ADAMDEC1 (NM_014479) Human Untagged Clone

CAT#: SC304115

ADAMDEC1 (untagged)-Human ADAM-like, decysin 1 (ADAMDEC1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ADAMDEC1
• Synonyms: M12.219
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC304115 representing NM_014479.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGCTGCGTGGGATCTCCCAGCTACCTGCAGTGGCCACCATGTCTTGGGTCCTGCTGCCTGTACTTTGG
  CTCATTGTTCAAACTCAAGCAATAGCCATAAAGCAAACACCTGAATTAACGCTCCATGAAATAGTTTGT
  CCTAAAAAACTTCACATTTTACACAAAAGAGAGATCAAGAACAACCAGACAGAAAAGCATGGCAAAGAG
  GAAAGGTATGAACCTGAAGTTCAATATCAGATGATCTTAAATGGAGAAGAAATCATTCTCTCCCTACAA
  AAAACCAAGCACCTCCTGGGGCCAGACTACACTGAAACATTGTACTCACCCAGAGGAGAGGAAATTACC
  ACGAAACCTGAGAACATGGAACACTGTTACTATAAAGGAAACATCCTAAATGAAAAGAATTCTGTTGCC
  AGCATCAGTACTTGTGACGGGTTGAGAGGATACTTCACACATCATCACCAAAGATACCAGATAAAACCT
  CTGAAAAGCACAGACGAGAAAGAACATGCCGTCTTTACATCTAACCAGGAGGAACAAGACCCAGCTAAC
  CACACATGTGGTGTGAAGAGCACTGACGGGAAACAAGGCCCAATTCGAATCTCTAGATCACTCAAAAGC
  CCAGAGAAAGAAGACTTTCTTCGGGCACAGAAATACATTGATCTCTATTTGGTGCTGGATAATGCCTTT
  TATAAGAACTATAATGAGAATCTAACTCTGATAAGAAGCTTTGTGTTTGATGTGATGAACCTACTCAAT
  GTGATATATAACACCATAGATGTTCAAGTGGCCTTGGTAGGTATGGAAATCTGGTCTGATGGGGATAAG
  ATAAAGGTGGTGCCCAGCGCAAGCACCACGTTTGACAACTTCCTGAGATGGCACAGTTCTAACCTGGGG
  AAAAAGATCCACGACCATGCTCAGCTTCTCAGCGGGATTAGCTTCAACAATCGACGTGTGGGACTGGCA
  GCTTCAAATTCCTTGTGTTCCCCATCTTCGGTTGCTGTTATTGAGGCTAAAAAAAAGAATAATGTGGCT
  CTTGTAGGAGTGATGTCACATGAGCTGGGCCATGTCCTTGGTATGCCTGATGTTCCATTCAACACCAAG
  TGTCCCTCTGGCAGTTGTGTGATGAATCAGTATCTGAGTTCAAAATTCCCAAAGGATTTCAGTACATCT
  TGCCGTGCACATTTTGAAAGATACCTTTTATCTCAGAAACCAAAGTGCCTGCTGCAAGCACCTATTCCT
  ACAAATATAATGACAACACCAGTGTGTGGGAACCACCTTCTAGAAGTGGGAGAAGACTGTGATTGTGGC
  TCTCCTAAGGAGTGTACCAATCTCTGCTGTGAAGCCCTAACGTGTAAACTGAAGCCTGGAACTGATTGC
  GGAGGAGATGCTCCAAACCATACCACAGAGTGA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_014479
• Insert Size: 1413 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014479.3
• RefSeq Size: 2348 bp
• RefSeq ORF: 1413 bp
• Locus ID: 27299
• UniProt ID: O15204
• Cytogenetics: 8p21.2
• Protein Families: Druggable Genome, Secreted Protein
• MW: 52.8 kDa
• Gene Summary: This encoded protein is thought to be a secreted protein belonging to the disintegrin metalloproteinase family. Its expression is upregulated during dendritic cells maturation. This protein may play an important role in dendritic cell function and their interactions with germinal center T cells. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) is predicted to encode the longer protein isoform (1) using a translation initiation site in the first exon.