CrkRS (CDK12) (NM_016507) Human Untagged Clone

SKU SC308894

CDK12 (untagged)-Human cyclin-dependent kinase 12 (CDK12), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: CrkRS
• Synonyms: CRK7; CRKR; CRKRS
• Vector: pCMV6 series
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_016507, the custom clone sequence may differ by one or more nucleotides; ATGCCCAATTCAGAGAGACATGGGGGCAAGAAGGACGGGAGTGGAGGAGCTTCTGGAACT; TTGCAGCCGTCATCGGGAGGCGGCAGCTCTAACAGCAGAGAGCGTCACCGCTTGGTATCG; AAGCACAAGCGGCATAAGTCCAAACACTCCAAAGACATGGGGTTGGTGACCCCCGAAGCA; GCATCCCTGGGCACAGTTATCAAACCTTTGGTGGAGTATGATGATATCAGCTCTGATTCC; GACACCTTCTCCGATGACATGGCCTTCAAACTAGACCGAAGGGAGAACGACGAACGTCGT; GGATCAGATCGGAGCGACCGCCTGCACAAACATCGTCACCACCAGCACAGGCGTTCCCGG; GACTTACTAAAAGCTAAACAGACCGAAAAAGAAAAAAGCCAAGAAGTCTCCAGCAAGTCG; GGATCGATGAAGGACCGGATATCGGGAAGTTCAAAGCGTTCGAATGAGGAGACTGATGAC; TATGGGAAGGCGCAGGTAGCCAAAAGCAGCAGCAAGGAATCCAGGTCATCCAAGCTCCAC; AAGGAGAAGACCAGGAAAGAACGGGAGCTGAAGTCTGGGCACAAAGACCGGAGTAAAAGT; CATCGAAAAAGGGAAACACCCAAAAGTTACAAAACAGTGGACAGCCCAAAACGGAGATCC; AGGAGCCCCCACAGGAAGTGGTCTGACAGCTCCAAACAAGATGATAGCCCCTCGGGAGCT; TCTTATGGCCAAGATTATGACCTTAGTCCCTCACGATCTCATACCTCGAGCAATTATGAC; TCCTACAAGAAAAGTCCTGGAAGTACCTCGAGAAGGCAGTCGGTCAGTCCCCCTTACAAG; GAGCCTTCGGCCTACCAGTCCAGCACCCGGTCACCGAGCCCCTACAGTAGGCGACAGAGA; TCTGTCAGTCCCTATAGCAGGAGACGGTCGTCCAGCTACGAAAGAAGTGGCTCTTACAGC; GGGCGATCGCCCAGTCCCTATGGTCGAAGGCGGTCCAGCAGCCCTTTCCTGAGCAAGCGG; TCTCTGAGTCGGAGTCCACTCCCCAGTAGGAAATCCATGAAGTCCAGAAGTAGAAGTCCT; GCATATTCAAGACATTCATCTTCTCATAGTAAAAAGAAGAGATCCAGTTCACGCAGTCGT; CATTCCAGTATCTCACCTGTCAGGCTTCCACTTAATTCCAGTCTGGGAGCTGAACTCAGT; AGGAAAAAGAAGGAAAGAGCAGCTGCTGCTGCTGCAGCAAAGATGGATGGAAAGGAGTCC; AAGGGTTCACCTGTATTTTTGCCTAGAAAAGAGAACAGTTCAGTAGAGGCTAAGGATTCA; GGTTTGGAGTCTAAAAAGTTACCCAGAAGTGTAAAATTGGAAAAATCTGCCCCAGATACT; GAACTGGTGAATGTAACACATCTAAACACAGAGGTAAAAAATTCTTCAGATACAGGGAAA; GTAAAGTTGGATGAGAACTCCGAGAAGCATCTTGTTAAAGATTTGAAAGCACAGGGAACA; AGAGACTCTAAACCCATAGCACTGAAAGAGGAGATTGTTACTCCAAAGGAGACAGAAACA; TCAGAAAAGGAGACCCCTCCACCTCTTCCCACAATTGCTTCTCCCCCACCCCCTCTACCA; ACTACTACCCCTCCACCTCAGACACCCCCTTTGCCACCTTTGCCTCCAATACCAGCTCTT; CCACAGCAACCACCTCTGCCTCCTTCTCAGCCAGCATTTAGTCAGGTTCCTGCTTCCAGT; ACTTCAACTTTGCCCCCTTCTACTCACTCAAAGACATCTGCTGTGTCCTCTCAGGCAAAT; TCTCAGCCCCCTGTACAGGTTTCTGTGAAGACTCAAGTATCTGTAACAGCTGCTATTCCA; CACCTGAAAACTTCAACGTTGCCTCCTTTGCCCCTCCCACCCTTATTACCTGGAGATGAT; GACATGGATAGTCCAAAAGAAACTCTTCCTTCAAAACCTGTGAAGAAAGAGAAGGAACAG; AGGACACGTCACTTACTCACAGACCTTCCTCTCCCTCCAGAGCTCCCTGGTGGAGATCTG; TCTCCCCCAGACTCTCCAGAACCAAAGGCAATCACACCACCTCAGCAACCATATAAAAAG; AGACCAAAAATTTGTTGTCCTCGTTATGGAGAAAGAAGACAAACAGAAAGCGACTGGGGG; AAACGCTGTGTGGACAAGTTTGACATTATTGGGATTATTGGAGAAGGAACCTATGGCCAA; GTATATAAAGCCAAGGACAAAGACACAGGAGAACTAGTGGCTCTGAAGAAGGTGAGACTA; GACAATGAGAAAGAGGGCTTCCCAATCACAGCCATTCGTGAAATCAAAATCCTTCGTCAG; TTAATCCACCGAAGTGTTGTTAACATGAAGGAAATTGTCACAGATAAACAAGATGCACTG; GATTTCAAGAAGGACAAAGGTGCCTTTTACCTTGTATTTGAGTATATGGACCATGACTTA; ATGGGACTGCTAGAATCTGGTTTGGTGCACTTTTCTGAGGACCATATCAAGTCGTTCATG; AAACAGCTAATGGAAGGATTGGAATACTGTCACAAAAAGAATTTCCTGCATCGGGATATT; AAGTGTTCTAACATTTTGCTGAATAACAGTGGGCAAATCAAACTAGCAGATTTTGGACTT; GCTCGGCTCTATAACTCTGAAGAGAGTCGCCCTTACACAAACAAAGTCATTACTTTGTGG; TACCGACCTCCAGAACTACTGCTAGGAGAGGAACGTTACACACCAGCCATAGATGTTTGG; AGCTGTGGATGTATTCTTGGGGAACTATTCACAAAGAAGCCTATTTTTCAAGCCAATCTG; GAACTGGCTCAGCTAGAACTGATCAGCCGACTTTGTGGTAGCCCTTGTCCAGCTGTGTGG; CCTGATGTTATCAAACTGCCCTACTTCAACACCATGAAACCGAAGAAGCAATATCGAAGG; CGTCTACGAGAAGAATTCTCTTTCATTCCTTCTGCAGCACTTGATTTATTGGACCACATG; CTGACACTAGATCCTAGTAAGCGGTGCACAGCTGAACAGACCCTACAGAGCGACTTCCTT; AAAGATGTCGAACTCAGCAAAATGGCTCCTCCAGACCTCCCCCACTGGCAGGATTGCCAT; GAGTTGTGGAGTAAGAAACGGCGACGTCAGCGACAAAGTGGTGTTGTAGTCGAAGAGCCA; CCTCCATCCAAAACTTCTCGAAAAGAAACTACCTCAGGGACAAGTACTGAGCCTGTGAAG; AACAGCAGCCCAGCACCACCTCAGCCTGCTCCTGGCAAGGTGGAGTCTGGGGCTGGGGAT; GCAATAGGCCTTGCTGACATCACACAACAGCTGAATCAAAGTGAATTGGCAGTGTTATTA; AACCTGCTGCAGAGCCAAACCGACCTGAGCATCCCTCAAATGGCACAGCTGCTTAACATC; CACTCCAACCCAGAGATGCAGCAGCAGCTGGAAGCCCTGAACCAATCCATCAGTGCCCTG; ACGGAAGCTACTTCCCAGCAGCAGGACTCAGAGACCATGGCCCCAGAGGAGTCTTTGAAG; GAAGCACCCTCTGCCCCAGTGATCCTGCCTTCAGCAGAACAGACGACCCTTGAAGCTTCA; AGCACACCAGCTGACATGCAGAATATATTGGCAGTTCTCTTGAGTCAGCTGATGAAAACC; CAAGAGCCAGCAGGCAGTCTGGAGGAAAACAACAGTGACAAGAACAGTGGGCCACAGGGG; CCCCGAAGAACTCCCACAATGCCACAGGAGGAGGCAGCAGCATGTCCTCCTCACATTCTT; CCACCAGAGAAGAGGCCCCCTGAGCCCCCCGGACCTCCACCGCCGCCACCTCCACCCCCT; CTGGTTGAAGGCGATCTTTCCAGCGCCCCCCAGGAGTTGAACCCAGCCGTGACAGCCGCC; TTGCTGCAACTTTTATCCCAGCCTGAAGCAGAGCCTCCTGGCCACCTGCCACATGAGCAC; CAGGCCTTGAGACCAATGGAGTACTCCACCCGACCCCGTCCAAACAGGACTTATGGAAAC; ACTGATGGGCCTGAAACAGGGTTCAGTGCCATTGACACTGATGAACGAAACTCTGGTCCA; GCCTTGACAGAATCCTTGGTCCAGACCCTGGTGAAGAACAGGACCTTCTCAGGCTCTCTG; AGCCACCTTGGGGAGTCCAGCAGTTACCAGGGCACAGGGTCAGTGCAGTTTCCAGGGGAC; CAGGACCTCCGTTTTGCCAGGGTCCCCTTAGCGTTACACCCGGTGGTCGGGCAACCATTC; CTGAAGGCTGAGGGAAGCAGCAATTCTGTGGTACATGCAGAGACCAAATTGCAAAACTAT; GGGGAGCTGGGGCCAGGAACCACTGGGGCCAGCAGCTCAGGAGCAGGCCTTCACTGGGGG; GGCCCAACTCAGTCTTCTGCTTATGGAAAACTCTATCGGGGGCCTACAAGAGTCCCACCA; AGAGGGGGAAGAGGGAGAGGAGTTCCTTACTAA;
• Restriction Sites: Please inquire
• ACCN: NM_016507
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_016507.1, NP_057591.1
• RefSeq Size: 5515 bp
• RefSeq ORF: 4473 bp
• Locus ID: 51755
• UniProt ID: Q9NYV4
• Cytogenetics: 17q12
• Domains: pkinase, S_TKc, TyrKc
• Protein Families: Druggable Genome, Protein Kinase
• Summary: Cyclin-dependent kinase that phosphorylates the C-terminal domain (CTD) of the large subunit of RNA polymerase II (POLR2A), thereby acting as a key regulator of transcription elongation. Regulates the expression of genes involved in DNA repair and is required for the maintenance of genomic stability. Preferentially phosphorylates 'Ser-5' in CTD repeats that are already phosphorylated at 'Ser-7', but can also phosphorylate 'Ser-2'. Required for RNA splicing, possibly by phosphorylating SRSF1/SF2. Involved in regulation of MAP kinase activity, possibly leading to affect the response to estrogen inhibitors.[UniProtKB/Swiss-Prot Function]; Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.