TRPV4 (NM_021625) Human Untagged Clone

SKU SC309684

TRPV4 (untagged)-Human transient receptor potential cation channel, subfamily V, member 4 (TRPV4), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: TRPV4
• Synonyms: BCYM3; CMT2C; HMSN2C; OTRPC4; SMAL; SPSMA; SSQTL1; TRP12; VRL2; VROAC
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_021625 edited; CGGATCTCCCGGCCGCCGGCGCCCAGCCGTCCCGGAGGCTGAGCAGTGCAGACGGGCCTG; GGGCAGGCATGGCGGATTCCAGCGAAGGCCCCCGCGCGGGGCCCGGGGAGGTGGCTGAGC; TCCCCGGGGATGAGAGTGGCACCCCAGGTGGGGAGGCTTTTCCTCTCTCCTCCCTGGCCA; ATCTGTTTGAGGGGGAGGATGGCTCCCTTTCGCCCTCACCGGCTGATGCCAGTCGCCCTG; CTGGCCCAGGCGATGGGCGACCAAATCTGCGCATGAAGTTCCAGGGCGCCTTCCGCAAGG; GGGTGCCCAACCCCATCGATCTGCTGGAGTCCACCCTATATGAGTCCTCGGTGGTGCCTG; GGCCCAAGAAAGCACCCATGGACTCACTGTTTGACTACGGCACCTATCGTCACCACTCCA; GTGACAACAAGAGGTGGAGGAAGAAGATCATAGAGAAGCAGCCGCAGAGCCCCAAAGCCC; CTGCCCCTCAGCCGCCCCCCATCCTCAAAGTCTTCAACCGGCCTATCCTCTTTGACATCG; TGTCCCGGGGCTCCACTGCTGACCTGGACGGGCTGCTCCCATTCTTGCTGACCCACAAGA; AACGCCTAACTGATGAGGAGTTTCGAGAGCCATCTACGGGGAAGACCTGCCTGCCCAAGG; CCTTGCTGAACCTGAGCAATGGCCGCAACGACACCATCCCTGTGCTGCTGGACATCGCGG; AGCGCACCGGCAACATGCGGGAGTTCATTAACTCGCCCTTCCGTGACATCTACTATCGAG; GTCAGACAGCCCTGCACATCGCCATTGAGCGTCGCTGCAAACACTACGTGGAACTTCTCG; TGGCCCAGGGAGCTGATGTCCACGCCCAGGCCCGTGGGCGCTTCTTCCAGCCCAAGGATG; AGGGGGGCTACTTCTACTTTGGGGAGCTGCCCCTGTCGCTGGCTGCCTGCACCAACCAGC; CCCACATTGTCAACTACCTGACGGAGAACCCCCACAAGAAGGCGGACATGCGGCGCCAGG; ACTCGCGAGGCAACACAGTGCTGCATGCGCTGGTGGCCATTGCTGACAACACCCGTGAGA; ACACCAAGTTTGTTACCAAGATGTACGACCTGCTGCTGCTCAAGTGTGCCCGCCTCTTCC; CCGACAGCAACCTGGAGGCCGTGCTCAACAACGACGGCCTCTCGCCCCTCATGATGGCTG; CCAAGACGGGCAAGATTGGGATCTTTCAGCACATCATCCGGCGGGAGGTGACGGATGAGG; ACACACGGCACCTGTCCCGCAAGTTCAAGGACTGGGCCTATGGGCCAGTGTATTCCTCGC; TTTATGACCTCTCCTCCCTGGACACGTGTGGGGAAGAGGCCTCCGTGCTGGAGATCCTGG; TGTACAACAGCAAGATTGAGAACCGCCACGAGATGCTGGCTGTGGAGCCCATCAATGAAC; TGCTGCGGGACAAGTGGCGCAAGTTCGGGGCCGTCTCCTTCTACATCAACGTGGTCTCCT; ACCTGTGTGCCATGGTCATCTTCACTCTCACCGCCTACTACCAGCCGCTGGAGGGCACAC; CGCCGTACCCTTACCGCACCACGGTGGACTACCTGCGGCTGGCTGGCGAGGTCATTACGC; TCTTCACTGGGGTCCTGTTCTTCTTCACCAACATCAAAGACTTGTTCATGAAGAAATGCC; CTGGAGTGAATTCTCTCTTCATTGATGGCTCCTTCCAGCTGCTCTACTTCATCTACTCTG; TCCTGGTGATCGTCTCAGCAGCCCTCTACCTGGCAGGGATCGAGGCCTACCTGGCCGTGA; TGGTCTTTGCCCTGGTCCTGGGCTGGATGAATGCCCTTTACTTCACCCGTGGGCTGAAGC; TGACGGGGACCTATAGCATCATGATCCAGAAGATTCTCTTCAAGGACCTTTTCCGATTCC; TGCTCGTCTACTTGCTCTTCATGATCGGCTACGCTTCAGCCCTGGTCTCCCTCCTGAACC; CGTGTGCCAACATGAAGGTGTGCAATGAGGACCAGACCAACTGCACAGTGCCCACTTACC; CCTCGTGCCGTGACAGCGAGACCTTCAGCACCTTCCTCCTGGACCTGTTTAAGCTGACCA; TCGGCATGGGCGACCTGGAGATGCTGAGCAGCACCAAGTACCCCGTGGTCTTCATCATCC; TGCTGGTGACCTACATCATCCTCACCTTTGTGCTGCTCCTCAACATGCTCATTGCCCTCA; TGGGCGAGACAGTGGGCCAGGTCTCCAAGGAGAGCAAGCACATCTGGAAGCTGCAGTGGG; CCACCACCATCCTGGACATTGAGCGCTCCTTCCCCGTATTCCTGAGGAAGGCCTTCCGCT; CTGGGGAGATGGTCACCGTGGGCAAGAGCTCGGACGGCACTCCTGACCGCAGGTGGTGCT; TCAGGGTGGATGAGGTGAACTGGTCTCACTGGAACCAGAACTTGGGCATCATCAACGAGG; ACCCGGGCAAGAATGAGACCTACCAGTATTATGGCTTCTCGCATACCGTGGGCCGCCTCC; GCAGGGATCGCTGGTCCTCGGTGGTACCCCGCGTGGTGGAACTGAACAAGAACTCGAACC; CGGACGAGGTGGTGGTGCCTCTGGACAGCATGGGGAACCCCCGCTGCGATGGCCACCAGC; AGGGTTACCCCCGCAAGTGGAGGACTGATGACGCCCCGCTCTAGGGACTGCAGCCCAGCC; CCAGCTTCTCTGCCCACTCATTTCTAGTCCAGCCGCATTTCAGCAGTGCCTTCTGGGGTG; TCCCCCCACACCCTGCTTTGGCCCCAGAGGCGAGGGACCAGTGGAGGTGCCAGGGAGGCC; CCAGGACCCTGTGGTCCCCTGGCTCTGCCTCCCCACCCTGGGGTGGGGGCTCCCGGCCAC; CTGTCTTGCTCCTATGGAGTCACATAAGCCAACGCCAGAGCCCCTCCACCTCAGGCCCCA; GCCCCTGCCTCTCCATTATTTATTTGCTCTGCTCTCAGGAAGCGACGTGACCCCTGCCCC; AGCTGGAACCTGGCAGAGGCCTTAGGACCCCGTTCCAAGTGCACTGCCCGGCCAAGCCCC; AGCCTCAGCCTGCGCCTGAGCTGCATGCGCCACCATTTTTGGCAGCGTGGCAGCTTTGCA; AGGGGCTGGGGCCCTCGGCGTGGGGCCATGCCTTCTGTGTGTTCTGTAGTGTCTGGGATT; TGCCGGTGCTCAATAAATGTTTATTCATTGAAAAAAAAAAAAAAAAAAAAAAA;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_021625 unedited; GTGGCTGTCAGATTTTGTATACGACTCATATAGGCGGCCGCGCAATTCGCACGAGGCGGA; TCTCCCGGCCGCCGGCGCCCAGCCGTCCCGGAGGCTGAGCAGTGCAGACGGGCCTGGGGC; AGGCATGGCGGATTCCAGCGAAGGCCCCCGCGCGGGGCCCGGGGAGGTGGCTGAGCTCCC; CGGGGATGAGAGTGGCACCCCAGGTGGGGAGGCTTTTCCTCTCTCCTCCCTGGCCAATCT; GTTTGAGGGGGAGGATGGCTCCCTTTCGCCCTCACCGGCTGATGCCAGTCGCCCTGCTGG; CCCAGGCGATGGGCGACCAAATCTGCGCATGAAGTTCCAGGGCGCCTTCCGCAAGGGGGT; GCCCAACCCCATCGATCTGCTGGAGTCCACCCTATATGAGTCCTCGGTGGTGCCTGGGCC; CAAGAAAGCACCCATGGACTCACTGTTTGACTACGGCACCTATCGTCACCACTCCAGTGA; CAACAAGAGGTGGAGGAAGAAGATCATAGAGAAGCAGCCGCAGAGCCCCAAAGCCCCTGC; CCCTCAGCCGCCCCCCATCCTCAAAGTCTTCAACCGGCCTATCCTCTTTGACATCGTGTC; CCGGGGCTCCACTGCTGACCTGGACGGGCTGCTCCCATTCTTGCTGACCCACAAGAAACG; CCTAACTGATGAGGAGTTTCGAGAGCCATCTACGGGGAAGACCTGCCTGCCCAAGGCCTT; GCTGAACCTGAGCAATGGCCGCAACGACACCATCCCTGTGCTGCTGGACATCGCGGAGCG; CACCGGCAACATGCGGGAGTTCATTAACTCGCCCTTCCGTGACATCTACTATCGAGGTCA; GACAGCCCTGCACATCGCCATTGAGCGTCCCTTGCAACACTACGTGGAACTTCTCGTGCC; CCAGGNAGCTGATGTCC;
• Restriction Sites: NotI-NotI
• ACCN: NM_021625
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_021625.3, NP_067638.3
• RefSeq Size: 3254 bp
• RefSeq ORF: 2616 bp
• Locus ID: 59341
• UniProt ID: Q9HBA0
• Cytogenetics: 12q24.11
• Domains: ANK, ion_trans
• Protein Families: Druggable Genome, Ion Channels: Transient receptor potential, Transmembrane
• Summary: This gene encodes a member of the OSM9-like transient receptor potential channel (OTRPC) subfamily in the transient receptor potential (TRP) superfamily of ion channels. The encoded protein is a Ca2+-permeable, nonselective cation channel that is thought to be involved in the regulation of systemic osmotic pressure. Mutations in this gene are the cause of spondylometaphyseal and metatropic dysplasia and hereditary motor and sensory neuropathy type IIC. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2010]; Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a).