Dicer (DICER1) (NM_177438) Human Untagged Clone

CAT#: SC309637

DICER1 (untagged)-Human dicer 1, ribonuclease type III (DICER1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Dicer
• Synonyms: DCR1; Dicer; Dicer1e; GLOW; HERNA; K12H4.8-LIKE; MNG1; RMSE2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC309637 representing NM_177438.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGAAAAGCCCTGCTTTGCAACCCCTCAGCATGGCAGGCCTGCAGCTCATGACCCCTGCTTCCTCACCA
  ATGGGTCCTTTCTTTGGACTGCCATGGCAACAAGAAGCAATTCATGATAACATTTATACGCCAAGAAAA
  TATCAGGTTGAACTGCTTGAAGCAGCTCTGGATCATAATACCATCGTCTGTTTAAACACTGGCTCAGGG
  AAGACATTTATTGCAGTACTACTCACTAAAGAGCTGTCCTATCAGATCAGGGGAGACTTCAGCAGAAAT
  GGAAAAAGGACGGTGTTCTTGGTCAACTCTGCAAACCAGGTTGCTCAACAAGTGTCAGCTGTCAGAACT
  CATTCAGATCTCAAGGTTGGGGAATACTCAAACCTAGAAGTAAATGCATCTTGGACAAAAGAGAGATGG
  AACCAAGAGTTTACTAAGCACCAGGTTCTCATTATGACTTGCTATGTCGCCTTGAATGTTTTGAAAAAT
  GGTTACTTATCACTGTCAGACATTAACCTTTTGGTGTTTGATGAGTGTCATCTTGCAATCCTAGACCAC
  CCCTATCGAGAAATTATGAAGCTCTGTGAAAATTGTCCATCATGTCCTCGCATTTTGGGACTAACTGCT
  TCCATTTTAAATGGGAAATGTGATCCAGAGGAATTGGAAGAAAAGATTCAGAAACTAGAGAAAATTCTT
  AAGAGTAATGCTGAAACTGCAACTGACCTGGTGGTCTTAGACAGGTATACTTCTCAGCCATGTGAGATT
  GTGGTGGATTGTGGACCATTTACTGACAGAAGTGGGCTTTATGAAAGACTGCTGATGGAATTAGAAGAA
  GCACTTAATTTTATCAATGATTGTAATATATCTGTACATTCAAAAGAAAGAGATTCTACTTTAATTTCG
  AAACAGATACTATCAGACTGTCGTGCCGTATTGGTAGTTCTGGGACCCTGGTGTGCAGATAAAGTAGCT
  GGAATGATGGTAAGAGAACTACAGAAATACATCAAACATGAGCAAGAGGAGCTGCACAGGAAATTTTTA
  TTGTTTACAGACACTTTCCTAAGGAAAATACATGCACTATGTGAAGAGCACTTCTCACCTGCCTCACTT
  GACCTGAAATTTGTAACTCCTAAAGTAATCAAACTGCTCGAAATCTTACGCAAATATAAACCATATGAG
  CGACAGCAGTTTGAAAGCGTTGAGTGGTATAATAATAGAAATCAGGATAATTATGTGTCATGGAGTGAT
  TCTGAGGATGATGATGAGGATGAAGAAATTGAAGAAAAAGAGAAGCCAGAGACAAATTTTCCTTCTCCT
  TTTACCAACATTTTGTGCGGAATTATTTTTGTGGAAAGAAGATACACAGCAGTTGTCTTAAACAGATTG
  ATAAAGGAAGCTGGCAAACAAGATCCAGAGCTGGCTTATATCAGTAGCAATTTCATAACTGGACATGGC
  ATTGGGAAGAATCAGCCTCGCAACAAACAGATGGAAGCAGAATTCAGAAAACAGGAAGAGGTACTTAGG
  AAATTTCGAGCACATGAGACCAACCTGCTTATTGCAACAAGTATTGTAGAAGAGGGTGTTGATATACCA
  AAATGCAACTTGGTGGTTCGTTTTGATTTGCCCACAGAATATCGATCCTATGTTCAATCTAAAGGAAGA
  GCAAGGGCACCCATCTCTAATTATATAATGTTAGCGGATACAGACAAAATAAAAAGTTTTGAAGAAGAC
  CTTAAAACCTACAAAGCTATTGAAAAGATCTTGAGAAACAAGTGTTCCAAGTCGGTTGATACTGGTGAG
  ACTGACATTGATCCTGTCATGGATGATGATGACGTTTTCCCACCATATGTGTTGAGGCCTGACGATGGT
  GGTCCACGAGTCACAATCAACACGGCCATTGGACACATCAATAGATACTGTGCTAGATTACCAAGTGAT
