Steroidogenic Factor 1 (NR5A1) (NM_004959) Human Untagged Clone

SKU SC303555

NR5A1 (untagged)-Human nuclear receptor subfamily 5, group A, member 1 (NR5A1)

Product Data

• Type: Human Untagged Clone
• Target Symbol: Steroidogenic Factor 1
• Synonyms: AD4BP; ELP; FTZ1; FTZF1; hSF-1; POF7; SF-1; SF1; SPGF8; SRXX4; SRXY3
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_004959 edited; GGAGGACGGACGGACAGGGCCAGCCTGCTGTCCGGCTGCCGCCCGCCGTGGTGTGAGGGG; GTTTCTGCGCACCCACAGTCGCCACCGTCCCACCTGGGCTGCCGGAGCCTCCCCCTGGAC; CCCTGGTGCCCACTGCCACCCTCATCCGGTGTGAGAGCGCTGCTTCCGCTTCGCGGACGC; CGCGGGCATGGACTATTCGTACGACGAGGACCTGGACGAGCTGTGCCCCGTGTGCGGGGA; CAAGGTGTCCGGCTACCACTACGGACTGCTCACGTGTGAGAGCTGCAAGGGCTTCTTCAA; GCGCACGGTGCAGAACAACAAGCACTACACGTGCACCGAGAGCCAGAGCTGCAAGATCGA; CAAGACGCAGCGCAAGCGCTGTCCCTTCTGCCGCTTCCAGAAATGCCTGACGGTGGGGAT; GCGCCTGGAAGCCGTGCGCGCTGACCGTATGAGGGGTGGCCGGAACAAGTTTGGGCCGAT; GTACAAGCGGGACCGGGCCCTGAAACAGCAGAAGAAGGCACAGATTCGGGCCAATGGCTT; CAAGCTGGAGACAGGGCCCCCGATGGGGGTGCCCCCGCCGCCCCCTCCCGCACCGGACTA; CGTGCTGCCTCCCAGCCTGCATGGGCCTGAGCCCAAGGGCCTGGCCGCCGGTCCACCTGC; TGGGCCACTGGGCGACTTTGGGGCCCCAGCACTGCCCATGGCCGTGCCCGGTGCCCACGG; GCCACTGGCTGGCTACCTCTACCCTGCCTTTCCTGGCCGTGCCATCAAGTCTGAGTACCC; GGAGCCTTATGCCAGCCCCCCACAGCCTGGGCTGCCGTACGGCTACCCAGAGCCCTTCTC; TGGAGGGCCCAACGTGCCTGAGCTCATCCTGCAGCTGCTGCAGCTGGAGCCGGATGAGGA; CCAGGTGCGGGCCCGCATCTTGGGCTGCCTGCAGGAGCCCACCAAAAGCCGCCCCGACCA; GCCGGCGGCCTTCGGCCTCCTGTGCAGAATGGCCGACCAGACCTTCATCTCCATCGTGGA; CTGGGCACGCAGGTGCATGGTCTTCAAGGAGCTGGAGGTGGCCGACCAGATGACGCTGCT; GCAGAACTGCTGGAGCGAGCTGCTGGTGTTCGACCACATCTACCGCCAGGTCCAGCACGG; CAAGGAGGGCAGCATCCTGCTGGTCACCGGGCAGGAGGTGGAGCTGACCACAGTGGCCAC; CCAGGCGGGCTCGCTGCTGCACAGCCTGGTGTTGCGGGCGCAGGAGCTGGTGCTGCAGCT; GCTTGCGCTGCAGCTGGACCGGCAGGAGTTTGTCTGCCTCAAGTTCATCATCCTCTTCAG; CCTGGATTTGAAGTTCCTGAATAACCACATCCTGGTGAAAGACGCTCAGGAGAAGGCCAA; CGCCGCCCTGCTTGACTACACCCTGTGCCACTACCCGCACTGCGGGGACAAATTCCAGCA; GCTGCTGCTGTGCCTGGTGGAGGTGCGGGCCCTGAGCATGCAGGCCAAGGAGTACCTGTA; CCACAAGCACCTGGGCAACGAGATGCCCCGCAACAACCTGCTCATCGAAATGCTGCAAGC; CAAGCAGACTTGAGCCTGGGCCGGGGGCGGGGCCGGGACTGGGGGCGGGACTGGGGGCGG; GGCCTGGGCGGGGCCGCAGCCACACCGCTGGCTCTGCATGGTTCATTTTCTGATGCCCAC; CGAGGAGCCCCAGCCCCGTCCCAGAGGCCGCTGCCCCTGAGTTCTGACACTGTGTGTTTG; GGAAGTGGGTGAGGCTGGGCAGGGCCTGGCGGAGGTGGAGTGGCCACTGGCACTTGCCTG; CTGCTTGGAGTGCCCCAAGGAGGTGGCTGTTAACCACCCGCCCCGCCCCCTCCCTGCTCC; CAGCTCTCTCTCCTGGAGTCTGAAGCCTGCAGGTCCGGGGAGGAGGTTCGGGATTCCCTG; GTGGGCCTCGACGTCCCTTGGATCAGAGGTCATCCCTTCCTCCTCTCCTGGAAACAGACA; GGGAGAAGTTGAGCAGGTATCAACTAGGGGAGGAGAGAGGGTCTCCAGTGTTCCCCCCAT; AGAGACCAGGAGGGAGAGCCTCTGTTTTGTAAACTAAGGATAACCGAGTTTGCTAAATTG; AGAGGGGCTATTGGGCCCTAGAGGACACTAGGAGACTGGTTAGGACAAAAAGACCTTCTC; CCTAGCCCTTCTACCCCACCTGACCTCTGCAAGAGGGGGCATTGATACATCATCGGGAAA; AAACTTTGCTCCAGGCATCACTGATTCCCTCTCCCACCCAAGGAGAACGTTTGGTACAAT; CGACATCCTAGCCCCACCCAGAGGTGGCCCTCCCAGGCTGGTATTTATCTGCAAGGTTGT; AGTCAAGAGGTTTTTCTCCCCGCTTTTTGTTTTTAAGCTTCTAGACACTCCTTGAAATGT; GTGTGTGATGGAGGGAAGGGGACAGATTTGAGGACTGAAGCTGGGGCTTGGGGATTGCCA; CTAAGTACAGCTGATGGTTTCTCCCCGGACACTCGCCTACTAAGTACCCTTGGGGTGGTG; CTGGGTCATTACTTCTGAGCCCCAGCCCCAATCCAGAGAAGCGCTGTTGCCCGCCCTCCA; CCCACTAGGTGAACAGCAGGATGCCCTGTTGGGGGCTTCAGGTCTCTGTGGGTGGGAATG; CAAGTGAACTTGGGAGGGGGCACGGGCCTGTAGATCAGGGATAGCGCTGTTGATCCCCTC; TCTGTGGCTCCAACCCGTTGGGTCCCTTGCTGCAAACCCATGAAGCTGGCCCTCAGCTCC; CTGACCCCCTGTCCTAGGTCATGAAGGACACTCTGCAGGGTGAAGCACCAGGGAGAGGCC; TCGGCTGTCTCCTGTCCCCGGCGGGGTGCCTGCTGTCCGTCCCGCTTTCATGTTACTGTT; GCAGCTTGTGCTGAGCCTGCCCAGTTGGAGGAGACTGGGCACCCCTGCCTCCTGCCTCCC; GCCTCCCGCCACCCTGTCTCAGTACCTCCCCCCCCCGCCCCCTGAAACATGTGCCCCTGC; CAAGGCCGGAGACCCACAGCCCTGAAACGAGAAGTGCCCTTAAGGATCACCCCAGCCCCC; ACAGCCCTGGAATAAATTTCGCAATTAGTTTCCAAAAAAAAAAAAAAAAAAAAAAAAAA;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004959 unedited; NNGGTCCAGTTCAGATATTTGTATACGACTCATATAGGGCGGCCGCGAATTCCCGGATAG; GAGGACGGACGGACAGGGCCAGCCTGCTGTCCGGCTGCCGCCCGCCGTGGTGTGAGGGGG; TTTCTGCGCACCCACAGTCGCCACCGTCCCACCTGGGCTGCCGGAGCCTCCCCCTGGACC; CCTGGTGCCCACTGCCACCCTCATCCGGTGTGAGAGCGCTGCTTCCGCTTCGCGGACGCC; GCGGGCATGGACTATTCGTACGACGAGGACCTGGACGAGCTGTGCCCCGTGTGCGGGGAC; AAGGTGTCCGGCTACCACTACGGACTGCTCACGTGTGAGAGCTGCAAGGGCTTCTTCAAG; CGCACGGTGCAGAACAACAAGCACTACACGTGCACCGAGAGCCAGAGCTGCAAGATCGAC; AAGACGCAGCGCAAGCGCTGTCCCTTCTGCCGCTTCCAGAAATGCCTGACGGTGGGGATG; CGCCTGGAAGCCGTGCGCGCTGACCGTATGAGGGGTGGCCGGAACAAGTTTGGGCCGATG; TACAAGCGGGACCGGGCCCTGAAACAGCAGAAGAAGGCACAGATTCGGGCCAATGGCTTC; AAGCTGGAGACAGGGCCCCCGATGGGGGTGCCCCCGCCGCCCCCTCCCGCACCGGACTAC; GTGCTGCCTCCCAGCCTGCATGGGCCTGAGCCCAAGGGCCTGGCCGCCGGTCCACCTGCT; GGGCCACTGGGCGACTTTGGGGCCCCAGCACTGCCCATGGCCGTGCCCGGTGCCCACGGG; CCACTGGCTGGCTACCTCTACCCCTGCCTTTCTGGCCGTGCCATCAAGTCTGAGTACCCG; GAGCCTTATGCCAGCCCCCCACAGCCTGGGCTGCCGTACAGCTACCCAGACC;
• Restriction Sites: Please inquire
• ACCN: NM_004959
• Insert Size: 3100 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_004959.3, NP_004950.2
• RefSeq Size: 3119 bp
• RefSeq ORF: 1386 bp
• Locus ID: 2516
• UniProt ID: Q13285
• Cytogenetics: 9q33.3
• Protein Families: Druggable Genome, Transcription Factors
• Summary: The protein encoded by this gene is a transcriptional activator involved in sex determination. The encoded protein binds DNA as a monomer. Defects in this gene are a cause of XY sex reversal with or without adrenal failure as well as adrenocortical insufficiency without ovarian defect. [provided by RefSeq, Jul 2008]