Nicotinic Acetylcholine Receptor beta 2 (CHRNB2) (NM_000748) Human Untagged Clone

SKU SC309051

CHRNB2 (untagged)-Human cholinergic receptor, nicotinic, beta 2 (neuronal) (CHRNB2)

Product Data

• Type: Human Untagged Clone
• Target Symbol: Nicotinic Acetylcholine Receptor beta 2
• Synonyms: EFNL3; nAChRB2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_000748 edited; CCCAGGAACCACCGCGGCGGCCGGCACCACCTGGACCCAGCTCCAGGCGGGCGCGGCTTC; AGCACCACGGACAGCGCCCCACCCGCGGCCCTCCCCCCGGCGGCGCGCTCCAGCCGGTGT; AGGCGAGGCAGCGAGCTATGCCCGCGGCATGGCCCGGCGCTGCGGCCCCGTGGCGCTGCT; CCTTGGCTTCGGCCTCCTCCGGCTGTGCTCAGGGGTGTGGGGTACGGATACAGAGGAGCG; GCTGGTGGAGCATCTCCTGGATCCTTCCCGCTACAACAAGCTTATCCGCCCAGCCACCAA; TGGCTCTGAGCTGGTGACAGTACAGCTTATGGTGTCACTGGCCCAGCTCATCAGTGTGCA; TGAGCGGGAGCAGATCATGACCACCAATGTCTGGCTGACCCAGGAGTGGGAAGATTATCG; CCTCACCTGGAAGCCTGAAGAGTTTGACAACATGAAGAAAGTTCGGCTCCCTTCCAAACA; CATCTGGCTCCCAGATGTGGTCCTGTACAACAATGCTGACGGCATGTACGAGGTGTCCTT; CTATTCCAATGCCGTGGTCTCCTATGATGGCAGCATCTTCTGGCTGCCGCCTGCCATCTA; CAAGAGCGCATGCAAGATTGAAGTAAAGCACTTCCCATTTGACCAGCAGAACTGCACCAT; GAAGTTCCGTTCGTGGACCTACGACCGCACAGAGATCGACTTGGTGCTGAAGAGTGAGGT; GGCCAGCCTGGACGACTTCACACCTAGTGGTGAGTGGGACATCGTGGCGCTGCCGGGCCG; GCGCAACGAGAACCCCGACGACTCTACGTACGTGGACATCACGTATGACTTCATCATTCG; CCGCAAGCCGCTCTTCTACACCATCAACCTCATCATCCCCTGTGTGCTCATCACCTCGCT; AGCCATCCTTGTCTTCTACCTGCCATCCGACTGTGGCGAGAAGATGACGTTGTGCATCTC; AGTGCTGCTGGCGCTCACGGTCTTCCTGCTGCTCATCTCCAAGATCGTGCCTCCCACCTC; CCTCGACGTGCCGCTCGTCGGCAAGTACCTCATGTTCACCATGGTGCTTGTCACCTTCTC; CATCGTCACCAGCGTGTGCGTGCTCAACGTGCACCACCGCTCGCCCACCACGCACACCAT; GGCGCCCTGGGTGAAGGTCGTCTTCCTGGAGAAGCTGCCCGCGCTGCTCTTCATGCAGCA; GCCACGCCATCATTGCGCCCGTCAGCGCCTGCGCCTGCGGCGACGCCAGCGTGAGCGCGA; GGGCGCTGGAGCCCTCTTCTTCCGCGAAGCCCCAGGGGCCGACTCCTGCACGTGCTTCGT; CAACCGCGCGTCGGTGCAGGGGTTGGCCGGGGCCTTCGGGGCTGAGCCTGCACCAGTGGC; GGGCCCCGGGCGCTCAGGGGAGCCGTGTGGCTGTGGCCTCCGGGAGGCGGTGGACGGCGT; GCGCTTCATCGCAGACCACATGCGGAGCGAGGACGATGACCAGAGCGTGAGTGAGGACTG; GAAGTACGTCGCCATGGTGATCGACCGCCTCTTCCTCTGGATCTTTGTCTTTGTCTGTGT; CTTTGGCACCATCGGCATGTTCCTGCAGCCTCTCTTCCAGAACTACACCACCACCACCTT; CCTCCACTCAGACCACTCAGCCCCCAGCTCCAAGTGAGGCCCTTCCTCATCTCCATGCTC; TTTCTCGAGCGGAAGGGCGAATTCAGATCTGGTACCGATATCAAGCTTGTCGACTCTAGA; TTGCGGCCGCGGTCATAGCTGTTTC;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000748 