DGKZ (NM_003646) Human Untagged Clone

CAT#: SC310043

DGKZ (untagged)-Human diacylglycerol kinase, zeta 104kDa (DGKZ), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DGKZ
• Synonyms: DAGK5; DAGK6; DGK-ZETA; hDGKzeta
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_003646 edited
  CAGCGGCCCGGCCAGCTATGCGGGGTCCTGCGGCCGCGGCTGGCGGCACTTCCTGGAGCG
  GCGGCGGCAGCGGCTTCCCGGGCACCTGGGCGTGGGGAGCGGGGGCGCGCGGCGCGGGGC
  GGGCGGAGCGAGCGCGCGCCATGGAGGTGGCGGGCGGCGCGGAGCGGGCGTGCTGAGCCC
  CGGCCGCCGGCCCGGCATGGGCGTCTCCCGCGGGCCCTCCGCCGGCCGGGGCTAGGGCCG
  GATGGAGCCGCGGGACGGTAGCCCCGAGGCCCGGAGCAGCGACTCCGAGTCGGCTTCCGC
  CTCGTCCAGCGGCTCCGAGCGCGACGCCGGTCCCGAGCCGGACAAGGCGCCGCGGCGACT
  CAACAAGCGGCGCTTCCCGGGGCTGCGGCTCTTCGGGCACAGGAAAGCCATCACCAAGTC
  GGGCCTCCAGCACCTGGCCCCCCCTCCGCCCACCCCTGGGGCCCCGTGCAGCGAGTCAGA
  GCGGCAGATCCGGAGTACAGTGGACTGGAGCGAGTCAGCGACATATGGGGAGCACATCTG
  GTTCGAGACCAACGTGTCCGGGGACTTCTGCTACGTTGGGGAGCAGTACTGTGTAGCCAG
  GATGCTGAAGTCAGTGTCTCGAAGAAAGTGCGCAGCCTGCAAGATTGTGGTGCACACGCC
  CTGCATCGAGCAGCTGGAGAAGATAAATTTCCGCTGTAAGCCGTCCTTCCGTGAATCAGG
  CTCCAGGAATGTCCGCGAGCCAACCTTTGTACGGCACCACTGGGTACACAGACGACGCCA
  GGACGGCAAGTGTCGGCACTGTGGGAAGGGATTCCAGCAGAAGTTCACCTTCCACAGCAA
  GGAGATTGTGGCCATCAGCTGCTCGTGGTGCAAGCAGGCATACCACAGCAAGGTGTCCTG
  CTTCATGCTGCAGCAGATCGAGGAGCCGTGCTCGCTGGGGGTCCACGCAGCCGTGGTCAT
  CCCGCCCACCTGGATCCTCCGCGCCCGGAGGCCCCAGAATACTCTGAAAGCAAGCAAGAA
  GAAGAAGAGGGCATCCTTCAAGAGGAAGTCCAGCAAGAAAGGGCCTGAGGAGGGCCGCTG
  GAGACCCTTCATCATCAGGCCCACCCCCTCCCCGCTCATGAAGCCCCTGCTGGTGTTTGT
  GAACCCCAAGAGTGGGGGCAACCAGGGTGCAAAGATCATCCAGTCTTTCCTCTGGTATCT
  CAATCCCCGACAAGTCTTCGACCTGAGCCAGGGAGGGCCCAAGGAGGCGCTGGAGATGTA
  CCGCAAAGTGCACAACCTGCGGATCCTGGCGTGCGGGGGCGACGGCACGGTGGGCTGGAT
  CCTCTCCACCCTGGACCAGCTACGCCTGAAGCCGCCACCCCCTGTTGCCATCCTGCCCCT
  GGGTACTGGCAACGACTTGGCCCGAACCCTCAACTGGGGTGGGGGCTACACAGATGAGCC
  TGTGTCCAAGATCCTCTCCCACGTGGAGGAGGGGAACGTGGTACAGCTGGACCGCTGGGA
  CCTCCACGCTGAGCCCAACCCCGAGGCAGGGCCTGAGGACCGAGATGAAGGCGCCACCGA
  CCGGTTGCCCCTGGATGTCTTCAACAACTACTTCAGCCTGGGCTTTGACGCCCACGTCAC
  CCTGGAGTTCCACGAGTCTCGAGAGGCCAACCCAGAGAAATTCAACAGCCGCTTTCGGAA
  TAAGATGTTCTACGCCGGGACAGCTTTCTCTGACTTCCTGATGGGCAGCTCCAAGGACCT
  GGCCAAGCACATCCGAGTGGTGTGTGATGGAATGGACTTGACTCCCAAGATCCAGGACCT
  GAAACCCCAGTGTGTTGTTTTCCTGAACATCCCCAGGTACTGTGCGGGCACCATGCCCTG
  GGGCCACCCTGGGGAGCACCACGACTTTGAGCCCCAGCGGCATGACGACGGCTACCTCGA
  GGTCATTGGCTTCACCATGACGTCGTTGGCCGCGCTGCAGGTGGGCGGACACGGCGAGCG
  GCTGACGCAGTGTCGCGAGGTGGTGCTCACCACATCCAAGGCCATCCCGGTGCAGGTGGA
  TGGCGAGCCCTGCAAGCTTGCAGCCTCACGCATCCGCATCGCCCTGCGCAACCAGGCCAC
