DOCK8 (NM_203447) Human Untagged Clone

CAT#: SC308184

DOCK8 (untagged)-Human dedicator of cytokinesis 8 (DOCK8), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DOCK8
• Synonyms: HEL-205; MRD2; ZIR8
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC308184 representing NM_203447.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGCCACTCTGCCGAGCGCAGAGCGCCGCGCGTTCGCGCTCAAGATCAACAGGTATTCTTCAGCGGAA
  ATAAGGAAACAGTTTACTCTCCCACCAAACCTTGGCCAGTACCATCGACAGAGCATAAGTACCTCTGGC
  TTCCCCTCTCTTCAACTACCTCAGTTTTATGACCCTGTGGAGCCAGTGGACTTTGAAGGACTTCTGATG
  ACACACCTGAACAGCCTGGATGTGCAGCTTGCCCAGGAGCTCGGGGACTTCACTGATGACGACTTGGAC
  GTGGTGTTCACGCCAAAGGAATGTAGGACTTTGCAGCCCTCTTTGCCGGAGGAAGGGGTTGAACTGGAC
  CCTCATGTCAGGGACTGTGTTCAGACCTACATCCGTGAGTGGCTAATCGTGAACCGGAAAAACCAAGGA
  AGTCCAGAAATCTGTGGCTTTAAAAAGACTGGATCTCGAAAAGATTTTCACAAGACGCTTCCGAAACAG
  ACGTTTGAGTCGGAAACCTTGGAGTGCAGTGAACCCGCTGCTCAGGCAGGCCCCCGCCACTTAAACGTG
  CTGTGCGACGTGTCTGGGAAAGGCCCCGTCACTGCCTGTGACTTTGACCTCCGCAGCCTGCAGCCTGAC
  AAGCGGCTAGAAAACCTCCTGCAGCAAGTGAGTGCCGAGGACTTTGAGAAGCAGAACGAGGAGGCCCGG
  AGGACCAATAGGCAGGCCGAGCTCTTTGCCCTTTACCCATCAGTGGACGAGGAGGATGCTGTGGAAATA
  CGTCCAGTACCAGAATGTCCCAAGGAACACCTGGGCAACAGAATATTGGTCAAGTTGCTGACCTTGAAG
  TTCGAGATTGAAATTGAGCCCCTGTTTGCCAGCATTGCCCTCTACGATGTTAAAGAAAGGAAAAAGATC
  TCAGAAAATTTTCACTGTGACCTGAACTCTGACCAGTTCAAAGGATTTCTGCGAGCTCACACGCCTTCA
  GTGGCCGCATCAAGTCAGGCGAGATCTGCAGTCTTCTCAGTCACCTACCCGTCCTCAGACATCTACCTG
  GTAGTCAAGATTGAAAAAGTCCTGCAGCAGGGAGAGATTGGAGACTGTGCAGAGCCCTACACGGTTATC
  AAAGAAAGTGATGGTGGAAAGAGTAAAGAAAAGATTGAAAAACTAAAACTCCAAGCTGAATCCTTCTGC
  CAGCGTTTGGGGAAATACCGGATGCCCTTTGCCTGGGCACCCATAAGCTTATCAAGCTTCTTCAATGTC
  TCCACCCTTGAGAGGGAGGTAACTGATGTGGACTCTGTGGTTGGGAGAAGCTCAGTGGGTGAACGGAGG
  ACATTGGCCCAATCTAGAAGGCTTTCTGAAAGAGCCCTCTCCTTGGAGGAAAATGGGGTTGGATCCAAC
  TTCAAAACCTCCACTCTGAGCGTTAGCAGCTTTTTCAAGCAGGAAGGAGATCGCCTTAGCGATGAAGAC
  TTATTCAAGTTTTTAGCTGACTACAAAAGATCATCATCCTTACAGAGACGAGTCAAGTCAATTCCAGGC
