ANKHD1 (NM_017978) Human Untagged Clone

SKU SC304551

ANKHD1 (untagged)-Human ankyrin repeat and KH domain containing 1 (ANKHD1), transcript variant 2

Product Data

• Type: Human Untagged Clone
• Target Symbol: ANKHD1
• Synonyms: MASK; MASK1; PP2500; VBARP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC304551 representing NM_017978.; Blue=Insert sequence Red=Cloning site Green=Tag(s); ; GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG; GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC; ATGCTGACTGATAGCGGAGGCGGCGGCACCTCCTTTGAGGAGGACCTGGACTCTGTGGCTCCGCGATCC; GCCCCAGCTGGGGCCTCGGAGCCGCCTCCGCCGGGAGGGGTCGGTCTGGGGATCCGCACCGTGAGGCTC; TTTGGGGAGGCCGGGCCAGCGTCGGGAGTCGGCAGCAGCGGCGGCGGCGGCAGCGGCAGCGGTACGGGC; GGAGGGGACGCGGCGCTGGATTTCAAGTTGGCGGCTGCCGTGCTGAGGACCGGGGGTGGAGGTGGTGCC; TCTGGCAGTGACGAGGACGAAGTGTCCGAGGTTGAATCATTTATTTTGGACCAAGAAGATCTGGATAAC; CCAGTGCTTAAAACAACATCAGAGATATTCTTATCAAGTACTGCAGAAGGAGCAGACTTACGCACTGTG; GATCCAGAGACACAGGCACGACTAGAAGCATTGCTAGAAGCAGCAGCTTTTGCAGATCCTGAGGTACTC; CGGAGACTGACATCCTCAGTTAGTTGTGCACTGGATGAAGCTGCTGCTGCACTGACACGGATGAAAGCA; GAAAACAGCCACAATGCAGGACAAGTGGACACTCGCAGTCTAGCAGAAGCTTGTTCAGATGGGGATGTT; AATGCTGTTCGTAAATTGCTAGATGAAGGCAGAAGTGTAAATGAACATACAGAAGAAGGAGAAAGCCTG; CTGTGTTTGGCTTGTTCAGCAGGGTATTATGAATTAGCACAAGTATTGCTTGCTATGCATGCTAATGTT; GAAGATCGAGGGAATAAAGGAGACATAACTCCCCTGATGGCAGCTTCCAGTGGAGGTTACTTAGATATT; GTGAAATTATTACTTCTTCATGATGCTGATGTCAACTCCCAGTCTGCAACAGGAAACACTGCGCTAACT; TATGCATGTGCTGGAGGATTTGTTGACATTGTTAAAGTGCTCCTTAATGAAGGTGCAAATATAGAAGAT; CATAATGAAAATGGACATACTCCCTTAATGGAAGCAGCCAGTGCAGGTCATGTGGAAGTTGCAAGAGTT; CTTTTAGATCATGGTGCAGGCATCAACACTCATTCTAATGAATTCAAAGAAAGTGCTCTAACACTTGCT; TGCTACAAAGGCCATTTGGATATGGTTCGCTTTCTACTTGAAGCTGGTGCAGATCAAGAGCACAAAACA; GATGAGATGCACACTGCCTTAATGGAGGCCTGCATGGATGGACATGTAGAGGTGGCACGTTTGCTTTTG; GATAGTGGTGCTCAAGTGAACATGCCTGCAGATTCATTTGAATCTCCATTGACGCTAGCTGCCTGTGGA; GGACATGTTGAATTGGCAGCTCTACTTATTGAAAGGGGAGCAAATCTTGAAGAAGTTAATGATGAAGGA; TACACTCCCTTGATGGAAGCTGCCCGGGAAGGACATGAAGAAATGGTGGCACTACTCTTAGCACAAGGA; GCAAATATAAATGCCCAGACAGAAGAAACTCAAGAAACTGCTCTTACTTTGGCTTGCTGTGGAGGATTT; TCTGAAGTTGCAGACTTTCTTATTAAGGCAGGGGCTGATATAGAACTTGGCTGCTCCACACCTCTGATG; GAGGCATCTCAGGAGGGACACCTGGAATTGGTTAAATATTTGCTGGCTTCTGGCGCTAATGTGCATGCT; ACAACAGCAACAGGAGACACAGCCTTAACCTATGCTTGTGAAAATGGACATACGGATGTTGCAGATGTT; TTACTTCAAGCAGGGGCTGATTTAGACAAGCAGGAGGACATGAAGACTATTTTGGAGGGCATAGATCCG; GCCAAGCATCAGGTGAGGGTGGCCTTTGATGCTTGTAAGCTACTACGTAAAGAATAG; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT; TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC;
• Restriction Sites: SgfI-MluI
• ACCN: NM_017978
• Insert Size: 1851 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_017978.2
• RefSeq Size: 2161 bp
• RefSeq ORF: 1851 bp
• Locus ID: 54882
• UniProt ID: Q8IWZ3
• Cytogenetics: 5q31.3
• MW: 63.9 kDa
• Summary: This gene encodes a protein with multiple ankyrin repeat domains and a single KH-domain. The protein is thought to function as a scaffolding protein, and it may be involved in the regulation of caspases and thereby play an antiapoptotic role in cell survival. Alternative splicing results in multiple transcript variants, one of which generates a fusion transcript (MASK-BP3) with the downstream eIF4E-binding protein 3 (EIF4EBP3) gene, resulting in a protein comprised of the ANKHD1 sequence for the majority of the protein and a different C-terminus due to an alternate reading frame for the EIF4EBP3 segments. [provided by RefSeq, Sep 2010]; Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' coding region, lacks several exons in the central and 3' coding regions, and uses an alternate 3' terminal exon, compared to variant 1. The resulting isoform (2) is significantly shorter and has a distinct C-terminus, compared to isoform 1.