DKK4 (NM_014420) Human Untagged Clone

CAT#: SC304098

DKK4 (untagged)-Human dickkopf homolog 4 (Xenopus laevis) (DKK4)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DKK4
• Synonyms: DKK-4
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_014420 edited
  CTGCCAGCTTAGTGGAAGCTCTGCTCTGGGTGGAGAGCAGCCTCGCTTTGGTGACGCACA
  GTGCTGGGACCCTCCAGGAGCCCCGGGATTGAAGGATGGTGGCGGCCGTCCTGCTGGGGC
  TGAGCTGGCTCTGCTCTCCCCTGGGAGCTCTGGTCCTGGACTTCAACAACATCAGGAGCT
  CTGCTGACCTGCATGGGGCCCGGAAGGGCTCACAGTGCCTGTCTGACACGGACTGCAATA
  CCAGAAAGTTCTGCCTCCAGCCCCGCGATGAGAAGCCGTTCTGTGCTACATGTCGTGGGT
  TGCGGAGGAGGTGCCAGCGAGATGCCATGTGCTGCCCTGGGACACTCTGTGTGAACGATG
  TTTGTACTACGATGGAAGATGCAACCCCAATATTAGAAAGGCAGCTTGATGAGCAAGATG
  GCACACATGCAGAAGGAACAACTGGGCACCCAGTCCAGGAAAACCAACCCAAAAGGAAGC
  CAAGTATTAAGAAATCACAAGGCAGGAAGGGACAAGAGGGAGAAAGTTGTCTGAGAACTT
  TTGACTGTGGCCCTGGACTTTGCTGTGCTCGTCATTTTTGGACGAAAATTTGTAAGCCAG
  TCCTTTTGGAGGGACAGGTCTGCTCCAGAAGAGGGCATAAAGACACTGCTCAAGCTCCAG
  AAATCTTCCAGCGTTGCGACTGTGGCCCTGGACTACTGTGTCGAAGCCAATTGACCAGCA
  ATCGGCAGCATGCTCGATTAAGAGTATGCCAAAAAATAGAAAAGCTATAAATATTTCAAA
  ATAAAGAAGAATCCACATTGCAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_014420 unedited
  GGTCAGTTAAATTTGTATACGACTCATATAGGCGGCCGCGNATTCGCCCTTCTGCCAGCT
  TAGTGGAAGCTCTGCTCTGGGTGGAGAGCAGCCTCGCTTTGGTGACGCACAGTGCTGGGA
  CCCTCCAGGAGCCCCGGGATTGAAGGATGGTGGCGGCCGTCCTGCTGGGGCTGAGCTGGC
  TCTGCTCTCCCCTGGGAGCTCTGGTCCTGGACTTCAACAACATCAGGAGCTCTGCTGACC
  TGCATGGGGCCCGGAAGGGCTCACAGTGCCTGTCTGACACGGACTGCAATACCAGAAAGT
  TCTGCCTCCAGCCCCGCGATGAGAAGCCGTTCTGTGCTACATGTCGTGGGTTGCGGAGGA
  GGTGCCAGCGAGATGCCATGTGCTGCCCTGGGACACTCTGTGTGAACGATGTTTGTACTA
  CGATGGAAGATGCAACCCCAATATTAGAAAGGCAGCTTGATGAGCAAGATGGCACACATG
  CAGAAGGAACAACTGGGCACCCAGTCCAGGAAAACCAACCCAAAAGGAAGCCAAGTATTA
  AGAAATCACAAGGCAGGAAGGGACAAGAGGGAGAAAGTTGTCTGAGAACTTTTGACTGTG
  GCCCTGGACTTTGCTGTGCTCGTCATTTTTGGACGAAAATTTGTAAGCCAGTCCTTTTGG
  AGGGACAGGTCTGCTCCAGAAGAGGGCATAAAGACACTGCTCAAGCTCCAGAAATCTTCC
  AGCGTTGCGACTGTGGCCCTGGACTACTGTGTCGAAGCCAATTGACCAGCAATCGGCAGC
  ATGCTCGATTAAGAAGTATGCCAAAAATAGAAAAGCCTATAATATTTTCANAATANAGAA
  GAATCCACATTGCANAGGGCGAATTCAGATCTGGTACC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_014420 unedited
  CATTGGNATGGCACTTCCAGGNCCAGNAAAGCACTGGGGCAGGGTCACAGGGATGCCACC
  CGGGATCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGACAAGCTTGAT
  ATCGGTACCAGATCTGAATTCGCCCTTTGCAATGTGGATTCTTCTTTATTTTGAAATATT
  TATAGCTTTTCTATTTTTTGGCATACTCTTAATCGAGCATGCTGCCGATTGCTGGTCAAT
  TGGCTTCGACACAGTAGTCCAGGGCCACAGTCGCAACGCTGGAAGATTTCTGGAGCTTGA
  GCAGTGTCTTTATGCCCTCTTCTGGAGCAGACCTGTCCCTCCAAAAGGACTGGCTTACAA
  ATTTTCGTCCAAAAATGACGAGCACAGCAAAGTCCAGGGCCACAGTCAAAAGTTCTCAGA
  CAACTTTCTCCCTCTTGTCCCTTCCTGCCTTGTGATTTCTTAATACTTGGCTTCCTTTTG
  GGTTGGTTTTCCTGGACTGGGTGCCCAGTTGTTCCTTCTGCATGTGTGCCATCTTGCTCA
  TCAAGCTGCCTTTCTAATATTGGGGTTGCATCTTCCATCGTAGTACANACATCGTTCACA
  CAGAGTGTCCCAGGGCAGCACATGGCATCTCGCTGGCACCTCCTCCGCAACCCACGACAT
  GTAGCACAGAACGGCTTCTCATCGCGGNGCTGGAGGCAGAACTTTCTGGGTATGCAGTCC
  GTGTCAGACAGGCACTGTGAGCCCCTTCGGGCCCCNATGCAGTCAGCAGAAGCTCTGATG
  TTGNTGAAAGTCAGGACCAGAGCTCCCAGGGGAGAGCAGAGCCAGCTCAGCCCCAGCAGG
  ACGGCCGCCACATCCTTCA
  
• Restriction Sites: Please inquire
• ACCN: NM_014420
• Insert Size: 800 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014420.2, NP_055235.1
• RefSeq Size: 835 bp
• RefSeq ORF: 675 bp
• Locus ID: 27121
• UniProt ID: Q9UBT3
• Cytogenetics: 8p11.21
• Protein Families: Adult stem cells, Cancer stem cells, Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein, Stem cell relevant signaling - Wnt Signaling pathway
• Protein Pathways: Wnt signaling pathway
• Gene Summary: This gene encodes a protein that is a member of the dickkopf family. The secreted protein contains two cysteine rich regions and is involved in embryonic development through its interactions with the Wnt signaling pathway. Activity of this protein is modulated by binding to the Wnt co-receptor and the co-factor kremen 2. [provided by RefSeq, Jul 2008]