NPHS2 (NM_014625) Human Untagged Clone

CAT#: SC304144

NPHS2 (untagged)-Human nephrosis 2, idiopathic, steroid-resistant (podocin) (NPHS2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NPHS2
• Synonyms: PDCN; SRN1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_014625 edited
  CCGGGATCGCGGACCCGCAGCGACTCCACAGGGACTGCGCTCCCGTGCCCCTAGCGCTCC
  CGCGCTGCTGCTCCAGCCGCCCGGCAGCTCTGAGGATGGAGAGGAGGGCGCGGAGCTCCT
  CCAGGGAGTCCCGCGGGCGAGGCGGCAGGACTCCGCACAAGGAGAACAAGAGGGCAAAGG
  CCGAGAGGAGCGGCGGGGGCCGCGGGCGCCAGGAGGCTGGGCCCGAGCCGTCGGGCTCCG
  GACGGGCGGGGACCCCGGGGGAGCCCCGAGCGCCCGCCGCCACGGTGGTGGACGTGGATG
  AGGTCCGAGGCTCCGGCGAGGAGGGCACCGAGGTGGTGGCGCTGTTGGAGAGCGAGCGGC
  CCGAGGAAGGTACCAAATCCTCTGGCTTAGGGGCCTGTGAGTGGCTTCTTGTCCTCATTT
  CCCTGCTCTTCATCATCATGACCTTCCCTTTTTCCATCTGGTTCTGCGTAAAGGTTGTAC
  AAGAGTATGAAAGAGTAATTATATTCCGACTGGGACATCTGCTTCCTGGAAGAGCCAAAG
  GCCCTGGTCTTTTCTTTTTTTTGCCCTGCCTGGATACCTACCACAAGGTTGACCTTCGTC
  TCCAAACTCTGGAGATACCTTTTCATGAGATCGTGACCAAAGACATGTTTATAATGGAGA
  TAGATGCCATTTGCTACTACCGAATGGAAAATGCCTCTCTTCTCCTAAGCAGTCTTGCTC
  ATGTATCTAAAGCTGTGCAATTCCTTGTGCAAACCACTATGAAGCGTCTCCTAGCACATC
  GATCCCTCACTGAAATTCTTCTAGAGAGGAAGAGCATCGCCCAAGATGCAAAGGTTGCCT
  TGGATTCAGTGACCTGTATTTGGGGAATCAAAGTGGAGAGAATAGAAATTAAAGATGTGA
  GGTTGCCAGCTGGGCTTCAGCACTCACTGGCTGTGGAGGCTGAAGCGCAAAGACAAGCCA
  AAGTGCGGATGATTGCTGCAGAAGCGGAAAAGGCTGCTTCTGAGTCCCTGAGGATGGCAG
  CTGAGATTCTGTCAGGCACCCCTGCTGCCGTTCAGCTTCGATACCTCCACACCCTTCAGT
  CTCTGTCCACAGAGAAGCCTTCCACTGTGGTTTTACCTTTGCCATTTGACCTGCTGAATT
  GCCTGTCTTCTCCCAGCAACAGAACTCAGGGAAGCCTCCCCTTCCCAAGTCCTTCCAAAC
  CTGTTGAGCCACTAAATCCTAAAAAGAAAGACTCTCCCATGTTATAGGAAGGATGGGGCA
  TAATGTGACTGTAAAGGGGCCTGCCATAGAAAAGTCACATGCCTGAGGGAGACACTCTGT
  CCTCATTCCCTGCCCTTCCTTTGGTTGCCATATGGAATGGCCATGGAATGCACGAAGTCA
  CAATGCACCATCCATGAGAAGACAGTGAAATGATGTAATGACAGAGAAGGCAGACAACAT
  GTTTCCGTGACTCATCTAGTCAGAGCAATTATGGGAAACAGCTTTGGTCAACATTCTACT
  TTGGAAAGAATTTTGAGTCTAGATGTGGTTAAATTTTGACTTCTGGGAACTTGGTTCAGA
  TGTCCCTTTCACTGTATGTCCTCTGACCCCTTTGGCAAGGTTGCCACAGCTCCCACAGCC
  CTTCCTACAAGCACCTATCATTGGGCTTGTCACACTCTATTGCTCTTCTGTCCCGAAGAT
  GCAGTCTTCTCTCCAATGATACTACCAAGTCTTAGTTTTCCTCAACCACACTCAATCTTT
  TTGCTCCACCCTGAATTCCTCACACCTAACCCTGATAGTTACCTAAAGTGACACTTAAAT
  GTTTCAGAGTGAATGCAAAAAAGAGAGATGTACTTGGAGTCGGATATACAATTTATCCCT
  AATTAAAGCATTTAAAAGGAAAAAAAAAAAAAAAAAAAAAAAA
  
• Restriction Sites: Please inquire
• ACCN: NM_014625
• Insert Size: 1900 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: The ORF of this clone has been fully sequenced and found to be a perfect match to NM_014625.1.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014625.1, NP_055440.1
• RefSeq Size: 1853 bp
• RefSeq ORF: 1152 bp
• Locus ID: 7827
• UniProt ID: Q9NP85
• Cytogenetics: 1q25.2
• Protein Families: Transmembrane
• Gene Summary: This gene encodes a protein that plays a role in the regulation of glomerular permeability. Mutations in this gene cause steroid-resistant nephrotic syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).