LIPF (NM_004190) Human Untagged Clone

SKU SC303449

LIPF (untagged)-Human lipase, gastric (LIPF), transcript variant 2

Product Data

• Type: Human Untagged Clone
• Target Symbol: LIPF
• Synonyms: GL; HGL; HLAL
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_004190, RT-PCR generated; AAATGTGGCTGCTTTTAACAATGGCAAGTTTGATATCTGTACTGGGGACTACACATGGTT; TGTTTGGAAAATTACATCCTGGAAGCCCTGAAGTGACTATGAACATTAGTCAGATGATTA; CTTATTGGGGATACCCAAATGAAGAATATGAAGTTGTGACTGAAGATGGTTATATTCTTG; AAGTCAATAGAATTCCTTATGGGAAGAAAAATTCAGGGAATACAGGCCAGAGACCTGTTG; TGTTTTTGCAGCATGGTTTGCTTGCATCAGCCACAAACTGGATTTCCAACCTGCCGAACA; ACAGCCTTGCCTTCATTCTGGCAGATGCTGGTTATGATGTGTGGCTGGGCAACAGCAGAG; GAAACACCTGGGCCAGAAGAAACTTGTACTATTCACCAGATTCAGTTGAATTCTGGGCTT; TCAGCTTTGATGAAATGGCTAAATATGACCTTCCAGCCACAATCGACTTCATTGTAAAGA; AAACTGGACAGAAGCAGCTACACTATGTTGGCCATTCCCAGGGCACCACCATTGGTTTTA; TTGCCTTTTCCACCAATCCCAGCCTGGCTAAAAGAATCAAAACCTTCTATGCTCTAGCTC; CTGTTGCCACTGTGAAGTATACAAAAAGCCTTATAAACAAACTTAGATTTGTTCCTCAAT; CCCTCTTCAAGTTTATATTTGGTGACAAAATATTCTACCCACACAACTTCTTTGATCAAT; TTCTTGCTACTGAAGTGTGCTCCCGTGAGATGCTGAATCTCCTTTGCAGCAATGCCTTAT; TTATAATTTGTGGATTTGACAGTAAGAACTTTAACACGAGTCGCTTGGATGTGTATCTAT; CACATAATCCAGCAGGAACTTCTGTTCAAAACATGTTCCATTGGACCCAGGCTGTTAAGT; CTGGGAAATTCCAAGCTTATGACTGGGGAAGCCCAGTTCAGAATAGGATGCACTATGATC; AGTCCCAACCTCCCTACTACAATGTGACAGCCATGAATGTACCAATTGCAGTGTGGAACG; GTGGCAAGGACCTGTTGGCTGACCCCCAAGATGTTGGCCTTTTGCTTCCAAAACTCCCCA; ATCTTATTTACCACAAGGAGATTCCTTTTTACAATCACTTGGACTTTATCTGGGCAATGG; ATGCCCCTCAAGAAGTTTACAATGACATTGTTTCTATGATATCAGAAGATAAAAAGTAGT; TCTGGATTTAAAGAATTATCCGTTTGTTTTT;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004190 unedited; NNNNNNAAGTTCACTATTGTATACGACTCCTATAGGCGGCCGCGNATTCAGATCTGGTAC; CAAATGTGGCTGCTTTTAACAATGGCAAGTTTGATATCTGTACTGGGGACTACACATGGT; TTGTTTGGAAAATTACATCCTGGAAGCCCTGAAGTGACTATGAACATTAGTCAGATGATT; ACTTATTGGGGATACCCAAATGAAGAATATGAAGTTGTGACTGAAGATGGTTATATTCTT; GAAGTCAATAGAATTCCTTATGGGAAGAAAAATTCAGGGAATACAGGCCAGAGACCTGTT; GTGTTTTTGCAGCATGGTTTGCTTGCATCAGCCACAAACTGGATTTCCAACCTGCCGAAC; AACAGCCTTGCCTTCATTCTGGCAGATGCTGGTTATGATGTGTGGCTGGGCAACAGCAGA; GGAAACACCTGGGCCAGAAGAAACTTGTACTATTCACCAGATTCAGTTGAATTCTGGGCT; TTCAGCTTTGATGAAATGGCTAAATATGACCTTCCAGCCACAATCGACTTCATTGTAAAG; AAAACTGGACAGAAGCAGCTACACTATGTTGGCCATTCCCAGGGCACCACCATTGGGTTT; TATTGCCTTTTCCACCAATCCCAGCCTGGCTAAAAGAATCAAAACCTTCTATGCTCTAGC; TCCTGTTGCCACTGTGAAGTATACAAAAAGCCTTATAAACAAACTTAGNATTTGTTCCTC; AATCCCTCTTCAAGTTTATTATTTGGTGACAAAATATTCTACCCACACAACTTCTTTGAT; CAATTTCTTGCTACTGGAAGTGTGCTCCCGTGAGATGCTGAATCTCCTTTGCAGCAATGC; CTTATTTATAATTTGGTGGATTTGACAGTAAGAACTTTAACACGAGTCGTTTGGGGATGT; GTAT;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004190 