Synaptotagmin VII (SYT7) (NM_004200) Human Untagged Clone

SKU SC309400

SYT7 (untagged)-Human synaptotagmin VII (SYT7)

Product Data

• Type: Human Untagged Clone
• Target Symbol: Synaptotagmin VII
• Synonyms: IPCA-7; IPCA7; PCANAP7; SYT-VII; SYTVII
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_004200 edited; CTCTGGGAGCCCGGAGGGAACCGGCGACGAGGGGACCATGTACCGGGACCCGGAGGCGGC; CAGCCCAGGGGCGCCCTCGCGCGACGTCCTGCTGGTCTCTGCCATCATCACCGTCAGCCT; TAGCGTCACTGTCGTCCTCTGCGGCCTCTGCCACTGGTGTCAGCGCAAACTGGGCAAACG; CTACAAGAATTCCTTGGAGACGGTGGGCACGCCAGACTCAGGGCGTGGGCGCAGTGAGAA; GAAGGCTATCAAGTTGCCTGCAGGAGGGAAGGCGGTGAACACAGCCCCCGTGCCAGGCCA; GACACCCCACGATGAGTCCGACCGCCGGACCGAGCCACGTTCCTCCGTCTCAGACCTCGT; CAACTCCCTCACCAGCGAGATGCTCATGCTCTCCCCAGGCTCCGAGGAGGATGAGGCCCA; CGAGGGTTGCAGCCGAGAGAACCTGGGCCGGATCCAGTTCAGTGTCGGCTACAACTTCCA; GGAGTCCACGCTCACCGTGAAGATCATGAAGGCCCAGGAGCTGCCGGCCAAGGACTTCAG; CGGCACCAGCGACCCCTTCGTCAAGATCTACCTGCTGCCCGACAAGAAGCACAAGCTGGA; GACCAAGGTGAAGCGGAAGAACCTGAACCCCCACTGGAACGAGACCTTCCTCTTTGAAGG; TTTTCCCTATGAGAAGGTGGTGCAGAGGATCCTCTACCTCCAAGTCCTGGACTATGACCG; CTTCAGCCGCAACGACCCCATTGGGGAGGTGTCCATCCCCCTTAACAAGGTGGACCTGAC; CCAGATGCAGACCTTCTGGAAGGATCTGAAGCCATGCAGCGATGGGAGTGGGAGCCGAGG; GGAGCTGCTCTTGTCTCTCTGCTACAACCCCTCTGCCAACTCCATCATCGTGAACATCAT; CAAAGCCCGGAACCTCAAAGCCATGGACATCGGGGGCACATCAGACCCCTACGTGAAGGT; ATGGCTGATGTACAAGGACAAGCGGGTGGAGAAGAAGAAGACGGTGACGATGAAGAGGAA; CCTGAACCCCATCTTCAATGAGTCCTTCGCCTTCGATATCCCCACGGAGAAGCTGAGGGA; GACGACCATCATCATCACTGTCATGGACAAGGACAAGCTCAGCCGCAATGACGTCATCGG; CAAGATCTACCTGTCCTGGAAGAGCGGGCCAGGGGAGGTGAAGCACTGGAAGGACATGAT; TGCCCGTCCCCGGCAGCCCGTGGCCCAGTGGCACCAGCTGAAGGCCTGAGTGGGGCCAAG; GGAGGCCCAGGGGGCCGAGGGCCCAGGTCCCCATCATGCCCTCACCACTTTATGCACAAC; GCCCGGCCTGAGCCCCCTGCCATAGGGAGGGGAGGACCCTGAGGGCTCAGCCAGGGAGGG; GTCCCAGGACTCAACTGGGCCCCGTTTCTAGGAAGTACCAGCCCCATCCCCCACAGTCCA; GCTCCGGGGAAGGGGCTCTGGGAGGCATTTTCCTGCTTTGCCCCTTTTCTTCCTGACTTA; CTAATACTAAAGAAGTTGGGGGAGCTCGAGAGCCAGACGGCCAGACAGGCAGACCCCTCC; AGAGGCCCGCCAGGTGGGCATGGTCCCCCATTTTCTTTAAGGCAGCACCTGGAGTGGAGA; GAGGCCACTCCCTCTCCAGCCCCCGATGTGGACCCGGGGAGGGGAGGCTGAGGCGTTTGG; CCCCGGCCTGGCCAGGAGAGGCCCATCCCCAGGGCAGTTTCAGGTGCCGGCTGGGCCCTG; AATGTCGAGGATAGTATATAGCCCGCTCCTGGGTCCTGGAGCTGTGGCCCTTTGTACTCG; TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA; AAAAAAAAAAAA;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004200 