Alkaline Phosphatase (ALPI) (NM_001631) Human Untagged Clone

SKU SC303050

ALPI (untagged)-Human alkaline phosphatase, intestinal (ALPI)

Product Data

• Type: Human Untagged Clone
• Target Symbol: Alkaline Phosphatase
• Synonyms: IAP
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001631 edited; ACTTCGCCTCCCTCCTGCTGCCCCCAAGACATGCAGGGGCCCTGGGTGCTGCTGCTGCTG; GGCCTGAGGCTACAGCTCTCCCTGGGCGTCATCCCAGCTGAGGAGGAGAACCCGGCCTTC; TGGAACCGCCAGGCAGCTGAGGCCCTGGATGCTGCCAAGAAGCTGCAGCCCATCCAGAAG; GTCGCCAAGAACCTCATCCTCTTCCTGGGCGATGGGTTGGGGGTGCCCACGGTGACAGCC; ACCAGGATCCTAAAGGGGCAGAAGAATGGCAAACTGGGGCCTGAGACGCCCCTGGCCATG; GACCGCTTCCCATACCTGGCTCTGTCCAAGACATACAATGTGGACAGACAGGTGCCAGAC; AGCGCAGCCACAGCCACGGCCTACCTGTGCGGGGTCAAGGCCAACTTCCAGACCATCGGC; TTGAGTGCAGCCGCCCGCTTTAACCAGTGCAACACGACACGCGGCAATGAGGTCATCTCC; GTGATGAACCGGGCCAAGCAAGCAGGAAAGTCAGTAGGAGTGGTGACCACCACACGGGTG; CAGCACGCCTCGCCAGCCGGCACCTACGCACACACAGTGAACCGCAACTGGTACTCAGAT; GCTGACATGCCTGCCTCAGCCCGCCAGGAGGGGTGCCAGGACATCGCCACTCAGCTCATC; TCCAACATGGACATTGACGTGATCCTTGGCGGAGGCCGCAAGTACATGTTTCCCATGGGG; ACCCCAGACCCTGAGTACCCAGCTGATGCCAGCCAGAATGGAATCAGGCTGGACGGGAAG; AACCTGGTGCAGGAATGGCTGGCAAAGCACCAGGGTGCCTGGTATGTGTGGAACCGCACT; GAGCTCATGCAGGCGTCCCTGGACCAGTCTGTGACCCATCTCATGGGCCTCTTTGAGCCC; GGAGACACGAAATATGAGATCCACCGAGACCCCACACTGGACCCCTCCCTGATGGAGATG; ACAGAGGCTGCCCTGCGCCTGCTGAGCAGGAACCCCCGCGGCTTCTACCTCTTTGTGGAG; GGCGGCCGCATCGACCATGGTCATCATGAGGGTGTGGCTTACCAGGCACTCACTGAGGCG; GTCATGTTCGACGACGCCATTGAGAGGGCGGGCCAGCTCACCAGCGAGGAGGACACGCTG; ACCCTCGTCACCGCTGACCACTCCCATGTCTTCTCCTTTGGTGGCTACACCTTGCGAGGG; AGCTCCATCTTCGGGTTGGCCCCCAGCAAGGCTCAGGACAGCAAAGCCTACACGTCCATC; CTGTACGGCAATGGCCCGGGCTACGTGTTCAACTCAGGCGTGCGACCAGACGTGAATGAG; AGCGAGAGCGGGAGCCCCGATTACCAGCAGCAGGCGGCGGTGCCCCTGTCGTCCGAGACC; CACGGAGGCGAAGACGTGGCGGTGTTTGCGCGCGGCCCGCAGGCGCACCTGGTGCATGGT; GTGCAGGAGCAGAGCTTCGTAGCGCATGTCATGGCCTTCGCTGCCTGTCTGGAGCCCTAC; ACGGCCTGCGACCTGGCGCCTCCCGCCTGCACCACCGACGCCGCGCACCCAGTTGCCGCG; TCGCTGCCACTGCTGGCCGGGACCCTGCTGCTGCTGGGGGCGTCCGCTGCTCCCTGAGTG; CCCCACTCCGGAGTTATCCTGCTCCCCACCTCCGGGCGTCCTGCCCTGTTCCCCGTCCTG; AGCCGCCACTTCCAGCGAACACACACAGGTGTCCTGCCGTTGGACCTTCACCTCCTAGAG; ATAAACCAGCCTCAGCTGGCGCAGCGGGGCCCTTCTTCCCTCCGCATCCCCTTCAGGGAG; CAGGAGCCCAGGGCGCCCTGGGAGCTGAGCCTGGGACTTCCAGGACCTCCCCTCAGGTTG; TTCTCTGATTCTTCCTCCCAACCCCAGAGACTGCAGATTTGTGCCATGCGGCTGCCTGCA; CCCCAGACAATAAAGGGACCAAAACCACCCAACCCCCACCCTGCCTCTATCCTAAGGAAG; ACCAAGCAGGCCTGGACCCAGAGACGTCCCCCATCGTGGGACACGACACACCCAGACCGC; GTGCCCCACCGTCTTAGCTTCAATCCTGGCAGCACCTGGTAGACCCAAGGACTTGGGTGG; ATCAGGACACCTGAAGAAGAGAAGCTTCCGGCAACCCTGCAACCCACCCAAGGAGGCTAC; TGGATCGGGGATTCCCAGGGGGGCTTTGACACAGTCCTCTGCTGTCTCCCCACTAGGATC; ATTCCACACCCCTGCACCTGACCAAGGGACCAATGAGGC;
• Restriction Sites: Please inquire
• ACCN: NM_001631
• Insert Size: 1587 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to differ from the protein associated to this reference by three amino acids.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001631.2, NP_001622.1
• RefSeq Size: 2516 bp
• RefSeq ORF: 1587 bp
• Locus ID: 248
• UniProt ID: P09923
• Cytogenetics: 2q37.1
• Protein Families: Druggable Genome
• Protein Pathways: Folate biosynthesis, Metabolic pathways
• Summary: There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The intestinal alkaline phosphatase gene encodes a digestive brush-border enzyme. This enzyme is a component of the gut mucosal defense system and is thought to function in the detoxification of lipopolysaccharide, and in the prevention of bacterial translocation in the gut. [provided by RefSeq, Dec 2014]