Alkaline Phosphatase (ALPI) (NM_001631) Human Untagged Clone

CAT#: SC303050

ALPI (untagged)-Human alkaline phosphatase, intestinal (ALPI)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Alkaline Phosphatase
• Synonyms: IAP
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001631 edited
  ACTTCGCCTCCCTCCTGCTGCCCCCAAGACATGCAGGGGCCCTGGGTGCTGCTGCTGCTG
  GGCCTGAGGCTACAGCTCTCCCTGGGCGTCATCCCAGCTGAGGAGGAGAACCCGGCCTTC
  TGGAACCGCCAGGCAGCTGAGGCCCTGGATGCTGCCAAGAAGCTGCAGCCCATCCAGAAG
  GTCGCCAAGAACCTCATCCTCTTCCTGGGCGATGGGTTGGGGGTGCCCACGGTGACAGCC
  ACCAGGATCCTAAAGGGGCAGAAGAATGGCAAACTGGGGCCTGAGACGCCCCTGGCCATG
  GACCGCTTCCCATACCTGGCTCTGTCCAAGACATACAATGTGGACAGACAGGTGCCAGAC
  AGCGCAGCCACAGCCACGGCCTACCTGTGCGGGGTCAAGGCCAACTTCCAGACCATCGGC
  TTGAGTGCAGCCGCCCGCTTTAACCAGTGCAACACGACACGCGGCAATGAGGTCATCTCC
  GTGATGAACCGGGCCAAGCAAGCAGGAAAGTCAGTAGGAGTGGTGACCACCACACGGGTG
  CAGCACGCCTCGCCAGCCGGCACCTACGCACACACAGTGAACCGCAACTGGTACTCAGAT
  GCTGACATGCCTGCCTCAGCCCGCCAGGAGGGGTGCCAGGACATCGCCACTCAGCTCATC
  TCCAACATGGACATTGACGTGATCCTTGGCGGAGGCCGCAAGTACATGTTTCCCATGGGG
  ACCCCAGACCCTGAGTACCCAGCTGATGCCAGCCAGAATGGAATCAGGCTGGACGGGAAG
  AACCTGGTGCAGGAATGGCTGGCAAAGCACCAGGGTGCCTGGTATGTGTGGAACCGCACT
  GAGCTCATGCAGGCGTCCCTGGACCAGTCTGTGACCCATCTCATGGGCCTCTTTGAGCCC
  GGAGACACGAAATATGAGATCCACCGAGACCCCACACTGGACCCCTCCCTGATGGAGATG
  ACAGAGGCTGCCCTGCGCCTGCTGAGCAGGAACCCCCGCGGCTTCTACCTCTTTGTGGAG
  GGCGGCCGCATCGACCATGGTCATCATGAGGGTGTGGCTTACCAGGCACTCACTGAGGCG
  GTCATGTTCGACGACGCCATTGAGAGGGCGGGCCAGCTCACCAGCGAGGAGGACACGCTG
  ACCCTCGTCACCGCTGACCACTCCCATGTCTTCTCCTTTGGTGGCTACACCTTGCGAGGG
  AGCTCCATCTTCGGGTTGGCCCCCAGCAAGGCTCAGGACAGCAAAGCCTACACGTCCATC
  CTGTACGGCAATGGCCCGGGCTACGTGTTCAACTCAGGCGTGCGACCAGACGTGAATGAG
  AGCGAGAGCGGGAGCCCCGATTACCAGCAGCAGGCGGCGGTGCCCCTGTCGTCCGAGACC
  CACGGAGGCGAAGACGTGGCGGTGTTTGCGCGCGGCCCGCAGGCGCACCTGGTGCATGGT
  GTGCAGGAGCAGAGCTTCGTAGCGCATGTCATGGCCTTCGCTGCCTGTCTGGAGCCCTAC
  ACGGCCTGCGACCTGGCGCCTCCCGCCTGCACCACCGACGCCGCGCACCCAGTTGCCGCG
  TCGCTGCCACTGCTGGCCGGGACCCTGCTGCTGCTGGGGGCGTCCGCTGCTCCCTGAGTG
  CCCCACTCCGGAGTTATCCTGCTCCCCACCTCCGGGCGTCCTGCCCTGTTCCCCGTCCTG
  AGCCGCCACTTCCAGCGAACACACACAGGTGTCCTGCCGTTGGACCTTCACCTCCTAGAG
  ATAAACCAGCCTCAGCTGGCGCAGCGGGGCCCTTCTTCCCTCCGCATCCCCTTCAGGGAG
  CAGGAGCCCAGGGCGCCCTGGGAGCTGAGCCTGGGACTTCCAGGACCTCCCCTCAGGTTG
  TTCTCTGATTCTTCCTCCCAACCCCAGAGACTGCAGATTTGTGCCATGCGGCTGCCTGCA
  CCCCAGACAATAAAGGGACCAAAACCACCCAACCCCCACCCTGCCTCTATCCTAAGGAAG
  ACCAAGCAGGCCTGGACCCAGAGACGTCCCCCATCGTGGGACACGACACACCCAGACCGC
  GTGCCCCACCGTCTTAGCTTCAATCCTGGCAGCACCTGGTAGACCCAAGGACTTGGGTGG
  ATCAGGACACCTGAAGAAGAGAAGCTTCCGGCAACCCTGCAACCCACCCAAGGAGGCTAC
  TGGATCGGGGATTCCCAGGGGGGCTTTGACACAGTCCTCTGCTGTCTCCCCACTAGGATC
  ATTCCACACCCCTGCACCTGACCAAGGGACCAATGAGGC
  
• Restriction Sites: Please inquire
• ACCN: NM_001631
• Insert Size: 1587 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to differ from the protein associated to this reference by three amino acids.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001631.2, NP_001622.1
• RefSeq Size: 2516 bp
• RefSeq ORF: 1587 bp
• Locus ID: 248
• UniProt ID: P09923
• Cytogenetics: 2q37.1
• Protein Families: Druggable Genome
• Protein Pathways: Folate biosynthesis, Metabolic pathways
• Gene Summary: There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The intestinal alkaline phosphatase gene encodes a digestive brush-border enzyme. This enzyme is a component of the gut mucosal defense system and is thought to function in the detoxification of lipopolysaccharide, and in the prevention of bacterial translocation in the gut. [provided by RefSeq, Dec 2014]