APOBEC1 (NM_001644) Human Untagged Clone

CAT#: SC303051

APOBEC1 (untagged)-Human apolipoprotein B mRNA editing enzyme, catalytic polypeptide 1 (APOBEC1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: APOBEC1
• Synonyms: APOBEC-1; BEDP; CDAR1; HEPR
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001644 edited
  AGAGCACCATGACTTCTGAGAAAGGTCCTTCAACCGGTGACCCCACTCTGAGGAGAAGAA
  TCGAACCCTGGGAGTTTGACGTCTTCTATGACCCCAGAGAACTTCGTAAAGAGGCCTGTC
  TGCTCTACGAAATCAAGTGGGGCATGAGCCGGAAGATCTGGCGAAGCTCAGGCAAAAACA
  CCACCAATCACGTGGAAGTTAATTTTATAAAAAAATTTACGTCAGAAAGAGATTTTCACC
  CATCCATCAGCTGCTCCATCACCTGGTTCTTGTCCTGGAGTCCCTGCTGGGAATGCTCCC
  AGGCTATTAGAGAGTTTCTGAGTCGGCACCCTGGTGTGACTCTAGTGATCTACGTAGCTC
  GGCTTTTTTGGCACATGGATCAACAAAATCGGCAAGGTCTCAGGGACCTTGTTAACAGTG
  GAGTAACTATTCAGATTATGAGAGCATCAGAGTATTATCACTGCTGGAGGAATTTTGTCA
  ACTACCCACCTGGGGATGAAGCTCACTGGCCACAATACCCACCTCTGTGGATGATGTTGT
  ACGCACTGGAGCTGCACTGCATAATTCTAAGTCTTCCACCCTGTTTAAAGATTTCAAGAA
  GATGGCAAAATCATCTTACATTTTTCAGACTTCATCTTCAAAACTGCCATTACCAAACGA
  TTCCGCCACACATCCTTTTAGCTACAGGGCTGATACATCCTTCTGTGGCTTGGAGATGAA
  TAGGATGATTCCGTGTGTGTACTGATTCAAGAACAAGCAATGATGACCCAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001644 unedited
  GTTCAGTTCAGATTTGTATACGACTCACTATAGGCGGCCGCGACTTCGCCCTTAGAGCAC
  CATGACTTCTGAGAAAGTCCTTCAACCGGTGACCCCACTCTGAGGAGAAGAATCGAACCC
  TGGGAGTTTGACGTCTTCTATGACCCCAGAGAACTTCGTAAAGAGGCCTGTCTGCTCTAC
  GAAATCAAGTGGGGCATGAGCCGGAAGATCTGGCGAAGCTCAGGCAAAAACACCACCAAT
  CACGTGGAAGTTAATTTTATAAAAAAATTTACGTCAGAAAGAGATTTTCACCCATCCATC
  AGCTGCTCCATCACCTGGTTCTTGTCCTGGAGTCCCTGCTGGGAATGCTCCCAGGCTATT
  AGAGAGTTTCTGAGTCGGCACCCTGGTGTGACTCTAGTGATCTACGTAGCTCGGCTTTTT
  TGGCACATGGATCAACAAAATCGGCAAGGTCTCAGGGACCTTGTTAACAGTGGAGTAACT
  ATTCAGATTATGAGAGCATCAGAGTATTATCACTGCTGGAGGAATTTTGTCAACTACCCA
  CCTGGGGATGAAGCTCACTGGCCACAATACCCACCTCTGTGGATGATGTTGTACGCACTG
  GAGCTGCACTGCATAATTCTAAGTCTTCCACCCTGTTTAAAGATTTCAAGAAGATGGCAA
  AATCATCTTACATTTTTCAGACTTCATCTTCAAAACTGCCATTACCAAACGATTCCGCCA
  CACATCCTTTTAGCTACAGGGCTGATACATCCTTCTGTGGCTTGGAGATGAATAGGATGA
  TTCCGTGTGTGTACTGGATTCAGAACAAGCATGATGACCCACAGGGCGAATCAGATCTGT
  ACCGATATCAGCTTGTCGACTCTAGATTGCGCCGCGTCATAGCTGNTTCCTGAACGATCC
  CGGTGN
  
• Restriction Sites: Please inquire
• ACCN: NM_001644
• Insert Size: 800 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: There is 1 nucleotide difference between the OriGene clone and the NCBI reference ORF. These result in the substitution of 1 aa.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001644.3, NP_001635.2
• RefSeq Size: 911 bp
• RefSeq ORF: 711 bp
• Locus ID: 339
• UniProt ID: P41238
• Cytogenetics: 12p13.31
• Gene Summary: This gene encodes a member of the cytidine deaminase enzyme family. The encoded protein forms a multiple-protein editing holoenzyme with APOBEC1 complementation factor (ACF) and APOBEC1 stimulating protein (ASP). This holoenzyme is involved in the editing of C-to-U nucleotide bases in apolipoprotein B and neurofibromatosis-1 mRNAs. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2015]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (a). Variants 1 and 2 both encode the same isoform (a).