Cadherin 7 (CDH7) (NM_004361) Human Untagged Clone

SKU SC308991

CDH7 (untagged)-Human cadherin 7, type 2 (CDH7), transcript variant b

Product Data

• Type: Human Untagged Clone
• Target Symbol: Cadherin 7
• Synonyms: CDH7L1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_004361 edited; ATGAAGTTGGGCAAAGTGGAGTTCTGCCATTTTCTGCAGCTAATAGCTCTTTTCCTGTGT; TTTTCTGGGATGAGTCAAGCAGAACTCTCAAGGTCCAGATCAAAGCCCTATTTCCAATCA; GGGAGGTCCCGGACCAAGCGCAGCTGGGTGTGGAATCAGTTCTTTGTGCTGGAGGAATAC; ATGGGTTCAGACCCCCTCTATGTAGGAAAGCTTCACTCTGATGTTGATAAAGGAGATGGT; TCCATCAAATACATCTTGTCAGGCGAAGGGGCAAGTTCCATTTTCATTATTGATGAGAAC; ACTGGGGATATTCATGCCACCAAGAGACTGGATCGTGAGGAGCAGGCCTACTACACGCTC; CGAGCTCAAGCGCTGGATAGGCTCACCAACAAACCCGTGGAGCCCGAGTCGGAGTTTGTC; ATCAAAATTCAGGATATCAACGACAATGAACCCAAATTTTTGGATGGCCCATACACGGCA; GGAGTTCCCGAAATGTCTCCCGTGGGGACCTCAGTGGTACAAGTGACAGCGACGGATGCT; GATGATCCTACATATGGCAACAGTGCCAGAGTGGTCTACAGTATTCTGCAAGGACAGCCG; TACTTCTCAGTGGAGCCAAAGACAGGAGTCATCAAGACTGCCCTTCCAAACATGGATAGA; GAGGCTAAAGACCAGTATTTGCTTGTTATTCAGGCAAAGGATATGGTTGGTCAAAATGGA; GGACTGTCAGGAACTACATCAGTCACTGTGACCCTAACTGATGTCAACGATAATCCACCT; CGCTTTCCTCGAAGGTCTTATCAATATAACGTCCCAGAGTCATTACCTGTAGCCTCAGTT; GTGGCCAGAATTAAAGCTGCTGATGCAGATATTGGAGCTAATGCTGAAATGGAGTACAAG; ATTGTGGATGGTGATGGTTTGGGCATTTTTAAGATTTCTGTTGACAAAGAAACCCAGGAA; GGAATCATTACTATACAGAAGGAGCTGGATTTTGAAGCCAAAACAAGTTACACGCTACGG; ATAGAAGCTGCAAATAAAGATGCCGACCCTCGCTTTCTGAGCTTGGGTCCGTTCAGTGAC; ACGACAACTGTGAAGATAATTGTGGAAGATGTAGATGAGCCCCCTGTGTTCTCTTCACCC; TTGTACCCTATGGAGGTGTCGGAAGCTACCCAGGTTGGGAATATCATTGGCACTGTAGCA; GCTCATGACCCAGATTCTTCCAATAGCCCTGTGAGGTACTCAATTGACAGAAACACAGAC; TTGGAGAGATACTTCAATATTGATGCCAACAGTGGGGTCATCACAACTGCCAAGTCTTTG; GATCGAGAGACAAATGCTATTCACAATATCACAGTCCTTGCAATGGAGAGCCAGAATCCA; TCTCAAGTAGGAAGAGGCTATGTGGCCATCACTATACTTGACATCAATGATAACGCCCCT; GAATTTGCCATGGACTATGAGACCACCGTCTGTGAAAATGCCCAGCCGGGGCAGGTTATC; CAGAAAATCAGTGCTGTGGATAAAGATGAGCCATCCAATGGACACCAGTTTTACTTCAGC; TTAACAACGGATGCAACAAATAACCACAACTTTTCATTGAAAGATAACAAAGACAACACA; GCCTCAATACTGACCAGGAGAAACGGCTTCCGGAGACAGGAACAATCAGTTTACTATCTG; CCAATTTTCATTGTGGACAGTGGATCTCCCTCACTTAGCAGCACCAGCACCCTCACCATC; CGCGTGTGTGACTGTGATGCTGACGGCGTAGCCCAGACCTGCAATGCAGAGGCCTATGTC; CTACCTGCTGGCCTCAGTACAGGAGCCCTGATAGCCATACTCGCCTGTGTCTTGACATTA; TTGGTGTTGATCCTCCTTATCGTCACTATGAGAAGACGGAAAAAAGAGCCCCTTATTTTT; GACGAAGAAAGAGACATCAGAGAAAATATTGTGAGATATGATGACGAGGGCGGGGGAGAG; GAGGACACGGAAGCGTTTGACATGGCTGCACTGAGAAACCTCAACGTCATCCGAGACACC; AAGACCCGGAGGGATGTGACTCCAGAAATTCAATTCCTGAGTCGACCAGCTTTTAAAAGC; ATCCCAGATAATGTCATCTTTAGGGAATTTATTTGGGAAAGATTAAAAGAAGCCGATGTT; GATCCTGGTGCTCCTCCTTATGACTCCCTGCAGACATATGCTTTTGAAGGAAATGGCTCA; GTTGCTGAATCACTCAGCTCTTTAGATTCCATCAGCTCAAACTCTGATCAGAACTATGAC; TACCTAAGTGACTGGGGACCTCGCTTTAAACGACTCGCGGACATGTATGGGACTGGCCAA; GAGAGTTTGTACTCATAG;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004361 unedited; NGCGTTCAGATATTTGTAATACGACTTACTATAGGCGGCCGCGCAATTCGCACGAGGCGG; AGCTGCGCGCACTGGGTCCCCAAAAGCCCGCGGGCGTCCGGCAGCCGAGCGCACGTTCTT; TCGGATGCACACGCCCGGGTCCCTGGCGTCTGACGCCGTGGGGAGGGCAGCGAGGCCCCA; GGTACTTACTACACCATTCTTTGGCGAAGGCTATTCAGCAGTGGTGACCTCTTCCAATCC; AACACTCTACAGATTATTATCTCTGGACTCCCAGCTGACACCCTGCCGGAGGCAAGAGCT; ACTAAGCCAACTGGAACTGTGCCTTTTCTCTTGTCAAGGTTTTTTTCTTACACAGGAAAA; AGAAAGAAAAAAAAAAGATGAAGTTGGGCAAAGTGGAGTTCTGCCATTTTCTGCAGCTAA; TAGCTCTTTTCCTGTGTTTTTCTGGGATGAGTCAAGCAGAACTCTCAAGGTCCAGATCAA; AGCCCTATTTCCAATCAGGGAGGTCCCGGACCAAGCGCAGCTGGGTGTGGAATCAGTTCT; TTGTGCTGGAGGAATACATGGGTTCAGACCCCCTCTATGTAGGAAAGCTTCACTCTGATG; TTGATAAAGGAGATGGTTCCATCAAATACATCTTGTCAGGCGAAGGGGCAAGTTCCATTT; TCATTATTGATGAGAACACTGGGGATATTCATGCCACCAAGAGACTGGATCGTGAGGAGC; AGGCCTACTACACGCTNCGAGCTCAAGCGCTGGATAGGCTCACCAACAACCCGTGGAGCC; CGAGTTCGAGTTTGTCATCAAAAATCAGGATATCAAACGACATGAACCCAAATTTTTGGA; TGGCCATACACGGCAAGAGTTCCCGAAATGTCTCCCGTGGGGACCTCAGTGGTACAAGTG; ACAGCGACGGATGCTG;
  3' Read Nucleotide Sequence

