PKC delta (PRKCD) (NM_006254) Human Untagged Clone

CAT#: SC303761

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PRKCD (untagged)-Human protein kinase C, delta (PRKCD), transcript variant 1



Reconstitution Protocol

USD 992.00

3 Weeks*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol PRKCD
Synonyms ALPS3; CVID9; MAY1; nPKC-delta; PKCD
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>SC303761 representing NM_006254.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGGCGCCGTTCCTGCGCATCGCCTTCAACTCCTATGAGCTGGGCTCCCTGCAGGCCGAGGACGAGGCG
AACCAGCCCTTCTGTGCCGTGAAGATGAAGGAGGCGCTCAGCACAGAGCGTGGGAAAACACTGGTGCAG
AAGAAGCCGACCATGTATCCTGAGTGGAAGTCGACGTTCGATGCCCACATCTATGAGGGGCGCGTCATC
CAGATTGTGCTAATGCGGGCAGCAGAGGAGCCAGTGTCTGAGGTGACCGTGGGTGTGTCGGTGCTGGCC
GAGCGCTGCAAGAAGAACAATGGCAAGGCTGAGTTCTGGCTGGACCTGCAGCCTCAGGCCAAGGTGTTG
ATGTCTGTTCAGTATTTCCTGGAGGACGTGGATTGCAAACAGTCTATGCGCAGTGAGGACGAGGCCAAG
TTCCCAACGATGAACCGCCGCGGAGCCATCAAACAGGCCAAAATCCACTACATCAAGAACCATGAGTTT
ATCGCCACCTTCTTTGGGCAACCCACCTTCTGTTCTGTGTGCAAAGACTTTGTCTGGGGCCTCAACAAG
CAAGGCTACAAATGCAGGCAATGTAACGCTGCCATCCACAAGAAATGCATCGACAAGATCATCGGCAGA
TGCACTGGCACCGCGGCCAACAGCCGGGACACTATATTCCAGAAAGAACGCTTCAACATCGACATGCCG
CACCGCTTCAAGGTTCACAACTACATGAGCCCCACCTTCTGTGACCACTGCGGCAGCCTGCTCTGGGGA
CTGGTGAAGCAGGGATTAAAGTGTGAAGACTGCGGCATGAATGTGCACCATAAATGCCGGGAGAAGGTG
GCCAACCTCTGCGGCATCAACCAGAAGCTTTTGGCTGAGGCCTTGAACCAAGTCACCCAGAGAGCCTCC
CGGAGATCAGACTCAGCCTCCTCAGAGCCTGTTGGGATATATCAGGGTTTCGAGAAGAAGACCGGAGTT
GCTGGGGAGGACATGCAAGACAACAGTGGGACCTACGGCAAGATCTGGGAGGGCAGCAGCAAGTGCAAC
ATCAACAACTTCATCTTCCACAAGGTCCTGGGCAAAGGCAGCTTCGGGAAGGTGCTGCTTGGAGAGCTG
AAGGGCAGAGGAGAGTACTTTGCCATCAAGGCCCTCAAGAAGGATGTGGTCCTGATCGACGACGACGTG
GAGTGCACCATGGTTGAGAAGCGGGTGCTGACACTTGCCGCAGAGAATCCCTTTCTCACCCACCTCATC
TGCACCTTCCAGACCAAGGACCACCTGTTCTTTGTGATGGAGTTCCTCAACGGGGGGGACCTGATGTAC
CACATCCAGGACAAAGGCCGCTTTGAACTCTACCGTGCCACGTTTTATGCCGCTGAGATAATGTGTGGA
CTGCAGTTTCTACACAGCAAGGGCATCATTTACAGGGACCTCAAACTGGACAATGTGCTGCTGGACCGG
GATGGCCACATCAAGATTGCCGACTTTGGGATGTGCAAAGAGAACATATTCGGGGAGAGCCGGGCCAGC
ACCTTCTGCGGCACCCCTGACTATATCGCCCCTGAGATCCTACAGGGCCTGAAGTACACATTCTCTGTG
GACTGGTGGTCTTTCGGGGTCCTTCTGTACGAGATGCTCATTGGCCAGTCCCCCTTCCATGGTGATGAT
GAGGATGAACTCTTCGAGTCCATCCGTGTGGACACGCCACATTATCCCCGCTGGATCACCAAGGAGTCC
AAGGACATCCTGGAGAAGCTCTTTGAAAGGGAACCAACCAAGAGGCTGGGAGTGACCGGAAACATCAAA
ATCCACCCCTTCTTCAAGACCATAAACTGGACTCTGCTGGAAAAGCGGAGGTTGGAGCCACCTTTCAGG
CCCAAAGTGAAGTCACCCAGAGACTACAGTAACTTTGACCAGGAGTTCCTGAACGAGAAGGCGCGCCTC
TCCTACAGCGACAAGAACCTCATCGACTCCATGGACCAGTCTGCATTCGCTGGCTTCTCCTTTGTGAAC
CCCAAATTCGAGCACCTCCTGGAAGATTGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAG
GTTTAAACGGCCGGC
Restriction Sites SgfI-MluI      Plasmid Map     
ACCN NM_006254
Insert Size 2031 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_006254.3
RefSeq Size 2850 bp
RefSeq ORF 2031 bp
Locus ID 5580
UniProt ID Q05655
Cytogenetics 3p21.1
Domains pkinase, S_TK_X, TyrKc, DAG_PE-bind, S_TKc
Protein Families Druggable Genome, Protein Kinase
Protein Pathways Chemokine signaling pathway, Fc epsilon RI signaling pathway, Fc gamma R-mediated phagocytosis, GnRH signaling pathway, Neurotrophin signaling pathway, Tight junction, Type II diabetes mellitus, Vascular smooth muscle contraction
MW 77.5 kDa
Gene Summary The protein encoded by this gene is a member of the protein kinase C family of serine- and threonine-specific protein kinases. The encoded protein is activated by diacylglycerol and is both a tumor suppressor and a positive regulator of cell cycle progression. Also, this protein can positively or negatively regulate apoptosis. Defects in this gene are a cause of autoimmune lymphoproliferative syndrome. [provided by RefSeq, Aug 2017]
Transcript Variant: This variant (1) differs in the 5' UTR and coding sequence compared to variant 4. The resulting isoform (c) is shorter at the N-terminus compared to isoform a. Variants 1, 2, 3, 6, and 7 all encode the same isoform (c).
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.