Lipin 3 (LPIN3) (NM_022896) Human Untagged Clone

SKU SC305060

LPIN3 (untagged)-Human lipin 3 (LPIN3)

Product Data

• Type: Human Untagged Clone
• Target Symbol: Lipin 3
• Synonyms: dJ620E11.2; LIPN3L; SMP2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_022896 edited; GGCGGCCGCGAATTCATGAACTACGTGGGGCAGCTGGCGGAGACGGTGTTTGGGACGGTG; AAGGAGCTGTACCGGGGCCTGAACCCAGCCACACTGAGCGGCGGCATTGACGTGCTGGTG; GTGAAGCAGGTGGACGGCTCGTTCCGGTGCTCACCCTTCCACGTGCGTTTTGGCAAGCTG; GGCGTCCTGCGGTCGCGGGAGAAGGTGGTAGACATTGAGCTCAATGGGGAGCCTGTGGAC; TTGCACATGAAGCTTGGGGACAGCGGGGAGGCCTTCTTTGTTCAGGAGCTGGAGAGCGAT; GATGAACATGTGCCTCCCGGCCTGTGCACCTCACCCATCCCTTGGGGGGGTCTGTCTGGC; TTCCCCTCGGACTCCCAGCTGGGCACTGCCAGTGAGCCTGAGGGCCTCGTCATGGCAGGC; ACGGCCTCCACTGGGCGGAGGAAGAGGCGTCGCAGGAGGAAACCCAAGCAGAAAGAGGAT; GCAGTGGCAACTGATTCTAGTCCAGAGGAACTGGAGGCAGGCGCTGAGAGTGAGCTATCC; CTGCCGGAAAAGCTGAGGCCAGAGCCCCCAGGTGTCCAGTTGGAAGAGAAGTCTTCACTG; CAGCCCAAAGACATCTACCCCTACTCGGATGGCGAGTGGCCCCCCCAGGCCAGCCTCTCA; GCAGGTGAGCTAACATCCCCTAAGAGCGACTCGGAGCTGGAGGTGCGGACCCCGGAGCCC; AGTCCCCTAAGAGCCGAGTCCCACATGCAGTGGGCCTGGGGGAGGCTGCCTAAGGTGGCC; AGAGCTGAGCGGCCCGAGTCCTCAGTGGTCCTTGAAGGCAGAGCTGGGGCAACCTCTCCT; CCTCGGGGAGGACCCAGCACTCCCTCTACCTCTGTGGCTGGCGGCGTGGACCCTTTGGGA; CTCCCAATCCAGCAAACAGAGGCTGGTGCCGACCTTCAGCCTGACACAGAGGATCCCACT; CTAGTGGGTCCCCCTCTCCACACCCCAGAGACAGAGGAAAGCAAGACTCAGAGCTCTGGG; GACATGGGCCTCCCTCCTGCCTCCAAGTCATGGAGCTGGGCCACTCTGGAGGTTCCAGTT; CCCACCGGGCAGCCAGAGAGGGTCTCCAGGGGGAAAGGCTCCCCAAAGAGAAGCCAGCAC; CTGGGCCCCAGTGACATCTACCTGGATGACTTGCCCTCCCTGGACTCTGAGAATGCAGCG; CTTTACTTCCCCCAAAGTGACTCTGGGCTGGGGGCCAGAAGATGGAGTGAACCCAGCAGT; CAGAAGTCCCTGAGGGACCCCAACCCTGAACATGAACCTGAACCCACTCTGGACACAGTG; GATACAATAGCACTGTCCCTCTGTGGTGGACTGGCTGACAGCCGGGACATCTCCCTAGAG; AAATTCAACCAGCACAGCGTCTCTTACCAGGACCTCACCAAAAACCCCGGACTTTTGGAT; GACCCAAACCTAGTGGTGAAAATCAATGGAAAGCATTATAACTGGGCTGTGGCTGCCCCC; ATGATCCTCTCCCTGCAAGCCTTCCAGAAAAACTTGCCCAAGAGCACCATGGACAAGCTG; GAGAGGGAGAAGATGCCCCGGAAGGGTGGGCGATGGTGGTTTTCCTGGCGACGCAGGGAC; TTCCTGGCCGAGGAGCGCAGTGCCCAGAAGGAGAAGACTGCAGCCAAGGAGCAGCAGGGG; GAGAAGACAGAAGTCCTGAGCAGTGATGACGATGCCCCAGACAGCCCTGTGATCCTGGAG; ATCCCCTCCTTGCCACCCTCCACTCCACCCTCCACTCCTACCTACAAGAAGTCCCTCCGC; CTCTCCTCCGATCAGATCCGGCGCCTGAACCTGCAAGAAGGTGCCAATGATGTGGTCTTC; AGCGTGACCACTCAGTACCAGGGCACCTGCCGCTGCAAGGCCACCATCTACCTGTGGAAA; TGGGACGACAAGGTGGTCATCTCTGACATCGACGGCACCATCACCAAGTCAGATGCTCTG; GGCCATATCCTGCCCCAGCTGGGGAAAGACTGGACACACCAGGGCATCACCAGTCTCTAT; CACAAAATCCAACTAAATGGGTACAAGTTCCTGTACTGCTCGGCGCGGGCCATTGGCATG; GCGGACCTCACCAAGGGGTACCTGCAGTGGGTGAGCGAGGGGGGCTGTAGCCTCCCCAAG; GGCCCCATCCTTCTGTCTCCCAGCAGCCTCTTCTCTGCCCTCCACAGAGAGGTGATCGAG; AAGAAACCAGAGGTGTTCAAGGTCGCCTGCCTGAGTGACATCCAGCAGCTGTTTCTGCCC; CACGGACAGCCCTTCTATGCTGCCTTTGGGAATAGGCCCAATGATGTCTTTGCCTACCGG; CAGGTGGGCCTGCCTGAGTCACGCATCTTCACAGTCAACCCCCGGGGAGAGCTCATCCAG; GAGCTCATAAAGAACCACAAATCCACGTATGAGCGGCTTGGTGAAGTGGTCGAGCTCCTC; TTCCCACCTGTGGCCCGTGGCCCCAGCACAGACCTGGCCAACCCTGAATACAGTAACTTC; TGCTACTGGCGGGAGCCACTGCCTGCTGTGGACCTTGATACCCTGGACTGAGTCGACTGC; AGAGGCCTGCATGCAAGC;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_022896 unedited; NGAGGTCAAATTTTGTATACGACTCATATAGGCGGCCGCGNATTCATGAACTACGTGGGG; CAGCTGGCGGAGACGGTGTTTGGGACGGTGAAGGAGCTGTACCGGGGCCTGAACCCAGCC; ACACTGAGCGGCGGCATTGACGTGCTGGTGGTGAAGCAGGTGGACGGCTCGTTCCGGTGC; TCACCCTTCCACGTGCGTTTTGGCAAGCTGGGCGTCCTGCGGTCGCGGGAGAAGGTGGTA; GACATTGAGCTCAATGGGGAGCCTGTGGACTTGCACATGAAGCTTGGGGACAGCGGGGAG; GCCTTCTTTGTTCAGGAGCTGGAGAGCGATGATGAACATGTGCCTCCCGGCCTGTGCACC; TCACCCATCCCTTGGGGGGGTCTGTCTGGCTTCCCCTCGGACTCCCAGCTGGGCACTGCC; AGTGAGCCTGAGGGCCTCGTCATGGCAGGCACGGCCTCCACTGGGCGGAGGAAGAGGCGT; CGCAGGAGGAAACCCAAGCAGAAAGAGGATGCAGTGGCAACTGATTCTAGTCCAGAGGAA; CTGGAGGCAGGCGCTGAGAGTGAGCTATCCCTGCCGGANAAGCTGAGGCCAGAGCCCCCA; GGTGTCCAGTTGGAAGAGAAGTCTTCACTGCAGCCCANAGACATCTACCCCTACTCGGAT; GGCGAGTGGCCCCCCCAGGCCAGCCTCTCAGCAGGTGAGCTAACATCCCCTAAGAGCGAC; TCGGAGCTGGAGGTGCGGACCCCGGAGCCCAGTCCCCTAAGAGCCGAGTCCCACATGCAG; TGGGCCTGGGGGAGGCTGCCCTAAGTGGCCAGAGCTGAGCGGCCCGAGTCCTCAGTGGTC; CTTGAAGGCAGAGCTGGNGGCACCTCTCCTCCTCCGGGGAGACCCAGCACTCCCTT;
• Restriction Sites: Please inquire
• ACCN: NM_022896
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_022896.1, NP_075047.1
• RefSeq Size: 4464 bp
• RefSeq ORF: 2556 bp
• Locus ID: 64900
• Cytogenetics: 20q12
• Summary: The protein encoded by this gene is a member of the lipin family of proteins, and all family members share strong homology in their C-terminal region. This protein is thought to form hetero-oligomers with other lipin family members, while one family member, lipin 1, can also form homo-oligomers. This protein contains conserved motifs for phosphatidate phosphatase 1 (PAP1) activity as well as a domain that interacts with a transcriptional co-activator. Lipin complexes act in the cytoplasm to catalyze the dephosphorylation of phosphatidic acid to produce diacylglycerol, which is the precursor of both triglycerides and phospholipids. Lipin complexes are also thought to regulate gene expression as transcriptional co-activators in the nucleus. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2014]