  TTGCTAAGACTGGAGATTTCTACAGCTCCAGAGATCATCAATTGCTGTCTGACTCCTGAAATGCTGCCC
  GTGAAACCCTTTCCTGAAAACCGGACACGCCCGCACAAAGAGATTTTGGAATTTCCAACACGAGAAGTA
  TATGTCCCTCACACTGTGTACAGAAACCTTCTCTATGTCTACCCACAGAGGCTGAACTTTGTAAACAAA
  CTAGCATCAGCCCGGAACATTACAATAAAGATCCAGTTTATGTGTGGAGAAGATGCTAGCAATGCGATG
  CCGGTCATCTTTGGAAAATCCAGCGGGCCTGAATTTCTGCAGGAAGTGTACACAGCTGTTACATACCAT
  AATAAGTCTCCTGACTTTTATGAAGAAGTGAAAATTAAGCTCCCCGCTAAGCTCACAGTAAATCACCAC
  CTCCTGTTCACCTTCTACCATATCAGCTGTCAGCAGAAGCAAGGAGCCTCCGTGGAAACTCTCCTGGGA
  TATTCATGGCTGCCAATTCTCTTAAATGAACGTCTTCAAACTGGATCCTACTGTCTCCCAGTTGCCTTG
  GAAAAATTGCCACCCAACTACTCCATGCATTCTGCTGAGAAAGTCCCATTACAGAATCCTCCCATTAAG
  TGGGCTGAAGGACATAAGGGAGTATTTAATATTGAAGTGCAAGCTGTTTCTTCTGTACACACCCAGGAC
  AACCACCTGGAGAAGTTCTTCACCCTCTGCCACTCCCTGGAGAGCCAGGTGACCTTCCCCATCCGCGTG
  CTGGATCAGAAAATCAGCGAGATGGCGCTGGAGCATGAGCTGAAGCTCAGCATCATCTGCCTGAACTCC
  TCCCGCCTGGAGCCGCTCGTGCTCTTCCTGCACCTGGTGCTGGACAAGCTCTTCCAGCTGTCCGTGCAG
  CCCATGGTCATCGCTGGCCAGACAGCCAACTTCTCCCAGTTTGCCTTCGAGTCCGTGGTGGCCATCGCC
  AACAGTCTGCACAACAGCAAGGACCTGAGCAAGGACCAGCATGGGAGGAACTGCCTGCTGGCTTCCTAC
  GTGCACTACGTCTTCCGCCTGCCAGAGGTGCAAAGGGATGTGCCCAAGTCAGGCGCTCCCACTGCCCTC
  CTAGACCCTCGGAGCTACCACACGTATGGCCGCACATCAGCTGCTGCTGTGAGTTCAAAGCTGCTGCAG
  GCCCGGGTGATGAGCAGCAGTAACCCAGACCTCGCGGGGACACACTCCGCAGCAGACGAGGAAGTGAAG
  AACATCATGTCTTCAAAGATCGCCGATCGCAACTGCAGCCGAATGTCTTACTATTGCTCTGGCAGTAGT
  GATGCTCCAAGTTCACCTGCAGCCCCAAGGCCAGCCAGCAAAAAGCATTTCCATGAGGAGCTTGCCCTT
  CAGATGGTGGTCAGCACCGGAATGGTGAGAGAAACAGTCTTCAAGTATGCCTGGTTCTTCTTTGAGCTT
  CTGGTGAAAAGCATGGCCCAGCACGTACATAACATGGACAAACGGGACAGTTTTCGGAGGACTCGTTTT
  TCTGACCGTTTCATGGATGACATAACTACTATTGTTAATGTGGTCACCTCGGAAATTGCAGCCCTTTTA
  GTAAAACCACAGAAGGAAAATGAACAGGCGGAAAAGATGAACATCAGCCTGGCTTTCTTCTTGTATGAC
  CTTCTCTCCCTCATGGATCGGGGCTTTGTGTTTAACCTCATCAGACATTATTGCAGCCAGCTGTCAGCC
  AAGCTCAGTAACCTTCCAACGCTCATTTCCATGAGGCTAGAGTTCCTGAGAATCCTCTGTAGCCATGAG
  CATTACCTCAATCTGAACCTTTTTTTTATGAATGCTGATACTGCTCCAACATCTCCTTGTCCTTCCATA
