Kv1.2 (KCNA2) (NM_004974) Human Untagged Clone

SKU SC303558

KCNA2 (untagged)-Human potassium voltage-gated channel, shaker-related subfamily, member 2 (KCNA2), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: Kv1.2
• Synonyms: DEE32; EIEE32; HBK5; HK4; HUKIV; KV1.2; MK2; NGK1; RBK2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_004974 edited; GGCACATAGAAGAGTGAGCCATCATCTGGTTTCCAGCGCCAAGACTTCTGCAGGAGAGCC; TCCGCTGGGCTTTCAAGTTGGCAACGTCACACCTCCTGAGGACAGCCAGGACTCCAGCTT; TTGCTGAGCTTTGCATCTTGCCTCCTTCCTTCAAAAGGCTCCAGGCCCATGCTCGGCTGT; CTTCAAGTCCCGCATGCCCCTGGCCAGGCACCTCACCGCCCCTCCAGCCCAGCCCAATCC; TAGAGCTCACCTCCAGGACCCAGAGTCTGCCCTTCTGCCCAGAATGACTCACCATTATTT; CTAGCTCGAGTGAGAAGACGTGATGAGGAGTTGGGCTGCCTGTTGGTGCATCTCAGATTC; CTGCAGCATCTGATCAGCTCTGCAAGGAAGAGAGCTTCACGTCTCTGGTCAGCCAAGCGA; GGCTGTCTCTCCAGCTCTCAGAGAGCTCTCGGGGCTCTCCTGCAGGAGACCAGGCCAATG; CTCCTGTGCTTCCTGGGGCCAGTAGCAGCACCCTGAGCTCCCTGCCACCAGGCAGCTGAA; AGGCATAGCGTGAGGTGCTTCTCTCAGTCCCAATTATGACAGTGGCCACCGGAGACCCAG; CAGACGAGGCTGCTGCCCTCCCTGGGCACCCACAGGACACCTATGACCCAGAGGCAGACC; ACGAGTGCTGTGAGAGGGTGGTGATCAACATCTCAGGGCTGCGGTTTGAGACCCAGCTAA; AGACCTTAGCCCAGTTTCCAGAGACCCTCTTAGGGGACCCAAAGAAACGAATGAGGTACT; TTGACCCCCTCCGGAATGAGTACTTTTTCGATCGGAACCGCCCTAGCTTTGATGCCATTT; TGTACTACTACCAGTCAGGGGGCCGATTGAGGCGACCTGTGAATGTGCCCTTAGATATAT; TCTCTGAAGAAATTCGGTTTTATGAGCTGGGAGAAGAAGCGATGGAGATGTTTCGGGAAG; ATGAAGGCTACATCAAGGAGGAAGAGCGTCCTCTGCCTGAAAATGAGTTTCAGAGACAAG; TGTGGCTTCTCTTTGAATACCCAGAGAGCTCAGGGCCTGCCAGGATTATAGCTATTGTGT; CTGTCATGGTGATTCTGATCTCAATTGTCAGCTTCTGTCTGGAAACATTGCCCATCTTCC; GGGATGAGAATGAAGACATGCATGGTAGTGGGGTGACCTTCCACACCTATTCCAACAGCA; CCATCGGGTACCAGCAGTCCACTTCCTTCACAGACCCTTTCTTCATTGTAGAGACACTCT; GCATCATCTGGTTCTCCTTTGAATTCTTGGTGAGGTTCTTTGCCTGTCCCAGCAAAGCCG; GCTTCTTCACCAACATCATGAACATCATTGACATTGTGGCCATCATCCCCTACTTCATCA; CCCTGGGGACAGAGTTGGCTGAGAAGCCAGAGGACGCTCAGCAAGGCCAGCAGGCCATGT; CACTGGCCATCCTCCGTGTCATCCGGTTGGTAAGAGTCTTTAGGATTTTCAAGTTGTCCA; GACACTCCAAAGGTCTCCAGATTCTAGGTCAGACCCTCAAAGCCAGCATGAGAGAATTGG; GCCTCCTGATATTCTTTCTCTTCATAGGGGTCATCCTTTTCTCTAGTGCTGTGTATTTTG; CAGAGGCCGATGAGCGAGAGTCCCAGTTCCCCAGCATCCCAGATGCCTTCTGGTGGGCAG; TCGTCTCCATGACAACTGTAGGCTATGGAGACATGGTTCCGACTACCATTGGGGGAAAGA; TAGTGGGTTCCCTATGTGCGATTGCAGGTGTGTTAACTATTGCCTTACCGGTCCCTGTCA; TTGTGTCCAATTTCAACTACTTCTACCACCGGGAGACAGAGGGAGAGGAACAGGCCCAAT; ACTTGCAAGTGACAAGCTGTCCAAAGATCCCATCCTCCCCTGACCTAAAGAAAAGTAGAA; GTGCCTCTACCATTAGTAAGTCTGATTACATGGAGATCCAGGAGGGTGTAAATAACAGTA; ATGAGGACTTTAGAGAGGAAAACTTGAAAACAGCCAACTGTACCTTGGCTAACACAAACT; ATGTGAATATTACCAAAATGTTAACTGATGTCTGA;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004974 unedited; GTCAAATTTGTATACGACTCATATAGGGCGGCCGCGATTCAAATCTGGTACCGGTCCGGA; ATTCCCGGGATGGCACATAGAAGAGTGAGCCATCATCTGGTTTCCAGCGCCAAGACTTCT; GCAGGAGAGCCTCCGCTGGGCTTTCAAGTTGGCAACGTCACACCTCCTGAGGACAGCCAG; GACTCCAGCTTTTGCTGAGCTTTGCATCTTGCCTCCTTCCTTCAAAAGGCTCCAGGCCCA; TGCTCGGCTGTCTTCAAGTCCCGCATGCCCCTGGCCAGGCACCTCACCGCCCCTCCAGCC; CAGCCCAATCCTAGAGCTCACCTCCAGGACCCAGAGTCTGCCCTTCTGCCCAGAATGACT; CACCATTATTTCTAGCTCGAGTGAGAAGACGTGATGAGGAGTTGGGCTGCCTGTTGGTGC; ATCTCAGATTCCTGCAGCATCTGATCAGCTCTGCAAGGAAGAGAGCTTCACGTCTCTGGT; CAGCCAAGCGAGGCTGTCTCTCCAGCTCTCAGAGAGCTCTCGGNGCTCTCCTGCAGGAGA; CCAGGCCAATGCTCCTGTGCTTCCTGGGGCCAGTAGCAGCACCCTGAGCTCCCTGCCACC; AGGCAGCTGANAGGCATAGCGTGAGGTGCTTCTCTCAGTCCCAATTATGACAGTGGCCAC; CGGAGACCCAGCAGACGAAGCTGCTGCCCTCCCTGGGCACCCACAGGACACCTATGACCC; AGAGGCAGACCACGANTGCTGTGAAAGGGTGGTGATCAACATCTCAGGGCTGNCGTTTGA; GACCCAGCTAAAGACTTTANCCCAGTTTCCAGAGACCCTCTTAGGGGACCCAAAGAAACG; ATGAAGTACTTTGAACCCCCTC;
  3' Read Nucleotide Sequence

