FGF21 (NM_019113) Human Untagged Clone

SKU SC304675

FGF21 (untagged)-Human fibroblast growth factor 21 (FGF21)

Product Data

• Type: Human Untagged Clone
• Target Symbol: FGF21
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_019113 edited; GAGGCTTCCAAGGCAGGATACTTGTGTCTCAGATGCGGTCGCTTCTTTCATACAGCAATT; GCCGCCTTGCTGAGGATCAAGGAACCTCAGTGTCAGATCACGCCCTCCCCCCAAACTTAG; AAATTCAGATGGGGCGCAGAAATTTCTCTTGTTCTGCGTGATCTGCATAGATGGTCCAAG; AGGTGGTTTTTCCAGGAGCCCAGCACCCCTCCTCCCTCCGACTCAGACCCAGGAGTCTGG; CCCTCCATTGAAAGGACCCCAGGTTACATCATCCATTCAGGCTGCCCTTGCCACGATGGA; ATTCTGTAGCTCCTGCCAAATGGGTCAAATATCATGGTTCAGGCGCAGGGAGGGTGATTG; GGCGGGCCTGTCTGGGTATAAATTCTGGAGCTTCTGCATCTATCCCAAAAAACAAGGGTG; TTCTGTCAGCTGAGGATCCAGCCGAAAGAGGAGCCAGGCACTCAGGCCACCTGAGTCTAC; TCACCTGGACAACTGGAATCTGGCACCAATTCTAAACCACTCAGCTTCTCCGAGCTCACA; CCCCGGAGATCACCTGAGGACCCGAGCCATTGATGGACTCGGACGAGACCGGGTTCGAGC; ACTCAGGGCTGTGGGTTTCTGTGCTGGCTGGTCTTCTGCTGGGAGCCTGCCAGGCACACC; CCATCCCTGACTCCAGTCCTCTCCTGCAATTCGGGGGCCAAGTCCGGCAGCGGTACCTCT; ACACAGATGATGCCCAGCAGACAGAAGCCCACCTGGAGATCAGGGAGGATGGGACGGTGG; GGGGCGCTGCTGACCAGAGCCCCGAAAGTCTCCTGCAGCTGAAAGCCTTGAAGCCGGGAG; TTATTCAAATCTTGGGAGTCAAGACATCCAGGTTCCTGTGCCAGCGGCCAGATGGGGCCC; TGTATGGATCGCTCCACTTTGACCCTGAGGCCTGCAGCTTCCGGGAGCTGCTTCTTGAGG; ACGGATACAATGTTTACCAGTCCGAAGCCCACGGCCTCCCGCTGCACCTGCCAGGGAACA; AGTCCCCACACCGGGACCCTGCACCCCGAGGACCAGCTCGCTTCCTGCCACTACCAGGCC; TGCCCCCCGCACCCCCGGAGCCACCCGGAATCCTGGCCCCCCAGCCCCCCGATGTGGGCT; CCTCGGACCCTCTGAGCATGGTGGGACCTTCCCAGGGCCGAAGCCCCAGCTACGCTTCCT; GAAGCCAGAGGCTGTTTACTATGACATCTCCTCTTTATTTATTAGGTTATTTATCTTATT; TATTTTTTTATTTTTCTTACTTGAGATAATAAAGAGTTCTAGAGGAGGATAAAAAAAAAA; AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_019113 unedited; NNGGGTGGCGTACGAATTTTGTAATACGACTCACTATAGGGCGGACCGCGATTCAGATCT; GGTACCGGTCCGGAATTCCCGGGATATCGTCGACCCACGCGTCCGCCCACGCGTCCGGAG; GCTTCCAAGGCAGGATACTTGTGTCTCAGATGCGGTCGCTTCTTTCATACAGCAATTGCC; GCCTTGCTGAGGATCAAGGAACCTCAGTGTCAGATCACGCCCTCCCCCCAAACTTAGAAA; TTCAGATGGGGCGCAGAAATTTCTCTTGTTCTGCGTGATCTGCATAGATGGTCCAAGAGG; TGGTTTTTCCAGGAGCCCAGCACCCCTCCTCCCTCCGACTCAGACCCAGGAGTCTGGCCC; TCCATTGAAAGGACCCCAGGTTACATCATCCATTCAGGCTGCCCTTGCCACGATGGAATT; CTGTAGCTCCTGCCAAATGGGTCAAATATCATGGTTCAGGCGCAGGGAGGGTGATTGGGC; GGGCCTGTCTGGGTATAAATTCTGGAGCTTCTGCATCTATCCCAAAAAACAAGGGTGTTC; TGTCAGCTGAGGATCCAGCCGAAAGAGGAGCCAGGCACTCAGGCCACCTGAGTCTACTCA; CCTGGACAACTGGAATCTGGCACCAATTCTAAACCACTCAGCTTCTCCGAGCTCACACCC; CGGAGATCACCTGAGGACCCGAGCCATTGATGGACTCGGACGAGACCGGGTTCGAGCACT; CAGGGCTGTGGGTTTCTGTGCTGGCTGGTCTTCTGCTGGGAGCCTGCCAGGCACACCCCA; TCCCTGACTCCAGTCCTCTCCTGGCATTCGGGGGCCAAGTCCGGCAGCGGTACCTCTACA; CAGATGATGCCCAGCAGACAGAAGCCACCTGGNAATCAGGGAGATGGGACNGTNGGGGGG; CGCTGCTGAC;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_019113 unedited; AGAGCCATTGGGGGAATGGGTTCACCAGGAATCCACCCGGGCATTTTTTCAGGAAAAGCT; TATGACCGCGGCCGCCCTAATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT; TTTTTTTTTTTTTTTTTTTAAACCCCCTCTAAAACTTTTTATTATCTCAAGTTAGAAAAA; TAAAAAAATAAATAAGATAAATAACCTAATAAATAAAGAGGAGATGTCATAGTAAACAGC; CTTTGGCTTCAAGAAACGTAACTGGGGCTTCGGCCCTGGGAAGGTCCCACCATGCTCAGA; GGGTCCGAGGAGCCCACATCGGGGGGCTGGGGGGCCAGGATTCCGGGTGGCTCCGGGGGT; GCGGGGGGCAGGCCTGGTAGTGGCAGGAAGCGAGCTGGTCCTCGGGGTGCAGGGTCCCGG; TGTGGGGACTTTGTCCCTGGCAGGTGCAACGGGAGGCCGTGGGCTTCGGACTGGTAAACA; TTGTATCCGTCCTCAAGAAGCAGCTTCCGGAAGCTGCAGGCCTTCAGGTCAAAGTGGAGC; GATTCATACAGGGCCCCATCTGGGCCCTGGCACAGGAACCCTGAAGTTTTTGACTCCCAA; GATATTGATAACTCCCGGCTTCAAAGCTTTTAACCTGCAGGAAACTTTTGGGGGCTTTTG; GTAANAAACGCCCCCCACCGTCCCATTCCTCCCTGATTTCCAGGTGGGCTTTCTGTCTGG; CTGGGCATCATCTTGTTTAAAGGTCCCGCTTGCCCGGAATTGGGCCCCCAAAATGCAAGG; AAAAGACTTGGAGTCACGGATTGGGGTGTTGCCTTGGAAAGCCTCCCCAGCAAAAAACCC; GCCCGCACAAGAACCCCCAACGCCTTGATGGCTTTAACCCGGTTTTTTTTCC;
• Restriction Sites: NotI-NotI
• ACCN: NM_019113
• Insert Size: 1400 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.; ; The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_019113.2, NP_061986.1
• RefSeq Size: 940 bp
• RefSeq ORF: 630 bp
• Locus ID: 26291
• UniProt ID: Q9NSA1
• Cytogenetics: 19q13.33
• Protein Families: Adult stem cells, Embryonic stem cells, ES Cell Differentiation/IPS, Secreted Protein
• Protein Pathways: MAPK signaling pathway, Melanoma, Pathways in cancer, Regulation of actin cytoskeleton
• Summary: Theis gene encodes a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities and are involved in a variety of biological processes. This protein is a secreted endocrine factor that functions as a major metabolic regulator. The encoded protein stimulates the uptake of glucose in adipose tissue. [provided by RefSeq, Mar 2016]