AMBN (NM_016519) Human Untagged Clone

SKU SC304427

AMBN (untagged)-Human ameloblastin (enamel matrix protein) (AMBN)

Product Data

• Type: Human Untagged Clone
• Target Symbol: AMBN
• Synonyms: AI1F
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_016519 edited; CTTGGTTGGCATCATCAGGCCCTGAGAGCACAGTGCATGTCAGCATCTAAGATTCCACTT; TTCAAAATGAAGGACCTGATACTGATCCTATGCCTCCTGGAAATGAGTTTTGCAGTGCCG; TTCTTTCCTCAGCAATCTGGAACACCGGGTATGGCTAGTTTGAGCCTTGAGACAATGAGA; CAGTTGGGAAGTCTGCAGAGATTAAACACACTTTCTCAGTATTCTAGATACGGCTTTGGA; AAATCATTTAATTCTTTGTGGATGCACGGTCTCCTCCCACCACATTCCTCTCTTCCATGG; ATGAGGCCAAGAGAACATGAAACTCAACAGTATGAATATTCTTTGCCTGTGCATCCCCCA; CCTCTCCCATCACAGCCATCCTTGAAGCCTCAACAGCCAGGACTGAAACCTTTTCTCCAG; TCTGCTGCTGCAACCACCAACCAGGCCACAGCACTGAAAGAAGCACTTCAGCCTCCAATT; CACCTGGGACATCTGCCCTTGCAGGAAGGAGAACTGCCTCTGGTTCAGCAGCAGGTGGCA; CCATCAGATAAGCCACCAAAGCCTGAGCTCCCAGGAGTAGATTTTGCTGATCCACAAGGT; CCATCACTCCCAGGAATGGATTTTCCTGATCCACAAGGTCCATCACTCCCAGGATTGGAT; TTTGCTGATCCACAAGG:TTCAACAATTTTCCAAATAGCCCGTTTGATTTCTCACGGACC; AATGCCACAAAATAAACAATCTCCACTTTATCCAGGAATGTTGTACGTGCCTTTTGGAGC; AAATCAATTGAATGCCCCTGCCAGACTTGGCATCATGAGTTCAGAAGAAGTGGCAGGCGG; GAGAGAAGACCCAATGGCCTATGGAGCCATGTTTCCAGGATTTGGAGGCATGAGGCCCGG; CTTTGAGGGAATGCCCCACAACCCAGCTATGGGCGGTGACTTCACTCTGGAATTTGACTC; CCCAGTGGCTGCCACCAAAGGCCCTGAGAACGAAGAAGGAGGTGCACAAGGCTCCCCTAT; GCCGGAGGCCAACCCAGACAATCTAGAAAACCCAGCTTTCCTTACAGAGCTAGAACCTGC; TCCCCACGCAGGGCTCCCTGCTCTCCCTAAGGATGACATTCCCGGCCTGCCAAGGAGCCC; TTCAGGGAAGATGAAGGGACTCCCCAGCGTCACCCCAGCAGCTGCTGACCCACTGATGAC; CCCTGAATTAGCTGATGTTTATAGGACCTACGATGCTGACATGACCACATCCGTGGATTT; CCAGGAAGAAGCAACCATGGATACCACGATGGCCCCAAACTCTCTGCAAACATCCATGCC; AGGAAACAAAGCCCAGGAGCCCGAGATGATGCATGACGCATGGCATTTCCAAGAGCCCTG; ACAGCTCTAAGATATTAGCTACTTTCTGTATGCACAAGCTTCCCAGCTTTGTCCCCACAG; TGTACCTTTTTGCTAAAACACTTATTACCCTTCTGCAGCAAAGGCATTAAAAGCGCTAAG; CATATATTAATAAATGCAAGTGGCTAGAAATAGTGTAGGTCCCCTTCTTGCTTTCAATAT; CTTGTTGAAATAAAATGTGTCAATTGTCTCTGTGATTTAGAAACACTATTAATAACATCA; GAGCAAGGTTCTAAGGGTCTCAGCATTTGATCATCACTTTTTCTTAGCTGTCTTAAGCAT; TATAGAATTTCTCTTACCAGCATGACACTATTATATTCAGGAAACATGGCACTGCTTTTT; TCTCTAAGCAAAGGCAAATATCCTCATAATTCTAAGCTAATTCATTTAAACTTTATTAAA; TGGGGATTGGTGGAAAACTCCTGACTGGT;
• Restriction Sites: Please inquire
• ACCN: NM_016519
• Insert Size: 1900 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The ORF of this clone has been fully sequenced and found to contain one SNP compared with NM_016519.4.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_016519.4, NP_057603.1
• RefSeq Size: 1999 bp
• RefSeq ORF: 1344 bp
• Locus ID: 258
• UniProt ID: Q9NP70
• Cytogenetics: 4q13.3
• Protein Families: Secreted Protein
• Summary: This gene encodes the nonamelogenin enamel matrix protein ameloblastin. The encoded protein may be important in enamel matrix formation and mineralization. This gene is located in the calcium-binding phosphoprotein gene cluster on chromosome 4. Mutations in this gene may be associated with dentinogenesis imperfect and autosomal dominant amylogenesis imperfect. [provided by RefSeq, Aug 2011]