  CCGTTTACTCATCTAGCTCCTAAATGCAGAACCCGAGAGTTGCCTGATGGTACATTTTATTCAACTCTT
  TATCTGCCAATTAACTCACCTCTTCGAGCCTCCATTGTTGGTCCACCAATGAGCTGTGTACGATTGGCT
  GAAAGAGTTGTAGCTCTCATTTGCTGTGAGAAACTGCACAAAATTGGCGAACTGGATGACCATTTGATG
  CCAGTTGGGAAAGAGACTGTTAAATATGAAGAGGAGCTTGATTTGCATGATGAAGAAGAGACCAGTGTT
  CCAGGAAGACCAGGTTCCACGAAACGAAGGCAGTGCTACCCAAAAGCAATTCCAGAGTGTTTGAGGGAT
  AGTTATCCCAGACCTGATCAGCCCTGTTACCTGTATGTGATAGGAATGGTTTTAACTACACCTTTACCT
  GATGAACTCAACTTTAGAAGGCGGAAGCTCTATCCTCCTGAAGATACCACAAGATGCTTTGGAATACTG
  ACGGCCAAACCCATACCTCAGATTCCACACTTTCCTGTGTACACACGCTCTGGAGAGGTTACCATATCC
  ATTGAGTTGAAGAAGTCTGGTTTCATGTTGTCTCTACAAATGCTTGAGTTGATTACAAGACTTCACCAG
  TATATATTCTCACATATTCTTCGGCTTGAAAAACCTGCACTAGAATTTAAACCTACAGACGCTGATTCA
  GCATACTGTGTTCTACCTCTTAATGTTGTTAATGACTCCAGCACTTTGGATATTGACTTTAAATTCATG
  GAAGATATTGAGAAGTCTGAAGCTCGCATAGGCATTCCCAGTACAAAGTATACAAAAGAAACACCCTTT
  GTTTTTAAATTAGAAGATTACCAAGATGCCGTTATCATTCCAAGATATCGCAATTTTGATCAGCCTCAT
  CGATTTTATGTAGCTGATGTGTACACTGATCTTACCCCACTCAGTAAATTTCCTTCCCCTGAGTATGAA
  ACTTTTGCAGAATATTATAAAACAAAGTACAACCTTGACCTAACCAATCTCAACCAGCCACTGCTGGAT
  GTGGACCACACATCTTCAAGACTTAATCTTTTGACACCTCGACATTTGAATCAGAAGGGGAAAGCGCTT
  CCTTTAAGCAGTGCTGAGAAGAGGAAAGCCAAATGGGAAAGTCTGCAGAATAAACAGATACTGGTTCCA
  GAACTCTGTGCTATACATCCAATTCCAGCATCACTGTGGAGAAAAGCTGTTTGTCTCCCCAGCATACTT
  TATCGCCTTCACTGCCTTTTGACTGCAGAGGAGCTAAGAGCCCAGACTGCCAGCGATGCTGGCGTGGGA
  GTCAGATCACTTCCTGCGGATTTTAGATACCCTAACTTAGACTTCGGGTGGAAAAAATCTATTGACAGC
  AAATCTTTCATCTCAATTTCTAACTCCTCTTCAGCTGAAAATGATAATTACTGTAAGCACAGCACAATT
  GTCCCTGAAAATGCTGCACATCAAGGTGCTAATAGAACCTCCTCTCTAGAAAATCATGACCAAATGTCT
  GTGAACTGCAGAACGTTGCTCAGCGAGTCCCCTGGTAAGCTCCACGTTGAAGTTTCAGCAGATCTTACA
  GCAATTAATGGTCTTTCTTACAATCAAAATCTCGCCAATGGCAGTTATGATTTAGCTAACAGAGACTTT
  TGCCAAGGAAATCAGCTAAATTACTACAAGCAGGAAATACCCGTGCAACCAACTACCTCATATTCCATT
  CAGAATTTATACAGTTACGAGAACCAGCCCCAGCCCAGCGATGAATGTACTCTCCTGAGTAATAAATAC
  CTTGATGGAAATGCTAACAAATCTACCTCAGATGGAAGTCCTGTGATGGCCGTAATGCCTGGTACGACA
  GACACTATTCAAGTGCTCAAGGGCAGGATGGATTCTGAGCAGAGCCCTTCTATTGGGTACTCCTCAAGG
  ACTCTTGGCCCCAATCCTGGACTTATTCTTCAGGCTTTGACTCTGTCAAACGCTAGTGATGGATTTAAC
  CTGGAGCGGCTTGAAATGCTTGGCGACTCCTTTTTAAAGCATGCCATCACCACATATCTATTTTGCACT
  TACCCTGATGCGCATGAGGGCCGCCTTTCATATATGAGAAGCAAAAAGGTCAGCAACTGTAATCTGTAT
  CGCCTTGGAAAAAAGAAGGGACTACCCAGCCGCATGGTGGTGTCAATATTTGATCCCCCTGTGAATTGG
  CTTCCTCCTGGTTATGTAGTAAATCAAGACAAAAGCAACACAGATAAATGGGAAAAAGATGAAATGACA
  AAAGACTGCATGCTGGCGAATGGCAAACTGGATGAGGATTACGAGGAGGAGGATGAGGAGGAGGAGAGC
