AMBN (NM_016519) Human Untagged Clone

CAT#: SC304427

AMBN (untagged)-Human ameloblastin (enamel matrix protein) (AMBN)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: AMBN
• Synonyms: AI1F
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_016519 edited
  CTTGGTTGGCATCATCAGGCCCTGAGAGCACAGTGCATGTCAGCATCTAAGATTCCACTT
  TTCAAAATGAAGGACCTGATACTGATCCTATGCCTCCTGGAAATGAGTTTTGCAGTGCCG
  TTCTTTCCTCAGCAATCTGGAACACCGGGTATGGCTAGTTTGAGCCTTGAGACAATGAGA
  CAGTTGGGAAGTCTGCAGAGATTAAACACACTTTCTCAGTATTCTAGATACGGCTTTGGA
  AAATCATTTAATTCTTTGTGGATGCACGGTCTCCTCCCACCACATTCCTCTCTTCCATGG
  ATGAGGCCAAGAGAACATGAAACTCAACAGTATGAATATTCTTTGCCTGTGCATCCCCCA
  CCTCTCCCATCACAGCCATCCTTGAAGCCTCAACAGCCAGGACTGAAACCTTTTCTCCAG
  TCTGCTGCTGCAACCACCAACCAGGCCACAGCACTGAAAGAAGCACTTCAGCCTCCAATT
  CACCTGGGACATCTGCCCTTGCAGGAAGGAGAACTGCCTCTGGTTCAGCAGCAGGTGGCA
  CCATCAGATAAGCCACCAAAGCCTGAGCTCCCAGGAGTAGATTTTGCTGATCCACAAGGT
  CCATCACTCCCAGGAATGGATTTTCCTGATCCACAAGGTCCATCACTCCCAGGATTGGAT
  TTTGCTGATCCACAAGG:TTCAACAATTTTCCAAATAGCCCGTTTGATTTCTCACGGACC
  AATGCCACAAAATAAACAATCTCCACTTTATCCAGGAATGTTGTACGTGCCTTTTGGAGC
  AAATCAATTGAATGCCCCTGCCAGACTTGGCATCATGAGTTCAGAAGAAGTGGCAGGCGG
  GAGAGAAGACCCAATGGCCTATGGAGCCATGTTTCCAGGATTTGGAGGCATGAGGCCCGG
  CTTTGAGGGAATGCCCCACAACCCAGCTATGGGCGGTGACTTCACTCTGGAATTTGACTC
  CCCAGTGGCTGCCACCAAAGGCCCTGAGAACGAAGAAGGAGGTGCACAAGGCTCCCCTAT
  GCCGGAGGCCAACCCAGACAATCTAGAAAACCCAGCTTTCCTTACAGAGCTAGAACCTGC
  TCCCCACGCAGGGCTCCCTGCTCTCCCTAAGGATGACATTCCCGGCCTGCCAAGGAGCCC
  TTCAGGGAAGATGAAGGGACTCCCCAGCGTCACCCCAGCAGCTGCTGACCCACTGATGAC
  CCCTGAATTAGCTGATGTTTATAGGACCTACGATGCTGACATGACCACATCCGTGGATTT
  CCAGGAAGAAGCAACCATGGATACCACGATGGCCCCAAACTCTCTGCAAACATCCATGCC
  AGGAAACAAAGCCCAGGAGCCCGAGATGATGCATGACGCATGGCATTTCCAAGAGCCCTG
  ACAGCTCTAAGATATTAGCTACTTTCTGTATGCACAAGCTTCCCAGCTTTGTCCCCACAG
  TGTACCTTTTTGCTAAAACACTTATTACCCTTCTGCAGCAAAGGCATTAAAAGCGCTAAG
  CATATATTAATAAATGCAAGTGGCTAGAAATAGTGTAGGTCCCCTTCTTGCTTTCAATAT
  CTTGTTGAAATAAAATGTGTCAATTGTCTCTGTGATTTAGAAACACTATTAATAACATCA
  GAGCAAGGTTCTAAGGGTCTCAGCATTTGATCATCACTTTTTCTTAGCTGTCTTAAGCAT
  TATAGAATTTCTCTTACCAGCATGACACTATTATATTCAGGAAACATGGCACTGCTTTTT
  TCTCTAAGCAAAGGCAAATATCCTCATAATTCTAAGCTAATTCATTTAAACTTTATTAAA
  TGGGGATTGGTGGAAAACTCCTGACTGGT
  
• Restriction Sites: Please inquire
• ACCN: NM_016519
• Insert Size: 1900 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The ORF of this clone has been fully sequenced and found to contain one SNP compared with NM_016519.4.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016519.4, NP_057603.1
• RefSeq Size: 1999 bp
• RefSeq ORF: 1344 bp
• Locus ID: 258
• UniProt ID: Q9NP70
• Cytogenetics: 4q13.3
• Protein Families: Secreted Protein
• Gene Summary: This gene encodes the nonamelogenin enamel matrix protein ameloblastin. The encoded protein may be important in enamel matrix formation and mineralization. This gene is located in the calcium-binding phosphoprotein gene cluster on chromosome 4. Mutations in this gene may be associated with dentinogenesis imperfect and autosomal dominant amylogenesis imperfect. [provided by RefSeq, Aug 2011]