P2RX7 (NM_002562) Human Untagged Clone

CAT#: SC310230

P2RX7 (untagged)-Human purinergic receptor P2X, ligand-gated ion channel, 7 (P2RX7), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: P2RX7
• Synonyms: P2X7
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_002562 edited
  AGGAAGAGTAGAGCTCTGGTCCAGCTCCGCGCAGGGAGGGAGGCTGTCACCATGCCGGCC
  TGCTGCAGCTGCAGTGATGTTTTCCAGTATGAGACGAACAAAGTCACTCGGATCCAGAGC
  ATGAATTATGGCACCATTAAGTGGTTCTTCCACGTGATCATCTTTTCCTACGTTTGCTTT
  GCTCTGGTGAGTGACAAGCTGTACCAGCGGAAAGAGCCTGTCATCAGTTCTGTGCACACC
  AAGGTGAAGGGGATAGCAGAGGTGAAAGAGGAGATCGTGGAGAATGGAGTGAAGAAGTTG
  GTGCACAGTGTCTTTGACACCGCAGACTACACCTTCCCTTTGCAGGGGAACTCTTTCTTC
  GTGATGACAAACTTTCTCAAAACAGAAGGCCAAGAGCAGCGGTTGTGTCCCGAGTATCCC
  ACCCGCAGGACGCTCTGTTCCTCTGACCGAGGTTGTAAAAAGGGATGGATGGACCCGCAG
  AGCAAAGGAATTCAGACCGGAAGGTGTGTAGTGTATGAAGGGAACCAGAAGACCTGTGAA
  GTCTCTGCCTGGTGCCCCATCGAGGCAGTGGAAGAGGCCCCCCGGCCTGCTCTCTTGAAC
  AGTGCCGAAAACTTCACTGTGCTCATCAAGAACAATATCGACTTCCCCGGCCACAACTAC
  ACCACGAGAAACATCCTGCCAGGTTTAAACATCACTTGTACCTTCCACAAGACTCAGAAT
  CCACAGTGTCCCATTTTCCGACTAGGAGACATCTTCCGAGAAACAGGCGATAATTTTTCA
  GATGTGGCAATTCAGGGCGGAATAATGGGCATTGAGATCTACTGGGACTGCAACCTAGAC
  CGTTGGTTCCATCACTGCCGTCCCAAATACAGTTTCCGTCGCCTTGACGACAAGACCACC
  AACGTGTCCTTGTACCCTGGCTACAACTTCAGATACGCCAAGTACTACAAGGAAAACAAT
  GTTGAGAAACGGACTCTGATAAAAGTCTTCGGGATCCGTTTTGACATCCTGGTTTTTGGC
  ACCGGAGGAAAATTTGACATTATCCAGCTGGTTGTGTACATCGGCTCAACCCTCTCCTAC
  TTCGGTCTGGCCGCTGTGTTCATCGACTTCCTCATCGACACTTACTCCAGTAACTGCTGT
  CGCTCCCATATTTATCCCTGGTGCAAGTGCTGTCAGCCCTGTGTGGTCAACGAATACTAC
  TACAGGAAGAAGTGCGAGTCCATTGTGGAGCCAAAGCCGACATTAAAGTATGTGTCCTTT
  GTGGATGAATCCCACATTAGGATGGTGAACCAGCAGCTACTAGGGAGAAGTCTGCAAGAT
  GTCAAGGGCCAAGAAGTCCCAAGACCTGCGATGGACTTCACAGATTTGTCCAGGCTGCCC
  CTGGCCCTCCATGACACACCCCCGATTCCTGGACAACCAGAGGAGATACAGCTGCTTAGA
  AAGGAGGCGACTCCTAGATCCAGGGATAGCCCCGTCTGGTGCCAGTGTGGAAGCTGCCTC
  CCATCTCAACTCCCTGAGAGCCACAGGTGCCTGGAGGCGCTGTGCTGCCGGAAAAAGCCG
  GGGGCCTGCATCACCACCTCAGAGCTGTTCAGGAAGCTGGTCCTGTCCAGACACGTCCTG
  CAGTTCCTCCTGCTCTACCAGGAGCCCTTGCTGGCGCTGGATGTGGATTCCACCAACAGC
  CGGCTGCGGCACTGTGCCTACAGGTGCTACGCCACCTGGCGCTTCGGCTCCCAGGACATG
  GCTGACTTTGCCATCCTGCCCAGCTGCTGCCGCTGGAGGATCCGGAAAGAGTTTCCGAAG
  AGTGAAGGGCAGTACAGTGGCTTCAAGAGTCCTTACTGAAGCCAGGCACCGTGGCTCACG
  TCTGTAATCCCAGCGCTTTGGGAGGCCGAGGCAGGCAGATCACCTGAGGTCGGGAGTTGG
  AGACCCGCCTGGCTAACAAGGCGAAATCCTGTCTGTACTAAAAATACAAAAATCAGCCAG
  ACATGGTGGCATGCACCTGCAATCCCAGCTACTCGGGAGGCTGAGGCACAAGAATCACTT
  GAACCCGGGTGGCA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002562 unedited
  TCACTATAGGGCGGCCGCGAATTCGGCACGAAAGGAAGAGTAGAGCTCTGGTCCAGCTCC
  GCGCAGGGAGGGAGGCTGTCACCATGCCGGCCTGCTGCAGCTGCAGTGATGTTTTCCAGT
  ATGAGACGAACAAAGTCACTCGGATCCAGAGCATGAATTATGGCACCATTAAGTGGTTCT
  TCCACGTGATCATCTTTTCCTACGTTTGCTTTGCTCTGGTGAGTGACAAGCTGTACCAGC
  GGAAAGAGCCTGTCATCAGTTCTGTGCACACCAAGGTGAAGGGGATAGCAGAGGTGAAAG
  AGGAGATCGTGGAGAATGGAGTGAAGAAGTTGGTGCACAGTGTCTTTGACACCGCAGACT
  ACACCTTCCCTTTGCAGGGGAACTCTTTCTTCGTGATGACAAACTTTCTCAAAACAGAAG
  GCCAAGAGCAGCGGTTGTGTCCCGAGTATCCCACCCGCAGGACGCTCTGTTCCTCTGACC
  GAGGTTGTAAAAAGGGATGGATGGACCCGCAGAGCAAAGGAATTCAGACCGGAAGGTGTG
  TAGTGTATGAAGGGAACCAGAAGACCTGTGAAGTCTCTGCCTGGTGCCCCATCGAGGCAG
  TGGAAGAGGCCCCCCGGCCTGCTCTCTTGAACAGTGCCGAAAACTTCACTGTGCTCATCA
  AGAACAATATCGACTTCCCCGGCCACAACTACACCACGAGAAACATCCTGCCAGGTTTTA
  AACATCACTTGTACCTTCCACAAGACTCAGAATCCACAGTGTCCCATTTTCCGACTAGGA
  GACATCTTCCGAGAAACAGGCGATAATTTTTCAC
  
