AMMECR1 (NM_015365) Human Untagged Clone

CAT#: SC304300

AMMECR1 (untagged)-Human Alport syndrome, mental retardation, midface hypoplasia and elliptocytosis chromosomal region gene 1 (AMMECR1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: AMMECR1
• Synonyms: AMMERC1; MFHIEN
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC304300 representing NM_015365.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGCGGCGGGTTGCTGCGGGGTGAAGAAGCAGAAACTGTCCAGTTCGCCCCCCTCTGGCTCGGGTGGC
  GGTGGTGGCGCCTCCTCCTCCTCCCACTGCAGCGGAGAGAGCCAGTGCCGAGCTGGGGAGCTGGGACTA
  GGAGGCGCCGGTACGCGGCTCAACGGGCTGGGAGGTCTAACCGGAGGAGGTAGCGGCAGCGGCTGTACC
  CTCTCTCCCCCCCAGGGCTGCGGCGGCGGCGGCGGGGGGATCGCCCTGTCGCCACCTCCGAGCTGCGGA
  GTGGGGACCCTACTTTCTACCCCGGCCGCCGCCACCTCTTCCTCACCCTCCTCATCGTCCGCCGCCTCG
  TCCTCATCGCCGGGCTCCCGGAAGATGGTGGTGTCAGCAGAGATGTGCTGCTTTTGCTTCGATGTGCTC
  TACTGTCACCTGTATGGATACCAGCAGCCCCGGACCCCCCGATTCACCAACGAGCCCTACCCACTGTTT
  GTAACATGGAAGATTGGTCGAGACAAAAGATTACGTGGATGCATAGGTACTTTTTCTGCCATGAATTTG
  CATTCAGGACTCAGGGAGTACACACTTACCAGTGCCCTTAAAGATAGCCGTTTTCCCCCAATGACAAGG
  GATGAGCTGCCACGGCTTTTCTGCTCAGTGTCTCTGCTCACTAACTTTGAAGATGTCTGTGATTATTTG
  GACTGGGAGGTGGGTGTACATGGCATTAGAATAGAATTCATCAATGAAAAAGGATCAAAACGCACCGCC
  ACCTACCTACCGGAGGTTGCAAAGGAGCAAGGATGGGACCATATACAGACCATAGACTCCTTATTGAGG
  AAAGGAGGATACAAAGCTCCGATTACTAATGAATTCAGGAAAACCATAAAACTGACCAGGTATCGTAGT
  GAAAAGATGACCCTGAGCTATGCTGAATACCTTGCTCATCGCCAGCATCATCATTTCCAAAATGGCATT
  GGGCATCCCCTTCCGCCATACAACCATTATTCCTGA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_015365
• Insert Size: 1002 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015365.2
• RefSeq Size: 5431 bp
• RefSeq ORF: 1002 bp
• Locus ID: 9949
• UniProt ID: Q9Y4X0
• Cytogenetics: Xq23
• Protein Families: Druggable Genome
• MW: 35.5 kDa
• Gene Summary: The exact function of this gene is not known, however, submicroscopic deletion of the X chromosome including this gene, COL4A5, and FACL4 genes, result in a contiguous gene deletion syndrome, the AMME complex (Alport syndrome, mental retardation, midface hypoplasia, and elliptocytosis). Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2010]
  Transcript Variant: This variant (1) encodes the longest isoform (1).