CRELD1 (NM_001031717) Human Untagged Clone

SKU SC302562

CRELD1 (untagged)-Human cysteine-rich with EGF-like domains 1 (CRELD1), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: CRELD1
• Synonyms: AVSD2; CIRRIN
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_001031717 edited; ATGGCCCCATGGCCCCCGAAGGGCCTAGTCCCAGCTGTGCTCTGGGGCCTCAGCCTCTTC; CTCAACCTCCCAGGACCTATCTGGCTCCAGCCCTCTCCACCTCCCCAGTCTTCTCCCCCG; CCTCAGCCCCATCCGTGTCATACCTGCCGGGGACTGGTTGACAGCTTTAACAAGGGCCTG; GAGAGAACCATCCGGGACAACTTTGGAGGTGGAAACACTGCCTGGGAGGAAGAGAATTTG; TCCAAATACAAAGACAGTGAGACCCGCCTGGTAGAGGTGCTGGAGGGTGTGTGCAGCAAG; TCAGACTTCGAGTGCCACCGCCTGCTGGAGCTGAGTGAGGAGCTGGTGGAGAGCTGGTGG; TTTCACAAGCAGCAGGAGGCCCCGGACCTCTTCCAGTGGCTGTGCTCAGATTCCCTGAAG; CTCTGCTGCCCCGCAGGCACCTTCGGGCCCTCCTGCCTTCCCTGTCCTGGGGGAACAGAG; AGGCCCTGCGGTGGCTACGGGCAGTGTGAAGGAGAAGGGACACGAGGGGGCAGCGGGCAC; TGTGACTGCCAAGCCGGCTACGGGGGTGAGGCCTGTGGCCAGTGTGGCCTTGGCTACTTT; GAGGCAGAACGCAACGCCAGCCATCTGGTATGTTCGGCTTGTTTTGGCCCCTGTGCCCGA; TGCTCAGGACCTGAGGAATCAAACTGTTTGCAATGCAAGAAGGGCTGGGCCCTGCATCAC; CTCAAGTGTGTAGACATTGATGAGTGTGGCACAGAGGGAGCCAACTGTGGAGCTGACCAA; TTCTGCGTGAACACTGAGGGCTCCTATGAGTGCCGAGACTGTGCCAAGGCCTGCCTAGGC; TGCATGGGGGCAGGGCCAGGTCGCTGTAAGAAGTGTAGCCCTGGCTATCAGCAGGTGGGC; TCCAAGTGTCTCGATGTGGATGAGTGTGAGACAGAGGTGTGTCCGGGAGAGAACAAGCAG; TGTGAAAACACCGAGGGCGGTTATCGCTGCATCTGTGCCGAGGGCTACAAGCAGATGGAA; GGCATCTGTGTGAAGGAGCAGATCCCAGGTGCATTCCCCATCTTAACTGATTTAACCCCT; GAAACAACCCGACGCTGGAAGTTGGGTTCTCATCCCCACTCTACATATGTAAAAATGAAG; ATGCAGAGAGATGAAGCTACTTTCCCAGGGCTATATGGCAAGCAAGTCGCAAAGCTGGGA; TCCCAATCCAGACAGTCTGACCGTGGAACGAGACTCATACACAGTCAGCAGGCTTCTTCT; CAGAGATGA;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001031717 unedited; NTTCAGTTAAATTTGTATAGATCACTTAGGCGGCCGCGAATCGCACGAGGAGAGACGGCC; CACGGCGCCCGCGGGCTGGGGCGGTCGCTTCTTCCTTCTCCGTGGCCTACGAGGGTCCCC; AGCCTGGGTAAAGATGGCCCCATGGCCCCCGAAGGGCCTAGTCCCAGCTGTGCTCTGGGG; CCTCAGCCTCTTCCTCAACCTCCCAGGACCTATCTGGCTCCAGCCCTCTCCACCTCCCCA; GTCTTCTCCCCCGCCTCAGCCCCATCCGTGTCATACCTGCCGGGGACTGGTTGACAGCTT; TAACAAGGGCCTGGAGAGAACCATCCGGGACAACTTTGGAGGTGGAAACACTGCCTGGGA; GGAAGAGAATTTGTCCAAATACAAAGACAGTGAGACCCGCCTGGTAGAGGTGCTGGAGGG; TGTGTGCAGCAAGTCAGACTTCGAGTGCCACCGCCTGCTGGAGCTGAGTGAGGAGCTGGT; GGAGAGCTGGTGGTTTCACAAGCAGCAGGAGGCCCCGGACCTCTTCCAGTGGCTGTGCTC; AGATTCCCTGAAGCTCTGCTGCCCCGCAGGCACCTTCGGGCCCTCCTGCCTTCCCTGTCC; TGGGGGAACAGAGAGGCCCTGCGGTGGCTACGGGCAGTGTGAAGGAGAAGGGACACGAGG; GGGCAGCGGGCACTGTGACTGCCAAGCCGGCTACGGGGGTGAGGCCTGTGGCCAGTGTGG; CCTTGGCTACTTTTGAGCAGAACGCAACGCCAGCCATCTGGTATGTTCGGCTTGTTTTGG; CCCCTGTGCCCGATGCTCANGACCCTGAGAATCAAACTGTTTGCAATGCAAGAAGGGCTT; GGGCCTGCATCACCTCAAGTGTG;
  3' Read Nucleotide Sequence

