Acetylcholinesterase (ACHE) (NM_015831) Human Untagged Clone

SKU SC304353

ACHE (untagged)-Human acetylcholinesterase (ACHE), transcript variant E4-E5

Product Data

• Type: Human Untagged Clone
• Target Symbol: Acetylcholinesterase
• Synonyms: ACEE; ARACHE; N-ACHE; YT
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_015831 edited; GAACATCGGCCGCCTCCAGCTCCCGGCGCGGCCCGGCCCGGCCCGGCTCGGCCGCCTCAG; CAGACGCCGCCTGCCCTGCAGCCATGAGGCCCCCGCAGTGTCTGCTGCACACGCCTTCCC; TGGCTTCCCCACTCCTTCTCCTCCTCCTCTGGCTCCTGGGTGGAGGAGTGGGGGCTGAGG; GCCGGGAGGATGCAGAGCTGCTGGTGACGGTGCGTGGGGGCCGGCTGCGGGGCATTCGCC; TGAAGACCCCCGGGGGCCCTGTCTCTGCTTTCCTGGGCATCCCCTTTGCGGAGCCACCCA; TGGGACCCCGTCGCTTTCTGCCACCGGAGCCCAAGCAGCCTTGGTCAGGGGTGGTAGACG; CTACAACCTTCCAGAGTGTCTGCTACCAATATGTGGACACCCTATACCCAGGTTTTGAGG; GCACCGAGATGTGGAACCCCAACCGTGAGCTGAGCGAGGACTGCCTGTACCTCAACGTGT; GGACACCATACCCCCGGCCTACATCCCCCACCCCTGTCCTCGTCTGGATCTATGGGGGTG; GCTTCTACAGTGGGGCCTCCTCCTTGGACGTGTACGATGGCCGCTTCTTGGTACAGGCCG; AGAGGACTGTGCTGGTGTCCATGAACTACCGGGTGGGAGCCTTTGGCTTCCTGGCCCTGC; CGGGGAGCCGAGAGGCCCCGGGCAATGTGGGTCTCCTGGATCAGAGGCTGGCCCTGCAGT; GGGTGCAGGAGAACGTGGCAGCCTTCGGGGGTGACCCGACATCAGTGACGCTGTTTGGGG; AGAGCGCGGGAGCCGCCTCGGTGGGCATGCACCTGCTGTCCCCGCCCAGCCGGGGCCTGT; TCCACAGGGCCGTGCTGCAGAGCGGTGCCCCCAATGGACCCTGGGCCACGGTGGGCATGG; GAGAGGCCCGTCGCAGGGCCACGCAGCTGGCCCACCTTGTGGGCTGTCCTCCAGGCGGCA; CTGGTGGGAATGACACAGAGCTGGTAGCCTGCCTTCGGACACGACCAGCGCAGGTCCTGG; TGAACCACGAATGGCACGTGCTGCCTCAAGAAAGCGTCTTCCGGTTCTCCTTCGTGCCTG; TGGTAGATGGAGACTTCCTCAGTGACACCCCAGAGGCCCTCATCAACGCGGGAGACTTCC; ACGGCCTGCAGGTGCTGGTGGGTGTGGTGAAGGATGAGGGCTCGTATTTTCTGGTTTACG; GGGCCCCAGGCTTCAGCAAAGACAACGAGTCTCTCATCAGCCGGGCCGAGTTCCTGGCCG; GGGTGCGGGTCGGGGTTCCCCAGGTAAGTGACCTGGCAGCCGAGGCTGTGGTCCTGCATT; ACACAGACTGGCTGCATCCCGAGGACCCGGCACGCCTGAGGGAGGCCCTGAGCGATGTGG; TGGGCGACCACAATGTCGTGTGCCCCGTGGCCCAGCTGGCTGGGCGACTGGCTGCCCAGG; GTGCCCGGGTCTACGCCTACGTCTTTGAACACCGTGCTTCCACGCTCTCCTGGCCCCTGT; GGATGGGGGTGCCCCACGGCTACGAGATCGAGTTCATCTTTGGGATCCCCCTGGACCCCT; CTCGAAACTACACGGCAGAGGAGAAAATCTTCGCCCAGCGACTGATGCGATACTGGGCCA; ACTTTGCCCGCACAGGGGATCCCAATGAGCCCCGAGACCCCAAGGCCCCACAATGGCCCC; CGTACACGGCGGGGGCTCAGCAGTACGTTAGTCTGGACCTGCGGCCGCTGGAGGTGCGGC; GGGGGCTGCGCGCCCAGGCCTGCGCCTTCTGGAACCGCTTCCTCCCCAAATTGCTCAGCG; CCACCGCCTCGGAGGCTCCCAGCACCTGCCCAGGCTTCACCCATGGGGAGGCTGCTCCGA; GGCCCGGCCTCCCCCTGCCCCTCCTCCTCCTCCACCAGCTTCTCCTCCTCTTCCTCTCCC; ACCTCCGGCGGCTGTGAACACGGCCTCTTCCCCTACGGCCACAGGG;
• Restriction Sites: Please inquire
• ACCN: NM_015831
• Insert Size: 2000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The ORF of this clone has been fully sequenced and found to be a perfect match to NM_015831.1.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_015831.1, NP_056646.1
• RefSeq Size: 2909 bp
• RefSeq ORF: 1854 bp
• Locus ID: 43
• UniProt ID: P22303
• Cytogenetics: 7q22.1
• Protein Families: Druggable Genome
• Protein Pathways: Glycerophospholipid metabolism
• Summary: Acetylcholinesterase hydrolyzes the neurotransmitter, acetylcholine at neuromuscular junctions and brain cholinergic synapses, and thus terminates signal transmission. It is also found on the red blood cell membranes, where it constitutes the Yt blood group antigen. Acetylcholinesterase exists in multiple molecular forms which possess similar catalytic properties, but differ in their oligomeric assembly and mode of cell attachment to the cell surface. It is encoded by the single ACHE gene, and the structural diversity in the gene products arises from alternative mRNA splicing, and post-translational associations of catalytic and structural subunits. The major form of acetylcholinesterase found in brain, muscle and other tissues is the hydrophilic species, which forms disulfide-linked oligomers with collagenous, or lipid-containing structural subunits. The other, alternatively spliced form, expressed primarily in the erythroid tissues, differs at the C-terminal end, and contains a cleavable hydrophobic peptide with a GPI-anchor site. It associates with the membranes through the phosphoinositide (PI) moieties added post-translationally. AChE activity may constitute a sensitive biomarker of RBC ageing in vivo, and thus, may be of aid in understanding the effects of transfusion[provided by RefSeq, Sep 2019]; Transcript Variant: The splice variant E4-E5 is primarily expressed in erythroid tissues, and appears to encode the PI-linked form of acetylcholinesterase. It results from tissue-specific alternative splicing of exon 4 to exon 5.