  CATGGTGCAGAAGGCCAAGCGGCGGAGCGCCGCCCCCCTGCACAGCGACCAGCAGCCGGT
  GCCAGAGCAGTTGCGCATCCAGGTGAGTCGCGTCAGCATGCACGACTATGAGGCCCTGCA
  CTACGACAAGGAGCAGCTCAAGGAGGCCTCTGTGCCGCTGGGCACTGTGGTGGTCCCAGG
  AGACAGTGACCTAGAGCTCTGCCGTGCCCACATTGAGAGACTCCAGCAGGAGCCCGATGG
  TGCTGGAGCCAAGTCCCCGACATGCCAGAAACTGTCCCCCAAGTGGTGCTTCCTGGACGC
  CACCACTGCCAGCCGCTTCTACAGGATCGACCGAGCCCAGGAGCACCTCAACTATGTGAC
  TGAGATCGCACAGGATGAGATTTATATCCTGGACCCTGAGCTGCTGGGGGCATCGGCCCG
  GCCTGACCTCCCAACCCCCACTTCCCCTCTCCCCACCTCACCCTGCTCACCCACGCCCCG
  GTCACTGCAAGGGGATGCTGCACCCCCTCAAGGTGAAGAGCTGATTGAGGCTGCCAAGAG
  GAACGACTTCTGTAAGCTCCAGGAGCTGCACCGAGCTGGGGGCGACCTCATGCACCGAGA
  CGAGCAGAGTCGCACGCTCCTGCACCACGCAGTCAGCACTGGCAGCAAGGATGTGGTCCG
  CTACCTGCTGGACCACGCCCCCCCAGAGATCCTTGATGCGGTGGAGGAAAACGGGGAGAC
  CTGTTTGCACCAAGCAGCGGCCCTGGGCCAGCGCACCATCTGCCACTACATCGTGGAGGC
  CGGGGCCTCGCTCATGAAGACAGACCAGCAGGGCGACACTCCCCGGCAGCGGGCTGAGAA
  GGCTCAGGACACCGAGCTGGCCGCCTACCTGGAGAACCGGCAGCACTACCAGATGATCCA
  GCGGGAGGACCAGGAGACGGCTGTGTAGCGGGCCGCCCACGGGCAGCAGGAGGGACAATG
  CGGCCAGGGGACGAGCGCCTTCCTTGCCCACCTCACTGCCACATTCCAGTGGGACGGCCA
  CGGGGGGACCTAGGCCCCAGGGAAAGAGCCCCATGCCGCCCCCTAAGGAGCCGCCCAGAC
  CTAGGGCTGGACTCAGGAGCTGGGGGGGCCTCACCTGTTCCCCTGAGGACCCCGCCGGAC
  CCGGAGGCTCACAGGGAACAAGACACGGCTGGGTTGGATATGCCTTTGCCGGGGTTCTGG
  GGCAGGGCGCTCCCTGGCCGCAGCAGATGCCCTCCCAGGAGTGGAGGGGCTGGAGAGGGG
  GAGGCCTTCGGGAAGAGGCTTCCTGGGCCCCCTGGTCTTCGGCCGGGTCCCCAGCCCCCG
  CTCCTGCCCCACCCCACCTCCTCCGGGCTTCCTCCCGGAAACTCAGCGCCTGCTGCACTT
  GCCTGCCCTGCCTTGCTTGGCACCCGCTCCGGCGACCCTCCCCGCTCCCCTGTCATTTCA
  TCGCGGACTGTGCGGCCTGGGGGTGGGGGGCGGGACTCTCACGGTGACATGTTTACAGCT
  GGGTGTGACTCAGTAAAGTGGATTTTTTTTTCCCAAAAAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003646 unedited
  GGCAGTTGTGTATACGACTCCTATAGGGCGGCGCGAGATATCGGCACGAGGTAGCGGCCC
  GGCCAGCTATGCGGGGTCCTGCGGCCGCGGCTGGCGGCACTTCCTGGAGCGGCGGCGGCA
  GCGGCTTCCCGGGCACCTGGGCGTGGGGAGCGGGGGCGCGCGGCGCGGGGCGGGCGGAGC
  GAGCGCGCGCCATGGAGGTGGCGGGCGGCGCGGAGCGGGCGTGCTGAGCCCCGGCCGCCG
  GCCCGGCATGGGCGTCTCCCGCGGGCCCTCCGCCGGCCGGGGCTAGGGCCGGATGGAGCC
  GCGGGACGGTAGCCCCGAGGCCCGGAGCAGCGACTCCGAGTCGGCTTCCGCCTCGTCCAG
  CGGCTCCGAGCGCGACGCCGGTCCCGAGCCGGACAAGGCGCCGCGGCGACTCAACAAGCG
  GCGCTTCCCGGGGCTGCGGCTCTTCGGGCACAGGAAAGCCATCACCAAGTCGGGCCTCCA
  GCACCTGGCCCCCCCTCCGCCCACCCCTGGGGCCCCGTGCAGCGAGTCAGAGCGGCAGAT
  CCGGAGTACAGTGGACTGGAGCGAGTCAGCGACATATGGGGAGCACATCTGGTTCGAGAC
  CAACGTGTCCGGGGACTTCTGCTACGTTGGGGAGCAGTACTGTGTAGCCAGGATGCTGAA
  GTCAGTGTCTCGAAGAAAGTGCGCAGCCTGCAAGATTGTGGTGCACACGCCCTGCATCGA
  GCAGCTGGAGAAGATAAATTTCCGCTGTAAGCCGTCCTTCCGTGAATCAGCTCCAGGAAT
  GTCCGCGAGCCAACCTTTGTACGGCACCACTGGGTAAAACAGACGACGCCAGGACGGCAG
  TGTCGCACTGTGGGAAGGGATTTCCAGCAGAAGTTCACCTTCCACAGCAAGGAGAATTGT
  GCCATCAGCTGCTCGTGGTGCAAGCCAGCATACCACAGCAAGTGTCCTGCTTCATGCTGC
  AACAGAATCGAAGGAGCCTGGCCTCGCTGGGGGGTT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003646 unedited
  CGCCGATGCACTTCAGGGCCGGAGAGGCACTGGGGTAGAGTGGTCACAGGGTATGCCACC
  CGGGATCTGTTCAGGTAATATTTTTTTTTTTTTTGAAAAAAAAATCCACTTTACTGAGTC
  ACACCCAGCTGTAAACATGTCACCGTGAGAGTCCCGCCCCCCACCCCCAGGCCGCACAGT
  CCGCGATGAAATGACAGGGGAGCGGGGAGGGTCGCCGGAGCGGGTGCCAAGCAAGGCAGG
  GCAGGCAAGTGCAGCAGGCGCTGAGTTTCCGGGAGGAAGCCCGGAGGAGGTGGGGTGGGG
  CAGGAGCGGGGGCTGGGGACCCGGCCGAAGACCAGGGGGCCCAGGAAGCCTCTTCCCGAA
  GGCCTCCCCCTCTCCAGCCCCTCCACTCCTGGGAGGGCATCTGCTGCGGCCAGGGAGCGC
  CCTGCCCCAGAACCCCGGCAAAGGCATATCCAACCCAGCCGTGTCTTGTTCCCTGTGAGC
  CTCCGGGTCCGGCGGGGTCCTCAGGGGAACAGGTGAGGCCCCCCCAGCTCCTGAGTCCAG
  CCCTAGGTCTGGGCGGCTCCTTAGGGGGCGGCATGGGGCTCTTTCCCTGGGGCCTAGGTC
  CCCCCGTGGCCGTCCCACTGGAATGTGGCAGTGAGGTGGGCAAGGAAGGCGCTCGTCCCC
  TGGCCGCATTGTCCCTCCTGCTGCCCGTGGGCGGCCCGCTACACAGCCGTCTCCTGGTCC
  TCCCGCTGGATCATCTGGTAGTGCTGCCGGTTCTCCAGGTAGGCGGCCAGCTCGGTGTCC
  TGAGCCTTCTCAGCCCGCTGCCCGGGGAGTGTCCGCCCTGCTGGTCTGTCTTCATGAGCG
  AGGCCCCGCCCTCCACGATGTAGTGGCAGATGGTGCCGCTTGCCCAGGCCGCTGCTTGGT
  GCAAACAGTCTCCCCGTTTTCCTCACCGCATCAAGATCTTCTGGGGGGGGCCGTGGGTTC
  AG
  
• Restriction Sites: Please inquire
• ACCN: NM_003646
• Insert Size: 3700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003646.3, NP_003637.2
• RefSeq Size: 3659 bp
• RefSeq ORF: 2790 bp
• Locus ID: 8525
• UniProt ID: Q13574
• Cytogenetics: 11p11.2
• Domains: DAGKa, DAGKc, ANK, DAG_PE-bind
• Protein Families: Druggable Genome
• Protein Pathways: Glycerolipid metabolism, Glycerophospholipid metabolism, Metabolic pathways, Phosphatidylinositol signaling system
• Gene Summary: The protein encoded by this gene belongs to the eukaryotic diacylglycerol kinase family. It may attenuate protein kinase C activity by regulating diacylglycerol levels in intracellular signaling cascade and signal transduction. Alternative splicing occurs at this locus and multiple transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Nov 2010]
  Transcript Variant: This variant (2) has an alternate 5' sequence including the coding region and extra nts CAG at an alternate splice site in the CDS, compared to variant 4. The resulting isoform (2) has a shorter and distinct N-terminus and an internal additional aa, compared to isoform 4.