NCKAP1 (NM_205842) Human Untagged Clone

SKU SC308232

NCKAP1 (untagged)-Human NCK-associated protein 1 (NCKAP1), transcript variant 2

Product Data

• Type: Human Untagged Clone
• Target Symbol: NCKAP1
• Synonyms: HEM2; NAP1; NAP125; p125Nap1
• Vector: pCMV6 series
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_205842, the custom clone sequence may differ by one or more nucleotides; ATGTCGCGCTCAGTGCTGCAGCCCAGTCAGCAGAAGCTGGCGGAGAAGCTCACCATCCTC; AACGACCGGGGCGTCGGCATGCTCACCCGCCTCTACAACATCAAGAAGCAAGGACAAGTT; TGGAAGGCATGTGGAGACCCCAAGGCAAAACCATCCTATCTTATCGACAAAAACCTGGAA; TCTGCTGTGAAATTCATAGTCAGAAAATTCCCTGCTGTAGAAACCCGCAACAACAATCAA; CAGCTTGCACAACTACAGAAAGAAAAATCAGAGATTCTGAAAAATCTGGCATTATATTAC; TTCACATTTGTAGATGTTATGGAATTTAAGGACCATGTTTGTGAATTGCTGAATACTATT; GACGTTTGCCAAGTCTTCTTTGATATTACTGTAAACTTTGATTTAACAAAGAACTACTTA; GATTTAATTATAACCTATACAACACTAATGATACTGCTGTCTCGAATTGAAGAAAGGAAG; GCAATCATTGGATTATACAACTATGCCCATGAAATGACTCATGGAGCAAGTGACAGAGAA; TACCCACGCCTTGGCCAGATGATTGTGGATTATGAAAACCCTTTAAAGAAGATGATGGAA; GAATTTGTACCCCATAGCAAGTCTCTTTCAGATGCACTAATTTCTCTTCAAATGGTATAT; CCTCGAAGGAATCTTTCAGCTGACCAGTGGAGAAATGCCCAGTTATTGAGCCTCATCAGT; GCACCTAGTACAATGCTTAATCCAGCACAGTCCGACACTATGCCTTGTGAATACCTCTCT; TTGGATGCAATGGAAAAGTGGATTATCTTTGGCTTTATTTTGTGCCATGGGATCCTAAAT; ACTGACGCTACAGCACTGAACCTTTGGAAACTAGCTCTTCAAAGTAGCTCTTGCCTCTCT; CTCTTTCGGGATGAAGTTTTCCACATTCACAAAGCTGCAGAAGACTTATTTGTAAACATA; CGAGGCTATAATAAACGTATTAATGACATAAGAGAATGCAAGGAGGCAGCCGTGTCACAT; GCTGGTTCAATGCACAGAGAAAGACGCAAGTTTTTAAGATCTGCACTGAAGGAATTGGCT; ACTGTCCTCTCTGATCAACCTGGATTGCTAGGTCCCAAGGCACTTTTTGTTTTTATGGCA; TTATCCTTTGCCCGTGATGAAATCATCTGGCTACTTCGTCATGCAGATAACATGCCAAAG; AAGAGTGCAGACGACTTTATAGATAAGCACATTGCTGAATTAATATTTTACATGGAAGAA; CTTAGAGCACATGTGAGGAAATACGGACCTGTAATGCAGAGGTATTACGTGCAGTACCTT; TCTGGCTTTGATGCTGTTGTCCTCAATGAACTCGTGCAGAATCTTTCTGTTTGCCCTGAA; GATGAATCAATCATCATGTCCTCTTTTGTTAACACTATGACTTCCCTAAGTGTAAAACAA; GTTGAAGATGGGGAAGTATTTGATTTCAGAGGAATGAGATTAGATTGGTTTAGGTTACAG; GCATATACTAGTGTCTCAAAGGCTTCACTTGGCCTTGCAGATCACAGAGAACTTGGAAAG; ATGATGAATACAATAATTTTTCATACAAAAATGGTAGATTCCTTGGTGGAAATGTTGGTG; GAAACATCAGATCTCTCCATATTTTGTTTTTATAGTCGTGCTTTTGAGAAGATGTTTCAA; CAGTGTTTGGAGTTACCCTCTCAATCAAGATACTCAATTGCATTTCCACTACTTTGCACT; CATTTTATGAGTTGCACGCATGAACTATGTCCAGAAGAGCGACATCATATTGGAGATCGC; AGTCTTTCCTTATGTAATATGTTCCTAGATGAAATGGCCAAACAAGCTCGAAATCTCATC; ACTGATATTTGCACAGAACAGTGTACCCTTAGTGACCAGTTGCTACCCAAGCATTGTGCC; AAAACTATCAGTCAAGCAGTGAATAAGAAATCAAAAAAGCAGACTGGTAAGAAAGGGGAA; CCTGAAAGGGAGAAACCAGGTGTTGAGAGCATGAGGAAAAACAGGCTGGTTGTGACCAAC; CTTGATAAATTGCACACTGCACTTTCTGAGTTATGCTTCTCTATAAATTATGTACCAAAC; ATGGTGGTATGGGAACATACCTTTACCCCACGAGAATATTTGACTTCTCATCTGGAAATA; CGCTTTACCAAGTCAATTGTTGGGATGACTATGTATAATCAAGCCACACAGGAAATTGCA; AAACCTTCAGAACTTCTAACAAGTGTAAGAGCATACATGACCGTACTCCAGTCAATAGAA; AACTATGTGCAGATTGATATTACAAGAGTATTTAATAATGTGCTTCTTCAACAAACACAA; CATTTAGACAGTCATGGAGAGCCAACCATTACAAGTCTATACACAAATTGGTATTTGGAA; ACTTTGTTACGACAAGTCAGCAATGGCCATATAGCATATTTTCCTGCAATGAAAGCGTTT; GTGAACTTACCTACAGAAAATGAATTAACATTCAATGCAGAGGAATATTCTGACATATCA; GAAATGAGGTCATTATCAGAACTACTAGGCCCATATGGTATGAAGTTTCTAAGTGAAAGC; CTTATGTGGCATATTTCATCACAAGTTGCTGAACTTAAGAAACTTGTGGTGGAGAATGTT; GATGTGTTAACACAAATGAGGACCAGCTTTGACAAACCAGACCAGATGGCTGCACTGTTT; AAAAGATTATCATCTGTTGACAGTGTCTTGAAGAGGATGACAATAATTGGTGTAATTTTA; TCCTTCCGATCATTGGCACAAGAAGCACTTAGAGATGTCTTATCCTACCACATTCCTTTT; CTTGTAAGTTCAATTGAAGATTTTAAGGATCACATTCCAAGGGAAACTGATATGAAGGTT; GCAATGAATGTGTATGAGTTATCATCAGCTGCCGGATTACCTTGTGAGATTGATCCTGCA; TTGGTCGTAGCTCTTTCTTCACAAAAATCGGAAAACATTAGTCCAGAAGAAGAGTATAAA; ATTGCCTGCCTTCTCATGGTGTTTGTGGCAGTTTCTTTGCCAACACTGGCCAGTAATGTG; ATGTCTCAGTACAGCCCTGCTATAGAAGGGCATTGCAACAACATACATTGCTTGGCCAAA; GCCATCAACCAGATTGCTGCAGCTTTGTTTACAATTCACAAAGGAAGCATTGAAGACCGT; CTTAAAGAATTTCTGGCGCTTGCATCCTCCAGTCTACTGAAAATTGGCCAGGAGACAGAT; AAAACTACAACAAGAAATAGAGAATCTGTTTATTTACTGCTAGATATGATTGTACAAGAA; TCTCCATTCCTTACAATGGATCTTTTGGAATCTTGTTTTCCTTATGTCTTGCTGAGAAAT; GCATACCATGCTGTCTACAAACAAAGTGTTACATCTTCTGCATAA;
• Restriction Sites: Please inquire
• ACCN: NM_205842
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_205842.1, NP_995314.1
• RefSeq Size: 4637 bp
• RefSeq ORF: 3405 bp
• Locus ID: 10787
• UniProt ID: Q9Y2A7
• Cytogenetics: 2q32.1
• Protein Families: Druggable Genome
• Protein Pathways: Regulation of actin cytoskeleton
• Summary: Part of the WAVE complex that regulates lamellipodia formation. The WAVE complex regulates actin filament reorganization via its interaction with the Arp2/3 complex. Actin remodeling activity is regulated by RAC1. As component of the WAVE1 complex, required for BDNF-NTRK2 endocytic trafficking and signaling from early endosomes.[UniProtKB/Swiss-Prot Function]; Transcript Variant: This variant (2) contains an alternate in-frame exon in the 5' coding region, compared to variant 1. The encoded isoform (2) is shorter than isoform 1.