Sphingosine 1 phosphate phosphatase 2 (SGPP2) (NM_152386) Human Untagged Clone

SKU SC306384

SGPP2 (untagged)-Human sphingosine-1-phosphate phosphatase 2 (SGPP2)

Product Data

• Type: Human Untagged Clone
• Target Symbol: Sphingosine 1 phosphate phosphatase 2
• Synonyms: SPP2; SPPase2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_152386 edited; ATGGCCGAGCTGCTGCGGAGCCTGCAGGATTCCCAGCTCGTCGCCCGCTTCCAGCGCCGC; TGCGGGCTCTTCCCCGCTCCGGATGAAGGCCCCCGGGAGAACGGCGCGGACCCCACGGAG; CGCGCGGCGCGGGTCCCCGGGGTCGAGCATCTCCCCGCAGCCAACGGCAAGGGCGGCGAG; GCTCCGGCCAACGGGCTGCGCAGAGCCGCGGCGCCGGAGGCTTATGTACAGAAGTACGTC; GTGAAGAATTATTTCTACTATTACCTATTCCAATTTTCAGCTGCTTTGGGCCAAGAAGTG; TTCTACATCACGTTTCTTCCATTCACTCACTGGAATATTGACCCTTATTTATCCAGAAGA; TTGATCATCATATGGGTTTTGGTGATGTATATTGGCCAAGTGGCCAAGGATGTCTTGAAG; TGGCCCCGTCCCTCCTCCCCTCCAGTTGTGAAACTGGAAAAGAGACTGATTGCTGAATAT; GGAATGCCATCCACCCACGCCATGGCGGCCACTGCCATTGCCTTCACCCTCCTTATCTCT; ACTATGGACAGATACCAGTATCCATTTGTGTTGGGACTGGTGATGGCCGTGGTGTTTTCC; ACCTTGGTGTGTCTCAGCAGGCTCTACACTGGGATGCATACGGTCCTGGATGTGCTGGGT; GGCGTCCTGATCACCGCACTCCTCATCGTCCTCACCTACCCTGCCTGGACCTTCATCGAC; TGCCTGGACTCGGCCAGCCCCCTCTTCCCCGTGTGTGTCATAGTTGTGCCATTCTTCCTG; TGTTACAATTACCCTGTTTCTGATTACTACAGCCCAACCCGGGCGGACACCACCACCATT; CTGGCTGCCGGGGCTGGAGTGACCATAGGATTCTGGATCAACCATTTCTTCCAGCTTGTA; TCCAAGCCCGCTGAATCTCTCCCTGTTATTCAGAACATCCCACCGCTCACCACCTACATG; TTAGTTTTGGGTCTGACCAAATTTGCAGTGGGAATTGTGTTGATCCTCTTGGTTCGTCAG; CTTGTACAAAATCTCTCACTGCAAGTATTATACTCATGGTTCAAGGTGGTCACCAGGAAC; AAGGAGGCCAGGCGGAGACTGGAGATTGAAGTGCCTTACAAGTTTGTTACCTACACATCT; GTTGGCATCTGCGCTACAACCTTTGTGCCGATGCTTCACAGGTTTCTGGGATTACCCTGA;
• Restriction Sites: Please inquire
• ACCN: NM_152386
• Insert Size: 1200 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The ORF of this clone has been fully sequenced and found to be a perfect match to NM_152386.2.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_152386.2, NP_689599.2
• RefSeq Size: 1200 bp
• RefSeq ORF: 1200 bp
• Locus ID: 130367
• UniProt ID: Q8IWX5
• Cytogenetics: 2q36.1
• Protein Families: Transmembrane
• Protein Pathways: Sphingolipid metabolism
• Summary: The protein encoded by this gene is a transmembrane protein that degrades the bioactive signaling molecule sphingosine 1-phosphate. The encoded protein is induced during inflammatory responses and has been shown to be downregulated by the microRNA-31 tumor suppressor. Alternative splice variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2016]; Transcript Variant: This variant (1) encodes the longer isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.