Menin (MEN1) (NM_130802) Human Untagged Clone

CAT#: SC305910

MEN1 (untagged)-Human multiple endocrine neoplasia I (MEN1), transcript variant e1D

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MEN1
• Synonyms: MEAI; SCG2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC305910 representing NM_130802.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGGGCTGAAGGCCGCCCAGAAGACGCTGTTCCCGCTGCGCTCCATCGACGACGTGGTGCGCCTGTTT
  GCTGCCGAGCTGGGCCGAGAGGAGCCGGACCTGGTGCTCCTTTCCTTGGTGCTGGGCTTCGTGGAGCAT
  TTTCTGGCTGTCAACCGCGTCATCCCTACCAACGTTCCCGAGCTCACCTTCCAGCCCAGCCCCGCCCCC
  GACCCGCCTGGCGGCCTCACCTACTTTCCCGTGGCCGACCTGTCTATCATCGCCGCCCTCTATGCCCGC
  TTCACCGCCCAGATCCGAGGCGCCGTCGACCTGTCCCTCTATCCTCGAGAAGGGGGTGTCTCCAGCCGT
  GAGCTGGTGAAGAAGGTCTCCGATGTCATATGGAACAGCCTCAGCCGCTCCTACTTCAAGGATCGGGCC
  CACATCCAGTCCCTCTTCAGCTTCATCACAGGTTGGAGCCCAGTAGGCACCAAATTGGACAGCTCCGGT
  GTGGCCTTTGCTGTGGTTGGGGCCTGCCAGGCCCTGGGTCTCCGGGATGTCCACCTCGCCCTGTCTGAG
  GATCATGCCTGGGTAGTGTTTGGGCCCAATGGGGAGCAGACAGCTGAGGTCACCTGGCACGGCAAGGGC
  AACGAGGACCGCAGGGGCCAGACAGTCAATGCCGGTGTGGCTGAGCGGAGCTGGCTGTACCTGAAAGGA
  TCATACATGCGCTGTGACCGCAAGATGGAGGTGGCGTTCATGGTGTGTGCCATCAACCCTTCCATTGAC
  CTGCACACCGACTCGCTGGAGCTTCTGCAGCTGCAGCAGAAGCTGCTCTGGCTGCTCTATGACCTGGGA
  CATCTGGAAAGGTACCCCATGGCCTTAGGGAACCTGGCAGATCTAGAGGAGCTGGAGCCCACCCCTGGC
  CGGCCAGACCCACTCACCCTCTACCACAAGGGCATTGCCTCAGCCAAGACCTACTATCGGGATGAACAC
  ATCTACCCCTACATGTACCTGGCTGGCTACCACTGTCGCAACCGCAATGTGCGGGAAGCCCTGCAGGCC
  TGGGCGGACACGGCCACTGTCATCCAGGACTACAACTACTGCCGGGAAGACGAGGAGATCTACAAGGAG
  TTCTTTGAAGTAGCCAATGATGTCATCCCCAACCTGCTGAAGGAGGCAGCCAGCTTGCTGGAGGCGGGC
  GAGGAGCGGCCGGGGGAGCAAAGCCAGGGCACCCAGAGCCAAGGTTCCGCCCTCCAGGACCCTGAGTGC
  TTCGCCCACCTGCTGCGATTCTACGACGGCATCTGCAAATGGGAGGAGGGCAGTCCCACGCCTGTGCTG
  CACGTGGGCTGGGCCACCTTTCTTGTGCAGTCCCTAGGCCGTTTTGAGGGACAGGTGCGGCAGAAGGTG
  CGCATAGTGAGCCGAGAGGCCGAGGCGGCCGAGGCCGAGGAGCCGTGGGGCGAGGAAGCCCGGGAAGGC
  CGGCGGCGGGGCCCACGGCGGGAGTCCAAGCCAGAGGAGCCCCCGCCGCCCAAGAAGCCAGCACTGGAC
  AAGGGCCTGGGCACCGGCCAGGGTGCAGTGTCAGGACCCCCCCGGAAGCCTCCTGGGACTGTCGCTGGC
  ACAGCCCGAGGCCCTGAAGGTGGCAGCACGGCTCAGGTGCCAGCACCCGCAGCATCACCACCGCCGGAG
  GGTCCAGTGCTCACTTTCCAGAGTGAGAAGATGAAGGGCATGAAGGAGCTGCTGGTGGCCACCAAGATC
  AACTCGAGCGCCATCAAGCTGCAACTCACGGCACAGTCGCAAGTGCAGATGAAGAAGCAGAAAGTGTCC
  ACCCCTAGTGACTACACTCTGTCTTTCCTCAAGCGGCAGCGCAAAGGCCTCTGA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_130802
• Insert Size: 1848 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_130802.2
• RefSeq Size: 3172 bp
• RefSeq ORF: 1848 bp
• Locus ID: 4221
• UniProt ID: O00255
• Cytogenetics: 11q13.1
• Protein Families: Druggable Genome, Transcription Factors
• MW: 68 kDa
• Gene Summary: This gene encodes menin, a tumor suppressor associated with a syndrome known as multiple endocrine neoplasia type 1. Menin is a scaffold protein that functions in histone modification and epigenetic gene regulation. It is thought to regulate several pathways and processes by altering chromatin structure through the modification of histones. [provided by RefSeq, May 2019]
  Transcript Variant: This variant (e1D) has a unique 5' UTR different from that of variant 1. Variants 1, e1B, e1C, e1D, e1E, and e1F1 all encode the same isoform (1).