unedited; NNCCATTGGNGAATGGCACTTNCCAGGNCCAGNANAGCACTGGGGNAGGGTCACAGGNAT; GCCACCCGGGATCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTT; GNGAAAAACAAACGGATAATTCTTTAAATCCAGAACTACTTTTTATCTTCTGATATCATA; GAAACAATGTCATTGTAAACTTCTTGAGGGGCATCCATTGCCCAGATAAAGTCCAAGTGA; TTGTAAAAAGGAATCTCCTTGTGGTAAATAAGATTGGGGAGTTTTGGAAGCAAAAGGCCA; ACATCTTGGGGGTCAGCCAACAGGTCCTTGCCACCGTTCCACACTGCAATTGGTACATTC; ATGGCTGTCACATTGTAGTAGGGAGGTTGGGACTGATCATAGTGCATCCTATTCTGAACT; GGGCTTCCCCAGTCATAAGCTTGGAATTTCCCAGACTTAACAGCCTGNGTCCAATGGAAC; ATGTTTTGAACAGAAGTTCCTGCTGGATTATGTGATAGATACACATCCAAGCGACTCGTG; TTAAAGTTCTTACTGTCAAATCCACAAATTATAAATAAGGCATTGCTGCANAGGAGATTC; AGCATCTCACGGGAGCACACTTCAGTAGCAAGAAATTGATCAAAGAAAAGTTGTGTGGGT; AGAAATATTTTGTCACCAAATATAAACTTGAAGAGGGGATTGAGGAACANATCTAAAGGT; TTGTTAATAAGGCTTTTTGTATACTTCACAGTGGCAACAGGAGCTAGACCTAANAAAGGT; TTTGATTCTTTTTACCAGGCTGGGATTGGGGGGAAAAGGCAATAAACCAATGGTTGGTGC; CCTGGGAATGGNCCACATAGTGTAACTGGCTCTGTCCCGGTTTCTTACAATGAAATCGAT; GTGGCTGGGG;
• Restriction Sites: Please inquire
• ACCN: NM_004190
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This construct is a cloned PCR fragment that was fully sequenced. There are no nucleotide differences between the OriGene clone and the NCBI reference ORF.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_004190.1, NP_004181.1
• RefSeq Size: 1365 bp
• RefSeq ORF: 1197 bp
• Locus ID: 8513
• UniProt ID: P07098
• Cytogenetics: 10q23.31
• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: Glycerolipid metabolism, Metabolic pathways
• Summary: This gene encodes gastric lipase, an enzyme involved in the digestion of dietary triglycerides in the gastrointestinal tract, and responsible for 30% of fat digestion processes occurring in human. It is secreted by gastric chief cells in the fundic mucosa of the stomach, and it hydrolyzes the ester bonds of triglycerides under acidic pH conditions. The gene is a member of a conserved gene family of lipases that play distinct roles in neutral lipid metabolism. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2010]; Transcript Variant: This variant (2) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (2) is shorter at the N-terminus compared to isoform 1.