  CTGATGTGGAGGGCTCCGAAGGAAGAGGCTGACTATGAAGATGATTTCCTGGAGTATGATCAGGAACAT
  ATCAGATTTATAGATAATATGTTAATGGGGTCAGGAGCTTTTGTAAAGAAAATCTCTCTTTCTCCTTTT
  TCAACCACTGATTCTGCATATGAATGGAAAATGCCCAAAAAATCCTCCTTAGGTAGTATGCCATTTTCA
  TCAGATTTTGAGGATTTTGACTACAGCTCTTGGGATGCAATGTGCTATCTGGATCCTAGCAAAGCTGTT
  GAAGAAGATGACTTTGTGGTGGGGTTCTGGAATCCATCAGAAGAAAACTGTGGTGTTGACACGGGAAAG
  CAGTCCATTTCTTACGACTTGCACACTGAGCAGTGTATTGCTGACAAAAGCATAGCGGACTGTGTGGAA
  GCCCTGCTGGGCTGCTATTTAACCAGCTGTGGGGAGAGGGCTGCTCAGCTTTTCCTCTGTTCACTGGGG
  CTGAAGGTGCTCCCGGTAATTAAAAGGACTGATCGGGAAAAGGCCCTGTGCCCTACTCGGGAGAATTTC
  AACAGCCAACAAAAGAACCTTTCAGTGAGCTGTGCTGCTGCTTCTGTGGCCAGTTCACGCTCTTCTGTA
  TTGAAAGACTCGGAATATGGTTGTTTGAAGATTCCACCAAGATGTATGTTTGATCATCCAGATGCAGAT
  AAAACACTGAATCACCTTATATCGGGGTTTGAAAATTTTGAAAAGAAAATCAACTACAGATTCAAGAAT
  AAGGCTTACCTTCTCCAGGCTTTTACACATGCCTCCTACCACTACAATACTATCACTGATTGTTACCAG
  CGCTTAGAATTCCTGGGAGATGCGATTTTGGACTACCTCATAACCAAGCACCTTTATGAAGACCCGCGG
  CAGCACTCCCCGGGGGTCCTGACAGACCTGCGGTCTGCCCTGGTCAACAACACCATCTTTGCATCGCTG
  GCTGTAAAGTACGACTACCACAAGTACTTCAAAGCTGTCTCTCCTGAGCTCTTCCATGTCATTGATGAC
  TTTGTGCAGTTTCAGCTTGAGAAGAATGAAATGCAAGGAATGGATTCTGAGCTTAGGAGATCTGAGGAG
  GATGAAGAGAAAGAAGAGGATATTGAAGTTCCAAAGGCCATGGGGGATATTTTTGAGTCGCTTGCTGGT
  GCCATTTACATGGATAGTGGGATGTCACTGGAGACAGTCTGGCAGGTGTACTATCCCATGATGCGGCCA
  CTAATAGAAAAGTTTTCTGCAAATGTACCCCGTTCCCCTGTGCGAGAATTGCTTGAAATGGAACCAGAA
  ACTGCCAAATTTAGCCCGGCTGAGAGAACTTACGACGGGAAGGTCAGAGTCACTGTGGAAGTAGTAGGA
  AAGGGGAAATTTAAAGGTGTTGGTCGAAGTTACAGGATTGCCAAATCTGCAGCAGCAAGAAGAGCCCTC
  CGAAGCCTCAAAGCTAATCAACCTCAGGTTCCCAATAGCTGA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_177438
• Insert Size: 5769 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_177438.2
• RefSeq Size: 10323 bp
• RefSeq ORF: 5769 bp
• Locus ID: 23405
• UniProt ID: Q9UPY3
• Cytogenetics: 14q32.13
• Protein Families: Druggable Genome
• MW: 218.7 kDa
• Gene Summary: This gene encodes a protein possessing an RNA helicase motif containing a DEXH box in its amino terminus and an RNA motif in the carboxy terminus. The encoded protein functions as a ribonuclease and is required by the RNA interference and small temporal RNA (stRNA) pathways to produce the active small RNA component that represses gene expression. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2010]
  Transcript Variant: This variant (1) differs in the 5' UTR, compared to variant 2. Variants 1, 2, 4 and 5 encode the same isoform (1).