NALP4 (NLRP4) (NM_134444) Human Untagged Clone

SKU SC305988

NLRP4 (untagged)-Human NLR family, pyrin domain containing 4 (NLRP4)

Product Data

• Type: Human Untagged Clone
• Target Symbol: NALP4
• Synonyms: CLR19.5; CT58; NALP4; PAN2; PYPAF4; RNH2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_134444 edited; GGAGGAGCTGGAGAACATAGACAGGGATGAGGTTTTATTTATTTATTGTTCCTGGTCACT; GTCTCTTTGAGGATTGGTATCTCTGCTCCAGAAAAGATGGCAGCCTCTTTCTTCTCTGAT; TTTGGTCTTATGTGGTATCTGGAGGAGCTCAAAAAGGAGGAGTTCAGGAAATTTAAAGAA; CATCTCAAGCAAATGACTTTGCAGCTTGAACTCAAGCAGATTCCCTGGACTGAGGTCAAA; AAAGCATCCCGGGAAGAACTTGCAAACCTCTTGATCAAGCACTATGAAGAACAACAAGCT; TGGAACATAACCTTAAGAATCTTTCAAAAGATGGATAGAAAGGATCTCTGCATGAAGGTC; ATGAGGGAGAGAACAGGATACACAAAGACCTATCAAGCTCACGCAAAGCAGAAATTCAGC; CGCTTATGGTCCAGCAAGTCTGTCACTGAGATTCACCTATACTTTGAGGAGGAAGTCAAG; CAAGAAGAATGTGACCATTTGGACCGCCTTTTTGCTCCCAAGGAAGCTGGGAAACAGCCA; CGTACAGTGATCATTCAAGGACCACAAGGAATTGGAAAAACGACACTCCTGATGAAGCTG; ATGATGGCCTGGTCGGACAACAAGATCTTTCGGGATAGGTTCCTGTACACATTCTATTTC; TGCTGCAGAGAACTGAGGGAGTTGCCGCCAACGAGTTTGGCTGACTTGATTTCCAGAGAG; TGGCCTGACCCCGCTGCTCCTATAACAGAGATCGTGTCTCAACCGGAGAGACTCTTGTTC; GTCATCGACAGCTTCGAAGAGCTGCAGGGCGGCTTGAACGAACCCGATTCGGATCTGTGT; GGTGACTTGATGGAGAAACGGCCGGTGCAGGTGCTTCTGAGCAGTTTGCTGAGGAAGAAG; ATGCTCCCGGAGGCCTCCCTGCTCATCGCTATCAAACCCGTGTGCCCGAAGGAGCTCCGG; GATCAGGTGACGATCTCAGAAATCTACCAGCCCCGGGGATTCAACGAGAGTGATAGGTTA; GTGTATTTCTGCTGTTTCTTCAAAGACCCGAAAAGAGCCATGGAAGCCTTCAATCTTGTA; AGAGAAAGTGAACAGCTGTTTTCCATATGCCAAATCCCGCTCCTCTGCTGGATCCTGTGT; ACCAGTCTGAAGCAAGAGATGCAGAAAGGAAAAGACCTGGCCCTGACCTGCCAGAGCACT; ACCTCTGTGTACTCCTCTTTCGTCTTTAACCTGTTCACACCTGACGGTGCCGAGGGCCCG; ACTCAGCAAACCCAGCACCAGCTGAAGGCCCTGTGCTCCCTGGCTGCAGAGGGTATGTGG; ACAGACACATTTGAGTTTTGTGAAGACGACCTCCGGAGAAATGGGGTTGTTGACGCTGAC; ATCCCTGCGCTGCTGGGCACCAAGATACTTCTGAAGTACGGGGAGCGTGAGAGCTCCTAC; GTGTTCCTCCACGTGTGTATCCAGGAGTTCTGTGCCGCCTTGTTCTATTTGCTCAAGAGC; CACCTTGATCATCCTCACCCAGCTGTGAGATGTGTACAGGAATTGCTAGTTGCCAATTTT; GAAAAAGCAAGGAGAGCACATTGGATTTTTTTGGGGTGTTTTCTAACTGGCCTTTTAAAT; AAAAAGGAACAAGAAAAACTGGATGCGTTTTTTGGCTTCCAACTGTCCCAAGAGATAAAG; CAGCAAATTCACCAGTGCCTGAAGAGCTTAGGGGAGCGTGGCAATCCTCAGGGACAGGTG; GATTCCTTGGCGATATTTTACTGTCTCTTTGAAATGCAGGATCCTGCCTTTGTGAAGCAG; GCAGTGAACCTCCTCCAAGAAGCTAACTTTCATATTATTGACAACGTGGACTTGGTGGTT; TCTGCCTACTGCTTAAAATACTGCTCCAGCTTGAGGAAACTCTGTTTTTCCGTTCAAAAT; GTCTTTAAGAAAGAGGATGAACACAGCTCTACGTCGGATTACAGCCTCATCTGTTGGCAT; CACATCTGCTCTGTGCTCACCACCAGCGGGCACCTCAGAGAGCTCCAGGTGCAGGACAGC; ACCCTCAGCGAGTCGACCTTTGTGACCTGGTGTAACCAGCTGAGGCATCCCAGCTGTCGC; CTTCAGAAGCTTGGAATAAATAACGTTTCCTTTTCTGGCCAGAGTGTTCTGCTCTTTGAG; GTGCTCTTTTATCAGCCAGACTTGAAATACCTGAGCTTCACCCTCACGAAACTCTCTCGT; GATGACATCAGGTCCCTCTGTGATGCCTTGAACTACCCAGCAGGCAACGTCAAAGAGCTA; GCGCTGGTAAATTGTCACCTCTCACCCATTGATTGTGAAGTCCTTGCTGGCCTTCTAACC; AACAACAAGAAGCTGACGTATCTGAATGTATCCTGCAACCAGTTAGACACAGGCGTGCCC; CTTTTGTGTGAAGCCATGTGCAGCCCAGACACGGTCCTGGTATACCTGATGTTGGCTTTC; TGCCACCTCAGCGAGCAGTGCTGCGAATACATCTCTGAAATGCTTCTGCGTAACAAGAGC; GTGCGCTATCTAGACCTCAGTGCCAATGTCCTGAAGGACGAAGGACTGAAAACTCTCTGC; GAGGCCTTGAAACATCCGGACTGCTGCCTGGATTCACTGTGTTTGGTAAAATGTTTTATC; ACTGCTGCTGGCTGTGAAGACCTCGCCTCTGCTCTCATCAGCAATCAAAACCTGAAGATT; CTGCAAATTGGGTGCAATGAAATCGGAGATGTGGGTGTGCAGCTGTTGTGTCGGGCTCTG; ACGCATACGGATTGCCGCTTAGAGATTCTTGGGTTGGAAGAATGTGGGTTAACGAGCACC; TGCTGTAAGGATCTCGCGTCTGTTCTCACCTGCAGTAAGACCCTGCAGCTGCTCAACCTG; ACCTTGAACACCTTGGACCACACAGGGGTGGTTGTACTCTGTGAGGCCCTGAGACACCCA; GAGTGTGCCCTGCAGGTGCTCGGGCTGAGAAAAACTGATTTTGATGAGGAAACCCAGGCA; CTGCTGACGGCTGAGGAAGAGAGAAATCCTAACCTGACCATCACAGACGACTGTGACACA; ATCACAAGGGTAGAGATCTGATTGCGAGGAACCTGGGCTCTGACTCGAACACCTGCAAAG; GACAGGGACTGGGACCGTTACTTACATGACACTGCACCCAGGAGATACAAATCATTGACA; CTCTGAGTTGTGAGATTTCTGGCACCCCATTCATAGATTTGATATGATACACGTGGTTTT; TATGTGCTCTGTGGCCTTGGATGAGTCACTGAAAGGCCTTCATGGTCTCTCGGTCTCACA; AGGACCTCTTAACCCCTCAATAAAGTGTTACATTTCTAAACATTAAAAGAAAAAAAAAAA; AAAAA;
• Restriction Sites: Please inquire
• ACCN: NM_134444
• Insert Size: 3400 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The ORF of this clone has been fully sequenced and found to contain 4 SNPs compared with reference sequence NM_134444.3.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_134444.3, NP_604393.1
• RefSeq Size: 3339 bp
• RefSeq ORF: 2985 bp
• Locus ID: 147945
• UniProt ID: Q96MN2
• Cytogenetics: 19q13.43
• Summary: The protein encoded by this gene is a member of the nucleotide-binding and leucine-rich repeat receptor (NLR) family, and is predicted to contain an N-terminal pyrin effector domain (PYD), a centrally-located nucleotide-binding and oligomerization domain (NACHT) and C-terminal leucine-rich repeats (LRR). This gene product has a demonstrated role as a negative regulator of autophagy and type I interferon signaling pathways as a result of protein interactions with its NACHT domain. The PYD domain has also been shown to be important in the inhibition of NF-kB (nuclear factor kappa-light-chain-enhancer of activated B cells). [provided by RefSeq, Dec 2016]