unedited; CCACAAGACCCCCNNCCCCCCTTCCCCCCCCCGGTTCAAATTTGTATACAACTCATATAG; GCGGCCGCGNATATCGCACCAGCTCTGNAGGAGCCCGGNAGGNAACCGGCGACGAGGGGA; CCATGTACCGGGACCCGGNAGCGGCCAGCCCAGGGCGCCCTCGCGCGACGTCCTGCTGGT; CTCTGCCATCATCACCGTCAGCCTTAGCGTCACTGTCGTCCTCTGCGGCCTCTGCCACTG; GTGTCAGCGCAAACTGGGCAAACGCTACAAGAATTCCTTGGAGACGGTGGGCACGCCAGA; CTCAGGGCGTGGGCGCAGTGAGAAGAAGGCTATCAAGTTGCCTGCAGGAGGGAAGGCGGT; GAACACAGCCCCCGTGCCAGGCCAGACACCCCACGATGAGTCCGACCGCCGGACCGAGCC; ACGTTCCTCCGTCTCAGACCTCGTCAACTCCCTCACCAGCGAGATGCTCATGCTCTCCCC; AGGCTCCGAGGAGGATGAGGCCCACGAGGGTTGCAGCCGAGAGAACCTGGGCCGGATCCA; GTTCAGTGTCGGCTACAACTTCCAGGAGTCCACGCTCACCGTGAAGATCATGAAGGCCCA; GGAGCTGCCGGCCAAGGACTTCAGCGGCACCAGCGACCCCTTCGTCAAGATCTACCTGCT; GCCCGACAAGAGCACAAGCTGGAGACCAAGGTGAAGCGGAAGAACCTGAACCCCCACTGG; AACGAGACCTTCCTCTNTGAAGGGTNTCCCTATGAAAGGGTGGTGCANGAGATCCTCTAC; CTNCAAGTCCTGGACTATGACCGCTTCAGCCGCAACGACCCCATTGGGGAGGTGTCCATC; CCCCCTTACCAAGGTGGACCTGACCCAGAGCAGACCTTCTGGAAGGATCTGAACCATGCA; CCGATGGGAGTGGGAGCCCGAGGGGAGCCGTCTGGNNTCTCTGCTAACACCCCTTTGCCA; CTCCTCATNGG;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004200 unedited; GGACTAACTATGGACCGTAGCCCCCCATCTANGATCGAGTTTAAAACCCTTTTTTTTTTT; TTTTTTTTTTTTTTTTTTTTTTTTTTTTTNTTTTTTTTTTTTTTTTTTTTTTTTTAACAA; AAACAAAGGGCCACAGTTCCAGGACCCAAGAGCGGGCTTTTTTCTTTCCTCAAAATTTAG; GGCCCACCCGGCCCCTGAAAACTGCCCGGGGGATGGGCCTTTCCTGGCCAGGCCGGGGCC; AAAACCCTTAACCTCCCCTCCCCGGGTCCAAATTGGGGGCTGGAAAAGGAATGGCCCCTC; TCCACTCCAGGGGCTGCCTTAAAAAAAATGGGGGACCATGCCCCCCTGGGGGGCCTTTGG; AGGGGTTTGCCTGTTTGGCCCTTTGGCTTTTAAACCCCCCCAATTTTTTTATTTTTAATA; AGTCAGGAAAAAAAGGGGCAAAACAGGAAAATGCCTCCCAAAACCCCTTCCCCGGAACTG; GACTGGGGGGGATGGGGCTGGTACTTCCTAAAAACGGGGCCCATTTGAGTCCTGGGACCC; CTCCCTGGGTGAACCCTCAGGGGCCCCCCCTCCCTATGGGAGGGGGCTCAAGCCGGGCGT; TGTGCATAAAGTGGGGAGGGCATGATGGGGACCTGGGCCCTCGGCCCCCTGGGCCTCCCT; TGGCCCCATTTAAGCCTTTAATTGGGGCCACTGGGCCACGGGCTTCCGGGGACAGGCAAT; CATGTCCTTCCAAGGGTTAACCTCCCCTGGGCCGGTTTTTCAGGAAGGTAGATCTTGCCG; ATAAACTCATTGCGGCTGAACTTGGCCTTGTCCATGAAAAGGATGAATGATGGCCGCCTC; CCTCACCTTCTCCCGGGGGATATCCAAGGCGAAGGACTCTTGGAAAAGGC;
• Restriction Sites: NotI-NotI
• ACCN: NM_004200
• Insert Size: 1900 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_004200.2, NP_004191.2
• RefSeq Size: 4609 bp
• RefSeq ORF: 1212 bp
• Locus ID: 9066
• UniProt ID: O43581
• Cytogenetics: 11q12.2
• Protein Families: Secreted Protein, Transmembrane
• Summary: This gene is a member of the synaptotagmin gene family and encodes a protein similar to other family members that mediate calcium-dependent regulation of membrane trafficking in synaptic transmission. A similar protein in rodents mediates hormone secretion and lysosome exocytosis. In humans, expression of this gene has been associated with prostate cancer. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Oct 2011]; Transcript Variant: This variant (2) lacks an exon in the coding region but maintains the reading frame, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.