. This protein is present at telomeres throughout the cell cycle and functions as an inhibitor of telomerase, acting in cis to limit the elongation of individual chromosome ends. The protein structure contains a C-terminal Myb motif, a dimerization domain near its N-terminus and an acidic N-terminus. Two transcripts of this gene are alternatively spliced products. [provided by RefSeq, Jul 2008]; Transcript Variant: This variant (2) lacks an alternate in-frame exon, compared to variant 1, resulting in a shorter protein (isoform 2), compared to isoform 1.