unedited; GGGGGTGTTCAGATATTTGTAAACGACTTACTATAGGGNNCGGCCGCGCAATTCCTCATA; CCAGGATAGGCAAGAAGCTGGTTTCTCCTCGCAGCCGGCTCCCTGAGGCCCAGGAACCAC; CGCGGCGGCCGGCACCACCTGGACCCAGCTCCAGGCGGGCGCGGCTTCAGCACCACGGAC; AGCGCCCCACCCGCGGCCCTCCCCCCGGCGGCGCGCTCCAGCCGGTGTAGGCGAGGCAGC; GAGCTATGCCCGCGGCATGGCCCGGCGCTGCGGCCCCGTGGCGCTGCTCCTTGGCTTCGG; CCTCCTCCGGCTGTGCTCAGGGGTGTGGGGTACGGATACAGAGGAGCGGCTGGTGGAGCA; TCTCCTGGATCCTTCCCGCTACAACAAGCTTATCCGCCCAGCCACCAATGGCTCTGAGCT; GGTGACAGTACAGCTTATGGTGTCACTGGCCCAGCTCATCAGTGTGCATGAGCGGGAGCA; GATCATGACCACCAATGTCTGGCTGACCCAGGAGTGGGAAGATTATCGCCTCACCTGGAA; GCCTGAAGAGTTTGACAACATGAAGAAAGTTCGGCTCCCTTCCAAACACATCTGGCTCCC; AGATGTGGTCCTGTACAACAATGCTGACGGCATGTACGAGGTGTCCTTCTATTCCAATGC; CGTGGTCTCCTATGATGGCAGCATCTTCTGGCTGCCGCCTGCCATCTACAAGAGCGCATG; CAAGATTGAAGTAAAGCACTTCCCATTTGACCAGCAGAACTGCACCATGAAGTTCCGTTC; GTGGACCTACGACCGCACAGAGATCGACTTGGTGCTGAAGAGTGAGGTGGCCAGCCTGGA; CGACTTCACACCTAGTGGTGAGTGGGACATCGTGGCGCTGCCGGGCCGGCGCAACGAGAA; CCCCGACGACTCTACG;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000748 unedited; CTGGGAGGCACTTCAGGCCAGAGAGATGGGAGGGTCACAGGGTGCCCCCGGGATCGTTCA; GAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGACAAGCTTGATATCGGTACCAGATC; TGAATTCGCCCTTCCGCTCGAGAAAGAGCATGGAGATGAGGAAGGGCCTCACTTGGAGCT; GGGGGCTGAGTGGTCTGAGTGGAGGAAGGTGGTGGTGGTGTAGTTCTGGAAGAGAGGCTG; CAGGAACATGCCGATGGTGCCAAAGACACAGACAAAGACAAAGATCCAGAGGAAGAGGCG; GTCGATCACCATGGCGACGTACTTCCAGTCCTCACTCACGCTCTGGTCATCGTCCTCGCT; CCGCATGTGGTCTGCGATGAAGCGCACGCCGTCCACCGCCTCCCGGAGGCCACAGCCACA; CGGCTCCCCTGAGCGCCCGGGGCCCGCCACTGGTGCAGGCTCAGCCCCGAAGGCCCCGGC; CAACCCCTGCACCGACGCGCGGTTGACGAAGCACGTGCAGGAGTCAGCCCCTGGGGCTTC; GCGGAAGAAGAGGGCTCCAGCGCCCTCGCGCTCACGCTGGCGTCGCCGCAGGCGCAAGCG; CTGACGGGCGCAATGATGGCGTGGCTGCTGCATGAAGAGCAGCGCGGGCAGCTTCTCCAG; GAAGACGACCTTCACCCATGGCGCCATGGTGTGGCTTGGTGGCCGACCGGTGGTGCACGT; TTAAGCCGCCCCACGCTGGTGACCAATGGAGAAGGTGACCAAGCCCCATGGTGGACATTG; AGGTAACTTGCCGCCCAAGCGGCACGTCCAG;
• Restriction Sites: Please inquire
• ACCN: NM_000748
• Insert Size: 1800 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_000748.1, NP_000739.1
• RefSeq Size: 2448 bp
• RefSeq ORF: 1509 bp
• Locus ID: 1141
• UniProt ID: P17787
• Cytogenetics: 1q21.3
• Domains: Neur_chan_LBD, Neur_chan_memb
• Protein Families: Druggable Genome, Ion Channels: Cys-loop Receptors, Transmembrane
• Summary: Neuronal acetylcholine receptors are homo- or heteropentameric complexes composed of homologous alpha and beta subunits. They belong to a superfamily of ligand-gated ion channels which allow the flow of sodium and potassium across the plasma membrane in response to ligands such as acetylcholine and nicotine. This gene encodes one of several beta subunits. Mutations in this gene are associated with autosomal dominant nocturnal frontal lobe epilepsy. [provided by RefSeq, Jul 2008]