• Restriction Sites: Please inquire
• ACCN: NM_002562
• Insert Size: 2100 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: There is 2 nucleotide difference between the OriGene clone and the NCBI reference ORF. OriGene considers these to be polymorphisms and to reflect the natural differences between individuals. These result in the substituion of 2 aa.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002562.4, NP_002553.2
• RefSeq Size: 3155 bp
• RefSeq ORF: 1788 bp
• Locus ID: 5027
• UniProt ID: Q99572
• Cytogenetics: 12q24.31
• Domains: P2X_receptor
• Protein Families: Druggable Genome, Ion Channels: ATP Receptors, Transmembrane
• Protein Pathways: Calcium signaling pathway, Neuroactive ligand-receptor interaction
• Gene Summary: The product of this gene belongs to the family of purinoceptors for ATP. This receptor functions as a ligand-gated ion channel and is responsible for ATP-dependent lysis of macrophages through the formation of membrane pores permeable to large molecules. Activation of this nuclear receptor by ATP in the cytoplasm may be a mechanism by which cellular activity can be coupled to changes in gene expression. Multiple alternatively spliced variants have been identified, most of which fit nonsense-mediated decay (NMD) criteria. [provided by RefSeq, Jul 2010]
  Transcript Variant: This variant (1) encodes a functional protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.