INDOL1 (IDO2) (NM_194294) Human Untagged Clone

CAT#: SC307585

IDO2 (untagged)-Human indoleamine 2,3-dioxygenase 2 (IDO2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: INDOL1
• Synonyms: INDOL1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_194294 edited
  ATGTTGCATTTTCATTATTATGATACTTCAAACAAAATAATGGAGCCCCACAGACCGAAT
  GTGAAGACAGCAGTGCCATTGTCTTTGGAAAGCTATCACATATCTGAAGAGTATGGCTTT
  CTTCTTCCAGATTCTCTGAAAGAACTTCCAGATCATTATAGGCCTTGGATGGAAATTGCC
  AACAAACTTCCTCAATTGATTGATGCTCACCAGCTTCAAGCTCATGTGGACAAGATGCCC
  CTGCTGAGCTGCCAGTTCCTGAAGGGTCACCGGGAGCAGCGCCTGGCCCACCTGGTCCTG
  AGCTTCCTCACCATGGGTTATGTCTGGCAGGAAGGAGAGGCGCAGCCTGCAGAGGTCCTG
  CCAAGGAATCTTGCCCTTCCATTTGTCGAAGTCTCCAGGAACTTGGGGCTCCCTCCTATC
  CTGGTCCACTCAGACTTGGTGCTGACGAACTGGACCAAAAAAGATCCAGACGGATTCCTG
  GAAATTGGGAACCTGGAGACCATCATCTCATTTCCTGGGGGAGAGAGCCTGCATGGTTTT
  ATACTGGTGACTGCTTTGGTAGAGAAAGAAGCAGTGCCTGGGATAAAGGCTCTTGTTCAG
  GCCACGAATGCTATCTTGCAGCCCAACCAGGAGGCCCTGCTCCAAGCCCTGCAGCGACTG
  AGACTGTCTATTCAGGACATCACCAAAACCTTAGGACAGATGCATGATTATGTAGATCCA
  GACATATTTTATGCAGGCATCCGGATCTTTCTCTCTGGATGGAAAGACAACCCAGCAATG
  CCTGCAGGGCTGATGTATGAAGGAGTTTCCCAAGAGCCCCTGAAATACTCCGGCGGGAGT
  GCAGCTCAGAGCACAGTGCTTCATGCCTTTGATGAGTTCTTAGGCATTCGTCATAGCAAG
  GAAAGTGGTGACTTTCTGTACAGAATGAGGGATTACATGCCTCCTTCCCATAAGGCCTTC
  ATAGAAGACATCCACTCAGCACCTTCCCTGAGGGACTACATCCTGTCATCTGGACAGGAC
  CACTTGCTGACAGCTTATAACCAGTGTGTGCAGGCCCTGGCAGAGCTGCGGAGCTATCAC
  ATCACCATGGTCACCAAATACCTCATCACAGCTGCAGCCAAGGCAAAGCATGGGAAGCCA
  AACCATCTCCCAGGGCCTCCTCAGGCTTTAAAAGACAGGGGCACAGGTGGAACCGCAGTT
  ATGAGCTTTCTTAAGAGTGTCAGGGATAAGACCTTGGAGTCAATCCTTCACCCACGTGGT
  TAG
  
• Restriction Sites: Please inquire
• ACCN: NM_194294
• Insert Size: 1300 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_194294.1, NP_919270.1
• RefSeq Size: 3923 bp
• RefSeq ORF: 519 bp
• Locus ID: 169355
• UniProt ID: Q6ZQW0
• Cytogenetics: 8p11.21
• Protein Pathways: Metabolic pathways, Tryptophan metabolism
• Gene Summary: Along with the enzymes encoded by the INDO (MIM 147435) and TDO2 (MIM 191070) genes, the enzyme encoded by the INDOL1 gene metabolizes tryptophan in the kynurenine pathway (Ball et al., 2007 [PubMed 17499941]).[supplied by OMIM, Feb 2011]