  >Forward primer walk for NM_004361 unedited; GTTTTTGCATTGCANCTTGACAACGGAAGNCAACAAATAACCACAACTTTNTCATTGAAG; AAAACAAAGACAACACAGCCTCATACTGACCAGAGAAACGGCTTCCGGAGACAGGAACAA; TCAGTTTACTATCTGCCAATTTTCATTGTGGACAGTGGATCTCCCTCACTTAGCAGCACC; AGCACCCTCACCATCCGCGTGTGTGACTGTGATGCTGACGGCGTAGCCCAGACCTGCAAT; GCAGAGGCCTATGTCCTACCTGCTGGCCTCAGTACAGGAGCCCTGATAGCCATACTCGCC; TGTGTCTTGACATTATTGGTGTTGATCCTCCTTATCGTCACTATGAGAAGACGGAAAAAA; GAGCCCCTTATTTTTGACGAAGAAAGAGACATCAGAGAAAATATTGTGAGATATGATGAC; GAGGGCGGGGGAGAGGAGGACACGGAAGCGTTTGACATGGCTGCACTGAGAAACCTCAAC; GTCATCCGAGACACCAAGACCCGGAGGGATGTGACTCCAGAAATTCAATTCCTGAGTCGA; CCAGCTTTTAAAAGCATCCCAGATAATGTCATCTTTAGGGAATTTATTTGGGAAAGATTA; AAAGAAGCCGATGTTGATCCTGGTGCTCCTCCTTATGACTCCCTGCAGACATATGCTTTT; GAAGGAAATGGCTCAGTTGCTGAATCACTCAGCTCTTTAGATTCCATCAGCTCANACTCT; GATCAGAACTATGACTACCTAAGTGACTGGGGACCTCGCTTTAAACGACTCGCGGACATG; TATGGGACTGGCCAAGAGAGTTTGTACTCATAGCCTTGGAAACCTTAATTCGAAATGTAC; TGNNAGAAAAGTAACCGCNAAAAATAAATAAAATGAAATAAAAATATTAACCACTACCTA; CAGGAAACAAGAACTCCCCTTGCTC;
• Restriction Sites: NotI-NotI
• ACCN: NM_004361
• Insert Size: 3000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: There is 1 nucleotide difference between the OriGene clone and the NCBI reference ORF. These result in the substitution of 1 aa.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_004361.2, NP_004352.2
• RefSeq Size: 2828 bp
• RefSeq ORF: 2358 bp
• Locus ID: 1005
• UniProt ID: Q9ULB5
• Cytogenetics: 18q22.1
• Protein Families: Transmembrane
• Summary: This gene encodes a type II classical cadherin of the cadherin superfamily. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature glycoprotein. This calcium dependent cell-cell adhesion molecule is comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Type II (atypical) cadherins are defined based on their lack of a histidine-alanine-valine (HAV) cell adhesion recognition sequence specific to type I cadherins. Cadherins mediate cell-cell binding in a homophilic manner, contributing to the sorting of heterogeneous cell types. Mutations in this gene may be associated with bipolar disease in human patients. This gene is present in a gene cluster on chromosome 18. [provided by RefSeq, May 2016]; Transcript Variant: This variant (b) represents the longest transcript and encodes the longer isoform (1). Variants a, b and d encode the same isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.