  TCTTCCCAGAACTCAAGCTCCTGCTCCAGCTTCCAGGACCAGAAGATCGCCAGCATGTTCGATCTGACT
  TCCGAGTACCGCCAGCAGCACTTCCTCACCGGGCTCCTCTTCACAGAACTGGCTGCTGCCCTGGATGCC
  GAAGGGGAAGGAATCAGCAAAGTACAAAGGAAAGCTGTCAGTGCAATTCACAGCCTGCTAAGTTCTCAC
  GACCTGGACCCACGCTGTGTCAAACCAGAGGTGAAGGTCAAAATCGCCGCCCTTTACCTACCTTTAGTT
  GGCATCATTTTGGATGCTTTGCCACAGCTCTGTGACTTTACAGTTGCAGATACTCGCAGATACCGCACC
  AGTGGCTCGGATGAAGAACAAGAAGGAGCCGGTGCCATTAACCAGAATGTGGCTCTGGCCATAGCAGGG
  AATAATTTCAATTTGAAAACAAGTGGAATAGTGCTGTCTTCCTTGCCCTATAAGCAGTACAACATGCTG
  AACGCGGACACTACTCGCAACCTCATGATCTGCTTCCTCTGGATCATGAAAAATGCTGATCAGAGCCTC
  ATTAGGAAGTGGATTGCTGACCTGCCATCAACGCAGCTCAACAGGATTTTAGATCTACTTTTCATCTGT
  GTGTTATGTTTTGAGTATAAGGGAAAACAGAGTTCTGACAAAGTCAGTACCCAAGTCCTGCAGAAGTCA
  AGGGATGTCAAGGCCCGGCTGGAAGAGGCTTTGCTCCGTGGGGAAGGGGCCAGAGGGGAGATGATGCGC
  CGCCGGGCTCCAGGGAACGACCGATTTCCAGGCCTAAATGAAAATTTGAGATGGAAGAAAGAGCAGACA
  CATTGGCGGCAAGCTAATGAGAAGCTAGATAAAACAAAGGCCGAGTTAGATCAAGAAGCCTTGATCAGT
  GGCAATCTGGCTACAGAAGCACATTTAATCATCCTGGATATGCAGGAAAACATTATCCAGGCGAGCTCG
  GCTCTGGACTGTAAAGACAGCCTGCTGGGAGGTGTTCTGAGGGTGCTGGTGAATTCTCTGAACTGTGAT
  CAGAGTACCACCTACCTGACTCACTGCTTTGCAACACTCCGTGCTCTCATCGCCAAGTTTGGAGACTTA
  CTCTTCGAAGAGGAGGTGGAACAGTGTTTCGACCTATGTCACCAAGTCCTGCACCACTGCAGCAGCAGC
  ATGGATGTCACCCGGAGCCAAGCCTGTGCCACCCTTTACCTCCTCATGAGGTTCAGTTTTGGAGCCACC
  AGTAATTTTGCAAGAGTAAAGATGCAAGTAACCATGTCCCTGGCATCTTTGGTGGGAAGAGCACCAGAC
  TTTAATGAAGAGCACCTGAGAAGATCCTTGAGGACAATTTTGGCCTATTCAGAAGAGGACACAGCCATG
  CAGATGACTCCTTTTCCCACCCAGGTGGAGGAACTTCTCTGTAATCTGAATAGCATCTTATATGACACA
  GTGAAAATGAGGGAATTTCAGGAAGATCCTGAGATGCTTATGGATCTCATGTACAGAATTGCCAAGAGT
  TACCAGGCATCTCCTGATCTGCGGCTGACCTGGCTCCAGAACATGGCAGAGAAACACACCAAGAAGAAG
  TGCTACACGGAGGCTGCCATGTGCCTGGTGCACGCCGCTGCGTTAGTGGCTGAGTATCTGAGCATGCTG
  GAGGACCACAGCTACCTGCCCGTGGGCAGTGTCAGCTTCCAGAATATTTCTTCCAATGTGCTGGAGGAG
  TCTGTGGTCTCTGAGGACACCCTGTCACCTGACGAGGATGGGGTGTGCGCAGGCCAGTACTTCACCGAG
