BPTF (NM_004459) Human Untagged Clone

SKU SC309497

BPTF (untagged)-Human bromodomain PHD finger transcription factor (BPTF), transcript variant 2

Product Data

• Type: Human Untagged Clone
• Target Symbol: BPTF
• Synonyms: FAC1; FALZ; NEDDFL; NURF301
• Vector: pCMV6 series
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_004459, the custom clone sequence may differ by one or more nucleotides; ATGAGGGGCCGGCGGGGCAGGCCGCCCAAGCAGCCCGCGGCTCCCGCTGCGGAGCGCTGC; GCCCCGGCCCCGCCGCCACCGCCGCCGCCGCCCACGTCCGGACCCATCGGGGGGCTCCGC; TCGCGGCACCGCGGCAGCAGCCGGGGCAGGTGGGCCGCCGCCCAGGCTGAGGTGGCGCCC; AAGACGCGGCTGAGCTCGCCCAGGGGGGGCAGCAGTAGCCGGAGGAAGCCGCCGCCGCCG; CCGCCGGCCCCCCCCAGCACCAGCGCCCCGGGCCGGGGGGGGCGAGGAGGCGGGGGCGGC; AGGACGGGGGGCGGGGGCGGCGGCGGCCACCTGGCCCGGACCACCGCGGCCCGGAGGGCC; GTCAACAAAGTGGTGTACGATGACCACGAGAGCGAGGAGGAGGAGGAAGAGGAGGACATG; GTCTCCGAGGAGGAGGAGGAGGAGGACGGCGACGCCGAGGAGACCCAGGATTCTGAGGAC; GACGAGGAGGATGAGATGGAAGAGGACGACGATGACTCCGATTATCCGGAGGAGATGGAA; GACGACGACGACGACGCCAGTTACTGCACGGAAAGCAGCTTCAGGAGCCATAGTACCTAC; AGCAGCACTCCAGGTAGGCGAAAACCAAGAGTACATCGGCCTCGTTCTCCTATATTGGAA; GAAAAAGACATCCCGCCCCTTGAATTTCCCAAGTCCTCTGAGGATTTAATGGTGCCTAAT; GAGCATATAATGAATGTCATTGCCATTTACGAGGTACTGCGGAACTTTGGCACTGTTTTG; AGATTATCTCCTTTTCGCTTTGAGGACTTTTGTGCAGCTCTGGTGAGCCAAGAGCAGTGC; ACACTCATGGCAGAGATGCATGTTGTGCTTTTGAAAGCAGTTCTGCGTGAAGAAGACACT; TCCAATACTACCTTTGGACCTGCTGATCTGAAAGATAGCGTTAATTCCACACTGTATTTC; ATAGATGGGATGACGTGGCCAGAGGTGCTGCGGGTGTACTGTGAGAGTGATAAGGAGTAC; CATCACGTTCTTCCTTACCAAGAGGCAGAGGACTACCCATATGGACCAGTAGAGAACAAG; ATCAAAGTTCTACAGTTTCTAGTCGATCAGTTTCTTACAACAAATATTGCTCGAGAGGAA; TTGATGTCTGAAGGGGTGATACAGTATGATGACCATTGTAGGGTTTGTCACAAACTTGGG; GATTTGCTTTGCTGTGAGACATGTTCAGCAGTATACCATTTGGAATGTGTGAAGCCACCT; CTTGAGGAGGTGCCAGAGGACGAGTGGCAGTGTGAAGTCTGTGTAGCACACAAGGTGCCT; GGTGTGACTGACTGTGTTGCTGAAATCCAAAAAAATAAACCATATATTCGACATGAACCT; ATTGGATATGATAGAAGTCGGAGGAAATACTGGTTCTTGAACCGAAGACTCATAATAGAA; GAAGATACAGAAAATGAAAATGAAAAGAAAATTTGGTATTACAGCACAAAGGTCCAACTT; GCAGAATTAATTGACTGTCTAGACAAAGATTATTGGGAAGCAGAACTCTGCAAAATTCTA; GAAGAAATGCGTGAAGAAATCCACCGACACATGGACATAACTGAAGACCTGACCAATAAG; GCTCGGGGCAGTAACAAATCCTTTCTGGCGGCAGCTAATGAAGAAATTTTGGAATCCATA; AGAGCCAAAAAGGGAGACATTGATAATGTTAAAAGCCCAGAAGAAACAGAAAAAGACAAG; AATGAGACTGAGAATGACTCTAAAGATGCTGAGAAAAACAGAGAAGAATTTGAAGACCAG; TCCCTTGAAAAAGACAGTGACGACAAAACACCAGATGATGACCCTGAGCAAGGAAAATCT; GAGGAGCCAACAGAAGTTGGGGATAAAGGTAACTCTGTGTCAGCAAATCTTGGCGACAAC; ACAACAAATGCAACTTCAGAAGAGACTAGTCCCTCTGAAGGGAGGAGCCCTGTGGGGTGT; CTCTCAGAAACCCCCGATAGCAGCAACATGGCAGAGAAGAAGGTGGCATCTGAGCTCCCC; CAGGATGTGCCAGAAGAACCTAACAAGACATGTGAGAGCAGTAACACTAGTGCTACCACT; ACCTCCATCCAGCCTAATCTGGAAAACAGTAACAGCAGCAGTGAACTAAATTCTTCCCAG; AGTGAATCTGCTAAGGCAGCTGATGATCCTGAAAATGGAGAAAGAGAATCTCATACACCT; GTCTCTATTCAGGAAGAGATAGTAGGTGATTTCAAATCGGAGAAGTCCAACGGGGAGCTA; AGTGAATCTCCTGGAGCTGGAAAAGGAGCATCTGGCTCAACTCGAATCATCACCAGATTG; CGGAATCCAGATAGCAAACTTAGTCAGCTGAAGAGCCAGCAGGTGGCAGCCGCTGCACAT; GAAGCAAATAAATTATTTAAGGAGGGCAAAGAGGTACTGGTAGTTAACTCTCAAGGAGAA; ATTTCACGGTTGAGCACCAAAAAGGAAGTGATCATGAAAGGAAATATCAACAATTATTTT; AAATTGGGTCAAGAAGGGAAGTATCGCGTCTACCACAATCAATACTCCACCAATTCATTT; GCTTTGAATAAGCACCAGCACAGAGAAGACCATGATAAGAGAAGGCATCTTGCACATAAG; TTCTGTCTGACTCCAGCAGGAGAGTTCAAATGGAACGGTTCTGTCCATGGGTCCAAAGTT; CTTACCATATCTACTCTGAGACTGACTATCACCCAATTAGAAAACAACATCCCTTCATCC; TTTCTTCATCCCAACTGGGCATCACATAGGGCAAATTGGATCAAGGCAGTTCAGATGTGT; AGCAAACCCAGAGAATTTGCATTGGCTTTAGCCATTTTGGAGTGTGCAGTTAAACCAGTT; GTGATGCTACCAATATGGCGAGAATCTTTAGGACATACCAGGTTACACCGGATGACATCA; ATTGAAAGAGAAGAAAAGGAGAAAGTCAAAAAAAAAGAGAAGAAACAGGAAGAAGAAGAA; ACGATGCAGCAAGCGACATGGGTAAAATACACATTTCCAGTTAAGCATCAGGTTTGGAAA; CAAAAAGGTGAAGAGTACAGAGTGACAGGATATGGTGGTTGGAGCTGGATTAGTAAAACT; CATGTTTATAGGTTTGTTCCTAAATTGCCAGGCAATACTAATGTGAATTACAGAAAGTCG; TTAGAAGGAACCAAAAATAATATGGATGAAAATATGGATGAGTCAGATAAAAGAAAATGT; TCACGAAGTCCAAAAAAAATAAAAATAGAGCCTGATTCTGAAAAAGATGAGGTAAAAGGT; TCAGATGCTGCAAAAGGAGCAGACCAAAATGAAATGGATATCTCAAAGATTACTGAGAAG; AAGGACCAAGATGTGAAGGAGCTCTTAGATTCTGACAGTGATAAACCCTGCAAGGAAGAA; CCAATGGAAGTAGACGATGACATGAAAACAGAGTCACATGTAAATTGTCAGGAGAGTTCT; CAAGTAGATGTGGTCAATGTTAGTGAGGGTTTTCATCTAAGGACTAGTTACAAAAAGAAA; ACAAAATCATCCAAACTAGATGGACTTCTTGAAAGGAGAATTAAACAGTTTACACTGGAA; GAAAAACAGCGACTCGAAAAAATCAAGTTGGAGGGTGGAATTAAGGGTATAGGAAAGACT; TCTACAAATTCTTCAAAAAATCTCTCTGAATCACCAGTAATAACGAAAGCAAAAGAAGGG; TGTCAGAGTGACTCGATGAGACAAGAACAGAGCCCAAATGCAAATAATGATCAACCTGAG; GACTTGATTCAGGGATGTTCAGAAAGTGATTCCTCAGTTCTTAGAATGAGTGATCCTAGT; CATACCACAAACAAACTTTATCCAAAAGATCGAGTGTTAGATGATGTCTCCATTCGGAGC; CCAGAAACAAAATGTCCGAAACAAAATTCCATTGAAAATGACATAGAAGAAAAAGTCTCT; GACCTTGCCAGTAGAGGCCAGGAACCCAGTAAGAGTAAAACAAAAGGAAATGATTTTTTC; ATCGATGACTCTAAACTAGCCAGTGCAGATGATATTGGTACTTTGATCTGTAAGAACAAA; AAACCGCTCATACAGGAGGAAAGTGACACCATTGTTTCTTCTTCCAAGAGTGCTTTACAT; TCATCAGTGCCTAAAAGTACCAATGACAGAGATGCCACACCTCTGTCAAGAGCAATGGAC; TTTGAAGGAAAACTGGGATGTGACTCTGAATCTAATAGCACTTTGGAAAATAGTTCTGAT; ACCGTGTCTATTCAGGATAGCAGTGAAGAAGATATGATTGTTCAGAATAGCAATGAAAGC; ATTTCTGAACAGTTCAGAACTCGAGAACAAGATGTTGAAGTCTTGGAGCCGTTAAAGTGT; GAGTTGGTTTCTGGTGAGTCCACTGGAAACTGTGAGGACAGGCTGCCGGTCAAGGGGACT; GAAGCAAATGGTAAAAAACCAAGTCAGCAGAAGAAATTAGAGGAGAGACCAGTTAATAAA; TGTAGTGATCAAATAAAGCTAAAAAATACCACTGACAAAAAGAATAATGAAAATCGAGAG; TCTGAAAAGAAAGGACAGAGAACAAGTACATTTCAAATAAATGGAAAAGATAATAAACCC; AAAATATATTTGAAAGGTGAATGCTTGAAAGAAATTTCTGAGAGTAGAGTAGTAAGTGGT; AATGTTGAACCAAAGGTTAATAATATAAATAAAATAATCCCTGAGAATGATATTAAATCA; TTGACTGTTAAAGAATCTGCTATAAGGCCATTCATTAATGGTGATGTCATCATGGAAGAT; TTTAATGAAAGAAACAGCTCCGAAACAAAATCGCATTTGCTGAGTTCTTCAGATGCTGAA; GGTAACTACCGAGATAGCCTTGAGACCCTGCCATCAACCAAAGAGTCTGACAGTACACAG; ACGACCACACCCTCAGCATCTTGTCCAGAAAGCAATTCAGTTAATCAGGTAGAAGATATG; GAAATAGAAACCTCAGAAGTTAAGAAAGTTACTTCATCACCTATTACTTCTGAAGAGGAA; TCTAATCTCAGTAATGACTTTATTGATGAAAATGGTCTGCCCATCAACAAAAATGAAAAT; GTCAATGGAGAATCTAAAAGAAAAACCGTCATCACAGAAGTCACCACGATGACCTCCACA; GTGGCCACAGAATCAAAAACTGTGATCAAGGTAGAAAAAGGCGATAAGCAAACTGTGGTT; TCTTCCACAGAAAATTGTGCAAAATCCACTGTCACAACCACCACTACAACAGTGACCAAG; CTTTCCACACCCTCCACAGGCGGCAGTGTGGACATCATCTCTGTAAAGGAGCAGAGCAAA; ACCGTGGTCACCACGACAGTGACAGACTCCCTGACCACCACGGGAGGCACACTGGTTACA; TCTATGACTGTGAGCAAAGAGTATTCCACACGAGACAAAGTGAAACTGATGAAATTTTCA; AGACCAAAGAAGACTCGTTCAGGTACAGCTCTGCCATCCTATAGAAAATTTGTTACCAAG; AGCAGCAAGAAGAGCATTTTTGTTTTGCCTAATGATGACTTAAAAAAGTTGGCCCGAAAA; GGAGGAATCCGAGAGGTCCCTTATTTTAATTACAATGCAAAACCTGCTTTGGATATATGG; CCATATCCTTCTCCTAGACCGACCTTTGGCATCACTTGGAGGTATAGACTTCAGACAGTA; AAGTCCTTAGCTGGAGTGAGCCTGATGTTACGGTTACTGTGGGCAAGTTTGAGATGGGAT; GATATGGCGGCCAAGGCTCCTCCAGGAGGAGGGACTACACGGACAGAAACATCCGAAACT; GAAATCACAACAACAGAAATAATTAAGAGGAGAGATGTTGGTCCTTATGGCATTCGATCT; GAATATTGTATCAGGAAAATCATTTGTCCCATTGGAGTTCCAGAAACACCAAAAGAAACG; CCTACACCTCAGAGGAAAGGCCTTCGATCAAGTGCACTGCGGCCAAAGAGACCAGAAACG; CCCAAGCAAACTGGCCCTGTTATTATTGAAACCTGGGTAGCAGAAGAAGAACTGGAATTG; TGGGAGATCAGGGCATTTGCTGAGAGAGTGGAGAAAGAAAAGGCACAAGCAGTTGAGCAA; CAGGCTAAGAAACGACTGGAGCAGCAGAAGCCGACAGTGATTGCAACTTCCACTACTTCC; CCAACAAGCAGTACAACCAGCACCATCTCTCCAGCACAGAAGGTTATGGTGGCCCCCATA; AGTGGCTCAGTTACAACTGGAACCAAAATGGTACTAACTACTAAAGTTGGATCTCCAGCT; ACAGTAACATTCCAACAAAACAAGAACTTTCATCAAACCTTTGCTACATGGGTTAAGCAA; GGCCAGTCAAATTCAGGCGTTGTTCAAGTACAGCAGAAAGTCCTGGGTATCATTCCATCA; AGTACAGGTACCAGTCAGCAAACCTTTACTTCATTCCAGCCCAGGACAGCAACAGTCACA; ATTAGGCCCAATACCTCAGGCTCTGGAGGAACCACAAGCAATTCACAAGTAATCACAGGG; CCTCAGATTCGCCCTGGTATGACCGTGATTAGAACACCACTCCAACAGTCAACACTAGGA; AAGGCAATTATTCGAACACCTGTGATGGTACAGCCAGGTGCTCCTCAGCAAGTGATGACT; CAAATCATCAGGGGGCAGCCTGTCTCCACTGCAGTCTCCGCCCCTAACACGGTTTCCTCA; ACACCTGGGCAGAAAAGCTTAACTTCAGCAACGTCCACTTCAAATATACAGTCTTCAGCC; TCACAACCCCCTCGCCCCCAACAAGGACAAGTGAAGCTCACCATGGCTCAACTTACTCAG; TTAACACAGGGCCACGGTGGCAATCAAGGTTTGACAGTAGTAATTCAAGGACAAGGTCAA; ACTACTGGACAGTTGCAGTTGATACCTCAAGGGGTGACTGTACTCCCAGGCCCAGGCCAG; CAGCTAATGCAAGCTGCAATGCCAAATGGTACTGTTCAGCGATTCCTCTTTACCCCATTG; GCAACAACAGCCACCACAGCCAGCACCACCACCACCACTGTTTCCACGACAGCAGCAGGT; ACAGGTGAACAAAGGCAGAGTAAACTGTCACCCCAGATGCAGGTACATCAAGACAAAACC; CTGCCACCAGCTCAGTCATCAAGTGTGGGTCCAGCAGAAGCCCAGCCACAGACTGCTCAG; CCTTCAGCTCAGCCCCAGCCCCAAACCCAGCCCCAGTCCCCAGCTCAGCCTGAAGTTCAG; ACTCAGCCTGAAGTTCAGACCCAAACAACTGTTTCATCCCATGTCCCTTCTGAAGCACAA; CCCACCCACGCACAGTCATCCAAGCCCCAAGTTGCAGCACAGTCTCAGCCTCAAAGTAAT; GTCCAAGGACAGTCTCCTGTTCGTGTCCAAAGTCCATCACAGACTCGAATACGTCCATCA; ACTCCATCCCAACTGTCTCCTGGACAACAATCCCAGGTTCAGACTACAACCTCACAACCG; ATTCCAATTCAACCACATACATCTCTTCAGATACCTTCCCAAGGCCAGCCACAGTCACAA; CCCCAGGTGGTGATGAAGCATAATGCTGTAATAGAACATTTAAAACAGAAAAAGAGCATG; ACTCCAGCTGAAAGAGAAGAGAATCAAAGAATGATTGTCTGTAACCAGGTGATGAAGTAT; ATTTTGGATAAGATAGATAAAGAAGAAAAACAGGCAGCAAAAAAACGGAAGCGTGAAGAG; AGTGTGGAGCAGAAACGTAGCAAGCAGAATGCCACTAAGCTGTCAGCTCTGCTCTTCAAG; CACAAAGAGCAGCTCAGAGCCGAGATCCTGAAGAAGAGAGCACTCCTGGACAAGGATCTG; CAAATTGAAGTGCAGGAAGAGCTGAAGAGAGACCTGAAAATTAAGAAAGAAAAAGACCTG; ATGCAGTTGGCTCAGGCCACAGCAGTAGCTGCACCCTGCCCCCCAGTGACACCAGCTCCT; CCAGCCCCTCCAGCCCCTCCACCTTCACCTCCCCCTCCACCTGCTGTGCAACACACAGGC; CTTCTGTCCACGCCCACCTTACCTGCTGCTTCCCAGAAGAGGAAGCGGGAAGAGGAAAAA; GACTCCAGCTCAAAGTCCAAGAAAAAGAAAATGATCTCTACTACCTCAAAGGAAACTAAG; AAGGACACAAAGCTTTACTGTATCTGTAAAACGCCTTATGATGAATCTAAATTTTATATT; GGCTGTGATCGGTGTCAGAATTGGTACCATGGGCGCTGCGTTGGCATCTTGCAAAGTGAG; GCAGAGCTCATTGATGAGTATGTCTGTCCACAGTGCCAGTCAACAGAGGATGCCATGACA; GTGCTCACGCCACTAACAGAGAAGGATTATGAGGGGTTGAAGAGGGTGCTCCGTTCCTTA; CAGGCCCATAAGATGGCCTGGCCTTTCCTTGAACCAGTAGACCCTAATGATGCACCAGAT; TATTATGGTGTTATTAAGGAACCTATGGACCTTGCCACCATGGAAGAAAGAGTACAAAGA; CGATATTATGAAAAGCTGACGGAATTTGTGGCAGATATGACCAAAATTTTTGATAACTGT; CGTTACTACAATCCAAGTGACTCCCCATTTTACCAGTGTGCAGAAGTTCTCGAATCATTC; TTTGTACAGAAATTGAAAGGCTTCAAAGCTAGCAGGTCTCATAACAACAAACTGCAGTCT; ACAGCTTCTTAA;
• Restriction Sites: Please inquire
• ACCN: NM_004459
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.; ; The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_004459.5, NP_004450.3
• RefSeq Size: 10884 bp
• RefSeq ORF: 10863 bp
• Locus ID: 2186
• UniProt ID: Q12830
• Cytogenetics: 17q24.2
• Domains: DDT, PHD
• Protein Families: Druggable Genome
• Summary: This gene was identified by the reactivity of its encoded protein to a monoclonal antibody prepared against brain homogenates from patients with Alzheimer's disease. Analysis of the original protein (fetal Alz-50 reactive clone 1, or FAC1), identified as an 810 aa protein containing a DNA-binding domain and a zinc finger motif, suggested it might play a role in the regulation of transcription. High levels of FAC1 were detected in fetal brain and in patients with neurodegenerative diseases. The protein encoded by this gene is actually much larger than originally thought, and it also contains a C-terminal bromodomain characteristic of proteins that regulate transcription during proliferation. The encoded protein is highly similar to the largest subunit of the Drosophila NURF (nucleosome remodeling factor) complex. In Drosophila, the NURF complex, which catalyzes nucleosome sliding on DNA and interacts with sequence-specific transcription factors, is necessary for the chromatin remodeling required for transcription. Two alternative transcripts encoding different isoforms have been described completely. [provided by RefSeq, Jul 2008]; Transcript Variant: This variant (2) contains two additional in-frame exons in the 5' coding region and uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. The encoded isoform (2) is shorter than isoform 1.