  >Forward primer walk for NM_001031717 unedited; TGCTACGTGAGAGAGCAGCAGCGTGACGAAACAAGGGGGGAACCGCGGCAGCGGTGCCGG; GGGCACAAGCGCCGGACGCATGGGTGTGAGGACCAGTCCCCAGGAGAATTGCCCACCCGA; ACAGATCAAGTGCGGAAAAAACGCGACGCGGGGAGCGGGGTCCTCATCCCCACTCTACAT; ATGTAAAAATGAAAATGCAAAGAGATGAAGCTACTCTCCCAGGGCTATATGGCAAGCAAG; TCGCAAAGCTGGGACCCCAATCCAAACAGTCTGACCGTGGAACGAGACTCATACACAGTC; AGCAGGCTTCTTCTCAAAAATGACAAAAAACAAGTTGGTGGTGCTGCAGCAAATGTTCTT; TGGCATCATCATCTGTGCACTGGCCACGCTGGCTGCTAAGGGCGACTTGGTGTTCACCGC; CATCTTCATTGGGGCTGTGGCGGCCATGACTGGCTACTGGTTGTCAAAGCGCAGTGACCG; TGTGCTGGAGGGCTTCATCAAGGGCAAATAATCGCGGCCACCACCTGTAGGACCTCCTCC; CACCCACGCTGCCCCCAGAGCTTGGGCTGCCCTCCTGCTGGACACTCAGGACAGCTTGGT; TTATTTTTGAGAGTGGGGTAAGCACCCCTACCTGCCTTACAGAGCAGCCCAGGTACCCAG; GCCCGGGCAGACAAGGCCCCTGGGGTAAAAAGTAGCCCTGAAGGTGGATACCATGAGCTC; TTCACCTGGCGGGGACTGGCAGGCTTCACAATGTGTGAATTTCAAAAGTTTTTCCTTAAT; GGTGGCTGCTAGAGCTTTGGCCCTGCTAGATAGGTGGTCCTCACAGGGTGGGGCATCACA; GCTCTCTGCAGCTGCATGCTGCAGTCTGTCTGTGTCACCACATCCCACCCATGGCCACTT; ATTATTCATCTCAGAATAAGAAGTCTGCAG;
• Restriction Sites: NotI-NotI
• ACCN: NM_001031717
• Insert Size: 2300 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001031717.2, NP_001026887.1
• RefSeq Size: 2418 bp
• RefSeq ORF: 1269 bp
• Locus ID: 78987
• UniProt ID: Q96HD1
• Cytogenetics: 3p25.3
• Protein Families: Transmembrane
• Summary: This gene encodes a member of a subfamily of epidermal growth factor-related proteins. The encoded protein is characterized by a cysteine-rich with epidermal growth factor-like domain. This protein may function as a cell adhesion molecule. Mutations in this gene are the cause of atrioventricular septal defect. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Apr 2010]; Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.