MADCAM1 (NM_130762) Human Untagged Clone

SKU SC305896

MADCAM1 (untagged)-Human mucosal vascular addressin cell adhesion molecule 1 (MADCAM1), transcript variant 2

Product Data

• Type: Human Untagged Clone
• Target Symbol: MADCAM1
• Synonyms: MACAM1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_130762 edited; GGCGGCCGCGAATTCATGGATTTCGGACTGGCCCTCCTGCTGGCGGGGCTTCTGGGGCTC; CTCCTCGGCCAGTCCCTCCAGGTGAAGCCCCTGCAGGTGGAGCCCCCGGAGCCGGTGGTG; GCCGTGGCCTTGGGCGCCTCGCGCCAGCTCACCTGCCGCCTGGCCTGCGCGGACCGCGGG; GCCTCGGTGCAGTGGCGGGGCCTGGACACCAGCCTGGGCGCGGTGCAGTCGGACACGGGC; CGCAGCGTCCTCACCGTGCGCAACGCCTCGCTGTCGGCGGCCGGGACCCGCGTGTGCGTG; GGCTCCTGCGGGGGCCGCACCTTCCAGCACACCGTGCAGCTCCTTGTGTACGCCTTCCCG; GACCAGCTGACCGTCTCCCCGGCAGCCCTGGTGCCTGGTGACCCGGAGGTGGCCTGTACG; GCCCACAAAGTCACGCCCGTGGACCCCAACGCGCTCTCCTTCTCCCTGCTCGTCGGGGGC; CAGGAACTGGAGGGGGCGCAAGCCCTGGGCCCGGAGGTGCAGGAGGAGGAGGAGGAGCCC; CAGGGGGACGAGGACGTGCTGTTCAGGGTGACAGAGCGCTGGCGGCTGCCGCCCCTGGGG; ACCCCTGTCCCGCCCGCCCTCTACTGCCAGGCCACGATGAGGCTGCCTGGCTTGGAGCTC; AGCCACCGCCAGGCCATCCCCGCGTCCAAACCTGCGGGTGACCAGCTGCCCGCGGCTCTG; TGGACCAGCAGTGCGGTGCTGGGACTGCTGCTCCTGGCCTTGCCCACCTATCACCTCTGG; AAACGCTGCCGGCACCTGGCTGAGGACGACACCCACCCACCGGCTTCTCTGAGGCTTCTG; CCCCAGGTGTCGGCCTGGGCTGGGTTAAGGGGGACCGGCCAGGTCGGGATCAGCCCCTCC; TGAGTGGCCAGCCTTTCCCCCTGTGAAAGCAAAATAGCTTGGACCCCTTCAAGTTGAGAA; CTGGTCAGGGCAAACCTGCCTCCCATTCTACTCAAAGTCATCCCTCTGCTCACAGAGATG; GATGCATGTTCTGATTGCCTCTTTGGAGAAGCTCATCAGAAACTCAAAAGAAGGCCACTG; TTTGTCTCACCTACCCATGACCTGAAGCCCCTCCCTGAGTGGTCCCCACCTTTCTGGACG; GAACCACGTACTTTTTACATACATTGATTCATGTCTCACGTCTCCCTAAAAATGCGTAAG; ACCAAGCTGTGCCCTGACCACCCTGGGCCCCTGTCGTCAGGACCTCCTGAGGCTTTGGCA; AATAAACCTCCTAAAATGATAAAAAAAAAAAAAAAAAAACTCGAC;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_130762 unedited; NGGGGAGTTCAAATTTGTATACGACTCATATAGGCGGCCGCGATTCATGGATTTCGGACT; GGCCCTCCTGCTGGCGGGGCTTCTGGGGCTCCTCCTCGGCCAGTCCCTCCAGGTGAAGCC; CCTGCAGGTGGAGCCCCCGGAGCCGGTGGTGGCCGTGGCCTTGGGCGCCTCGCGCCAGCT; CACCTGCCGCCTGGCCTGCGCGGACCGCGGGGCCTCGGTGCAGTGGCGGGGCCTGGACAC; CAGCCTGGGCGCGGTGCAGTCGGACACGGGCCGCAGCGTCCTCACCGTGCGCAACGCCTC; GCTGTCGGCGGCCGGGACCCGCGTGTGCGTGGGCTCCTGCGGGGGCCGCACCTTCCAGCA; CACCGTGCAGCTCCTTGTGTACGCCTTCCCGGACCAGCTGACCGTCTCCCCGGCAGCCCT; GGTGCCTGGTGACCCGGAGGTGGCCTGTACGGCCCACAAAGTCACGCCCGTGGACCCCAA; CGCGCTCTCCTTCTCCCTGCTCGTCGGGGGCCAGGAACTGGAGGGGGCGCAAGCCCTGGG; CCCGGAGGTGCANGAGGAGGAGGAGGAGCCCCAGGGGGACGAGGACGTGCTGTTCAGGGT; GACAGAGCGCTGGCGGCTGCCGCCCCTGGGGACCCCTGTCCCGCCCGCCCTCTACTGCCA; GGCCACGATGAGGCTGCCTGGCTTGGAGCTCAGCCACCGCCAGGCCATCCCCGCGTCCAA; ACCTGCGGGTGACCAGCTGCCCGCGGCTCTGTGGACCAGCAGTGCGGTGCTGGGACTGCT; GCTCCTGGCCTTGCCCACCTATCACCTCTGGAAACGCTGCCGGCACCTGGCTNGAGACGA; CACCCACCCC;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_130762 unedited; CATTGGNGAATGGCACTTNCCAGGNCCAGGNGAAAGCACTGGGNGNAGGGGTCACAGGGN; ATGCCACCCGGGNNATCTGTTCAGGNAAACAGCTATGACCGCGGCCGCAATCTAGAGTCG; AGTTTTTTTTTTTTTTTTTTTATCATTTTAGGAGGTTTATTTGCCAAAGCCTCAGGAGGT; CCTGACGACAGGGGCCCAGGGTGGTCAGGGCACAGCTTGGTCTTACGCATTTTTAGGGAG; ACGTGAGACATGAATCAATGTATGTAAAAAGTACGTGGTTCCGTCCAGAAAGGTGGGGAC; CACTCAGGGAGGGGCTTCAGGTCATGGGTAGGTGAGACAAACAGTGGCCTTCTTTTGAGT; TTCTGATGAGCTTCTCCAAAGAGGCAATCAGAACATGCATCCATCTCTGTGAGCAGAGGG; ATGACTTTGAGTAGAATGGGAGGCAGGTTTGCCCTGACCAGTTCTCAACTTGAAGGGGTC; CAAGCTATTTTGCTTTCACAGGGGGAAAGGCTGGCCACTCAGGAGGGGCTGATCCCGACC; TGGCCGGTCCCCCTTAACCCAGCCCAGGCCGACACCTGNGGCAGAAGCCTCAGAAAGCCG; GTGGGTTGGGTGTCGTCCTCAGCCAGGTGCCGGCAGCGTTTCCAGAGGTGATAGGTGGGC; AAGGCCAGGAGCAGCAGTCCCAGCACCGCACTGCTGGTCCACAGAGCCGCGGGCAGCTGG; TCACCCCGCAGTTTGGACGCGGGGATGGCTTGGCGGGGGGTGAGCTCCAGCCCAGCAGCC; TCATCGTGGCCTTGCAATAAAGGGCGGGCGGGAAAGGGGGTCCCCAGGGGGCGGAGCCGC; C;
• Restriction Sites: Please inquire
• ACCN: NM_130762
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_130762.1, NP_570118.1
• RefSeq Size: 1275 bp
• RefSeq ORF: 888 bp
• Locus ID: 8174
• UniProt ID: Q13477
• Cytogenetics: 19p13.3
• Protein Pathways: Cell adhesion molecules (CAMs)
• Summary: The protein encoded by this gene is an endothelial cell adhesion molecule that interacts preferentially with the leukocyte beta7 integrin LPAM-1 (alpha4beta7), L-selectin, and VLA-4 (alpha4beta1) on myeloid cells to direct leukocytes into mucosal and inflamed tissues. It is a member of the immunoglobulin family and is similar to ICAM1 and VCAM1. At least seven alternatively spliced transcripts encoding different protein isoforms have been found for this gene, but the full-length nature of some variants has not been determined. [provided by RefSeq, Jul 2008]; Transcript Variant: This variant (2) lacks an alternate in-frame exon, compared to variant 1, resulting in a shorter protein (isoform b) that lacks an internal segment, compared to isoform a.