  AGTGGCCTGGTAGGCCTCCTGGAGCAGGCCGCGGAGCTCTTCAGCACGGGAGGCTTATATGAGACAGTT
  AATGAGGTCTACAAGCTGGTCATCCCCATCCTAGAAGCGCATCGAGAATTCCGGAAGCTGACACTCACT
  CACAGCAAGCTGCAGAGAGCCTTCGACAGCATCGTTAACAAGGATCATAAGAGAATGTTTGGAACCTAC
  TTCCGAGTTGGTTTCTTTGGATCCAAATTTGGGGATTTGGATGAACAGGAGTTTGTCTACAAAGAGCCT
  GCAATTACCAAGCTTCCTGAGATCTCACATAGACTAGAGGCATTTTATGGTCAATGTTTTGGTGCAGAA
  TTTGTGGAAGTGATTAAAGACTCCACTCCTGTGGACAAAACCAAGTTGGATCCTAACAAGGCCTACATA
  CAGATCACTTTTGTGGAGCCCTACTTTGATGAGTATGAGATGAAAGACAGGGTCACATACTTTGAGAAG
  AATTTCAACCTCCGGAGGTTCATGTACACCACCCCGTTCACCCTGGAGGGGCGGCCTCGGGGAGAGCTG
  CATGAGCAGTACAGAAGGAACACAGTCCTGACCACTATGCACGCCTTCCCCTACATCAAGACCAGGATC
  AGCGTCATCCAGAAGGAGGAGTTTGTTTTGACACCGATTGAAGTTGCCATTGAAGACATGAAGAAGAAG
  ACCCTGCAGTTAGCAGTTGCCATTAACCAGGAGCCGCCTGATGCAAAGATGCTTCAGATGGTGCTGCAA
  GGCTCTGTGGGAGCTACTGTAAATCAGGGACCACTGGAAGTAGCCCAAGTGTTTTTGGCTGAAATTCCT
  GCTGATCCAAAACTCTATCGACATCACAACAAGTTGAGGTTATGCTTTAAGGAATTCATCATGAGATGT
  GGTGAAGCTGTAGAGAAAAACAAGCGTCTCATCACGGCAGACCAGAGGGAATATCAGCAGGAACTCAAA
  AAGAACTATAACAAGCTAAAAGAGAACCTCAGGCCAATGATCGAGCGGAAAATTCCAGAACTGTACAAG
  CCAATATTCAGAGTTGAGAGTCAAAAGAGGGACTCCTTCCACAGATCTAGTTTCAGGAAATGTGAAACC
  CAGTTGTCACAGGGCAGCTAA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_203447
• Insert Size: 6300 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_203447.3
• RefSeq Size: 7470 bp
• RefSeq ORF: 6300 bp
• Locus ID: 81704
• UniProt ID: Q8NF50
• Cytogenetics: 9p24.3
• MW: 238.5 kDa
• Gene Summary: This gene encodes a member of the DOCK180 family of guanine nucleotide exchange factors. Guanine nucleotide exchange factors interact with Rho GTPases and are components of intracellular signaling networks. Mutations in this gene result in the autosomal recessive form of the hyper-IgE syndrome. Alternatively spliced transcript variants encoding different isoforms have been described.[provided by RefSeq, Jun 2010]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).