TBX1 (NM_080647) Human Untagged Clone

SKU SC305810

TBX1 (untagged)-Human T-box 1 (TBX1), transcript variant C

Product Data

• Type: Human Untagged Clone
• Target Symbol: TBX1
• Synonyms: CAFS; CATCH22; CTHM; DGCR; DGS; DORV; TBX1C; TGA; VCF; VCFS
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_080647 edited; ATGCACTTCAGCACCGTCACCAGGGACATGGAAGCCTTCACGGCCAGCAGCCTGAGCAGC; CTGGGGGCCGCGGGGGGCTTCCCGGGCGCCGCGTCGCCCGGCGCCGACCCGTACGGCCCG; CGCGAGCCCCCGCCGCCGCCGCGCTACGACCCGTGCGCCGCCGCCGCCCCCGGCGCCCCG; GGTCCGCCGCCGCCGCCGCACGCCTACCCGTTTGCGCCGGCCGCCGGGGCCGCCACCAGC; GCCGCCGCCGAGCCCGAGGGCCCCGGGGCCAGCTGCGCGGCCGCAGCCAAGGCGCCGGTG; AAGAAGAACGCGAAGGTGGCCGGTGTGAGCGTGCAGCTAGAGATGAAGGCGCTGTGGGAC; GAGTTCAACCAGCTGGGCACCGAGATGATCGTCACCAAGGCCGGCAGGCGGATGTTTCCC; ACCTTCCAAGTGAAGCTCTTCGGCATGGATCCCATGGCCGACTATATGCTGCTCATGGAC; TTCGTGCCGGTGGACGATAAGCGCTACCGGTACGCCTTCCACAGCTCCTCCTGGCTGGTG; GCGGGGAAGGCCGACCCTGCCACGCCAGGCCGCGTGCACTACCACCCGGACTCGCCTGCC; AAGGGCGCGCAGTGGATGAAGCAAATCGTGTCCTTCGACAAGCTCAAGCTGACCAACAAC; TTACTGGACGACAACGGCCACATTATTCTGAATTCCATGCACAGATACCAGCCCCGCTTC; CACGTGGTCTATGTGGACCCACGCAAAGATAGCGAGAAATATGCCGAGGAGAACTTCAAA; ACCTTTGTGTTCGAGGAGACACGATTCACCGCGGTCACTGCCTACCAGAACCATCGGATC; ACGCAGCTCAAGATTGCCAGCAATCCCTTCGCGAAAGGCTTCCGGGACTGTGACCCTGAG; GACTGGCCCCGGAACCACCGGCCCGGCGCACTGCCGCTCATGAGCGCCTTCGCGCGCTCG; CGGAACCCCGTGGCTTCCCCGACGCAGCCCAGCGGCACGGAGAAAGACGCGGCTGAGGCC; CGGCGAGAATTCCAGCGCGACGCGGGCGGGCCAGCGGTGCTCGGGGACCCGGCGCATCCT; CCGCAGCTGCTGGCCCGGGTGCTAAGCCCCTCGCTGCCCGGGGCCGGCGGCGCCGGCGGC; TTAGTCCCGCTGCCCGGCGCGCCCGGAGGCCGGCCCAGTCCCCCGAACCCCGAGCTGCGC; CTGGAGGCGCCCGGCGCATCGGAGCCGCTGCACCACCACCCCTACAAATATCCGGCCGCC; GCCTACGACCACTATCTCGGGGCCAAGAGCCGGCCGGCGCCCTACCCGCTGCCCGGCCTG; CGTGGCCACGGCTACCACCCGCACGGGCATCCGCACCACCACCACCACCCCGTGAGTCCA; GCCGCCGCGGCCGCCGCCGCCGCTGCCGCAGCTGCCGCGGCCGCCAACATGTACTCGTCG; GCCGGAGCCGCGCCGCCCGGCTCCTACGACTATTGCCCCAGATAACACGGGCCCTGTCGC; GCTCCCGCCCCGGTCCTGCACAGCCCCGAAGTTCGCCGGGCCCGGCCACCCTGCCCCAAG; GGCAAGCAAGGAATACGTTCCCCCAGCCCCAGGGGCCACCGCGGCTCTCCCCTTCCCCAG; CCTCGAAGCCATGGGGGCCCCCTCGCCACCCCCAGCCCCTTGGGCTATCGAAGTATCCGG; TTCCCCAGTCCCTGGAGCCACCGCGGGTCCTTCCCCGGCCCCGAGGGCCAAGGGGGTCCC; CGCCCGCCAGTGCCAAAGCGCCCGGTCGGAGGCGGAAGGAAGTGATATTTATTGTTCTCC; CCGAGACCGCGTCGCCCGCGGCCCGGCCGGCAGTTGCAGTGTAGACAGCCCGAGAGCCCC; GCCTGCAGGCGGTGTAGATACATGTAGATACTGTAGATACTGTAGATACCGCCCCGGCGC; CGACTTGATAAACGGTTTCGCCTCTTTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_080647 