  >OriGene 3' genomic read for NM_004974 unedited; NGCCCCTTGGGNGATGGCACTTCCCAGGNCCAGNANGAACACTGGNGGNAGGGGTCACAG; GGNATGCCACCCGGGATCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGATCAG; ACATCAGTTAACATTTTGGTAATATTCACATAGTTTGTGTTAGCCAAGGTACAGTTGGCT; GTTTTCAAGTTTTCCTCTCTAAAGTCCTCATTACTGTTATTTACACCCTCCTGGATCTCC; ATGTAATCAGACTTACTAATGGTAGAGGCACTTCTACTTTTCTTTAGGTCAGGGGAGGAT; GGGATCTTTGGACAGCTTGTCACTTGCAAGTATTGGGCCTGTTCCTCTCCCTCTGTCTCC; CGGTGGTAGAAGTAGTTGAAATTGGACACAATGACAGGGACCGGTAAGGCAATAGTTAAC; ACACCTGCAATCGCACATAGGGAACCCACTATCTTTCCCCCAATGGTAGTCGGAACCATG; TCTCCATAGCCTACAGTTGTCATGGAGACGACTGCCCACCAGAAAGCATCTGGGATGCTG; GGGAACTGGGACTCTCGCTCATCGGCCTCTGCANAATACACAGCACTAGAGAAAAGGATG; ACCCCTATGAAGAGAAAGAATATCANGAGGCCCAATTCTCTCATGCTGGCTTTGAGGGTC; TGACCTAGAATCTGGAGACCTTTGGAGTGTCTGGACAACTTGAAAATCCTAAAGACTCTT; ACCAACCGGATGACACGNAGGATGGCCAGTGACATGGCCTGCTGGCCTTGCTGAGCGTCC; TCTGGCTTCTCAGCCAACTCTGTCCCCAGGGTGATGAAGTAGGGGATGATGGNCCACATG; TCAATGATGTTCATGATGTTGGTGAAAAAACCCNNNCTTGCTGGACAGGNCAAGAACCTN;
• Restriction Sites: Please inquire
• ACCN: NM_004974
• Insert Size: 2100 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_004974.2, NP_004965.1
• RefSeq Size: 2142 bp
• RefSeq ORF: 1500 bp
• Locus ID: 3737
• UniProt ID: P16389
• Cytogenetics: 1p13.3
• Protein Families: Druggable Genome, Ion Channels: Potassium, Transmembrane
• Summary: Potassium channels represent the most complex class of voltage-gated ion channels from both functional and structural standpoints. Their diverse functions include regulating neurotransmitter release, heart rate, insulin secretion, neuronal excitability, epithelial electrolyte transport, smooth muscle contraction, and cell volume. Four sequence-related potassium channel genes - shaker, shaw, shab, and shal - have been identified in Drosophila, and each has been shown to have human homolog(s). This gene encodes a member of the potassium channel, voltage-gated, shaker-related subfamily. This member contains six membrane-spanning domains with a shaker-type repeat in the fourth segment. It belongs to the delayed rectifier class, members of which allow nerve cells to efficiently repolarize following an action potential. The coding region of this gene is intronless, and the gene is clustered with genes KCNA3 and KCNA10 on chromosome 1. [provided by RefSeq, Jul 2008]; Transcript Variant: This variant (1) encodes the longer protein (isoform a).