SPINT1 (NM_001032367) Human Untagged Clone

SKU SC302639

SPINT1 (untagged)-Human serine peptidase inhibitor, Kunitz type 1 (SPINT1), transcript variant 3

Product Data

• Type: Human Untagged Clone
• Target Symbol: SPINT1
• Synonyms: HAI; HAI1; MANSC2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001032367, the custom clone sequence may differ by one or more nucleotides; ; ; ATGGCCCCTGCGAGGACGATGGCCCGCGCCCGCCTCGCCCCGGCCGGCATCCCTGCCGTCGCCTTGTGGC; TTCTGTGCACGCTCGGCCTCCAGGGCACCCAGGCCGGGCCACCGCCCGCGCCCCCTGGGCTGCCCGCGGG; AGCCGACTGCCTGAACAGCTTTACCGCCGGGGTGCCTGGCTTCGTGCTGGACACCAACGCCTCGGTCAGC; AACGGAGCTACCTTCCTGGAGTCCCCCACCGTGCGCCGGGGCTGGGACTGCGTGCGCGCCTGCTGCACCA; CCCAGAACTGCAACTTGGCGCTAGTGGAGCTGCAGCCCGACCGCGGGGAGGACGCCATCGCCGCCTGCTT; CCTCATCAACTGCCTCTACGAGCAGAACTTCGTGTGCAAGTTCGCGCCCAGGGAGGGCTTCATCAACTAC; CTCACGAGGGAAGTGTACCGCTCCTACCGCCAGCTGCGGACCCAGGGCTTTGGAGGGTCTGGGATCCCCA; AGGCCTGGGCAGGCATAGACTTGAAGGTACAACCCCAGGAACCCCTGGTGCTGAAGGATGTGGAAAACAC; AGATTGGCGCCTACTGCGGGGTGACACGGATGTCAGGGTAGAGAGGAAAGACCCAAACCAGGTGGAACTG; TGGGGACTCAAGGAAGGCACCTACCTGTTCCAGCTGACAGTGACTAGCTCAGACCACCCAGAGGACACGG; CCAACGTCACAGTCACTGTGCTGTCCACCAAGCAGACAGAAGACTACTGCCTCGCATCCAACAAGGTGGG; TCGCTGCCGGGGCTCTTTCCCACGCTGGTACTATGACCCCACGGAGCAGATCTGCAAGAGTTTCGTTTAT; GGAGGCTGCTTGGGCAACAAGAACAACTACCTTCGGGAAGAAGAGTGCATTCTAGCCTGTCGGGGTGTGC; AAGGCCCCTCCATGGAAAGGCGCCATCCAGTGTGCTCTGGCACCTGTCAGCCCACCCAGTTCCGCTGCAG; CAATGGCTGCTGCATCGACAGTTTCCTGGAGTGTGACGACACCCCCAACTGCCCCGACGCCTCCGACGAG; GCTGCCTGTGAAAAATACACGAGTGGCTTTGACGAGCTCCAGCGCATCCATTTCCCCAGTGACAAAGGGC; ACTGCGTGGACCTGCCAGACACAGGACTCTGCAAGGAGAGCATCCCGCGCTGGTACTACAACCCCTTCAG; CGAACACTGCGCCCGCTTTACCTATGGTGGTTGTTACGGCAACAAGAACAACTTTGAGGAAGAGCAGCAG; TGCCTCGAGTCTTGTCGCGGCATCTCCAAGAAGGATGTGTTTGGCCTGAGGCGGGAAATCCCCATTCCCA; GCACAGGCTCTGTGGAGATGGCTGTCGCAGTGTTCCTGGTCATCTGCATTGTGGTGGTGGTAGCCATCTT; GGGTTACTGCTTCTTCAAGAACCAGAGAAAGGACTTCCACGGACACCACCACCACCCACCACCCACCCCT; GCCAGCTCCACTGTCTCCACTACCGAGGACACGGAGCACCTGGTCTATAACCACACCACGCGGCCCCTCT; GA; ;
• Restriction Sites: NotI-NotI
• ACCN: NM_001032367
• Insert Size: 2300 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The open reading frame of this clone has been fully sequenced and found two SNPs within the protein associated with this reference, NM_001032367.1. These two SNPs don't change amino acid.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001032367.1, NP_001027539.1
• RefSeq Size: 2342 bp
• RefSeq ORF: 1542 bp
• Locus ID: 6692
• UniProt ID: O43278
• Cytogenetics: 15q15.1
• Protein Families: Druggable Genome, Secreted Protein, Transmembrane
• Summary: The protein encoded by this gene is a member of the Kunitz family of serine protease inhibitors. The protein is a potent inhibitor specific for HGF activator and is thought to be involved in the regulation of the proteolytic activation of HGF in injured tissues. Alternative splicing results in multiple variants encoding different isoforms. [provided by RefSeq, Jul 2008]; Transcript Variant: This variant (3) has multiple differences in the 5' UTR and uses an alternate in-frame splice site in the 3' coding region, compared to variant 1, resulting in a shorter protein (isoform 2), compared to isoform 1.