unedited; GGGTTACCATTTGNTAATACGACTCACTATAGGGCGGCCGCGATTCAGATCTGGTACCAT; GCACTTCAGCACCGTCACCAGGGACATGGAAGCCTTCACGGCCAGCAGCCTGAGCAGCCT; GGGGGCCGCGGGGGGCTTCCCGGGCGCCGCGTCGCCCGGCGCCGACCCGTACGGCCCGCG; CGAGCCCCCGCCGCCGCCGCGCTACGACCCGTGCGCCGCCGCCGCCCCCGGCGCCCCGGG; TCCGCCGCCGCCGCCGCACGCCTACCCGTTTGCGCCGGCCGCCGGGGCCGCCACCAGCGC; CGCCGCCGAGCCCGAGGGCCCCGGGGCCAGCTGCGCGGCCGCAGCCAAGGCGCCGGTGAA; GAAGAACGCGAAGGTGGCCGGTGTGAGCGTGCAGCTAGAGATGAAGGCGCTGTGGGACGA; GTTCAACCAGCTGGGCACCGAGATGATCGTCACCAAGGCCGGCAGGCGGATGTTTCCCAC; CTTCCAAGTGAAGCTCTTCGGCATGGATCCCATGGCCGACTATATGCTGCTCATGGACTT; CGTGCCGGTGGACGATAAGCGCTACCGGTCCCCCTCCACAGCTCCTCCTGGCTGGTGGCG; GGAATGCCGACCCTGGCACGCCAGGCCCGGTGCCCTACCACCCGGACTTCGCTGGCAAGG; GCGCGCATGGGATGAAGCAAATGTGGTCTTCACAAGCTCAAGCGGACCACAACCTTATGG; ACAACAACGGCCACATATCTCGAATTCAAGCCCAATTCCCACCCCCTTTCCCGTGGTCTA; TGGGGACCCCCCAAAATAGCCAGAATATGCCGAGGAGAAATTAAACCCTTTGTCTCAAGG; AAACCATTTAACCGGGGACTGGCCTCCAAAACCTTGGATTCCCCCCCTTAATTGGCCGCA; ATCC;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_080647 unedited; ACATGGNGATGGCACTTCAGGCCAGGAGGCACTGGGGAGGGTCACAGGGTGCCACCCGGG; ATCTGTTAGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTT; TTTTTTTTTTTTTCCAAAAGAGGCGAAACCGTTTATCAAGTCGGCGCCGGGGCGGTATCT; ACAGTATCTACAGTATCTACATGTATCTACACCGCCTGCAGGCGGGGCTCTCGGGCTGTC; TACACTGCAACTGCCGGCCGGGCCGCGGGCGACGCGGTCTCGGGGAGAACAATAAATATC; ACTTCCTTCCGCCTCCGACCGGGCGCTTTGGCACTGGCGGGCGGGGACCCCCTTGGCCCT; CGGGGCCGGGGAAGGACCCGCGGTGGCTCCAGGGACTGGGGAACCGGATACTTCGATAGC; CCAAGGGGCTGGGGGTGGCGAGGGGGCCCCCATGGCTTCNAGGCTGGGGAAGGGGAGAGC; CGCGGTGGCCCCTGGGGCTGGGGGAACGTATTCCTTGCTTGCCCTTGGGGCAGGGTGGCC; GGGCCCGGCGAACTTCGGGGCTGTGCAGGACCGGGGCGGGAGCGCGACAGGGCCCGTGTT; ATCTGGGGCAATAGTCGTAGGAGCCGGGCGGCGCGGCTCCGGCCGACGAGTACATGTTGG; CGGCCGCGGCAGCTGCGGCAGCGGCGGCGGCGGCCGCGGCGGCTGGACTCACGGNGTGGT; GGTGGTGGTGCGGATGCCCGTGCGGGTGGTAGCCGTGGCCACGCAGGCCGGGCAGCGGGT; AGGGCGCCCGCCCGCTCTTTGCCCCAAGATAGTGGTCGTAGGCCGCCGCCGGATTTTTTA; GGGGTGGTGGTGCAACGGCTTCCATTGCCCGGGCGGCTCCAGCCCCACTTGGGGTTCCGG; GGACTGGGCCGG;
• Restriction Sites: Please inquire
• ACCN: NM_080647
• Insert Size: 2000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: There is 2 nucleotide difference between the OriGene clone and the NCBI reference ORF. These result in the substitution of 1 aa and deletion of 1 aa.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_080647.1, NP_542378.1
• RefSeq Size: 2082 bp
• RefSeq ORF: 1488 bp
• Locus ID: 6899
• UniProt ID: O43435
• Cytogenetics: 22q11.21
• Protein Families: Transcription Factors
• Summary: This gene is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. This gene product shares 98% amino acid sequence identity with the mouse ortholog. DiGeorge syndrome (DGS)/velocardiofacial syndrome (VCFS), a common congenital disorder characterized by neural-crest-related developmental defects, has been associated with deletions of chromosome 22q11.2, where this gene has been mapped. Studies using mouse models of DiGeorge syndrome suggest a major role for this gene in the molecular etiology of DGS/VCFS. Several alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]; Transcript Variant: This variant (C) encodes the longest isoform (C) with the same N-terminal 336 aa, but an unique C-terminus with respect to isoforms A and B.