ZIC3 (NM_003413) Human Untagged Clone

CAT#: SC303302

ZIC3 (untagged)-Human Zic family member 3 (ZIC3)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ZIC3
• Synonyms: HTX; HTX1; VACTERLX; ZNF203
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC303302 representing NM_003413.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGACGATGCTCCTGGACGGAGGCCCGCAGTTCCCTGGGCTGGGAGTGGGCAGCTTCGGCGCGCCGCGC
  CACCACGAGATGCCCAACCGTGAGCCGGCAGGCATGGGGCTGAATCCCTTCGGGGACTCAACCCACGCC
  GCCGCCGCCGCCGCCGCCGCCGCTGCCTTCAAGCTGAGCCCTGCCGCGGCGCACGATCTATCTTCAGGC
  CAGAGCTCGGCTTTCACGCCGCAGGGTTCGGGCTACGCCAACGCCCTGGGCCACCATCACCACCACCAT
  CACCATCATCACCACACCAGCCAGGTGCCCAGCTACGGTGGCGCTGCCTCTGCCGCCTTCAACTCAACG
  CGCGAGTTTCTGTTCCGCCAGCGCAGCTCCGGGCTCAGTGAGGCGGCCTCGGGTGGCGGGCAGCACGGG
  CTCTTCGCCGGCTCGGCGAGCAGCCTGCATGCTCCAGCTGGCATCCCCGAGCCCCCTAGCTACTTGCTG
  TTTCCCGGGCTGCATGAGCAGGGCGCTGGGCACCCGTCGCCCACAGGGCACGTGGACAACAACCAGGTC
  CACCTGGGGCTGCGTGGGGAGCTGTTCGGCCGTGCTGACCCATACCGCCCAGTGGCCAGCCCGCGCACG
  GACCCCTACGCGGCCGGCGCTCAGTTTCCTAACTACAGCCCCATGAACATGAACATGGGAGTGAACGTG
  GCGGCCCACCACGGGCCCGGCGCCTTCTTCCGTTATATGCGGCAGCCTATCAAGCAGGAGCTGTCGTGC
  AAGTGGATCGACGAGGCTCAGCTGAGCCGGCCCAAGAAGAGCTGCGACCGGACCTTCAGCACCATGCAT
  GAGCTGGTGACACATGTCACCATGGAGCATGTGGGGGGCCCGGAGCAGAACAACCACGTCTGCTACTGG
  GAGGAGTGCCCCCGGGAGGGCAAGTCTTTCAAGGCGAAGTACAAACTGGTCAACCACATCCGAGTGCAC
  ACGGGCGAGAAGCCCTTCCCATGCCCCTTCCCGGGCTGCGGGAAGATCTTTGCCCGTTCTGAGAACCTC
  AAGATCCACAAGAGGACCCACACAGGTGAGAAACCTTTCAAATGTGAATTTGAAGGCTGTGACAGACGC
  TTTGCCAACAGCAGCGACCGTAAGAAGCACATGCATGTGCATACCTCGGACAAGCCCTATATCTGCAAA
  GTGTGCGACAAGTCCTACACGCACCCGAGCTCCCTGCGCAAACACATGAAGGTTCATGAATCTCAAGGG
  TCAGATTCCTCCCCTGCTGCCAGTTCAGGCTATGAATCTTCCACTCCACCCGCTATAGCTTCTGCAAAC
  AGTAAAGATACCACTAAAACCCCTTCTGCAGTTCAAACTAGCACCAGCCACAACCCTGGACTTCCTCCT
  AATTTTAACGAATGGTACGTCTGA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_003413
• Insert Size: 1404 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003413.3
• RefSeq Size: 3939 bp
• RefSeq ORF: 1404 bp
• Locus ID: 7547
• UniProt ID: O60481
• Cytogenetics: Xq26.3
• Protein Families: Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS
• MW: 50.6 kDa
• Gene Summary: This gene encodes a member of the ZIC family of C2H2-type zinc finger proteins. This nuclear protein probably functions as a transcription factor in early stages of left-right body axis formation. Mutations in this gene cause X-linked visceral heterotaxy, which includes congenital heart disease and left-right axis defects in organs. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1, also known as ZIC3-A) represents the longer transcript and encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.